公开(公告)号：US20170321220A1

公开(公告)日：2017-11-09

申请号：US15587098

申请日：2017-05-04

Applicant: INDIANA UNIVERSITY RESEARCH AND TECHNOLOGY CORPORATION

Inventor： Trevor DOUGLAS ,  Benjamin Harold SCHWARZ ,  Ranjit KOLIYATT ,  Masaki UCHIDA ,  John Alexander AVERA

IPC: C12N15/62 ,  C12N15/63 ,  C07K14/435 ,  C12N15/10 ,  C12Q1/68 ,  C12N15/00 ,  C07K19/00

CPC classification number: C12N15/62 ,  C07K14/005 ,  C07K14/435 ,  C07K14/70578 ,  C07K19/00 ,  C07K2319/00 ,  C07K2319/21 ,  C12N15/00 ,  C12N15/1062 ,  C12N15/1075 ,  C12N15/63 ,  C12N2795/10222 ,  C12N2795/10223 ,  C12Q1/68

Abstract: The invention provides a fusion protein comprising an antigen binding domain linked to a bacteriophage decoration (Dec) protein along with a polynucleotide comprising the nucleic acid sequence of the fusion protein and a vector comprising the polynucleotide. Additionally, the invention provides a composition comprising the fusion protein and a virus-like particle (VLP), and a method of treating a disease in a mammal comprising administering a therapeutically effective amount of the composition to the mammal. The invention also provides a method of vaccinating against a disease comprising administering a composition comprising the fusion protein and a VLP encapsulating a protein.

公开(公告)号：US09803193B2

公开(公告)日：2017-10-31

申请号：US14796480

申请日：2015-07-10

Applicant: X-Body, Inc.

Inventor： Richard W. Wagner ,  Alexander Litovchick ,  Yan Chen

IPC: C12N15/10 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  C07K19/00

CPC classification number: C12N15/1086 ,  C07K19/00 ,  C07K2317/10 ,  C07K2317/40 ,  C07K2317/56 ,  C12N15/1062 ,  C12N15/1075 ,  G01N33/5308 ,  G01N33/54366 ,  G01N33/6845

Abstract: The invention provides methods and compositions useful for identifying polypeptides with desired characteristics in vitro.

公开(公告)号：US09753040B2

公开(公告)日：2017-09-05

申请号：US13990654

申请日：2011-11-30

Applicant: Yasuhiro Fujino ,  Risako Fujita ,  Kouichi Wada ,  Kotomi Oda ,  Takuya Ueda ,  Yoshihiro Shimizu ,  Takashi Kanamori

Inventor： Yasuhiro Fujino ,  Risako Fujita ,  Kouichi Wada ,  Kotomi Oda ,  Takuya Ueda ,  Yoshihiro Shimizu ,  Takashi Kanamori

IPC: G01N33/68 ,  C12N15/10 ,  C07K16/46

CPC classification number: G01N33/6854 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/55 ,  C12N15/1041 ,  C12N15/1062 ,  C12N15/1075 ,  G01N33/6857

Abstract: The polynucleotide construct of (1) or (2) below is used to perform ribosome display, CIS display and/or mRNA display in order to screen a Fab against an antigen of interest: (1) a polynucleotide construct which monocistronically comprises a ribosome-binding sequence, Fab first chain-coding sequence, linker peptide-coding sequence, Fab second chain-coding sequence and scaffold-coding sequence in this order, and further comprises at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself; and (2) a polynucleotide construct which comprises a Fab first chain-expressing cistron and a Fab second chain-expressing cistron each containing a ribosome-binding sequence, a Fab first chain-coding sequence or Fab second chain-coding sequence, and a scaffold-coding sequence in this order, the first Fab-expressing cistron further comprising at its 3′-end a ribosome stall sequence, said Fab second chain-expressing cistron further comprising at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself.

公开(公告)号：US20170081658A1

公开(公告)日：2017-03-23

申请号：US15367255

申请日：2016-12-02

Applicant: John CHAPUT ,  Andrew LARSEN ,  Annabelle GILLIG

Inventor： John CHAPUT ,  Andrew LARSEN ,  Annabelle GILLIG

IPC: C12N15/10 ,  C12N15/67

CPC classification number: C12N15/1055 ,  C12N15/1062 ,  C12N15/67

Abstract: The present invention provides methods for rapidly screening and measuring the ligand binding affinity of in vitro selected peptides to the cognate and off-target proteins. This general strategy is amenable to high throughput analysis because the peptides are synthesized by cell-free translation, as opposed to solid-phase synthesis required by traditional assays, and affinities can be readily measured in standard formats.

