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    SEQUENCE-SPECIFIC DETECTION AND PHENOTYPE DETERMINATION
    72.
    发明申请
    SEQUENCE-SPECIFIC DETECTION AND PHENOTYPE DETERMINATION 审中-公开
    序列特异性检测和基因定位

    公开(公告)号：US20160319378A1

    公开(公告)日：2016-11-03

    申请号：US15136646

    申请日：2016-04-22

    申请人： GeneWeave Biosciences, Inc.

    发明人： Diego Ariel Rey

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6897 ,  C12Q1/689

    摘要： Disclosed herein are materials and methods for achieving sequence-specific organism detection and/or phenotype(s) using sequence-specific oligonucleotides. Also disclosed are related kits, cultures, and cells for detecting and/or phenotyping microorganisms in a sequence-specific manner.

    摘要翻译： 本文公开了使用序列特异性寡核苷酸获得序列特异性生物体检测和/或表型的材料和方法。 还公开了用于以序列特异性方式检测和/或表型微生物的相关试剂盒，培养物和细胞。

    Methods for genomic integration
    75.
    发明授权
    Methods for genomic integration 有权
    基因组整合方法

    公开(公告)号：US09476065B2

    公开(公告)日：2016-10-25

    申请号：US14577997

    申请日：2014-12-19

    申请人： Amyris, Inc.

    发明人： Andrew Horwitz ,  Kristy Michelle Hawkins ,  Max Schubert ,  Wayne Szeto

    IPC分类号： C12N9/22 ,  C12N15/90 ,  C12Q1/68 ,  C12N15/11

    CPC分类号： C12N15/907 ,  C12N15/111 ,  C12N15/902 ,  C12N2310/20 ,  C12Q1/6897

    摘要： Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.

    摘要翻译： 本文提供将一种或多种外源核酸整合到宿主细胞基因组的一个或多个选定靶位点的方法。 在某些实施方案中，所述方法包括使宿主细胞基因组与包含待整合到基因组靶位点的外源核酸的一个或多个整合多核苷酸，能够在基因组靶位点引起断裂的核酸酶和线性核酸 能够与其自身或与一个或多个与细胞群接触的另外的线性核酸进行同源重组，于是所述同源重组导致形成包含可选择标记的编码序列的环状染色体外核酸。 在一些实施方案中，所述方法还包括选择表达可选择标记的宿主细胞。

    METHOD FOR PREVENTING AND TREATING RENAL DISEASE
    78.
    发明申请
    METHOD FOR PREVENTING AND TREATING RENAL DISEASE 审中-公开
    预防和治疗肾病的方​​法

    公开(公告)号：US20160220600A1

    公开(公告)日：2016-08-04

    申请号：US14967831

    申请日：2015-12-14

    申请人： L & F Research LLC

    发明人： Alessia Fornoni ,  Sandra Merscher-Gomez

    IPC分类号： A61K31/724 ,  A61K45/06

    CPC分类号： A61K31/724 ,  A61K38/1774 ,  A61K39/3955 ,  A61K45/06 ,  C12Q1/6897 ,  G01N33/5044 ,  G01N33/5091 ,  G01N33/56966 ,  G01N2800/245 ,  G01N2800/347 ,  G01N2800/50

    摘要： Assays, methods and kits for predicting a subject's (e.g., human) risk of primary glomerulopathy, secondary glomerulopathy or recurrence (e.g., post-transplant recurrence) of any glomerular disease include examining cells for the presence or absence of cytoskeletal disruptions or rearrangements and examining cells for modulation of expression and/or activity of markers such as SMPDL-3b. Assays for predicting if a diabetic subject will develop kidney disease or a patient with FSGS will develop recurrent disease after transplant also include examining cells for the presence or absence of cytoskeletal disruptions or rearrangements and examining cells for modulation of expression and/or activity of markers such as SMPDL-3b. Also described herein are compositions and methods for treating and preventing the aforementioned disorders.

