公开(公告)号：US20230250378A1

公开(公告)日：2023-08-10

申请号：US18012613

申请日：2020-07-08

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Fausto D'Apuzzo ,  Viktor Shkolnikov ,  Alexander N. Govyadinov

IPC: C12M3/06 ,  C12M1/42 ,  C12M1/34 ,  G01N15/14

CPC classification number: C12M23/16 ,  C12M35/04 ,  C12M41/40 ,  G01N15/1463 ,  G01N15/1459 ,  G01N2015/1495 ,  G01N2015/1006

Abstract: An example method for measuring deformability of a cell responsive to a pressure wave, consistent with the present disclosure, includes moving a cell of a sample into a cell probing chamber of a microfluidic device. While the cell is in the cell probing chamber, the method includes generating a pressure wave within the cell probing chamber by actuating a fluidic pump. The method further includes determining a deformability of the cell responsive to the pressure wave, using an imaging array synchronized with the actuation of the fluidic pump.

公开(公告)号：US20170176318A1

公开(公告)日：2017-06-22

申请号：US15388582

申请日：2016-12-22

Applicant: Canon U.S. Life Sciences, Inc. ,  University of Maryland, College Park

Inventor： Giuliano Scarcelli ,  Jitao Zhang ,  Antonio Fiore ,  Hanyoup Kim

IPC: G01N15/14

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1495 ,  G01N2021/638

Abstract: The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.

公开(公告)号：US20170016882A1

公开(公告)日：2017-01-19

申请号：US15278732

申请日：2016-09-28

Applicant: Satish DESHPANDE

Inventor： Satish DESHPANDE ,  James Andrew ESTILL ,  Donald Franklin MOYER

IPC: G01N33/50 ,  G01N21/49 ,  G01N15/14

CPC classification number: G01N33/5026 ,  G01N15/0211 ,  G01N21/51 ,  G01N33/49 ,  G01N33/5044 ,  G01N33/5091 ,  G01N33/5094 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1495 ,  G01N2015/1497 ,  G01N2021/4707 ,  G01N2021/4711 ,  G01N2021/4723

Abstract: Early warning of changing health and robustness is given by tracking of ease of morphological changes in blood cells obtained by comparing intensities in a first scattered light intensity angular distribution and intensities in a second scattered light intensity angular distribution, with the light being scattered by blood cells into very narrowly forward scattered light intensity angular range.

Abstract translation: 通过跟踪通过比较第一散射光强度角分布中的强度和第二散射光强度角分布中的强度获得的血细胞的形态学变化的容易程度给出对健康和健壮性的改变的预警，其中光被血细胞散射 进入非常狭窄的前方散射光强度范围。

公开(公告)号：US09487812B2

公开(公告)日：2016-11-08

申请号：US13770875

申请日：2013-02-19

Applicant: Colorado School of Mines

Inventor： Keith B. Neeves ,  David W. M. Marr ,  Kevin B. Roth ,  Charles D. Eggleton

IPC: B01L99/00 ,  G01N15/14 ,  C12Q1/02 ,  B01L3/00

CPC classification number: C12Q1/02 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0463 ,  B01L2400/0487 ,  G01N15/1484 ,  G01N2015/1495

Abstract: A microfluidic system, device, and method are disclosed. The microfluidic system may include a first microfluidic channel and a second microfluidic channel, each of which are carrying one or more objects. There is an intersection between the first and second microfluidic channels where one or more objects from the first microfluidic channel impact one or more objects from the second microfluidic channel under hydrodynamic forces. The impact causes the objects to deform and the deformation of objects can be analyzed to determine properties of the object.

Abstract translation: 公开了微流体系统，装置和方法。 微流体系统可以包括第一微流体通道和第二微流体通道，每个通道都携带一个或多个物体。 在第一和第二微流体通道之间存在交叉点，其中来自第一微流体通道的一个或多个物体在流体动力作用下冲击来自第二微流体通道的一个或多个物体。 冲击会导致物体变形，并且可以分析物体的变形以确定物体的属性。

公开(公告)号：US09090865B2

公开(公告)日：2015-07-28

申请号：US13284781

申请日：2011-10-28

Applicant: Dino Di Carlo ,  Soojung Hur

Inventor： Dino Di Carlo ,  Soojung Hur

IPC: C12M1/00 ,  G01N15/10 ,  B01L3/00

CPC classification number: G01N15/14 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0864 ,  B01L2400/0487 ,  C12M47/04 ,  G01N15/10 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/105 ,  G01N2015/1081 ,  G01N2015/1087 ,  G01N2015/1495