公开(公告)号：US20170037109A1

公开(公告)日：2017-02-09

申请号：US15237484

申请日：2016-08-15

Applicant: BRISTOL-MYERS SQUIBB COMPANY

Inventor： Jonathan DAVIS ,  Dasa Lipovsek ,  Ray Camphausen

IPC: C07K14/78 ,  G01N33/68

CPC classification number: C07K14/78 ,  A61K38/00 ,  C07K14/47 ,  C07K16/241 ,  C07K16/244 ,  C07K16/28 ,  C07K16/2833 ,  C07K16/2857 ,  C07K2317/92 ,  C07K2318/20 ,  C12N15/1062 ,  C40B40/10 ,  G01N33/68 ,  G01N33/6887

Abstract: Fibronectin type III (10Fn3) binding domains having novel designs that are associated with reduced immunogenicity are provided. The application describes alternative 10Fn3 binding domains in which certain immunogenic regions are not modified when producing a binder in order to maintain recognition as a self antigen by the host organism. The application also describes 10Fn3 binding domains in which HLA anchor regions have been destroyed thereby reducing the immunogenic contribution of the adjoining region. Also provided are 10Fn3 domains having novel combinations of modified regions that can bind to a desired target with high affinity.

Abstract translation: 提供了具有与降低的免疫原性相关的新颖设计的纤连蛋白III型（10Fn3）结合结构域。 本申请描述了替代的10Fn3结合结构域，其中当产生粘合剂时，某些免疫原性区域不被修饰，以便由宿主生物保持识别为自身抗原。 该应用还描述了其中HLA锚定区已经被破坏的10Fn3结合结构域，从而降低相邻区域的免疫原性贡献。 还提供了10Fn3结构域，其具有可以高亲和力结合所需靶的修饰区域的新颖组合。

公开(公告)号：US09518152B2

公开(公告)日：2016-12-13

申请号：US14008982

申请日：2012-04-02

Applicant: Takamitsu Yano

Inventor： Takamitsu Yano

IPC: C07D403/14 ,  C08G73/06 ,  A61K31/785 ,  A61K31/787 ,  C12N15/10 ,  A61K47/48

CPC classification number: A61K31/4178 ,  A61K31/785 ,  A61K31/787 ,  A61K47/595 ,  C07D403/14 ,  C08G73/0611 ,  C08G73/0616 ,  C12N15/1062 ,  C12Q2600/178

Abstract: The object of the present invention is to provide a fundamental therapy to mitochondrial genetic diseases caused by mutation of the mitochondrial (mt)DNA, and a pharmaceutical composition used for the same. The object can be solved by a polyamide compound binding to a target double-stranded mtDNA comprising A/T pair consisting of first A of the following sense-stranded DNA and the corresponding T, A/T pair consisting of 8th A of the following sense-stranded DNA and the corresponding T, G/C pair consisting of 9th G of the following sense-stranded DNA and the corresponding C, G/C pair consisting of 14th G of the following sense-stranded DNA and the corresponding C, T/A pair consisting of 15th T of the following sense-stranded DNA and the corresponding A, or the like, in the double-stranded DNA consisting of the sense-stranded DNA having base sequence of 5′-ATGGCAGAGCCCGGTAATCGCATAA-3′ (SEQ ID NO: 1) and the antisense-stranded DNA having base sequence of 5′-TTATGCGATTACCGGGCTCTGCCAT-3′ (SEQ ID NO: 2).

Abstract translation: 本发明的目的是提供对由线粒体（mt）DNA的突变引起的线粒体遗传病的基础疗法和用于其的药物组合物。 该目的可以通过结合到包含由以下正义链DNA的第一个A组成的A / T对的对象双链mtDNA的聚酰胺化合物和由以下意义的第8位组成的对应的T，A / T对 并且相应的T，G / C对由以下正义链DNA的第9G组成的相应的C，G / C对由以下正义链DNA的第14位和对应的C，T / 由具有5'-ATGGCAGAGCCCGGTAATCGCATAA-3'（SEQ ID NO：3）的碱基序列的有义链DNA组成的双链DNA中的由第15位以下的正义链和相应的A等构成的对 ：1）和具有5'-TTATGCGATTACCGGGCTCTGCCAT-3'（SEQ ID NO：2）的碱基序列的反义链DNA。

公开(公告)号：US09416170B2

公开(公告)日：2016-08-16

申请号：US13757664

申请日：2013-02-01

Applicant: BRISTOL-MYERS SQUIBB COMPANY

Inventor： Jonathan Davis ,  Dasa Lipovsek ,  Ray Camphausen

IPC: C40B40/10 ,  C07K14/78 ,  C07K14/47 ,  G01N33/68 ,  C12N15/10 ,  C07K16/24 ,  C07K16/28

CPC classification number: C07K14/78 ,  A61K38/00 ,  C07K14/47 ,  C07K16/241 ,  C07K16/244 ,  C07K16/28 ,  C07K16/2833 ,  C07K16/2857 ,  C07K2317/92 ,  C07K2318/20 ,  C12N15/1062 ,  C40B40/10 ,  G01N33/68 ,  G01N33/6887

Abstract: Fibronectin type III (10Fn3) binding domains having novel designs that are associated with reduced immunogenicity are provided. The application describes alternative 10Fn3 binding domains in which certain immunogenic regions are not modified when producing a binder in order to maintain recognition as a self antigen by the host organism. The application also describes 10Fn3 binding domains in which HLA anchor regions have been destroyed thereby reducing the immunogenic contribution of the adjoining region. Also provided are 10Fn3 domains having novel combinations of modified regions that can bind to a desired target with high affinity.

