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    Rapid whole genome amplification
    41.
    发明授权
    Rapid whole genome amplification 有权
    快速全基因组扩增

    公开(公告)号：US09428801B2

    公开(公告)日：2016-08-30

    申请号：US13339633

    申请日：2011-12-29

    申请人： Mark Eshoo ,  Stanley Motley ,  John Picuri

    发明人： Mark Eshoo ,  Stanley Motley ,  John Picuri

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/686 ,  C12P19/34 ,  C12Q1/6846 ,  C12Q2521/525 ,  C12Q2525/179 ,  C12Q2527/125

    摘要： The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.

    摘要翻译： 本发明提供用于快速扩增允许使用少量起始核酸的靶核酸（例如，使用全基因组扩增）的组合物和方法。 在某些实施方案中，所述方法使用包含以下的组合物：phi29聚合酶，ex-Klenow聚合酶和/或Klenow聚合酶，dNTP，引物和缓冲剂。 在一些实施方案中，以在30分钟内至少产生1000倍扩增的速率扩增靶核酸。

    Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby
    46.
    发明授权
    Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby 有权
    定义数据库的二级结构和用于确定未知生物标签的身份和地理起源的方法

    公开(公告)号：US08921047B2

    公开(公告)日：2014-12-30

    申请号：US13663176

    申请日：2012-10-29

    申请人： Ibis Biosciences, Inc.

    发明人： David J. Ecker ,  Richard H. Griffey ,  Rangarajan Sampath ,  Steven A. Hofstadler ,  John McNeil ,  Stanley T. Crooke

    IPC分类号： C12Q1/68 ,  G01N30/72 ,  G06F19/18 ,  G06F19/10 ,  G06F19/00

    CPC分类号： G06F19/28 ,  G06F19/00 ,  G06F19/10 ,  G06F19/16 ,  G06F19/18 ,  G16H50/80 ,  Y02A90/24 ,  Y02A90/26

    摘要： The present invention relates generally to the field of investigational bioinformatics and more particularly to secondary structure defining databases. The present invention further relates to methods for interrogating a database as a source of molecular masses of known bioagents for comparing against the molecular mass of an unknown or selected bioagent to determine either the identity of the selected bioagent, and/or to determine the origin of the selected bioagent. The identification of the bioagent is important for determining a proper course of treatment and/or irradication of the bioagent in such cases as biological warfare. Furthermore, the determination of the geographic origin of a selected bioagent will facilitate the identification of potential criminal identity.

    摘要翻译： 本发明一般涉及研究生物信息学领域，更具体地涉及定义数据库的二级结构。 本发明还涉及询问数据库作为已知生物标签的分子量来源的方法，用于与未知或选择的生物标签的分子量进行比较以确定所选择的生物标签的身份，和/或确定所选生物标记的来源 所选的生物标签。 生物制剂的鉴定对于在诸如生物战争的情况下确定生物制剂的适当治疗过程和/或辐照是重要的。 此外，确定所选择的生物制剂的地理起源将有助于确定潜在的犯罪认同。

    NUCLEIC ACID SEQUENCING BY ELECTROCHEMICAL DETECTION
    48.
    发明申请
    NUCLEIC ACID SEQUENCING BY ELECTROCHEMICAL DETECTION 有权
    通过电化学检测进行核酸序列测序

    公开(公告)号：US20140238859A1

    公开(公告)日：2014-08-28

    申请号：US14237328

    申请日：2012-08-03

    申请人： Jeffrey Huff ,  Graham Davis ,  Mark Hayden ,  David J. Ecker ,  Dan Wang ,  Gordon Bruce Collier

    发明人： Jeffrey Huff ,  Graham Davis ,  Mark Hayden ,  David J. Ecker ,  Dan Wang ,  Gordon Bruce Collier

    IPC分类号： C12Q1/68 ,  G01N27/447

    CPC分类号： C12Q1/6869 ,  G01N27/3275 ,  G01N27/333 ,  G01N27/447 ,  G01N27/44756 ,  Y02A50/59 ,  Y02A50/60 ,  C12Q2565/607 ,  C12Q2563/113 ,  C12Q2563/116

    摘要： Provided herein is technology relating to sequencing nucleic acids and particularly, but not exclusively, to devices, methods, and systems for sequencing-by-synthesis using changes in pH to monitor base addition. In some embodiments the electrochemical hydrogen ion sensor is a microfabricated mixed metal oxide electrode and in some embodiments the electrochemical hydrogen ion sensor is a membrane electrode. Moreover, in some embodiments the device further comprises a reference electrode. Performing the sequencing reaction involves moving solutions and other fluids (e.g., samples, nucleotide solutions, wash solutions) into and out of the reaction vessel. Thus, in some embodiments, the device further comprises a tube or other transport mechanism or pathway attached to the reaction vessel.

    摘要翻译： 本文提供了与测序核酸有关的技术，特别但并非排他地涉及使用pH变化监测碱添加的用于按合成顺序进行测序的装置，方法和系统。 在一些实施方案中，电化学氢离子传感器是微制造的混合金属氧化物电极，并且在一些实施方案中，电化学氢离子传感器是膜电极。 此外，在一些实施例中，该装置还包括参考电极。 执行测序反应包括将溶液和其它流体（例如，样品，核苷酸溶液，洗涤溶液）移入和移出反应容器。 因此，在一些实施例中，该装置还包括连接到反应容器的管或其它输送机构或通路。

    SAMPLE PROCESSING UNITS, SYSTEMS, AND RELATED METHODS
    49.
    发明申请
    SAMPLE PROCESSING UNITS, SYSTEMS, AND RELATED METHODS 审中-公开
    样品加工单元，系统和相关方法

    公开(公告)号：US20140224042A1

    公开(公告)日：2014-08-14

    申请号：US14102200

    申请日：2013-12-10

    申请人： IBIS BIOSCIENCES, INC.

    发明人： Steven A. Hofstadler ,  Jared J. Drader ,  Jose R. Gutierrez ,  Paul J. Gleason ,  Rex O. Bare ,  Robert D. Miller ,  Jeffrey C. Smith

    IPC分类号： G01N1/10

    CPC分类号： G01N1/10 ,  G01N1/02 ,  G01N35/0098 ,  G01N35/025 ,  G01N2035/00524

    摘要： Sample processing units useful for mixing and purifying materials, such as fluidic materials are provided. A sample processing unit typically includes a container configured to contain a sample comprising magnetically responsive particles, and one or more magnets that are in substantially fixed positions relative to the container. A sample processing unit also generally includes a conveyance mechanism configured to convey the container to and from a position that is within magnetic communication with the magnet, e.g., such that magnetically responsive particles with captured analytes can be retained within the container when other materials are added to and/or removed from the container. Further, a sample processing unit also typically includes a rotational mechanism that is configured to rotate the container, e.g., to effect mixing of sample materials disposed within the container. Related carrier mechanisms, sample processing stations, systems, and methods are also provided.

    摘要翻译： 提供了用于混合和净化材料（如流体材料）的样品处理单元。 样品处理单元通常包括被配置为容纳包含磁响应颗粒的样品和相对于容器处于基本固定位置的一个或多个磁体的容器。 样品处理单元还通常包括输送机构，其构造成将容器输送到与磁体磁连通的位置，例如使得当添加其它材料时，具有捕获的分析物的磁响应颗粒可以保留在容器内 到和/或从容器中取出。 此外，样品处理单元还通常包括旋转机构，其被配置为旋转容器，例如以实现设置在容器内的样品材料的混合。 还提供了相关的载体机构，样品处理站，系统和方法。