公开(公告)号：US20170233783A1

公开(公告)日：2017-08-17

申请号：US15365271

申请日：2016-11-30

申请人： GeneWeave Biosciences, Inc.

发明人： Nikol DE FOREST ,  Werner FREI ,  Diego Rey ,  Shaunak Roy ,  Soni SHUKLA ,  Ryan C. GRISWOLD ,  Kenneth G. OLSON ,  Bruce J. RICHARDSON ,  Victor H. Yee

IPC分类号： C12Q1/04 ,  C12Q1/68 ,  B01L3/00

CPC分类号： C12Q1/04 ,  B01L3/502 ,  B01L3/50825 ,  B01L3/523 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2300/0867 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0683 ,  C12Q1/6897 ,  G01N21/6486 ,  G01N21/76 ,  G01N21/77 ,  G01N2021/0325 ,  G01N2021/0328 ,  G01N2021/6443 ,  G01N2021/7786

摘要： An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

公开(公告)号：US09133497B2

公开(公告)日：2015-09-15

申请号：US14611902

申请日：2015-02-02

申请人： GeneWeave Biosciences, Inc.

发明人： Werner Frei ,  Diego Ariel Rey ,  Shaunak Roy ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Rick V. Stellmacher

IPC分类号： G01N21/13 ,  G01N21/15 ,  G01N21/17 ,  C12Q1/04 ,  B01L3/00 ,  G01N21/01 ,  G01N21/76 ,  G01N21/03 ,  C12Q1/68 ,  B01L7/00 ,  C12Q1/02 ,  G01J1/00 ,  C12Q1/66 ,  G01N35/00 ,  G01N35/04

CPC分类号： C12Q1/6897 ,  B01L3/502 ,  B01L3/50825 ,  B01L3/52 ,  B01L3/523 ,  B01L3/54 ,  B01L3/545 ,  B01L7/00 ,  B01L2200/028 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2400/0478 ,  B01L2400/06 ,  B01L2400/0683 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/66 ,  G01J1/00 ,  G01N21/01 ,  G01N21/03 ,  G01N21/13 ,  G01N21/76 ,  G01N21/763 ,  G01N2035/00346 ,  G01N2035/0405 ,  G01N2333/195

摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

摘要翻译： 本文描述了使用工程化转导颗粒检测和/或鉴定靶细胞（例如细菌）的系统和方法。 在一些实施方案中，一种方法包括将一定量的转导颗粒混合在样品内。 转导颗粒与靶细胞相关联。 转导颗粒是非复制型的，并被工程化以包括配制成使靶细胞产生一系列报道分子的核酸分子。 当靶细胞存在于样品中时，保持样品和转导颗粒以表达一系列报告分子。 接收与一定量的报道分子相关的信号。 在一些实施例中，信号的幅度与高于预定量的转导颗粒的数量无关。

公开(公告)号：US10240212B2

公开(公告)日：2019-03-26

申请号：US15388750

申请日：2016-12-22

申请人： GeneWeave Biosciences, Inc.

发明人： Diego Ariel Rey ,  Shaunak Roy ,  Leonardo Maestri Teixeira ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Victor H. Yee ,  Werner Frei

IPC分类号： C12Q1/06 ,  C12Q1/10 ,  C12Q1/66 ,  C12Q1/68 ,  C12Q1/6897 ,  C12Q1/04 ,  B01L3/00 ,  G01N21/01 ,  G01N21/76 ,  G01N21/03 ,  G01N21/13 ,  B01L7/00 ,  C12Q1/02 ,  G01J1/00 ,  C12Q1/14 ,  G01N35/00 ,  G01N35/04

摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

公开(公告)号：US10125386B2

公开(公告)日：2018-11-13

申请号：US15365271

申请日：2016-11-30

申请人： GeneWeave Biosciences, Inc.

