公开(公告)号：US12150984B2

公开(公告)日：2024-11-26

申请号：US17244642

申请日：2021-04-29

Applicant: General Electric Company

Inventor： Christopher Michael Puleo ,  Victoria Eugenia Cotero ,  John Richard Nelson

IPC: C12N13/00 ,  A61K39/215 ,  C07K14/54 ,  C12N15/86 ,  A61K39/00

Abstract: The subject matter of the present disclosure generally relates to techniques for addressing or correcting dysregulation of the trauma regulation pathway. The dysregulation may be associated with a physiological condition, such as a SARS-CoV-2 viral infection. In an embodiment, the techniques include treating dysregulation based on a renin-angiotensin pathway molecule or cell and/or a splenic pathway molecule or cell using targeted neuromodulation. In an embodiment, neuromodulation is used to regulate the immune system, e.g., as an energy-based adjuvant for a vaccine.

公开(公告)号：US20210055297A1

公开(公告)日：2021-02-25

申请号：US17090383

申请日：2020-11-05

Applicant: General Electric Company

Inventor： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  Brian Christopher Bales ,  David Roger Moore ,  Paul Michael Smigelski

IPC: G01N33/569 ,  G01N1/40 ,  G01N33/543 ,  B01L3/00 ,  B01D15/36

Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.

公开(公告)号：US10830760B2

公开(公告)日：2020-11-10

申请号：US15848302

申请日：2017-12-20

Applicant: General Electric Company ,  TOKITAE LLC

Inventor： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  David Roger Moore ,  Paul Michael Smigelski, Jr. ,  John Thomas Connelly ,  Benjamin David Grant ,  Bernhard Hans Weigl

IPC: G01N33/493 ,  C07K1/22 ,  B01D15/08 ,  G01N33/50 ,  G01N33/569 ,  B01D63/02 ,  A61B10/00 ,  C12M1/26 ,  G01N33/558 ,  G01N15/14

Abstract: A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

公开(公告)号：US10598577B2

公开(公告)日：2020-03-24

申请号：US15639511

申请日：2017-06-30

Applicant: General Electric Company ,  University of Akron

Inventor： Arunkumar Natarajan ,  John Richard Nelson ,  Patrick McCoy Spooner ,  Ralf Lenigk ,  Wei Gao ,  Kwok Pong Chan ,  Lakshmi Sireesha Kaanumalle ,  Abraham Joy ,  Nicholas Nun

IPC: G01N1/44 ,  C08F2/48 ,  C12N15/10 ,  G01N33/543 ,  C08F8/50 ,  G01N1/02

Abstract: A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.

公开(公告)号：US20190062795A1

公开(公告)日：2019-02-28

申请号：US15941057

申请日：2018-03-30

Applicant: General Electric Company

Inventor： Robert Scott Duthie ,  John Richard Nelson ,  Anuradha Sekher

IPC: C12P19/34 ,  C12Q1/6848 ,  C12Q1/6844

Abstract: Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.

公开(公告)号：US20180361317A1

公开(公告)日：2018-12-20

申请号：US16012147

申请日：2018-06-19

Applicant: General Electric Company

Inventor： Christopher Michael Puleo ,  John Richard Nelson ,  Patrick McCoy Spooner ,  Ralf Lenigk ,  Nicole Lea Wood ,  Li Zhu ,  Craig Patrick Galligan

IPC: B01D57/02 ,  C12N15/10

Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.

公开(公告)号：US20160002715A1

公开(公告)日：2016-01-07

申请号：US14321235

申请日：2014-07-01

Applicant: General Electric Company

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Christopher Michael Puleo ,  Patrick McCoy Spooner

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C12Q2531/119 ,  C12Q2531/125 ,  C12Q2565/518

Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; applying an aqueous buffer to the sample application zone of the substrate to washes away one or more inhibitors present on the sample application zone; and applying an isothermal nucleic acid amplification reaction mixture to the sample application zone to amplify the target nucleic acid to form a nucleic acid amplification product. The target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight.

