公开(公告)号：US20180161775A1

公开(公告)日：2018-06-14

申请号：US15891579

申请日：2018-02-08

Applicant: The General Hospital Corporation

Inventor： Ravi Kapur ,  Kyle C. Smith ,  Mehmet Toner

IPC: B01L3/00 ,  G01N15/14 ,  A61K35/28 ,  G01N1/40 ,  G01N15/02 ,  G01N15/06 ,  G01N15/00

CPC classification number: B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1493

Abstract: A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

公开(公告)号：US20180161774A1

公开(公告)日：2018-06-14

申请号：US15579684

申请日：2016-06-17

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino DI CARLO ,  Manjima DHAR

IPC: B01L3/00 ,  G01N1/34 ,  G01N15/14

CPC classification number: B01L3/502761 ,  A61K35/13 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0487 ,  G01N1/34 ,  G01N15/1484 ,  G01N2015/1062 ,  G01N2015/1081

Abstract: A microfluidic device for trapping circulating tumor cells includes at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel having a height of less than 50 μm and a width less than 30 μm. A plurality of expansion regions are disposed along the length of the at least one microfluidic channel, each of the plurality of expansion regions is formed by an abrupt increase in the width of the at least one microfluidic channel, wherein the width of each expansion region is within the range of 526 μm to 626 μm and continues for a length within the range of 814 μm to 914 μm along a length of the expansion region, followed by an abrupt decrease in the width of the at least one microfluidic channel back to a width less than 30 μm.

公开(公告)号：US20180154361A1

公开(公告)日：2018-06-07

申请号：US15873894

申请日：2018-01-17

Applicant: Owl biomedical, Inc.

Inventor： John S. FOSTER ,  Kevin E. SHIELDS ,  Mehran R. Hoonejani ,  Adam G. Swanson

IPC: B01L3/00 ,  F16K99/00 ,  G01N15/14 ,  G01N21/64

CPC classification number: B01L3/502761 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2400/0622 ,  B01L2400/0633 ,  F16K99/0046 ,  G01N15/1056 ,  G01N15/1404 ,  G01N15/1484 ,  G01N21/6402 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149 ,  G01N2021/6439 ,  G01N2201/06113

Abstract: A particle manipulation system uses a MEMS-based, microfabricated particle manipulation device which has a sample inlet channel, output channels, and a movable member formed on a substrate. The device may be used to separate a target particle from non-target material in a sample stream. In order to improve the sorter speed, accuracy or yield, the particle manipulation system may also include a microfluidic structure which focuses the target particles in a particular portion of the sample inlet channel. The device may be manufactured using three or more substrates in a wafer stack, and each device may be singulated from the wafer stack using submerged trenches in the middle substrate.

公开(公告)号：US09981267B2

公开(公告)日：2018-05-29

申请号：US15183531

申请日：2016-06-15

Applicant: The Government of the United States of America, as represented by the Secretary of the Navy

Inventor： Sean J. Hart ,  Sarah J. R. Staton ,  Alexander V. Terray ,  Gregory E. Collins

IPC: B01L3/00 ,  G01N1/10 ,  B03C5/02 ,  B81B7/02 ,  G01N15/10

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2300/0864 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0454 ,  B01L2400/0487 ,  B81B7/02 ,  G01N2015/1081

Abstract: The combined value of integrating optical forces and electrokinetics allows for the pooled separation vectors of each to be applied, providing for separation based on combinations of features such as size, shape, refractive index, charge, charge distribution, charge mobility, permittivity, and deformability. The interplay of these separation vectors allow for the selective manipulation of analytes with a finer degree of variation. Embodiments include methods of method of separating particles in a microfluidic channel using a device comprising a microfluidic channel, a source of laser light focused by an optic into the microfluidic channel, and a source of electrical field operationally connected to the microfluidic channel via electrodes so that the laser light and the electrical field to act jointly on the particles in the microfluidic channel. Other devices and methods are disclosed.

公开(公告)号：US20180133715A1

公开(公告)日：2018-05-17

申请号：US15578455

申请日：2016-06-03

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Frank F. CRAIG ,  Marian REHAK ,  David HOLMES ,  Clive A. SMITH ,  Xin LIU ,  Giuseppe BENAZZI ,  Xin LI ,  Vinayaka PAWATE

IPC: B01L3/00 ,  G01N35/08 ,  G01N15/14

CPC classification number: B01L3/502784 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0627 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/0644 ,  G01N15/1484 ,  G01N35/08 ,  G01N2015/1481 ,  G01N2015/149

Abstract: Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.

公开(公告)号：US20180117585A1

公开(公告)日：2018-05-03

申请号：US15829371

申请日：2017-12-01

Applicant: President and Fellows of Harvard College

Inventor： David A. Weitz ,  Darren Roy Link ,  Galder Cristobal-Azkarate ,  Zhengdong Cheng ,  Keunho Ahn

IPC: B01L3/00 ,  B01J19/00 ,  G01N15/14 ,  G01N15/10 ,  B01F5/06 ,  B01F13/00 ,  B01L3/02 ,  B01F5/02

CPC classification number: B01L3/5027 ,  B01F5/0256 ,  B01F5/0646 ,  B01F5/0655 ,  B01F5/0682 ,  B01F5/0689 ,  B01F13/0069 ,  B01F13/0071 ,  B01F13/0074 ,  B01F13/0076 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00862 ,  B01L3/0241 ,  B01L3/502761 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/5088 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/161 ,  B01L2400/0415 ,  B01L2400/0439 ,  B01L2400/0487 ,  C12Q2563/159 ,  C12Q2565/629 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1459 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149 ,  Y10S436/807 ,  Y10T436/118339 ,  Y10T436/2525 ,  Y10T436/2575

