公开(公告)号：US20230364601A1

公开(公告)日：2023-11-16

申请号：US18225981

申请日：2023-07-25

Applicant: UNIVERSITY OF PUERTO RICO

Inventor： Jose Lasalde-Dominicci ,  Orestes Quesada-Gonzalez ,  Josue Rodriguez-Cordero ,  Carlos Baez-Pagan ,  Martin MONTOYA-ZAVALA

IPC: B01L3/06 ,  C30B7/12 ,  C40B40/10 ,  B01L3/00 ,  C07K1/30 ,  C30B29/58 ,  B01J19/00 ,  B01J19/18

CPC classification number: B01L3/06 ,  C30B7/12 ,  C40B40/10 ,  B01L3/5085 ,  C07K1/306 ,  C30B29/58 ,  B01J19/0046 ,  B01J19/1893 ,  B01J2219/00725 ,  B01J2219/00522 ,  B01J2219/00756 ,  B01J2219/00317 ,  B01J2219/00639 ,  B01L2300/069 ,  B01J2219/00585 ,  B01J2219/00745

Abstract: The invention is a high-throughput voltage screening crystallographic device and methodology that uses multiple micro wells and electric circuits capable of assaying different crystallization condition for the same or different proteins of interest at the same of different voltages under a humidity and temperature controlled environment. The protein is solubilized in a lipid matrix similar to the lipid composition of the protein in the native environment to ensure stability of the protein during crystallization. The invention provides a system and method where the protein is transferred to a lipid matrix that holds a resting membrane potential, which reduces the degree of conformational freedom of the protein. The invention overcomes the majority of the difficulties associated with vapor diffusion techniques and essentially reconstitutes the protein in its native lipid environment under “cuasi” physiological conditions.

公开(公告)号：US11643751B2

公开(公告)日：2023-05-09

申请号：US17198204

申请日：2021-03-10

Applicant: MATRIX SENSORS, INC.

Inventor： David K Britt ,  Paul R Wilkinson ,  Steven Yamamoto

IPC: C30B28/04 ,  C08J5/18 ,  C07F15/04 ,  G01G3/16 ,  C30B29/58 ,  C07F9/00

CPC classification number: C30B28/04 ,  C07F9/00 ,  C07F15/04 ,  C08J5/18 ,  C30B29/58 ,  G01G3/165

Abstract: An apparatus and method is provided for coating a surface of a material with a film of porous coordination polymer. A first substrate having a first surface to be coated is positioned in a processing chamber such that the first surface is placed in a substantially opposing relationship to a second surface. In some embodiments, the second surface is provided by a wall of the processing chamber, and in other embodiments the second surface is provided by a second substrate to be coated. The first substrate is held such that a gap exists between the first and second surfaces, and the gap is filled with at least one reaction mixture comprising reagents sufficient to form the crystalline film on at least the first surface. A thin gap (e.g., having a thickness less than 2 mm) between the first and second surfaces is effective for producing a high quality film having a thickness less than 100 μm. Confining the volume of the reaction mixture to a thin layer adjacent the substrate surface significantly reduces problems with sedimentation and concentration control. In some embodiments, the size, shape, or average thickness of the gap is adjusted during formation of the film in response to feedback from at least one film growth monitor.

公开(公告)号：US09821325B2

公开(公告)日：2017-11-21

申请号：US14780766

申请日：2014-04-28

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Uwe Weierstall ,  John Spence ,  R. Bruce Doak

IPC: B05B1/02 ,  B05B1/10 ,  B05B7/06 ,  B05B7/08 ,  C30B7/00 ,  C30B29/58 ,  B05B7/24 ,  C30B7/02

CPC classification number: B05B1/10 ,  B05B1/02 ,  B05B7/064 ,  B05B7/066 ,  B05B7/08 ,  B05B7/24 ,  C30B7/00 ,  C30B7/02 ,  C30B29/58

Abstract: An injector apparatus and methods for use, where the injector apparatus comprises: (a) hydraulic stage having first and second ends and including a housing defining a cavity, a primary plunger disposed in the cavity and a secondary plunger, (b) a pressurization system coupled to the hydraulic stage's first end, where the primary plunger is in fluid communication with the pressurization system and is in mechanical communication with the secondary plunger, (c) a reservoir bore defined in the hydraulic stage housing and configured to receive the primary plunger's second end, where the secondary plunger is disposed within the reservoir bore and (d) a nozzle assembly including a housing, a gas tube and a nozzle capillary, where the nozzle capillary is partially disposed in and is substantially coaxial with the gas tube, where the nozzle capillary's first end is in fluid communication with the reservoir bore's second end.

