NOVEL REPORTER CONSTRUCTS FOR COMPOUND SCREENING
    5.
    发明申请
    NOVEL REPORTER CONSTRUCTS FOR COMPOUND SCREENING 审中-公开
    用于复合筛选的新型报告器结构

    公开(公告)号:US20160201146A1

    公开(公告)日:2016-07-14

    申请号:US15084800

    申请日:2016-03-30

    IPC分类号: C12Q1/68

    摘要: The instant description provides reporter constructs, transgenic cells, and transgenic organisms and methods for identifying agents that can regulate gene expression and improve plant performance and yield. Compounds that increase plant performance or yield are identified by contacting a test compound with a plant cell that comprises a target promoter sequence operably linked to a polynucleotide sequence encoding a DNA sequence-specific transactivator, and a reporter polynucleotide that is operably linked to a promoter sequence that is recognized by the DNA sequence-specific transactivator. The target promoter sequence can be recognized by a transcriptional regulatory polypeptide capable of modulating specific signaling pathways that enhance plant performance or yield.

    摘要翻译: 即时描述提供了报道构建体,转基因细胞和转基因生物以及用于鉴定可调节基因表达并改善植物性能和产量的试剂的方法。 通过使测试化合物与包含与编码DNA序列特异性反式激活因子的多核苷酸序列可操作地连接的靶启动子序列的植物细胞和与启动子序列可操作地连接的报告多核苷酸来鉴定提高植物性能或产量的化合物 这被DNA序列特异性反式激活因子识别。 靶启动子序列可以通过能够调节提高植物表现或产量的特异性信号通路的转录调节多肽来识别。

    Reporter constructs for compound screening
    6.
    发明授权
    Reporter constructs for compound screening 有权
    记者构建复合筛查

    公开(公告)号:US09322070B2

    公开(公告)日:2016-04-26

    申请号:US13699527

    申请日:2011-05-23

    IPC分类号: C12Q1/68 C12N15/82 C12N15/79

    摘要: The instant description provides reporter constructs, transgenic cells, and transgenic organisms and methods for identifying agents that can regulate gene expression and improve plant performance and yield. Compounds that increase plant performance or yield are identified by contacting a test compound with a plant cell that comprises a target promoter sequence operably linked to a polynucleotide sequence encoding a DNA sequence-specific transactivator, and a reporter polynucleotide that is operably linked to a promoter sequence that is recognized by the DNA sequence-specific transactivator. The target promoter sequence can be recognized by a transcriptional regulatory polypeptide capable of modulating specific signaling pathways that enhance plant performance or yield.

    摘要翻译: 即时描述提供了报道构建体,转基因细胞和转基因生物以及用于鉴定可调节基因表达并改善植物性能和产量的试剂的方法。 通过使测试化合物与包含与编码DNA序列特异性反式激活因子的多核苷酸序列可操作地连接的靶启动子序列的植物细胞和与启动子序列可操作地连接的报告多核苷酸来鉴定提高植物性能或产量的化合物 这被DNA序列特异性反式激活因子识别。 靶启动子序列可以通过能够调节提高植物表现或产量的特异性信号通路的转录调节多肽来识别。