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    Method of and apparatus for the purification of nucleic acids using immobilized metal-ligand complex
    1.
    发明申请
    Method of and apparatus for the purification of nucleic acids using immobilized metal-ligand complex 审中-公开
    使用固定化金属 - 配体复合物纯化核酸的方法和设备

    公开(公告)号：US20060275815A1

    公开(公告)日：2006-12-07

    申请号：US11446795

    申请日：2006-06-05

    申请人： Chang-eun Yoo ,  Sang-hyun Peak ,  Sook-young Kim ,  Jong-myeon Park

    发明人： Chang-eun Yoo ,  Sang-hyun Peak ,  Sook-young Kim ,  Jong-myeon Park

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C07H21/04 ,  C12N15/1006

    摘要： Provided is a method of purifying nucleic acids using a metal-ligand complex, the method comprising: immobilizing the metal-ligand complex on a solid support; bringing a sample containing the nucleic acid into contact with the immobilized complex on the solid support to bind the nucleic acid to the complex; and adding a solution containing a chelate capable of removing the metal to elute the nucleic acid bound to the complex. The nucleic acids can be efficiently purified using a metal-ligand complex interacting with a base of the nucleic acid instead of interacting with a phosphoric acid of the nucleic acid to selectively bind the nucleic acid, and using a chelator capable of removing the metal to elute the nucleic acid.

    摘要翻译： 提供了使用金属 - 配体络合物纯化核酸的方法，所述方法包括：将所述金属 - 配体复合物固定在固体支持物上; 使含有核酸的样品与固体支持物上的固定化复合物接触以将核酸结合到复合物上; 并加入含有能除去金属的螯合物的溶液以洗脱与络合物结合的核酸。 可以使用与核酸的碱基相互作用的金属 - 配体复合物来有效纯化核酸，而不是与核酸的磷酸相互作用以选择性地结合核酸，并且使用能够去除金属洗脱的螯合剂 核酸。

    Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser
    6.
    发明授权
    Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权
    使用微磁珠和激光快速破坏细胞或病毒的方法和装置

    公开(公告)号：US07855069B2

    公开(公告)日：2010-12-21

    申请号：US11253541

    申请日：2005-10-19

    申请人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

    发明人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

    IPC分类号： C12M1/42 ,  C12M1/33

    CPC分类号： C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

    摘要： A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

    摘要翻译： 提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。 根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在 细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠将振动有效地转移到细胞中，通过疏水处理芯片的内表面和细胞来解决粗糙表面上的微流体问题 裂解芯片可应用于LOC。

    Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer
    8.
    发明授权
    Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
    使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法

    公开(公告)号：US07803539B2

    公开(公告)日：2010-09-28

    申请号：US11297783

    申请日：2005-12-07

    申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

    发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C07H21/00

    CPC分类号： C07H21/04

    摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.

    摘要翻译： 提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。 该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。 因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。 此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

    Hybridization chamber agitation device using pump and valves
    9.
    发明授权
    Hybridization chamber agitation device using pump and valves 失效
    使用泵和阀的杂化室搅拌装置

    公开(公告)号：US07544505B2

    公开(公告)日：2009-06-09

    申请号：US11295844

    申请日：2005-12-06

    申请人： Sung-ouk Jung ,  Nam Huh ,  Soo-suk Lee ,  Chang-eun Yoo ,  Hun-joo Lee

    发明人： Sung-ouk Jung ,  Nam Huh ,  Soo-suk Lee ,  Chang-eun Yoo ,  Hun-joo Lee

    IPC分类号： C12M1/34

    CPC分类号： B01L3/502738 ,  B01F13/0059 ,  B01F13/0222 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877

    摘要： Provided is an agitation device used to agitate a solution in a hybridization chamber, the agitation device including: the hybridization chamber; first and second air channels connected to ends of the hybridization chamber; a first valve disposed in the first air channel; a second valve disposed in the second air channel; an integrated air channel connecting the first and second air channels; and a pump disposed in the integrated air channel. The agitation device is suitable for effective diffusion of a sample when performing hybridization using a DNA chip. Therefore, a probe can be effectively hybridized with a target material.

    摘要翻译： 提供了用于搅拌杂交室中的溶液的搅拌装置，搅拌装置包括：杂交室; 第一和第二空气通道连接到杂交室的末端; 设置在第一空气通道中的第一阀; 设置在第二空气通道中的第二阀; 连接第一和第二空气通道的集成空气通道; 以及设置在集成空气通道中的泵。 搅拌装置适用于使用DNA芯片进行杂交时样品的有效扩散。 因此，探针可以有效地与靶材料杂交。