公开(公告)号：US09810980B1

公开(公告)日：2017-11-07

申请号：US15426523

申请日：2017-02-07

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION ,  TOKYO ELECTRON LIMITED

Inventor： Hongyun Cottle ,  Cheng Chi ,  Chi-Chun Liu ,  Kristin Schmidt

IPC: C03C15/00 ,  G03F7/00 ,  C30B1/04 ,  C30B29/58 ,  H01L21/027 ,  H01L21/02 ,  H01L21/311 ,  H01L21/3105

CPC classification number: G03F7/0002 ,  B81C1/00031 ,  B81C1/00396 ,  B81C1/00531 ,  B81C2201/0149 ,  C09K13/00 ,  C30B1/04 ,  C30B7/005 ,  C30B29/58 ,  C30B33/08 ,  H01L21/02118 ,  H01L21/0271 ,  H01L21/302 ,  H01L21/31058 ,  H01L21/31133

Abstract: Graphoepitaxy directed self-assembly methods generally include grafting a conformal layer of a polymer brush onto a topographic substrate. A planarization material, which functions as a sacrificial material is coated onto the topographic substrate. The planarization material is etched back to a top surface of the topographic substrate, wherein the etch back removes the polymer brush from the top surfaces of the topographic substrate. The remaining portion of the polymer brush is protected by the remaining planarization material below the top surface of the topographic substrate, which can be removed with a solvent to provide the topographic substrate with a conformal polymer brush below the top surface of the topographic substrate. The substrate is then coated with a block copolymer and annealed to direct self-assembly of the block copolymer. The methods mitigate island and/or hole defect formation.

公开(公告)号：US20160370306A1

公开(公告)日：2016-12-22

申请号：US15183149

申请日：2016-06-15

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Chelsie Conrad ,  Petra Fromme ,  Daniel James ,  Christopher Kupitz

IPC: G01N23/20 ,  G01N23/207 ,  C30B29/58 ,  G01N33/483 ,  C30B7/14

CPC classification number: G01N23/2005 ,  C30B7/14 ,  C30B29/58 ,  G01N33/483

Abstract: A system and method for preparing a crystal delivery medium comprising agarose for serial femtosecond crystallography and uses thereof.

Abstract translation: 一种用于制备包含用于连续飞秒晶体学的琼脂糖的晶体输送介质的系统和方法及其用途。

公开(公告)号：US20160215411A1

公开(公告)日：2016-07-28

申请号：US14517602

申请日：2014-10-17

Applicant: The University of Chicago

Inventor： Rustem F. Ismagilov ,  Joshua David Tice ,  Cory John Gerdts ,  Bo Zheng

IPC: C30B7/14 ,  B01D9/00 ,  C30B29/54 ,  C30B29/58 ,  C12N9/36 ,  C07K14/43

CPC classification number: B01L3/502784 ,  B01D9/0072 ,  B01D2009/0086 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0071 ,  B01J14/00 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00736 ,  B01J2219/00756 ,  B01J2219/00783 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00894 ,  B01J2219/00903 ,  B01J2219/00975 ,  B01J2219/00977 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/0673 ,  B01L2200/12 ,  B01L2300/0867 ,  B01L2400/0487 ,  B82Y30/00 ,  C07K14/43 ,  C12N9/2462 ,  C12Q1/6806 ,  C12Y302/01017 ,  C30B7/14 ,  C30B29/54 ,  C30B29/58 ,  G01N1/28 ,  G01N35/08 ,  Y10T117/10 ,  Y10T137/0318 ,  Y10T436/117497 ,  Y10T436/143333 ,  Y10T436/25

Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

公开(公告)号：US20160208405A1

公开(公告)日：2016-07-21

申请号：US14914755

申请日：2014-08-27

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Yoko YAMANISHI ,  Fumihito ARAI ,  Hiroki KURIKI

IPC: C30B7/12 ,  C07K1/30 ,  C30B33/04 ,  C12N9/06 ,  C30B29/58 ,  C30B30/02 ,  C07K14/80 ,  C12N9/36

CPC classification number: C30B7/12 ,  C07K1/306 ,  C07K14/80 ,  C12N9/0016 ,  C12N9/2462 ,  C12Y104/01014 ,  C30B29/58 ,  C30B30/02 ,  C30B33/04

Abstract: Provided are a protein crystal device and method for crystallizing protein capable of generating protein crystal without imparting a heat effect, a protein crystal-cutting device and method for cutting protein crystal capable of cutting protein crystal without imparting a heat effect on protein crystal, and bubble-jetting member and protein-adsorbing-bubble-jetting member used in said device. A bubble-jetting member is used in a protein crystal device to jet bubbles into a protein solution to thereby allow protein crystals to be obtained, the bubble-jetting member comprising: a core formed of a conductive material; a shell part formed of an insulating material, including an extended section extending from the tip of the core, and in which at least a portion closely adheres to the core to cover the core; and a gap having a bubble-jetting port, the gap being formed between the extended section and the tip of the core.

Abstract translation: 本发明提供一种蛋白质晶体装置和结晶蛋白质的方法，该蛋白质能够产生不产生热效应的蛋白质晶体，蛋白质晶体切割装置和切割能够切割蛋白质晶体的蛋白质晶体而不会对蛋白质晶体产生热效应的蛋白质晶体和方法 - 喷射构件和蛋白质吸附 - 气泡喷射构件。 在蛋白质晶体装置中使用气泡喷射构件将气泡喷射到蛋白质溶液中，从而获得蛋白质晶体，气泡喷射构件包括：由导电材料形成的芯; 由绝缘材料形成的壳体部分，包括从所述芯的尖端延伸的延伸部分，并且其中至少一部分紧密地附着到所述芯部以覆盖所述芯部; 以及具有气泡喷射口的间隙，所述间隙形成在所述延伸部和所述芯的尖端之间。

公开(公告)号：US09284659B2

公开(公告)日：2016-03-15

申请号：US13806149

申请日：2011-06-30

Applicant: Christian Betzel ,  Rolf Hilgenfeld ,  Dierk Hilterhaus ,  Thomas Klupsch ,  Peter Muehlig ,  Karsten Dierks ,  Arne Meyer

Inventor： Christian Betzel ,  Rolf Hilgenfeld ,  Dierk Hilterhaus ,  Thomas Klupsch ,  Peter Muehlig ,  Karsten Dierks ,  Arne Meyer

IPC: C30B29/54 ,  C30B7/02 ,  C30B7/08 ,  C30B7/00 ,  C30B29/58

CPC classification number: C30B7/02 ,  C30B7/00 ,  C30B7/08 ,  C30B29/54 ,  C30B29/58 ,  Y10T117/1008

Abstract: A method for crystallizing a substance dissolved in a solvent, including the following steps: introducing a solvent volume containing the substance into a chamber having a preset temperature, humidity, and gas composition, adding a predetermined volume of a precipitant to the solvent volume containing the substance, allowing the solvent to evaporate while simultaneously observing structural changes in the solvent volume containing the substance and the precipitant by means of dynamic light scattering, detecting weight changes and determining the molarities, making an association with the location in the phase diagram on the basis of the DLS measurement and the results of the molarity determination, allowing a predetermined number of crystal nuclei to form by adding solvent or adding precipitant, putting the solvent volume containing the substance and the precipitant into a metastable state by adding solvent and/or protein solution or by allowing the concentration of the dissolved substance to decrease by allowing nucleation cores to form, maintaining the metastable state by adding a predetermined amount of the substance to the solvent volume containing the substance and the precipitant or allowing the solvent to evaporate until at least one crystal of a predetermined size is formed.