Abstract: Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. In some cases, the droplets may each have a substantially uniform number of entities therein. For example, 95% or more of the droplets may each contain the same number of entities of a particular species. In another aspect, the invention relates to systems and methods for dividing a fluidic droplet into two droplets, for example, through charge and/or dipole interactions with an electric field. The invention also relates to systems and methods for fusing droplets according to another aspect of the invention, for example, through charge and/or dipole interactions. In some cases, the fusion of the droplets may initiate or determine a reaction. In a related aspect of the invention, systems and methods for allowing fluid mixing within droplets to occur are also provided. In still another aspect, the invention relates to systems and methods for sorting droplets, e.g., by causing droplets to move to certain regions within a fluidic system. Examples include using electrical interactions (e.g., charges, dipoles, etc.) or mechanical systems (e.g., fluid displacement) to sort the droplets. In some cases, the fluidic droplets can be sorted at relatively high rates, e.g., at about 10 droplets per second or more. Another aspect of the invention provides the ability to determine droplets, or a component thereof, for example, using fluorescence and/or other optical techniques (e.g., microscopy), or electric sensing techniques such as dielectric sensing.

公开(公告)号：US20180113130A1

公开(公告)日：2018-04-26

申请号：US15812105

申请日：2017-11-14

Applicant: The Trustees of the University of Pennsylvania

Inventor： Stephen M. Hahn ,  Jay F. Dorsey ,  Gary D. Kao ,  Emigdio Reyes

IPC: G01N33/574 ,  B01L3/00 ,  C12Q1/6897 ,  G01N33/543

CPC classification number: G01N33/574 ,  B01L3/502753 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/086 ,  C12Q1/6897 ,  G01N33/54366 ,  G01N2030/524 ,  G01N30/6095 ,  G01N30/6069

Abstract: A repeatable method for detecting circulating tumor cells in vitro is provided. The method involves combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and culture media for the cells. The adenoviral system utilizes (i) a first replication-defective adenoviral particle in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5′ and 3′ ITRs and a tumor-specific promoter; and (ii) a coding sequence for a reporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3′ ITR. The test sample and the non-lytic adenoviral system are incubated for a sufficient time to permit expression of the reporter protein, and measuring reporter protein expression in the test samples, whereby presence of reporter expression indicates the presence of circulating tumor cells in the sample. Because the system is non-lytic, the testing can be repeated on the cells which remain viable in culture. Also provided is a method for enriching test samples having circulating tumor cells and a microfluidics device suitable for CTC-specification identification and enumeration.

公开(公告)号：US20180106805A1

公开(公告)日：2018-04-19

申请号：US15565122

申请日：2016-04-05

Applicant: TumorGen MDx LLC

Inventor： Jeffrey K. Allen ,  Alexander G. Allen ,  Austin E. Allen

IPC: G01N33/574 ,  B01L3/00 ,  G01N33/543 ,  C12Q1/6869 ,  G01N33/548 ,  G01N33/58

CPC classification number: G01N33/57407 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0677 ,  B01L2400/086 ,  C12M47/04 ,  C12Q1/68 ,  C12Q1/6869 ,  G01N27/44791 ,  G01N33/54386 ,  G01N33/548 ,  G01N33/57492 ,  G01N33/582 ,  G01N2030/8813 ,  G01N2800/52

Abstract: This invention concerns patentable devices configured to capture cancer stem cells and cell clusters. After capturing such cells and/or clusters, the cancerous cells are subjected to whole genome sequencing. The resulting genomic sequence information is then compared to that for “normal” or non-diseased tissue (obtained, for example, from either the same patient, or a population sample, etc.) in order to identify the specific genetic mutation(s) present in the CSCs. Further analysis then correlates the genetic mutations with cell growth signaling pathways typically found with tumor metastases. Armed with this information, an oncologist can then develop a specifically targeted therapy that utilizes approved drugs or drug candidates undergoing clinical testing to address the identified driver mutations and thus effect a “targeted” therapy tailored to the particular patient's disease.

公开(公告)号：US20180093271A1

公开(公告)日：2018-04-05

申请号：US15720515

申请日：2017-09-29

Applicant: The University of Tokyo ,  ARKRAY, Inc.

Inventor： Teruo Fujii ,  Soo Hyeon Kim ,  Hiroshi Ito

IPC: B01L3/00 ,  G01N1/40 ,  G01N15/14 ,  B03C5/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/08 ,  B01L2300/0816 ,  B01L2400/0421 ,  B01L2400/0424 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N1/40 ,  G01N15/1484 ,  G01N2001/4038 ,  G01N2015/0065 ,  G01N2015/1006

Abstract: The present disclosure relates to a microdevice for capturing particles from a sample, a method for capturing particles from a sample, and a method for concentrating or separating particles using the same.

公开(公告)号：US20180080060A1

公开(公告)日：2018-03-22

申请号：US15270306

申请日：2016-09-20

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Stacey M. Gifford ,  Joshua T. Smith ,  Benjamin H. Wunsch

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/6806 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/143 ,  B01L2300/0816 ,  B01L2300/0896 ,  B01L2400/086 ,  G01N15/02 ,  G01N15/1056 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/0288 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/1087 ,  G01N2015/149 ,  C12Q2565/629

Abstract: A technique relates to separation of a mixture. A nano-deterministic lateral displacement (nanoDLD) array is configured to separate the mixture in a fluid. A feedback system is configured to control a velocity of the fluid through the nanoDLD array. The feedback system is configured to control the velocity of the fluid to separate one or more entities in the mixture.