公开(公告)号：US20230364601A1

公开(公告)日：2023-11-16

申请号：US18225981

申请日：2023-07-25

Applicant: UNIVERSITY OF PUERTO RICO

Inventor： Jose Lasalde-Dominicci ,  Orestes Quesada-Gonzalez ,  Josue Rodriguez-Cordero ,  Carlos Baez-Pagan ,  Martin MONTOYA-ZAVALA

IPC: B01L3/06 ,  C30B7/12 ,  C40B40/10 ,  B01L3/00 ,  C07K1/30 ,  C30B29/58 ,  B01J19/00 ,  B01J19/18

CPC classification number: B01L3/06 ,  C30B7/12 ,  C40B40/10 ,  B01L3/5085 ,  C07K1/306 ,  C30B29/58 ,  B01J19/0046 ,  B01J19/1893 ,  B01J2219/00725 ,  B01J2219/00522 ,  B01J2219/00756 ,  B01J2219/00317 ,  B01J2219/00639 ,  B01L2300/069 ,  B01J2219/00585 ,  B01J2219/00745

Abstract: The invention is a high-throughput voltage screening crystallographic device and methodology that uses multiple micro wells and electric circuits capable of assaying different crystallization condition for the same or different proteins of interest at the same of different voltages under a humidity and temperature controlled environment. The protein is solubilized in a lipid matrix similar to the lipid composition of the protein in the native environment to ensure stability of the protein during crystallization. The invention provides a system and method where the protein is transferred to a lipid matrix that holds a resting membrane potential, which reduces the degree of conformational freedom of the protein. The invention overcomes the majority of the difficulties associated with vapor diffusion techniques and essentially reconstitutes the protein in its native lipid environment under “cuasi” physiological conditions.

公开(公告)号：US11717819B2

公开(公告)日：2023-08-08

申请号：US17741595

申请日：2022-05-11

Applicant: UNIVERSITY OF PUERTO RICO

Inventor： Jose Lasalde-Dominicci ,  Orestes Quesada-Gonzalez ,  Josue Rodriguez-Cordero ,  Carlos Baez-Pagan ,  Martin Montoya-Zavala

IPC: C30B7/12 ,  B01L3/06 ,  B01J19/00 ,  C40B40/10 ,  C07K1/30 ,  C30B29/58 ,  B01J19/18 ,  B01L3/00

CPC classification number: B01L3/06 ,  B01J19/0046 ,  B01J19/1893 ,  B01L3/5085 ,  C07K1/306 ,  C30B7/12 ,  C30B29/58 ,  C40B40/10 ,  B01J2219/00317 ,  B01J2219/00522 ,  B01J2219/00585 ,  B01J2219/00639 ,  B01J2219/00725 ,  B01J2219/00745 ,  B01J2219/00756 ,  B01L2300/069

Abstract: The invention is a high-throughput voltage screening crystallographic device and methodology that uses multiple micro wells and electric circuits capable of assaying different crystallization condition for the same or different proteins of interest at the same of different voltages under a humidity and temperature controlled environment. The protein is solubilized in a lipid matrix similar to the lipid composition of the protein in the native environment to ensure stability of the protein during crystallization. The invention provides a system and method where the protein is transferred to a lipid matrix that holds a resting membrane potential, which reduces the degree of conformational freedom of the protein. The invention overcomes the majority of the difficulties associated with vapor diffusion techniques and essentially reconstitutes the protein in its native lipid environment under “cuasi” physiological conditions.