发明人： Nikol De Forest ,  Werner Frei ,  Diego Rey ,  Shaunak Roy ,  Soni Shukla ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Victor H. Yee

IPC分类号： C12Q1/04 ,  C12Q1/68 ,  B01L3/00 ,  G01N21/64 ,  G01N21/76 ,  C12Q1/6897 ,  G01N21/03 ,  G01N21/77

摘要： An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

公开(公告)号：US09546391B2

公开(公告)日：2017-01-17

申请号：US14480269

申请日：2014-09-08

申请人： GeneWeave Biosciences, Inc.

发明人： Diego Ariel Rey ,  Shaunak Roy ,  Werner Frei ,  Bruce J. Richardson ,  Ryan C. Griswold ,  Damian S. Matthews ,  Kenneth G. Olson ,  Victor H. Yee

IPC分类号： C12M1/00 ,  B01L3/00 ,  C12Q1/04 ,  G01N21/01 ,  G01N21/76 ,  G01N21/03 ,  G01N21/13 ,  C12Q1/68 ,  B01L7/00 ,  C12Q1/02 ,  G01J1/00 ,  C12Q1/66 ,  G01N35/00 ,  G01N35/04

CPC分类号： C12Q1/6897 ,  B01L3/502 ,  B01L3/50825 ,  B01L3/52 ,  B01L3/523 ,  B01L3/54 ,  B01L3/545 ,  B01L7/00 ,  B01L2200/028 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2400/0478 ,  B01L2400/06 ,  B01L2400/0683 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/66 ,  G01J1/00 ,  G01N21/01 ,  G01N21/03 ,  G01N21/13 ,  G01N21/76 ,  G01N21/763 ,  G01N2035/00346 ,  G01N2035/0405 ,  G01N2333/195

摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

摘要翻译： 本文描述了使用工程化转导颗粒检测和/或鉴定靶细胞（例如细菌）的系统和方法。 在一些实施方案中，一种方法包括将一定量的转导颗粒混合在样品内。 转导颗粒与靶细胞相关联。 转导颗粒是非复制型的，并被工程化以包括配制成使靶细胞产生一系列报道分子的核酸分子。 当靶细胞存在于样品中时，保持样品和转导颗粒以表达一系列报告分子。 接收与一定量的报道分子相关的信号。 在一些实施例中，信号的幅度与高于预定量的转导颗粒的数量无关。

公开(公告)号：US10351893B2

公开(公告)日：2019-07-16

申请号：US15283797

申请日：2016-10-03

申请人： GeneWeave Biosciences, Inc.

发明人： Doran Donnelly ,  Werner Frei ,  Ryan Griswold ,  Lance Page ,  Shaunak Roy

IPC分类号： B01L9/00 ,  C12Q1/04 ,  B01L3/00 ,  C12Q1/66

摘要： An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. A delivery portion of the housing defines a delivery path between the reagent volume and the reaction chamber when the housing is coupled to the reaction chamber. The delivery path includes a protrusion such that the delivery path has a discontinuous inner surface. The actuator can be moved to convey a reagent from the reagent container into the reaction chamber via the delivery path.

公开(公告)号：US09481903B2

公开(公告)日：2016-11-01

申请号：US13802461

申请日：2013-03-13

申请人： GeneWeave Biosciences, Inc.

发明人： Diego Ariel Rey ,  Shaunak Roy ,  Leonardo Maestri Teixeira ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Victor Yee

IPC分类号： C12Q1/70 ,  C12Q1/66 ,  C12Q1/02 ,  C12Q1/68 ,  G01N21/00 ,  C12Q1/04 ,  B01L3/00 ,  G01N21/01 ,  G01N21/76 ,  G01N21/03 ,  G01N21/13 ,  B01L7/00 ,  G01J1/00 ,  G01N35/00 ,  G01N35/04

CPC分类号： C12Q1/6897 ,  B01L3/502 ,  B01L3/50825 ,  B01L3/52 ,  B01L3/523 ,  B01L3/54 ,  B01L3/545 ,  B01L7/00 ,  B01L2200/028 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2400/0478 ,  B01L2400/06 ,  B01L2400/0683 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/66 ,  G01J1/00 ,  G01N21/01 ,  G01N21/03 ,  G01N21/13 ,  G01N21/76 ,  G01N21/763 ,  G01N2035/00346 ,  G01N2035/0405 ,  G01N2333/195

摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

公开(公告)号：US11149295B2

公开(公告)日：2021-10-19

申请号：US16454918

申请日：2019-06-27

申请人： GeneWeave Biosciences, Inc.