Abstract: A particle analysis system includes an inlet; an inertial focusing microchannel disposed in a substrate and having a downstream expanding region at a distal end, where the inlet is connected to a proximal end of the microchannel; a plurality of outlets connected to the microchannel at the downstream expanding region; a plurality of fluidic resistors, where each fluidic resistor is connected to a respective outlet; and a particle analyzer configured to measure a size and a position of particles in the microchannel. A particle sorting system includes an inlet; an inertial focusing microchannel disposed in a substrate and having a downstream expanding region at a distal end, where the inlet is connected to a proximal end of the microchannel; a plurality of outlets connected to the microchannel at the downstream expanding region; and a plurality of fluidic resistors, where each fluidic resistor is connected to a respective outlet.

Abstract translation: 粒子分析系统包括入口; 惯性聚焦微通道，其设置在基底中并且在远端具有下游扩张区域，其中所述入口连接到所述微通道的近端; 在下游扩展区域连接到微通道的多个出口; 多个流体电阻器，其中每个流体电阻器连接到相应的出口; 以及粒子分析器，被配置为测量微通道中颗粒的尺寸和位置。 颗粒分选系统包括入口; 惯性聚焦微通道，其设置在基底中并且在远端具有下游扩张区域，其中所述入口连接到所述微通道的近端; 在下游扩展区域连接到微通道的多个出口; 和多个流体电阻器，其中每个流体电阻器连接到相应的出口。

公开(公告)号：US08935098B2

公开(公告)日：2015-01-13

申请号：US13823109

申请日：2011-09-16

Applicant: Dino Di Carlo ,  Daniel R. Gossett ,  Henry T. K. Tse

Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T. K. Tse

IPC: G01N33/483 ,  C12M1/34 ,  G01N1/30 ,  G01N15/14 ,  G06F19/10 ,  G01N33/574 ,  C12Q1/02

CPC classification number: G01N15/1463 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502776 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0864 ,  C12Q1/02 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/487 ,  G01N33/574 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/149 ,  G01N2015/1495 ,  G01N2015/1497 ,  G01N2800/7028 ,  G06F19/10

Abstract: A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.

Abstract translation: 公开了一种能够以高产量自动测量细胞机械参数的系统。 微流体装置使用相交流来产生细胞经受受控拉伸的拉伸流动区域。 在进入延伸流动区域之前，细胞被聚焦成流线。 在拉伸区域中，每个单元的变形用成像装置测量。 自动图像分析从图像中提取一系列独立的生物力学参数。 这些可能包括细胞大小，变形能力和圆形度。 然后可以以各种方式使用所获得的单细胞数据。 可以为用户开发和显示可变形性和圆形度的散射密度图。 诸如可变形性和圆形度的机械参数可以被门控或阈值化以识别感兴趣的某些细胞或感兴趣的亚群。 类似地，使用该设备获得的机械数据可以用作单元签名。

公开(公告)号：US20090279743A1

公开(公告)日：2009-11-12

申请号：US12116012

申请日：2008-05-06

Applicant: Kecheng Li ,  Dongbo Yan

Inventor： Kecheng Li ,  Dongbo Yan

IPC: G06K9/00

CPC classification number: G01N33/343 ,  G01N15/1463 ,  G01N2015/1493 ,  G01N2015/1495 ,  G01N2015/1497

Abstract: A method for measuring a property of a fibre, such as flexibility, collapsibility and moment of inertia. A fibre is wetted and deformed in its wet state, and an optical section image of the deformed fibre is taken. A measurement is made on the image and the desired property is calculated using the measurement.

Abstract translation: 用于测量纤维的性质的方法，例如柔性，可收缩性和惯性矩。 纤维在其湿润状态下被润湿和变形，并且获得变形纤维的光学截面图像。 对图像进行测量，并使用测量计算所需的性质。

公开(公告)号：US20070008528A1

公开(公告)日：2007-01-11

申请号：US11175140

申请日：2005-07-07

Applicant: Arthur Chiou ,  Chi-Hung Lin ,  Artashes Karmenyan ,  I Hsiao

Inventor： Arthur Chiou ,  Chi-Hung Lin ,  Artashes Karmenyan ,  I Hsiao

IPC: G01N21/00 ,  G01B11/24

CPC classification number: G01N15/1475 ,  G01N2015/1493 ,  G01N2015/1495

Abstract: The present invention provides a method and device for simultaneous optical trapping, stretching, and measurement of morphological deformation of a micro-particle in real-time. Using the setup of the present invention, the deformability of a living cell can be obtained in real-time by measuring the variation in coupling efficiency with optical power of light coupled from one single-mode fiber to the other through the lensing effect of the trapped-and-stretched micro-particle.

