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    Nucleic acid purification method using hydrogen bonding and electric field
    2.
    发明申请
    Nucleic acid purification method using hydrogen bonding and electric field 审中-公开
    使用氢键和电场的核酸纯化方法

    公开(公告)号：US20060118417A1

    公开(公告)日：2006-06-08

    申请号：US11281226

    申请日：2005-11-16

    Applicant: Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

    Inventor： Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

    IPC: C07H21/04

    CPC classification number: C07H21/04

    Abstract: Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.

    Abstract translation: 提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。 根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。

    Apparatus for and method of purifying nucleic acids by different laser absorption of beads
    3.
    发明申请
    Apparatus for and method of purifying nucleic acids by different laser absorption of beads 审中-公开
    通过不同激光吸收珠粒来纯化核酸的装置和方法

    公开(公告)号：US20060110725A1

    公开(公告)日：2006-05-25

    申请号：US11190169

    申请日：2005-07-26

    Applicant: Jeong-gun Lee ,  Young-nam Kwon ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Young-a Kim

    Inventor： Jeong-gun Lee ,  Young-nam Kwon ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Young-a Kim

    IPC: C12Q1/70 ,  C12Q1/68 ,  C12N7/02 ,  C12N1/08 ,  C12M1/34

    CPC classification number: B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487

    Abstract: An apparatus for and method of purifying nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples, magnetic beads, and a solid support are introduced; a vibrator attached to the capillary and mixing the samples, magnetic beads, and solid support in the capillary; a laser generator attached to the capillary and irradiating a laser beam onto the capillary; a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall; a waste chamber attached to the capillary and discharging a lysate; an elution buffer chamber attached to the capillary and eluting nucleic acids from the solid support having nucleic acids bound thereto; and a neutralization buffer chamber attached to the capillary and supplying a neutralization buffer for neutralizing the eluted nucleic acid solution. According to the apparatus and method, PCR inhibitors can be removed to increase PCR yield and nucleic acids can be purified using a silicon substrate or silica beads. Thus, the apparatus and method can be applied to LOC fabrication.

    Abstract translation: 提供了一种纯化细胞或病毒核酸的装置和方法。 核酸纯化装置包括：细胞裂解毛细管，其具有样品入口，通过所述样品入口引入样品，磁珠和固体支持物; 连接到毛细管并将样品，磁珠和固体支持物混合在毛细管中的振动器; 激光发生器附接到毛细管并将激光束照射到毛细管上; 附着到毛细管并将磁珠固定到毛细管壁的磁力发生器; 连接到毛细管并排出裂解物的废物室; 洗脱缓冲室连接到毛细管并从具有与其结合的核酸的固体支持物洗脱核酸; 以及中和缓冲室，其连接到毛细管并提供用于中和洗脱的核酸溶液的中和缓冲液。 根据该装置和方法，可以除去PCR抑制剂以增加PCR产量，并且可以使用硅底物或二氧化硅珠纯化核酸。 因此，该装置和方法可以应用于LOC制造。

    MICROARRAY REACTION DEVICE AND METHOD OF USING THE SAME
    5.
    发明申请
    MICROARRAY REACTION DEVICE AND METHOD OF USING THE SAME 失效
    微波反应装置及其使用方法

    公开(公告)号：US20110190147A1

    公开(公告)日：2011-08-04

    申请号：US13007750

    申请日：2011-01-17

    Applicant: Won-seok CHUNG ,  Myo-yong LEE ,  Kak NAMKOONG ,  Woochang LEE

    Inventor： Won-seok CHUNG ,  Myo-yong LEE ,  Kak NAMKOONG ,  Woochang LEE

    IPC: C40B30/00 ,  C40B60/12 ,  C40B40/06 ,  C40B40/10

    CPC classification number: C40B30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/12

    Abstract: A microarray reaction device includes a fluid container, a reaction chamber, a first channel connected with the fluid container, a second channel connected with the reaction chamber, and a valve. The valve includes a first and second support unit, respectively including a first and second penetration opening unit, extended through a first and second surface thereof. The first and second penetration opening unit is connected to a second end of the first and second channel, respectively. The second support unit includes a third penetration opening unit extended through a second surface thereof. The first and second surfaces contact each other, such that the first support unit and the second support unit are slidably disposed with each other. The microarray reaction device further includes a storing chamber connected with the third penetration opening unit, and a pump connected to the storing chamber and providing pressure to the storing chamber.