Abstract translation: 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 将水性缓冲液施加到基材的样品施加区域以洗涤存在于样品施加区上的一种或多种抑制剂; 以及将等温核酸扩增反应混合物应用于样品施用区域以扩增靶核酸以形成核酸扩增产物。 具有第一分子量的靶核酸基本上固定在样品施用区，并且其中扩增产物具有第二分子量。

公开(公告)号：US20150176056A1

公开(公告)日：2015-06-25

申请号：US14140127

申请日：2013-12-24

Applicant: General Electric Company

Inventor： Bing Li ,  David Roger Moore ,  William Christopher Alberts ,  John Richard Nelson

IPC: C12Q1/68 ,  G01N33/543

CPC classification number: C12Q1/6806 ,  G01N33/54346 ,  G01N33/54393

Abstract: An electrospinning approach is disclosed for generating a dissolvable formulation of a reagent of interest in a nanoscale fiber medium. In one embodiment, the nanoscale fibers can incorporate and stabilize biological agents of interest, such as for storage at room temperature for extended periods. In one implementation, the fibers can be produced in a continuous manner and dissolve rapidly.

Abstract translation: 公开了一种静电纺丝方法，用于在纳米级纤维介质中产生感兴趣的试剂的可溶性制剂。 在一个实施方案中，纳米级纤维可以掺入并稳定感兴趣的生物试剂，例如在室温下长时间储存​​。 在一个实施方案中，纤维可以以连续的方式生产并迅速溶解。

公开(公告)号：US20150080226A1

公开(公告)日：2015-03-19

申请号：US14030386

申请日：2013-09-18

Applicant: General Electric Company

Inventor： Nandini Nagraj ,  Radislav Alexandrovich Potyrailo ,  Andrew David Pris ,  John Richard Nelson

IPC: C12N15/10 ,  G01N33/53

CPC classification number: C12N15/1034 ,  C12N15/1048 ,  C12Q1/6811 ,  C40B30/04 ,  G01N33/53 ,  G01N33/5308 ,  H04N1/32309 ,  H04N1/3232 ,  H04N1/32352 ,  H04N1/40 ,  H04N1/60 ,  H04N2201/0094 ,  H04N2201/3233 ,  H04N2201/327 ,  H04N2201/3271 ,  H04N2209/00 ,  C12Q2525/205 ,  C12Q2531/125 ,  C12Q2537/162 ,  C12Q2565/107

Abstract: Methods for selecting a binding-element are provided. The method comprised of different steps. A first mixture is formed using at least one target molecule and a plurality of oligomers, followed by incubating the first mixture to form a second mixture comprising at least one target-bound oligomer and at least one target-unbound oligomer. Then a first accelerator is added to cleave the target-unbound oligomer and the target-bound oligomer is separated from the target molecule. This is followed by addition of a second accelerator for ligation, and a third accelerator for amplification followed by sequencing and post sequence analysis to select the binding-element.

Abstract translation: 提供了选择结合元件的方法。 该方法由不同的步骤组成。 使用至少一种靶分子和多个低聚物形成第一混合物，随后温育第一混合物以形成包含至少一种靶结合的低聚物和至少一种靶未结合的低聚物的第二混合物。 然后加入第一促进剂以切割目标未结合的寡聚物，并且靶向寡聚体与目标分子分离。 然后加入第二种用于连接的促进剂，和第三种用于扩增的加速剂，然后进行测序和后序列分析以选择结合元件。

公开(公告)号：US20140154736A1

公开(公告)日：2014-06-05

申请号：US13690801

申请日：2012-11-30

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Christopher Michael Puleo ,  John Richard Nelson ,  Patrick McCoy Spooner ,  Ralf Lenigk

IPC: G01N1/30 ,  G01N1/31

CPC classification number: G01N1/30 ,  A01N1/0242 ,  A01N1/0284 ,  B01L3/5023 ,  B01L3/5088 ,  B01L7/00 ,  B01L2200/0678 ,  B01L2300/0825 ,  B01L2300/126 ,  B01L2300/1877 ,  G01N2001/4027 ,  Y10T436/2525

Abstract: A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.

Abstract translation: 提供了一种干燥设置在基板上的生物样品的方法。 该方法包括提供包括样品加载区域和热源的基底; 激活热源产生热量; 将衬底加热至少高于65℃; 并干燥生物样品。 还提供了用于存储样品的装置，其中所述装置包括用于生物样品储存的基底; 以及产生热量以保持至少高于65℃的温度的加热组分。加热组分可以含有一种或多种试剂，其中试剂产生热量以保持至少高于65℃的温度。