公开(公告)号：US20230251234A1

公开(公告)日：2023-08-10

申请号：US18058698

申请日：2022-11-23

申请人： HITACHI HIGH-TECH SCIENCE CORPORATION

发明人： Masahito ITO ,  Norimasa MINAMOTO ,  Yusuke HOSEN ,  Masami SHIBUKAWA

IPC分类号： G01N30/96 ,  G01N30/54

CPC分类号： G01N30/96 ,  G01N30/54 ,  G01N2030/3076

摘要： Disclosed herein are an amino acid analysis method and a liquid chromatographic apparatus for improving separation performance of threonine, serine, glycine, and alanine. The method of analyzing amino acids using the liquid chromatographic apparatus equipped with a cation exchange column includes a process for distributing a sample containing threonine, serine, glycine, and alanine as the amino acids, together with an eluent, to the cation exchange column to separate threonine, serine, glycine, and alanine, wherein a column temperature when separating threonine and serine is higher than a column temperature when separating glycine and alanine.

公开(公告)号：US20230251231A1

公开(公告)日：2023-08-10

申请号：US18002533

申请日：2021-07-06

申请人： DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

发明人： Suya Liu ,  Takashi Baba

IPC分类号： G01N30/72 ,  G01N30/96 ,  G01N30/88 ,  G01N33/68

CPC分类号： G01N30/72 ,  G01N30/88 ,  G01N30/96 ,  G01N33/6848 ,  G01N2030/022

摘要： A separation time of an isomer of one or more isomers of a sialylated glycopeptide of a sample is calculated from a peak of a precursor XIC. Product ion intensities of the first group are summed at the separation time producing a first sum and product ion intensities of the second group are summed at the separation time producing a second sum using XICs of the first and second groups. A ratio of the first sum to the second sum is calculated. The ratio at the separation time is compared to predetermined ratio ranges that each corresponds to a combination of a selection from a set of the first linkage and the second linkage taken one or more times. One or more linkages of the sialic acid to the glycan of the isomer are identified from a combination found to match the ratio in the comparison.

公开(公告)号：US20230176019A1

公开(公告)日：2023-06-08

申请号：US17545249

申请日：2021-12-08

申请人： DIONEX CORPORATION

发明人： Yongjing Chen

IPC分类号： G01N30/96 ,  H01J49/04

CPC分类号： G01N30/96 ,  H01J49/0431 ,  G01N2030/965

摘要： The eluent used in IC separation contains non-volatile salt which is not compatible with electrospray ionization-mass spectrometry (ESI-MS). A suppressor is required to convert the non-volatile salt into water or the volatile acid form (i.e. acetic acid). When the suppressor fails, the non-volatile salts will enter the MS and cause extensive shutdown and maintenance of the mass spectrometer. The suppressor voltage derivative is used to evaluate the most common suppressor failure modes, including disruption of regenerant flow and excessive backpressure on the suppressor due to clogging in the downstream, and to trigger the eluent pump to stop the eluent flow or to trigger the auxiliary valve to switch the flow to the mass spectrometer from eluent to water.

公开(公告)号：US11598014B2

公开(公告)日：2023-03-07

申请号：US17061786

申请日：2020-10-02

申请人： DIONEX CORPORATION

发明人： Kannan Srinivasan

IPC分类号： C25B15/08 ,  C25B9/65 ,  C25B9/05 ,  C25B1/04 ,  C25B1/16 ,  C25B1/22 ,  C25B11/04 ,  C25B15/02 ,  G01N30/96 ,  G01N30/02 ,  G01N30/34

摘要： An ion source and ion recycle module includes an electrolyte reservoir, an eluent recovery chamber, and an ion exchange connector. The electrolyte reservoir includes a chamber containing an aqueous electrolyte solution including an electrolyte having a chamber inlet and a chamber outlet, and a first electrode. The chamber inlet is fluidically connected to a source chamber of an electrolytic eluent generator and configured to receive depleted electrolyte solution from the source chamber of the electrolytic eluent generator. The chamber outlet is fluidically connected to the source chamber of the electrolytic eluent generator and configured to provide recycled electrolyte solution to the electrolytic eluent generator source chamber. The eluent recovery chamber including a second electrode and configured to receive an eluent solution including eluent counter ions from the eluent generator; and the ion exchange connector including an ion exchange membrane stack.

公开(公告)号：US11564976B2

公开(公告)日：2023-01-31

申请号：US17136334

申请日：2020-12-29

申请人： CSL Behring Lengnau AG

发明人： Stefan Schulte ,  Hans-Wilhelm Beltz ,  Sabine Pestel ,  Thomas Weimer ,  Matthias Pelzing

IPC分类号： A61K38/00 ,  A61K38/37 ,  C07K14/755 ,  A61P7/04 ,  A61K38/17 ,  C07K14/47 ,  G01N30/96

摘要： The present invention provides modified von Willebrand Factor molecules, methods for their preparation and uses thereof. The invention further provides pharmaceutical compositions for treating coagulation disorders.