Abstract translation: 一种使溶解在溶剂中的物质结晶的方法，包括以下步骤：将含有物质的溶剂体积引入具有预设温度，湿度和气体组成的室中，将预定体积的沉淀剂加入到含有 物质，允许溶剂蒸发，同时通过动态光散射同时观察包含物质和沉淀剂的溶剂体积的结构变化，检测重量变化并确定摩尔数，在相图中与位置相关联 的DLS测量结果和摩尔浓度测定结果，通过加入溶剂或加入沉淀剂形成预定数量的晶核，通过加入溶剂和/或蛋白质溶液将含有物质和沉淀剂的溶剂体积放入亚稳态 或通过允许浓度溶解的子 通过允许成核核心形成，通过向含有物质和沉淀剂的溶剂体积中加入预定量的物质或使溶剂蒸发直到形成至少一种预定尺寸的晶体来保持亚稳态。

公开(公告)号：US20160051995A1

公开(公告)日：2016-02-25

申请号：US14780766

申请日：2014-04-28

Applicant: John SPENCE ,  Bruce R. DOAK ,  ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Uwe WEIERSTALL ,  John SPENCE ,  R. Bruce DOAK

IPC: B05B1/10 ,  B05B7/06 ,  B05B7/24 ,  C30B7/02 ,  C30B29/58

CPC classification number: B05B1/10 ,  B05B1/02 ,  B05B7/064 ,  B05B7/066 ,  B05B7/08 ,  B05B7/24 ,  C30B7/00 ,  C30B7/02 ,  C30B29/58

Abstract: An injector apparatus and methods for use, where the injector apparatus comprises: (a) hydraulic stage having first and second ends and including a housing defining a cavity, a primary plunger disposed in the cavity and a secondary plunger, (b) a pressurization system coupled to the hydraulic stage's first end, where the primary plunger is in fluid communication with the pressurization system and is in mechanical communication with the secondary plunger, (c) a reservoir bore defined in the hydraulic stage housing and configured to receive the primary plunger's second end, where the secondary plunger is disposed within the reservoir bore and (d) a nozzle assembly including a housing, a gas tube and a nozzle capillary, where the nozzle capillary is partially disposed in and is substantially coaxial with the gas tube, where the nozzle capillary's first end is in fluid communication with the reservoir bore's second end.

Abstract translation: 一种注射器装置和使用方法，其中所述注射器装置包括：（a）具有第一和第二端并且包括限定空腔的壳体的液压台，设置在所述空腔中的主柱塞和次级柱塞，（b）加压系统 耦合到液压级的第一端，其中主柱塞与加压系统流体连通并且与次级柱塞机械连通，（c）限定在液压级壳体中的储存器孔，并且构造成容纳主柱塞的第二端 （d）喷嘴组件，其包括壳体，气体管和喷嘴毛细管，其中喷嘴毛细管部分地设置在气体管中并基本上与气体管同轴，其中， 喷嘴毛细管的第一端与储存器孔的第二端流体连通。

公开(公告)号：US09181632B1

公开(公告)日：2015-11-10

申请号：US14479735

申请日：2014-09-08

Applicant: Merck Sharp & Dohme Corp.

Inventor： Nicholas Murgolo ,  Lorraine Hernandez ,  Payal Sheth ,  Corey Strickland ,  Peter Orth ,  Alex Therien ,  Paul Reichert ,  Li Xiao

IPC: C30B29/58 ,  C07K16/12 ,  C12N9/10 ,  C30B7/04

CPC classification number: C07K14/33 ,  C07K16/1282 ,  C07K2299/00 ,  C07K2317/21 ,  C07K2317/55 ,  C07K2317/76 ,  C12N9/1051 ,  C30B7/00 ,  C30B7/04 ,  C30B29/58

Abstract: The present invention provides polypeptide domains of C. difficile toxin B (B1, B2, B3, B4) and complexes between the polypeptides and antibodies that bind specifically for the polypeptide. Methods of using the polypeptides to generate antibodies are also provided.

Abstract translation: 本发明提供艰难梭菌毒素B（B1，B2，B3，B4）的多肽结构域以及多肽与特异性结合多肽的抗体之间的复合物。 还提供了使用多肽产生抗体的方法。