公开(公告)号：US20110059867A1

公开(公告)日：2011-03-10

申请号：US12714024

申请日：2010-02-26

申请人： Jinsang Kim ,  Jiseok Lee

发明人： Jinsang Kim ,  Jiseok Lee

IPC分类号： C40B40/06 ,  C07D405/00 ,  C07H21/00 ,  C07K2/00 ,  C07K16/00 ,  C12N9/00 ,  C07H3/00 ,  C40B40/04 ,  C40B40/10 ,  C40B40/12 ,  C07D251/30 ,  G01N30/96

CPC分类号： G01N33/5432 ,  C07B2200/11 ,  C07C233/20 ,  C07C233/49 ,  C07C233/55 ,  C07C251/48 ,  C07D251/30 ,  C07H21/00 ,  C40B40/04 ,  C40B80/00 ,  G01N33/52 ,  G01N33/542 ,  G01N33/582

摘要： A microarray includes a solid substrate having a surface, the surface having a plurality of binding spots and a plurality of reaction moieties bound to the binding spots. A reaction moiety includes a plurality of polyacetylene monomers, the polyacetylene monomers having a first coupling region and a second coupling region, the first coupling region having a first functional group operable to bind to the binding spot and the second coupling region comprising a second functional group operable to bind to an accessory molecule; and an accessory molecule having a binding region and an analyte reaction region, the analyte reaction region operable to selectively bind to the target analyte, and the binding region operable to bind to the second coupling region of the polyacetylene monomer. Upon binding a target analyte with the reaction moiety, a color change from the polyacetylene monomer occurs and the reaction moiety produces fluorescence.

摘要翻译： 微阵列包括具有表面的固体基质，该表面具有多个结合点和与结合点结合的多个反应部分。 反应部分包括多个聚乙炔单体，所述聚乙炔单体具有第一耦合区域和第二耦合区域，所述第一耦合区域具有可操作以结合至所述结合点的第一官能团，所述第二耦合区域包含第二官能团 可操作地结合辅助分子; 以及具有结合区域和分析物反应区域的辅助分子，所述分析物反应区域可操作以选择性地结合靶分析物，以及结合区域可操作以结合聚乙炔单体的第二耦合区域。 在将目标分析物与反应部分结合时，发生来自聚乙炔单体的颜色变化，并且反应部分产生荧光。

公开(公告)号：US20100291700A1

公开(公告)日：2010-11-18

申请号：US12799689

申请日：2010-04-28

申请人： Scot Weinberger ,  Stephen Dotson ,  Nathan Harris

发明人： Scot Weinberger ,  Stephen Dotson ,  Nathan Harris

IPC分类号： G01N21/00 ,  G01N30/96

CPC分类号： G01N33/54393 ,  G01N21/45 ,  G01N33/5432 ,  G01N33/54373 ,  G01N33/92

摘要： Devices and methods for detecting interaction between components associated with lipid membranes and analytes are described herein. In certain methods, a surface of a compartment of a device is coated with a material that attaches to lipid membrane from a sample. An analyte is introduced and interaction is detected, for example, by back scattering interferometry. In another method, a surface is passivated with a material that does not bind a lipid membrane. A sample is introduced that contains a membrane comprising a component for testing and an analyte. Interaction is detected. In a third method, a channel is filled with a first liquid that does not comprise a lipid membrane. A bolus of a second liquid that comprises a membrane comprising a component for testing and an analyte is introduced under laminar flow conditions so that a leading edge of the second liquid does not completely displace the first liquid in a sensing area that is interrogated by optical methods, for example, a laser. Interaction between the analyte and the component is detected.

摘要翻译： 本文描述了用于检测与脂质膜和分析物相关的组分之间相互作用的装置和方法。 在某些方法中，装置的隔室的表面涂覆有从样品附着到脂质膜的材料。 引入分析物并检测相互作用，例如通过反向散射干涉测量。 在另一种方法中，用不结合脂质膜的材料钝化表面。 引入了包含含有用于测试的组分和分析物的膜的样品。 检测到相互作用。 在第三种方法中，通道填充有不包含脂质膜的第一液体。 在层流条件下引入包括包含用于测试的组分的膜和分析物的第二液体的推注，使得第二液体的前缘不会在通过光学方法询问的感测区域中完全置换第一液体 ，例如，激光。 检测分析物与组分之间的相互作用。

