公开(公告)号：US10125386B2

公开(公告)日：2018-11-13

申请号：US15365271

申请日：2016-11-30

申请人： GeneWeave Biosciences, Inc.

发明人： Nikol De Forest ,  Werner Frei ,  Diego Rey ,  Shaunak Roy ,  Soni Shukla ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Victor H. Yee

IPC分类号： C12Q1/04 ,  C12Q1/68 ,  B01L3/00 ,  G01N21/64 ,  G01N21/76 ,  C12Q1/6897 ,  G01N21/03 ,  G01N21/77

摘要： An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

公开(公告)号：US09540675B2

公开(公告)日：2017-01-10

申请号：US14617631

申请日：2015-02-09

申请人： GeneWeave Biosciences, Inc.

发明人： Nikol De Forest ,  Werner Frei ,  Diego Rey ,  Shaunak Roy ,  Soni Shukla ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Victor H. Yee

IPC分类号： B01L3/00 ,  C12Q1/04 ,  C12Q1/68

CPC分类号： C12Q1/04 ,  B01L3/502 ,  B01L3/50825 ,  B01L3/523 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2300/0867 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0683 ,  C12Q1/6897 ,  G01N21/6486 ,  G01N21/76 ,  G01N21/77 ,  G01N2021/0325 ,  G01N2021/0328 ,  G01N2021/6443 ,  G01N2021/7786

摘要： An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

摘要翻译： 一种装置包括壳体和致动器。 限定可容纳试剂容器的试剂体积的外壳可以可拆卸地联接到反应室。 壳体包括定影与试剂体积流体连通的传送路径的穿刺器。 当壳体联接到反应室时，壳体的传送部分限定了传送路径和反应室之间的传送路径。 致动器具有设置在试剂体积内的柱塞部分。 可以操纵致动器的接合部分以使试剂体积内的柱塞部分移动以使试剂容器变形。 穿刺器可以刺穿试剂容器的易碎部分，以将试剂从试剂容器经由转移通路和/或输送通道输送到反应室中。