公开(公告)号：US20220236153A1

公开(公告)日：2022-07-28

申请号：US17577262

申请日：2022-01-17

申请人： DIONEX CORPORATION

发明人： Kannan SRINIVASAN

IPC分类号： G01N1/40 ,  G01N30/08 ,  G01N30/96 ,  G01N30/38 ,  G01N30/40 ,  B01D15/36

摘要： Systems and methods for concentrating an analyte preparatory to analysis thereof include processing the effluent of an analyte concentrator to produce an eluent for eluting an analyte retained in the same or separate concentrator, and systems implementing the same. The analyte concentrator system connects the effluent outlet of an analyte concentrator column to an eluent generation module such that the substantially analyte-free effluent discharged from the analyte concentrator column passes fluidly into the eluent generation module. Eluent generated from the substantially analyte-free effluent in the eluent generation module is likewise substantially free of the analyte. The systems and methods can minimize and/or (substantially) eliminate background signal during analysis of the concentrated analyte.

公开(公告)号：US20220146476A1

公开(公告)日：2022-05-12

申请号：US17441665

申请日：2019-03-27

申请人： SHIMADZU CORPORATION

发明人： Masanori FUJIWARA ,  Katsumasa SAKAMOTO

IPC分类号： G01N30/96 ,  G01N30/02

摘要： First and second electrode liquid seal members are arranged between a first electrode and a second electrode. First and second ion exchange membranes are arranged between a first electrode liquid seal member and a second electrode liquid seal member. An eluent seal member is arranged between a first ion exchange membrane and a second ion exchange membrane. Ion exchange is performed between an eluent that passes through an eluent flow path of the eluent seal member from a separation column and an electrode liquid that passes through each of electrode liquid flow paths of the first and second electrode liquid seal members. In a first surface of the eluent seal member that comes into contact with the first ion exchange membrane, a first projection that surrounds the entire circumference of the eluent flow path to extend along the edge of the eluent flow path and projects toward the first ion exchange membrane is formed.

公开(公告)号：US11312741B2

公开(公告)日：2022-04-26

申请号：US16094508

申请日：2017-04-19

申请人： GLYCOM A/S

发明人： Pierre Chassagne ,  Nikolay Khanzhin ,  Martin Matwiejuk ,  Markus Jondelius Hederos ,  Niels Banke

IPC分类号： C07H13/04 ,  B01D61/02 ,  B01D61/14 ,  B01D61/58 ,  C07H1/06 ,  C12P19/28 ,  B01D15/36 ,  B01J41/07 ,  B01D61/16 ,  B01J39/05 ,  B01D15/18 ,  B01J20/20 ,  G01N30/96 ,  C12P19/26 ,  G01N30/02

摘要： The invention relates to a method for obtaining an N-acetylglucosamine containing neutral oligosaccharide from a fermentation broth, wherein said oligosaccharide is produced by culturing a genetically modified microorganism capable of producing said oligosaccharide from an internalized carbohydrate precursor, comprising the steps of: i) ultrafiltration (UF), preferably to separate biomass from the broth, ii) nanofiltration (NF), preferably to concentrate said oligosaccharide in the broth and/or reduce an inorganic salt content of the broth, and iii) treating the broth with an ion exchange resin, preferably to remove charged materials, and/or subjecting the broth to chromatography, preferably to remove hydrophobic impurities.

公开(公告)号：US11287403B2

公开(公告)日：2022-03-29

申请号：US15377833

申请日：2016-12-13

申请人： Board of Regents, The University of Texas System

发明人： Purnendu K. Dasgupta ,  Charles Phillip Shelor ,  Hongzhu Liao

IPC分类号： G01N30/96 ,  G01N27/447 ,  B01D15/24 ,  B01D15/36

摘要： A permeative amine/acid introduction device (PAID) is placed after a conventional KOH eluent suppressed conductometric anion chromatography (SCAC) system. The PAID converts the suppressed eluites from the acid form to the corresponding salt. For example, when the analytes are acids, they are converted to the corresponding ammonium salt (NR2H+HX→NR2H2++X−) and allows very weak acids HX (pKa≥7.0) that cannot normally be detected by SCAC to be measured by a second conductivity detector following the PAID. Permeative reagent introduction is dilutionless, can be operated without pumps and provides good mixing with low band dispersion (as small as 30 μL). An exemplary amine is diethylamine (DEA), which was chosen as the amine source due to its low pKb value (pKb 3.0), high vapor pressure, and low toxicity and low odor.

公开(公告)号：US11249060B2

公开(公告)日：2022-02-15

申请号：US16605704

申请日：2018-07-16

申请人： LG Chem, Ltd.

发明人： Sangwoo Kim ,  Hyun Kyung Yoon ,  Sumin Na ,  Jieun Kim ,  Hyun Woo Nho

IPC分类号： G01N31/12 ,  G01N30/06 ,  G01N30/14 ,  G01N1/22 ,  G01N30/88 ,  G01N30/96

摘要： The present invention relates to a method for quantitatively analyzing Cl, remaining after synthesis, in zinc ferrite synthesized using chloride precursors such as zinc chloride and iron chloride, and provides a method capable of using, in a quantitative analysis method of Cl remaining after synthesis of an inorganic material, AQF-IC, which has been used only in the quantitative analysis of an organic sample since gaseous Cl, discharged after burning zinc ferrite in an automatic quick furnace (AQF) by using an Sn capsule and tungsten oxide (WO3), is analyzed through ion chromatography (IC).