公开(公告)号：US20100267166A1

公开(公告)日：2010-10-21

申请号：US12823961

申请日：2010-06-25

申请人： Albert Nazareth ,  Timothy Snowden ,  Yea-shun Cheng

发明人： Albert Nazareth ,  Timothy Snowden ,  Yea-shun Cheng

IPC分类号： G01N33/558 ,  G01N30/96

CPC分类号： G01N33/558 ,  G01N2333/59 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/805 ,  Y10S436/81

摘要： The invention comprises a device for detecting an analyte in a liquid sample deposited on a first portion of the device for transport to a second portion of the device that is in fluid contact with the first portion. In specific embodiments, the device comprises a labeled conjugate comprising a binding member reactive with a first epitope of the analyte and a label comprising a gold colloid, preferably having a mean particle size of 50 nm to 100 nm. In further embodiments, the device comprises a capture component comprising polymerized streptavidin. The diagnostic device is particularly useful in the preparation of pregnancy test kits.

公开(公告)号：US20100227323A1

公开(公告)日：2010-09-09

申请号：US12670893

申请日：2008-07-30

申请人： Antje Baeumner ,  Sam R. Nugen

发明人： Antje Baeumner ,  Sam R. Nugen

IPC分类号： C12Q1/68 ,  G01N30/96 ,  G01N33/543 ,  G01N33/53 ,  C12M1/34 ,  B05D3/14

CPC分类号： G01N27/44721 ,  G01N27/44747

摘要： The present invention relates to a device and methods for detecting or quantifying an analyte in a test sample. The device includes a substrate defining one or more microchannels and having a reaction region in a first portion of the one or more microchannels, wherein the reaction region contains a first binding element selected to bind with a first portion of the analyte. The device also includes a detection region in fluid communication with the reaction region. The detection region includes a second binding element selected to immobilize the analyte within the detection region. Methods of detecting or quantifying an analyte in a test sample using the device of the present invention are also disclosed. A method for coating a polymer with a gold layer is also disclosed.

摘要翻译： 本发明涉及用于检测或定量测试样品中的分析物的装置和方法。 该器件包括限定一个或多个微通道并且在一个或多个微通道的第一部分中具有反应区的衬底，其中反应区含有选择与被分析物的第一部分结合的第一结合元件。 该装置还包括与反应区域流体连通的检测区域。 检测区域包括选择用于将分析物固定在检测区域内的第二结合元件。 还公开了使用本发明的装置检测或定量测试样品中的分析物的方法。 还公开了用金层涂覆聚合物的方法。

公开(公告)号：US20100221815A1

公开(公告)日：2010-09-02

申请号：US12694678

申请日：2010-01-27

申请人： Nicholas Abbott ,  Christopher Murphy ,  Barbara Israel

发明人： Nicholas Abbott ,  Christopher Murphy ,  Barbara Israel

IPC分类号： C12M1/34 ,  G01N33/00 ,  G01N30/96

CPC分类号： G01N21/75 ,  B82Y30/00 ,  Y10T436/143333

摘要： The present invention relates to the field of molecular diagnostics, and in particular to diagnostics based on a liquid crystal assay format. In particular, the present invention provided improved substrates and methods of using liquid crystal assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal assay format.

摘要翻译： 本发明涉及分子诊断领域，特别涉及基于液晶测定形式的诊断。 特别地，本发明提供了改进的基板和使用液晶测定法来定量样品中分析物的量的方法。 本发明还提供了通过使用液晶测定形式来检测分析物与底物的非特异性结合的材料和方法。

公开(公告)号：US07763208B2

公开(公告)日：2010-07-27

申请号：US10978773

申请日：2004-11-01

申请人： John Carl Steichen ,  Harry Edwards Betsill

发明人： John Carl Steichen ,  Harry Edwards Betsill

IPC分类号： G01N30/96

CPC分类号： G01N33/0031

摘要： Disclosed herein is a device and method for converting the resistances of an array of metal oxide sensors into digital signals, wherein the sensors respond to the concentration of gases surrounding the sensor array. The device includes an electronic excitation device that provides an excitation voltage to the sensors; analog-to-digital signal conversion devices that convert the resistances of the sensors to digital signals; and means for calculating gas concentrations from the digital signals.