Abstract translation: 本发明提供了一种用于同时光学捕获，拉伸和测量微粒形态变形的方法和装置。 利用本发明的设置，可以通过测量由一个单模光纤耦合到另一个单模光纤的光的光功率的耦合效率的变化，通过被捕获的透镜效应来实时获得活细胞的变形能力 并拉伸微粒。

公开(公告)号：US06927065B2

公开(公告)日：2005-08-09

申请号：US09875779

申请日：2001-06-06

Applicant: Eugene Y. Chan ,  Lance C. Gleich ,  Parris S. Wellman

Inventor： Eugene Y. Chan ,  Lance C. Gleich ,  Parris S. Wellman

IPC: B01F5/06 ,  B01F13/00 ,  B01L3/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N1/28 ,  G01N15/14 ,  G01N21/64 ,  G01N21/78 ,  G01N27/447 ,  G01N33/44 ,  G01N33/483

CPC classification number: B01L3/502761 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2200/0663 ,  B01L2300/0816 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N1/286 ,  G01N15/1456 ,  G01N15/1459 ,  G01N15/1463 ,  G01N33/442 ,  G01N2015/1075 ,  G01N2015/1493 ,  G01N2015/1495 ,  Y10T436/13 ,  Y10T436/143333

Abstract: A method for determining the length and velocity of single elongated macromolecules is disclosed. In particular, the present invention relates to methods and apparatus for determining the velocity of elongated polymeric molecules moving relative to one or more detection stations, as well as to methods and apparatus for determining the length of such molecules and the distance between landmarks that may be present on such molecules. The invention makes use of time correlated measurements of signal amplitude profiles that result from interactions between each detection station and portions of each macromolecule.

Abstract translation: 公开了一种用于确定单个细长大分子的长度和速度的方法。 特别地，本发明涉及用于确定相对于一个或多个检测站移动的细长聚合物分子的速度的方法和装置，以及用于确定这种分子的长度的方法和装置以及标记之间的距离 存在于这样的分子上。 本发明利用由每个检测站与每个大分子的部分之间的相互作用产生的信号幅度分布的时间相关测量。

公开(公告)号：US06460399B1

公开(公告)日：2002-10-08

申请号：US09712034

申请日：2000-11-14

Applicant: Willem Johan Beekman ,  Gabriel M. H. Meesters ,  Brian Scarlett

Inventor： Willem Johan Beekman ,  Gabriel M. H. Meesters ,  Brian Scarlett

IPC: G01M700

CPC classification number: G01N3/40 ,  G01N15/1463 ,  G01N2015/0096 ,  G01N2015/1495 ,  G01N2015/1497 ,  G01N2033/0077 ,  G01N2203/0019 ,  G01N2203/0087 ,  G01N2203/0623 ,  G01N2203/0676

Abstract: A method and apparatus for the accurate monitoring of a granule during compression testing is provided and enables the study of breakage mechanisms that cause particle failure during compression. The method involves compressing a granule or particle with the apparatus and the apparatus is a spring device that combines a spring in parallel with the granule or particle and a spring in series with the granule or particle. Under failure conditions, sudden deformation of the granule or particle results in fast relaxation of the granule or particle during breakage and allows very careful granule breakage. Measuring or recording devices coupled to the apparatus provide for the detailed study of breakage conditions at the moment of failure and lend incite into the compression strength characteristics of the granule or particle.

Abstract translation: 提供了一种用于在压缩试验期间精确监测颗粒的方法和装置，并且能够研究在压缩期间引起颗粒失效的断裂机制。 该方法包括用设备压缩颗粒或颗粒，并且该装置是弹簧装置，其将与颗粒或颗粒平行的弹簧和与颗粒或颗粒串联的弹簧组合。 在故障条件下，颗粒或颗粒的突然变形导致颗粒或颗粒在破裂过程中快速松弛，并允许非常小心的颗粒破裂。 耦合到该装置的测量或记录装置提供了在故障时对破裂条件的详细研究，并且煽动颗粒或颗粒的压缩强度特性。