公开(公告)号：US09767343B1

公开(公告)日：2017-09-19

申请号：US15221285

申请日：2016-07-27

Applicant: Medica Corporation

Inventor： Ronald Jones ,  Adrian Gropper ,  Robert Hagopian ,  Charles Rogers ,  Thomas Vitella ,  Tyler Cote ,  Donald Barry ,  Dirk Osterloh ,  Chen Yi

IPC: G01N33/49 ,  G01N33/80 ,  G01N15/10 ,  G01N1/10 ,  G01N1/30 ,  G01N21/05 ,  G06K9/00 ,  G01N33/487 ,  G01N33/50 ,  G01N15/00

CPC classification number: G06K9/00134 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0627 ,  B01L2400/0633 ,  B01L2400/0644 ,  G01N1/10 ,  G01N1/30 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1484 ,  G01N21/05 ,  G01N33/487 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/5094 ,  G01N33/80 ,  G01N2015/0065 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2035/00148 ,  G06K9/00147 ,  Y10T436/101666

Abstract: This disclosure describes single-use test cartridges, cell analyzer apparatus, and methods for automatically performing microscopic cell analysis tasks, such as counting blood cells in biological samples. A small unmeasured quantity of a biological sample such as whole blood is placed in the disposable test cartridge which is then inserted into the cell analyzer. The analyzer isolates a precise volume of the biological sample, mixes it with self-contained reagents and transfers the entire volume to an imaging chamber. The geometry of the imaging chamber is chosen to maintain the uniformity of the mixture, and to prevent cells from crowding or clumping, when it is transferred into the imaging chamber. Images of essentially all of the cellular components within the imaging chamber are analyzed to obtain counts per unit volume. The devices, apparatus and methods described may be used to analyze a small quantity of whole blood to obtain counts per unit volume of red blood cells, white blood cells, including sub-groups of white cells, platelets and measurements related to these bodies.

公开(公告)号：US20170254739A1

公开(公告)日：2017-09-07

申请号：US15510104

申请日：2015-09-09

Applicant: Universiteit Leiden ,  Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.

Inventor： Sanli Ebrahimi Pour Faez ,  Vahid Sandoghdar

IPC: G01N15/14 ,  G01N21/47 ,  G01N21/53 ,  G01N21/64

CPC classification number: G01N15/1436 ,  B01L3/502761 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/474 ,  G01N21/53 ,  G01N21/6458 ,  G01N2015/0038 ,  G01N2021/4742 ,  G01N2201/08

Abstract: There is provided a particle detection apparatus (30) comprising: a channel (32) including an inlet and at least one channel wall, the inlet permitting light to be introduced into the channel (32), the or each channel wall being arranged to define a channel path through which light may propagate; a light source (34) configured to introduce light into the channel (32) via the inlet, the channel (32) being shaped to guide the light to propagate along the channel path for illuminating a particle or a plurality of particles located in the channel path; and a monitoring device (36) configured to detect scattered light that is created by the illumination of the or each particle by the guided light and that leaves the channel (32) by passing through the or each channel wall.

公开(公告)号：US20170239658A1

公开(公告)日：2017-08-24

申请号：US15519515

申请日：2015-10-16

Applicant: Ezra S. ABRAMS ,  Christian T. BOLES ,  Yu CHEN ,  James STURM ,  Robert AUSTIN ,  SAGE SCIENCE, INC. ,  TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Ezra S. ABRAMS ,  T. Christian BOLES ,  Yu CHEN ,  James STURM ,  Robert AUSTIN

IPC: B01L3/00 ,  G01N27/447 ,  G01N15/14 ,  C12Q1/68

CPC classification number: B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2400/0421 ,  B01L2400/0487 ,  C12Q1/6806 ,  G01N15/1484 ,  G01N27/44791 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2015/1497 ,  C12Q2523/308 ,  C12Q2565/629

Abstract: In some embodiments, a method for manipulating DNA molecules for use in a microfluidic device is provided, where the method may comprise providing a solution of a plurality of DNA molecules having a first radius of gyration under under a zero flow velocity, and maintaining the DNA molecules in a spherical shape under a flow velocity.

公开(公告)号：US09702808B2

公开(公告)日：2017-07-11

申请号：US15377869

申请日：2016-12-13

Applicant: Namocell Inc.

Inventor： Junyu Lin

IPC: G01N15/00 ,  G01N15/14 ,  B01L3/00 ,  G01N15/10

CPC classification number: G01N15/1484 ,  B01L3/0241 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  G01N15/1459 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149

Abstract: Methods and apparatuses for sorting and isolating individual microparticles (e.g., cells) by bulk sorting groups of microparticles into groups containing one or more microparticle having a desirable characteristic (e.g., a label such as a florescent label, shape, size, etc.), and then sorting the individual microparticles with this desired characteristic individual particles.