摘要翻译： 本文公开了一种用于将金属氧化物传感器阵列的电阻转换为数字信号的装置和方法，其中传感器响应于传感器阵列周围的气体浓度。 该装置包括向传感器提供激励电压的电子激励装置; 将传感器的电阻转换为数字信号的模拟 - 数字信号转换装置; 以及用于从数字信号计算气体浓度的装置。

公开(公告)号：US20100143196A1

公开(公告)日：2010-06-10

申请号：US12526936

申请日：2008-01-18

申请人： James Patrick Reed ,  Ray Stewart

发明人： James Patrick Reed ,  Ray Stewart

IPC分类号： G01N30/96 ,  B32B37/00

CPC分类号： G01N29/022 ,  G01N29/032 ,  G01N2291/0256 ,  G01N2291/0427 ,  Y10T156/10

摘要： Microcantilever sensors having protected microcantilever arms are described. The sensors are well suited for analyte detection and measuring other small movements. The protected arm reduces damage due to handling and assists in assembly of the microcantilever sensor with a transducer.

摘要翻译： 描述了具有受保护的微型悬臂的微悬臂传感器。 传感器非常适用于分析物检测和测量其他小的运动。 受保护的手臂可减少由于处理造成的损坏，并有助于使用传感器组装微型悬臂传感器。

公开(公告)号：US20100075859A1

公开(公告)日：2010-03-25

申请号：US12473789

申请日：2009-05-28

申请人： Caroline Garey ,  Jodie Hadley ,  Peter Swarbrick

发明人： Caroline Garey ,  Jodie Hadley ,  Peter Swarbrick

IPC分类号： C40B30/00 ,  G01N30/96 ,  G01N21/00 ,  G01N21/76 ,  C40B40/00

CPC分类号： G01N33/5432 ,  G01N33/54313 ,  G01N33/585 ,  G01N33/586

摘要： There is described a system for multiparameter analysis of analytes. The system comprises: 1) primary supports (1) with a largest dimension (3) of 500 μm or less suspended in use in a fluid solution; 2) each primary support (1) comprises an identification means (2) for identification thereof; 3) at least one primary analyte (12) is bound to each primary support (1); 4) a secondary analyte (12′) is suspended in use in the fluid solution; and 5) a measuring means (25) is arranged in communication with the fluid solution for monitoring interaction between the primary analyte (12) and secondary analyte (12′). The system is distinguished in that: 6) secondary supports (1′) with a largest dimension at the most the same size as the largest dimension (3) of the primary supports (1) are suspended in use in the fluid solution; 7) each secondary support (1′) comprises an identification means (2′) for identification thereof; 8) at least one secondary analyte (12′) is bound to each of the secondary supports (1′); and 9) the measuring means (25) is arranged to detect any post-reaction interaction between one or more primary analyte (12) and one or more secondary analyte (12′) by detecting the identification means (2, 2′) of the primary and secondary supports (1, 1′) attached thereto. There is also described a method of multiparameter analysis of analytes using the system.

摘要翻译： 描述了一种用于分析物多参数分析的系统。 该系统包括：1）在流体溶液中悬浮的最大尺寸（3）为500μm或更小的主要载体（1）; 2）每个主要支撑件（1）包括用于识别它的识别装置（2） 3）至少一个主要分析物（12）结合到每个主要载体（1）上; 4）第二分析物（12'）在使用中悬浮在流体溶液中; 和5）测量装置（25）被布置成与流体溶液连通，用于监测主要分析物（12）和第二分析物（12'）之间的相互作用。 该系统的特征在于：6）具有最大尺寸的与主要支撑件（1）的最大尺寸（3）最大尺寸的次级支撑件（1'）在使用中悬浮在流体溶液中; 7）每个辅助支撑件（1'）包括用于识别的识别装置（2'）; 8）至少一个次级分析物（12'）结合到每个辅助支撑件（1'）上; 和9），所述测量装置（25）被布置成通过检测所述第一分析物（12）的识别装置（2,2'）来检测一个或多个主要分析物（12）和一个或多个次要分析物（12'）之间的任何反应后相互作用 附加到其上的主和辅助支撑件（1,1'）。 还描述了使用该系统对分析物进行多参数分析的方法。

公开(公告)号：US20090317916A1

公开(公告)日：2009-12-24

申请号：US12213694

申请日：2008-06-23

申请人： Kenneth J. Ewing ,  Danielle N. Dickinson ,  Douglas B. Henderson ,  Johnny Ho

发明人： Kenneth J. Ewing ,  Danielle N. Dickinson ,  Douglas B. Henderson ,  Johnny Ho