公开(公告)号：US12128399B2

公开(公告)日：2024-10-29

申请号：US18225981

申请日：2023-07-25

Applicant: UNIVERSITY OF PUERTO RICO

Inventor： Jose Lasalde-Dominicci ,  Orestes Quesada-Gonzalez ,  Josue Rodriguez-Cordero ,  Carlos Baez-Pagan ,  Martin Montoya-Zavala

IPC: C30B7/12 ,  B01J19/00 ,  B01J19/18 ,  B01L3/00 ,  B01L3/06 ,  C07K1/30 ,  C30B29/58 ,  C40B40/10

CPC classification number: B01L3/06 ,  B01J19/0046 ,  B01J19/1893 ,  B01L3/5085 ,  C07K1/306 ,  C30B7/12 ,  C30B29/58 ,  C40B40/10 ,  B01J2219/00317 ,  B01J2219/00522 ,  B01J2219/00585 ,  B01J2219/00639 ,  B01J2219/00725 ,  B01J2219/00745 ,  B01J2219/00756 ,  B01L2300/069

Abstract: The invention is a high-throughput voltage screening crystallographic device and methodology that uses multiple micro wells and electric circuits capable of assaying different crystallization condition for the same or different proteins of interest at the same of different voltages under a humidity and temperature controlled environment. The protein is solubilized in a lipid matrix similar to the lipid composition of the protein in the native environment to ensure stability of the protein during crystallization. The invention provides a system and method where the protein is transferred to a lipid matrix that holds a resting membrane potential, which reduces the degree of conformational freedom of the protein. The invention overcomes the majority of the difficulties associated with vapor diffusion techniques and essentially reconstitutes the protein in its native lipid environment under “cuasi” physiological conditions.

公开(公告)号：US11440001B1

公开(公告)日：2022-09-13

申请号：US15996946

申请日：2018-06-04

Applicant: UNIVERSITY OF PUERTO RICO

Inventor： Jose A. Lasalde-Dominicci ,  Orestes Quesada-Gonzalez ,  Josue Rodriguez-Cordero ,  Carlos Baez-Pagan ,  Martin Montoya-Zavala

IPC: C30B7/12 ,  B01L3/06 ,  B01J19/00 ,  B01L3/00 ,  C07K1/30 ,  C30B29/58 ,  C40B40/10 ,  B01J19/18

Abstract: The invention is a high-throughput voltage screening crystallographic device and methodology that uses multiple micro wells and electric circuits capable of assaying different crystallization condition for the same or different proteins of interest at the same of different voltages under a humidity and temperature controlled environment. The protein is solubilized in a lipid matrix similar to the lipid composition of the protein in the native environment to ensure stability of the protein during crystallization. The invention provides a system and method where the protein is transferred to a lipid matrix that holds a resting membrane potential, which reduces the degree of conformational freedom of the protein. The invention overcomes the majority of the difficulties associated with vapor diffusion techniques and essentially reconstitutes the protein in its native lipid environment under “cuasi” physiological conditions.

公开(公告)号：US20220274101A1

公开(公告)日：2022-09-01

申请号：US17741595

申请日：2022-05-11

Applicant: UNIVERSITY OF PUERTO RICO

Inventor： Jose Lasalde-Dominicci ,  Orestes Quesada-Gonzalez ,  Josue Rodriguez-Cordero ,  Carlos Baez-Pagan ,  Martin MONTOYA-ZAVALA

IPC: B01L3/06 ,  B01J19/00 ,  C40B40/10 ,  C07K1/30 ,  C30B7/12 ,  C30B29/58 ,  B01J19/18 ,  B01L3/00

Abstract: The invention is a high-throughput voltage screening crystallographic device and methodology that uses multiple micro wells and electric circuits capable of assaying different crystallization condition for the same or different proteins of interest at the same of different voltages under a humidity and temperature controlled environment. The protein is solubilized in a lipid matrix similar to the lipid composition of the protein in the native environment to ensure stability of the protein during crystallization. The invention provides a system and method where the protein is transferred to a lipid matrix that holds a resting membrane potential, which reduces the degree of conformational freedom of the protein. The invention overcomes the majority of the difficulties associated with vapor diffusion techniques and essentially reconstitutes the protein in its native lipid environment under “cuasi” physiological conditions.