发明人： Doran Donnelly ,  Werner Frei ,  Ryan Griswold ,  Lance Page ,  Shaunak Roy

IPC分类号： B01L3/14 ,  C12Q1/04 ,  B01L3/00 ,  C12Q1/66

摘要： An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. A delivery portion of the housing defines a delivery path between the reagent volume and the reaction chamber when the housing is coupled to the reaction chamber. The delivery path includes a protrusion such that the delivery path has a discontinuous inner surface. The actuator can be moved to convey a reagent from the reagent container into the reaction chamber via the delivery path.

公开(公告)号：US09540675B2

公开(公告)日：2017-01-10

申请号：US14617631

申请日：2015-02-09

申请人： GeneWeave Biosciences, Inc.

发明人： Nikol De Forest ,  Werner Frei ,  Diego Rey ,  Shaunak Roy ,  Soni Shukla ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Victor H. Yee

IPC分类号： B01L3/00 ,  C12Q1/04 ,  C12Q1/68

CPC分类号： C12Q1/04 ,  B01L3/502 ,  B01L3/50825 ,  B01L3/523 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2300/0867 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0683 ,  C12Q1/6897 ,  G01N21/6486 ,  G01N21/76 ,  G01N21/77 ,  G01N2021/0325 ,  G01N2021/0328 ,  G01N2021/6443 ,  G01N2021/7786

摘要： An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

摘要翻译： 一种装置包括壳体和致动器。 限定可容纳试剂容器的试剂体积的外壳可以可拆卸地联接到反应室。 壳体包括定影与试剂体积流体连通的传送路径的穿刺器。 当壳体联接到反应室时，壳体的传送部分限定了传送路径和反应室之间的传送路径。 致动器具有设置在试剂体积内的柱塞部分。 可以操纵致动器的接合部分以使试剂体积内的柱塞部分移动以使试剂容器变形。 穿刺器可以刺穿试剂容器的易碎部分，以将试剂从试剂容器经由转移通路和/或输送通道输送到反应室中。

公开(公告)号：US20140272928A1

公开(公告)日：2014-09-18

申请号：US13802461

申请日：2013-03-13

申请人： GeneWeave Biosciences, Inc.

发明人： Diego Ariel REY ,  Shaunak Roy ,  Leonardo Maestri Teixeira ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Victor Yee

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6897 ,  B01L3/502 ,  B01L3/50825 ,  B01L3/52 ,  B01L3/523 ,  B01L3/54 ,  B01L3/545 ,  B01L7/00 ,  B01L2200/028 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2400/0478 ,  B01L2400/06 ,  B01L2400/0683 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/66 ,  G01J1/00 ,  G01N21/01 ,  G01N21/03 ,  G01N21/13 ,  G01N21/76 ,  G01N21/763 ,  G01N2035/00346 ,  G01N2035/0405 ,  G01N2333/195

摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

摘要翻译： 本文描述了使用工程化转导颗粒检测和/或鉴定靶细胞（例如细菌）的系统和方法。 在一些实施方案中，一种方法包括将一定量的转导颗粒混合在样品内。 转导颗粒与靶细胞相关联。 转导颗粒是非复制型的，并被工程化以包括配制成使靶细胞产生一系列报道分子的核酸分子。 当靶细胞存在于样品中时，保持样品和转导颗粒以表达一系列报告分子。 接收与一定量的报道分子相关的信号。 在一些实施例中，信号的幅度与高于预定量的转导颗粒的数量无关。