IPC分类号： G01N30/96

CPC分类号： G01N1/405 ,  B01D53/0415 ,  B01D2253/102 ,  B01D2253/202 ,  B01D2253/204 ,  B01D2258/0225 ,  B01D2259/4583 ,  G01N1/2214 ,  G01N2001/022 ,  H01J49/0409

摘要： A chemical sample collection and detection system is disclosed. The chemical sample collection and detection system includes a sample collection device and a detection device. The sample collection device includes a housing having two opposite sides and at least one openings on each side to allow a fluid sample passing through the housing; and a sorbent material placed between the two opposite sides of the housing or a sorbent coated screen. The sorbent material adsorbs chemical vapors, and traps particles and aerosols in the fluid sample when the fluid sample passes the housing through the openings. The detection device includes an atmospheric pressure ionization source and an ion detector. The atmospheric pressure ionization source desorbs and ionizes the chemicals trapped/sorbed on the sorbent material and the ion detector analyzes the ions for the presence of the sorbed chemical.

摘要翻译： 公开了一种化学样品收集和检测系统。 化学样品收集和检测系统包括样品收集装置和检测装置。 样品收集装置包括具有两个相对侧的壳体和每侧上的至少一个开口，以允许流体样品​​通过壳体; 以及放置在壳体的两个相对侧或吸附剂涂覆的屏幕之间的吸附剂材料。 当流体样品通过开口通过壳体时，吸附剂材料吸附化学蒸气并捕获流体样品中的颗粒和气溶胶。 检测装置包括大气压电离源和离子检测器。 大气压离子源解吸并吸附在吸附剂材料上捕获/吸附的化学物质，离子检测器分析离子以吸附吸附剂的存在。

公开(公告)号：US20090305332A1

公开(公告)日：2009-12-10

申请号：US12478878

申请日：2009-06-05

申请人： Erich Haendler ,  Norbert Oranth

发明人： Erich Haendler ,  Norbert Oranth

IPC分类号： C12Q1/02 ,  G01N21/00 ,  C12M1/34 ,  G01N30/96

CPC分类号： G01N33/557 ,  G01N21/8483 ,  G01N33/558

摘要： A method for the determination of an analyte in a liquid sample, especially a body liquid sample, with the aid of an analytical apparatus. The apparatus receives a first measuring signal si (i=0, 1, . . . ) and a second measuring signal sj (j=1, 2, . . . ; j>i) for a test system with several successively arranged measuring areas through which a liquid sample with an analyte can flow after being applied on the test system are received by an evaluation apparatus comprised by the analytical apparatus, wherein the first measuring signal si indicates a part of the analyte bound in a first measuring area and the second measuring signal sj indicates a part of the analyte bound in a second measuring area that is arranged downstream the first measuring area on the test system after the application of the liquid sample on the test system, a concentration measure N correlating with the concentration of the analyte in the liquid sample is determined by the evaluation apparatus in accordance with the following linkage of measuring signals: N = ( s j ) i + 2 [ s i - ( ( s i ) j - i - 1 · s j ) 1 j - i ] · ( ( s i ) j - i - 1 · s j ) i j - 1 , and the concentration measurement is outputted by the evaluation apparatus after a selective further processing. Furthermore, the invention relates to an analytical apparatus for the determination of an analyte in a liquid sample as well as to a computer readable medium for performing function to determine an analyte in a liquid sample.

摘要翻译： 借助于分析装置测定液体样品，特别是体液样品中的分析物的方法。 该装置接收具有多个连续布置的测量区域的测试系统的第一测量信号si（i = 0,1等）和第二测量信号sj（j = 1,2，...，j> i） 通过分析装置包含的评估装置接收具有分析物的液体样品在被施加在测试系统上之后流动，其中第一测量信号si表示在第一测量区域中被结合的分析物的一部分，第二测量信号 测量信号sj表示在将液体样品施加到测试系统上之后，在测试系统上的第一测量区域下游布置的第二测量区域中结合的分析物的一部分，与分析物浓度相关的浓度测量N 在液体样品中，根据测量信号的以下连接，由评估装置确定：N =（sj）i + 2 [si - （（si）j - i - 1。sj）1 j - i]。 （（s i）j-i-1，s j）i j-1，并且在选择性进一步处理之后，由评估装置输出浓度测量值。 此外，本发明涉及一种用于确定液体样品中的分析物以及用于执行功能以确定液体样品中的分析物的计算机可读介质的分析装置。