公开(公告)号：US09689863B2

公开(公告)日：2017-06-27

申请号：US13789799

申请日：2013-03-08

Applicant: Chang He Bio-Medical Science (Yangzhou) Co., Ltd

Inventor： Chris C Yu ,  Xuedong Du ,  He Yu

IPC: B01L3/00 ,  G01N33/50 ,  G01N15/14 ,  G01N15/02 ,  G01N15/10 ,  G01N35/00

CPC classification number: G01N33/5091 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2400/0487 ,  G01N15/1056 ,  G01N15/1484 ,  G01N2015/0288 ,  G01N2015/1006 ,  G01N2035/00158 ,  Y10T436/143333

Abstract: Among others, the present invention provides micro-devices for detecting or treating a disease, each comprising a first sorting unit capable of detecting a property of the biological subject at the microscopic level and sorting the biological subject by the detected property; a first detection unit capable of detecting the same or different property of the sorted biological subject at the microscopic level; and a first layer of material having an exterior surface and an interior surface, wherein the interior surface defines a first channel in which the biological subject flows from the first sorting unit to the first detection unit.

公开(公告)号：US09683984B2

公开(公告)日：2017-06-20

申请号：US14330062

申请日：2014-07-14

Applicant: Avishay Bransky ,  Max Herzberg

Inventor： Avishay Bransky ,  Max Herzberg

IPC: C12M1/34 ,  G01N33/49 ,  G01N15/14 ,  B01L3/00 ,  B01F13/00 ,  F21V8/00

CPC classification number: G01N33/4915 ,  B01F13/0064 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2300/0867 ,  G01N15/1404 ,  G01N15/147 ,  G01N15/1475 ,  G01N15/1484 ,  G01N2015/1497 ,  G02B6/0021 ,  G02B6/0038

Abstract: An apparatus arranged for examining particles, comprising: a cartridge having at least one microchannel, a viscoelastic fluid flowing in the microchannel, the fluid comprising a suspension of particles, thereby effecting alignment of the particles in at least one-dimensional array parallel to the fluid flow, and an optical magnifying means generating an image of the particles in the microchannel.

公开(公告)号：US20170167966A1

公开(公告)日：2017-06-15

申请号：US15387034

申请日：2016-12-21

Applicant: PREMIUM GENETICS (UK) LTD.

Inventor： David Appleyard ,  Jeff Betthauser ,  Marjorie Faust ,  John Larsen ,  Guocheng Shao ,  Zheng Xia ,  Yu Zhou

IPC: G01N15/14 ,  G01N21/01 ,  G01N33/483 ,  G01N1/44

CPC classification number: G01N15/1484 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0627 ,  C12N13/00 ,  G01N1/44 ,  G01N15/1404 ,  G01N21/01 ,  G01N21/6486 ,  G01N33/4833 ,  G01N2015/1006 ,  G01N2015/1409 ,  G01N2015/1413

Abstract: An apparatus and method of identifying objects includes: a microfluidic chip in which are disposed a plurality of channels, the micro-fluidic chip including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, the sheath fluids which orient the objects in the main fluid channel in a predetermined direction while still maintaining laminar flow in the main fluid channel; an interrogation apparatus which detects and interrogates the oriented objects in the main fluid channel; and a focused energy apparatus which performs an action on the objects.

公开(公告)号：US20170122861A1

公开(公告)日：2017-05-04

申请号：US15318542

申请日：2014-07-18

Applicant: NAMOCELL INC.

Inventor： Junyu LIN

IPC: G01N15/14 ,  B01L3/00

CPC classification number: G01N15/1484 ,  B01L3/0241 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2300/0816 ,  G01N15/1459 ,  G01N15/147 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149

Abstract: Apparatus and methods for sorting and dispensing microparticles using a flow switch mechanism wherein changing flow rate into the flow switch changes flow path. The present invention is well-suited for precisely sorting microparticles, such as cells, for applications such as cell line development, monoclonal antibody selection and single cell research, etc.

公开(公告)号：US09638622B2

公开(公告)日：2017-05-02

申请号：US14652433

申请日：2013-10-15

Applicant: SONY CORPORATION

Inventor： Katsutoshi Tahara

IPC: G01N15/14 ,  G01N21/49 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/49 ,  G01N2015/0053 ,  G01N2015/1006

Abstract: A particle detection apparatus and a particle detection method capable of accurately detecting scattered light are provided. A scattered light detection unit of the particle detection apparatus having at least a light illumination unit for illuminating a particle with light and the scattered light detection unit for detecting scattered light emitted from the particle illuminated with light is provided with a first detection portion for detecting scattered light, a second detection portion for detecting illumination light onto a particle, and a signal processing unit for removing a noise component from a signal detected in the first detection portion based on a signal detected in the second detection portion.

公开(公告)号：US09618442B2

公开(公告)日：2017-04-11

申请号：US14739812

申请日：2015-06-15

Applicant: CYTONOME/ST, LLC

Inventor： Johnathan Charles Sharpe ,  Emanuel Tito Mendes Machado ,  Blair D. Morad ,  Rudolf Hulspas ,  Donald Francis Perrault, Jr.

IPC: G01N21/00 ,  G01N15/14 ,  G01N15/10 ,  G01N21/53 ,  G01N21/64

CPC classification number: G01N15/1484 ,  G01N15/1012 ,  G01N15/1056 ,  G01N15/1429 ,  G01N15/1459 ,  G01N21/53 ,  G01N21/64 ,  G01N2015/1018 ,  G01N2015/1075 ,  G01N2015/1402 ,  G01N2015/149

Abstract: A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.