    Abstract translation: 微阵列反应装置包括流体容器，反应室，与流体容器连接的第一通道，与反应室连接的第二通道和阀。 阀包括第一和第二支撑单元，分别包括延伸穿过其第一和第二表面的第一和第二穿透开口单元。 第一和第二穿透开口单元分别连接到第一和第二通道的第二端。 第二支撑单元包括延伸穿过其第二表面的第三穿透开口单元。 第一和第二表面彼此接触，使得第一支撑单元和第二支撑单元彼此可滑动地布置。 微阵列反应装置还包括与第三穿透开口单元连接的储存室，以及连接到储存室并向储存室提供压力的泵。

    Method of purifying RNA using kosmotropic salt
    6.
    发明授权
    Method of purifying RNA using kosmotropic salt 有权
    使用去离子水纯化RNA的方法

    公开(公告)号：US07923551B2

    公开(公告)日：2011-04-12

    申请号：US12110391

    申请日：2008-04-28

    Applicant: Myo-yong Lee ,  Nam Huh ,  Joon-ho Kim

    Inventor： Myo-yong Lee ,  Nam Huh ,  Joon-ho Kim

    IPC: C07H21/00

    CPC classification number: C07H21/02 ,  C12N15/1003 ,  C12N15/1006

    Abstract: The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.

    Abstract translation: 本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。 根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。 本发明在纯化200个或更少核苷酸的RNA中特别有效。

    Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser
    7.
    发明授权
    Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权
    使用微磁珠和激光快速破坏细胞或病毒的方法和装置

    公开(公告)号：US07855069B2

    公开(公告)日：2010-12-21

    申请号：US11253541

    申请日：2005-10-19

    Applicant: Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

    Inventor： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

    IPC: C12M1/42 ,  C12M1/33

    CPC classification number: C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

    Abstract: A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

    Abstract translation: 提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。 根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在 细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠将振动有效地转移到细胞中，通过疏水处理芯片的内表面和细胞来解决粗糙表面上的微流体问题 裂解芯片可应用于LOC。

    METHOD AND APPARATUS FOR NUCLEIC PURIFICATION USING ION-PERMEABLE POLYMER-COATED ELECTRODE
    9.
    发明申请
    METHOD AND APPARATUS FOR NUCLEIC PURIFICATION USING ION-PERMEABLE POLYMER-COATED ELECTRODE 审中-公开
    使用透明聚合物涂覆电极进行核纯化的方法和装置

    公开(公告)号：US20070073049A1

    公开(公告)日：2007-03-29

    申请号：US11534896

    申请日：2006-09-25

    Applicant: Myo-Yong Lee ,  Jung Lee

    Inventor： Myo-Yong Lee ,  Jung Lee

    IPC: C07H21/04

    CPC classification number: C07H21/04 ,  C12N15/1006 ,  C12N15/101

    Abstract: A method and apparatus for nucleic acid purification using an ion-permeable polymer-coated electrode are provided. The method for nucleic acid purification according to the present invention comprises the steps of exposing a fluid sample containing nucleic acids to an electrode coated with an ion-permeable polymer, applying a voltage to the electrode to attach the nucleic acids in the fluid sample thereto, removing the residual fluid sample from the electrode carrying the nucleic acids, and eluting the nucleic acids attached to the electrode into a buffer. The apparatus comprises an electrode coated with an ion-permeable polymer; and a voltage applying device for applying a voltage to the electrode.

    Abstract translation: 提供了使用离子透过性聚合物涂覆电极进行核酸纯化的方法和装置。 根据本发明的用于核酸纯化的方法包括以下步骤：将含有核酸的流体样品暴露于涂覆有离子可渗透聚合物的电极，向电极施加电压以将核酸附着在流体样品中， 从携带核酸的电极中除去残余流体样品，并将附着于电极的核酸洗脱到缓冲液中。 该装置包括涂覆有离子可渗透聚合物的电极; 以及用于向电极施加电压的电压施加装置。

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