    摘要翻译： 用于预测任何肾小球疾病的原发性肾小球病，继发性肾小球病或继发性肾小球病或复发（例如移植后复发）的受试者（例如，人）风险的测定，方法和试剂盒包括检查细胞是否存在细胞骨架破坏或重排以及检查 调节SMPDL-3b等标记物的表达和/或活性的细胞。 用于预测糖尿病患者是否发展为肾脏疾病或FSGS患者的测定将在移植后发展复发性疾病，还包括检查细胞是否存在细胞骨架破坏或重排，并检查细胞以调节标志物的表达和/或活性，如 作为SMPDL-3b。 本文还描述了用于治疗和预防上述疾病的组合物和方法。

    HIGH THROUGHPUT NUCLEIC ACID SEQUENCING BY EXPANSION
    79.
    发明申请
    HIGH THROUGHPUT NUCLEIC ACID SEQUENCING BY EXPANSION 审中-公开

    公开(公告)号：US20160208344A1

    公开(公告)日：2016-07-21

    申请号：US14792402

    申请日：2015-07-06

    申请人： Stratos Genomics

    发明人： Mark Stamatios Kokoris ,  Robert N. McRuer

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6897 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2565/631 ,  C12Q2525/197 ,  C12Q2525/131

    摘要： Nucleic acid sequencing methods and related products are disclosed. Methods for sequencing a target nucleic acid comprise providing a daughter strand produced by a template-directed synthesis, the daughter strand comprising a plurality of subunits coupled in a sequence corresponding to a contiguous nucleotide sequence of all or a portion of the target nucleic acid, wherein the individual subunits comprise a tether, at least one probe or nucleobase residue, and at least one selectively cleavable bond. The selectively cleavable bond(s) is/are cleaved to yield an Xpandomer of a length longer than the plurality of the subunits of the daughter strand, the Xpandomer comprising the tethers and reporter elements for parsing genetic information in a sequence corresponding to the contiguous nucleotide sequence of all or a portion of the target nucleic acid. Reporter elements of the Xpandomer are then detected. Corresponding products, including Xpandomers and oligomeric and monomeric substrate constructs are also disclosed.

    NOVEL REPORTER CONSTRUCTS FOR COMPOUND SCREENING
    80.
    发明申请
    NOVEL REPORTER CONSTRUCTS FOR COMPOUND SCREENING 审中-公开
    用于复合筛选的新型报告器结构

    公开(公告)号：US20160201146A1

    公开(公告)日：2016-07-14

    申请号：US15084800

    申请日：2016-03-30

    申请人： Joshua I. Armstrong ,  Hans E. Holtan ,  Masa-aki Ohto ,  Adina M. Bailey

    发明人： Joshua I. Armstrong ,  Hans E. Holtan ,  Masa-aki Ohto ,  Adina M. Bailey

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6897 ,  C12N15/8237 ,  C12N15/8261 ,  C12N15/8273 ,  C12Q1/6876

    摘要： The instant description provides reporter constructs, transgenic cells, and transgenic organisms and methods for identifying agents that can regulate gene expression and improve plant performance and yield. Compounds that increase plant performance or yield are identified by contacting a test compound with a plant cell that comprises a target promoter sequence operably linked to a polynucleotide sequence encoding a DNA sequence-specific transactivator, and a reporter polynucleotide that is operably linked to a promoter sequence that is recognized by the DNA sequence-specific transactivator. The target promoter sequence can be recognized by a transcriptional regulatory polypeptide capable of modulating specific signaling pathways that enhance plant performance or yield.

    摘要翻译： 即时描述提供了报道构建体，转基因细胞和转基因生物以及用于鉴定可调节基因表达并改善植物性能和产量的试剂的方法。 通过使测试化合物与包含与编码DNA序列特异性反式激活因子的多核苷酸序列可操作地连接的靶启动子序列的植物细胞和与启动子序列可操作地连接的报告多核苷酸来鉴定提高植物性能或产量的化合物 这被DNA序列特异性反式激活因子识别。 靶启动子序列可以通过能够调节提高植物表现或产量的特异性信号通路的转录调节多肽来识别。