公开(公告)号：US09410148B2

公开(公告)日：2016-08-09

申请号：US13821940

申请日：2011-09-08

Applicant: Hiroaki Suga ,  Yusuke Yamagishi

Inventor： Hiroaki Suga ,  Yusuke Yamagishi

IPC: C12N15/10 ,  C40B40/10 ,  C40B30/04 ,  C40B50/06 ,  C07K2/00 ,  C12P21/02 ,  C40B20/04 ,  C12N15/67

CPC classification number: C12N15/1062 ,  C07K2/00 ,  C12N15/67 ,  C12P21/02 ,  C40B20/04 ,  C40B30/04 ,  C40B40/10 ,  C40B50/06 ,  G01N2500/00 ,  G01N2500/20

Abstract: A method for screening a non-standard peptide compound in the peptide library that binds to the target substance, comprising the steps: (i) preparing a non-standard peptide library wherein a special (non-standard) amino acid is randomly incorporated into the peptide sequence by a cell-free (in vitro) translation system comprising a tRNA acylated by a special (non-standard) amino acid; (ii) bringing the obtained peptide library in contact with a target substance; and (iii) selecting a non-standard peptide that binds to the target substance as an active peptide.

Abstract translation: 一种用于筛选结合靶物质的肽文库中的非标准肽化合物的方法，包括以下步骤：（i）制备非标准肽文库，其中将特殊（非标准）氨基酸随机并入到 通过包含通过特殊（非标准）氨基酸酰化的tRNA的无细胞（体外）翻译系统的肽序列; （ii）使获得的肽文库与目标物质接触; 和（iii）选择与靶物质结合的非标准肽作为活性肽。

公开(公告)号：US20160097050A1

公开(公告)日：2016-04-07

申请号：US14890864

申请日：2014-05-15

Applicant: THE BOARD OF TRUSTEES OF THE LELAND

Inventor： William J. Greenleaf ,  Curtis J. Layton

IPC: C12N15/10

CPC classification number: C12N15/1041 ,  C12N15/1058 ,  C12N15/1062 ,  C12N15/1075 ,  C40B30/04 ,  C40B50/06 ,  C40B50/08

Abstract: A novel method for displaying proteins and peptides is disclosed in which individual proteins or peptides remain associated with the DNA encoding them. Proteins or peptides can be generated by in vitro translation of DNA templates, either free in solution or arrayed on a solid support, such that the proteins or peptides remain immobilized on their DNA templates. In particular, high throughput sequencing can be combined with high throughput functional characterization of encoded proteins and peptides, wherein the identity of each protein or peptide is determined by DNA sequencing, and functional studies are carried out directly on each protein or peptide while immobilized on the DNA template encoding it. The methods of the invention should find numerous applications, for example, in high throughput genetic or pharmacological screening, epitope mapping, and protein engineering and directed evolution.

Abstract translation: 公开了一种显示蛋白质和肽的新方法，其中单独的蛋白质或肽与编码它们的DNA保持相关联。 蛋白质或肽可以通过体外翻译DNA模板产生，DNA模板在溶液中游离或排列在固体支持物上，使得蛋白质或肽保持固定在DNA模板上。 特别地，高通量测序可以与编码的蛋白质和肽的高通量功能表征组合，其中通过DNA测序确定每个蛋白质或肽的同一性，并且功能研究直接在每种蛋白质或肽上进行，同时固定在 DNA模板编码。 本发明的方法应当发现许多应用，例如在高通量遗传或药理学筛选，表位作图，蛋白质工程和定向进化中。

公开(公告)号：US20160076022A1

公开(公告)日：2016-03-17

申请号：US14849992

申请日：2015-09-10

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Takanori Ichiki ,  Shingo Ueno ,  Manish Biyani ,  Ryo Kobayashi ,  Hirofumi Shiono

IPC: C12N15/10

CPC classification number: C12N15/1062 ,  C12N15/1093 ,  C12Q1/6853 ,  C12Q2521/107 ,  C12Q2565/537

Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.

Abstract translation: 本发明涉及用于产生mRNA复合物的核酸连接体和由mRNA编码的蛋白质或肽，所述连接体包含：5'末端的间隔部分; 可与mRNA序列的至少一部分杂交的多核苷酸部分; 以及臂部，其具有在3'末端的蛋白质或肽的连接部分，其中间隔部分，多核苷酸部分和臂部分形成单链，并且其中多核苷酸部分含有光反应性 基础衍生物。