公开(公告)号：US20230384302A1

公开(公告)日：2023-11-30

申请号：US18139242

申请日：2023-04-25

申请人： Inguran, LLC

发明人： Kristie Krug

IPC分类号： G01N33/543 ,  B03C1/015 ,  B03C1/02 ,  B03C1/023 ,  B03C1/01 ,  B03C1/28 ,  C12N13/00 ,  G01N33/569

CPC分类号： G01N33/5434 ,  B03C1/015 ,  B03C1/02 ,  G01N33/54346 ,  G01N33/54353 ,  B03C1/023 ,  B03C1/01 ,  B03C1/288 ,  G01N33/54333 ,  C12N13/00 ,  G01N33/56966 ,  B03C2201/18 ,  B03C2201/26

摘要： A method for magnetic cellular manipulation may include contacting a composition with a biological sample to form a mixture. The composition may include a plurality of particles. Each particle in the plurality of particles may include a magnetic substrate. The magnetic substrate may be characterized by a magnetic susceptibility greater than zero. The composition may also include a chargeable silicon-containing compound. The chargeable silicon-containing compound may coat at least a portion of the magnetic substrate. The biological sample may include cells and/or cellular structures. The method may also include applying a magnetic field to the mixture to manipulate the composition.

公开(公告)号：US11821897B2

公开(公告)日：2023-11-21

申请号：US16448571

申请日：2019-06-21

申请人： Hoffmann-La Roche Inc.

发明人： Regula Buser ,  Sandra Grau-Richards ,  Virginie Steinhart

IPC分类号： G01N33/542 ,  C12N15/85 ,  G01N21/64 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58 ,  G01N33/53 ,  G01N33/536 ,  G01N33/533

CPC分类号： G01N33/542 ,  C12N15/85 ,  G01N21/6408 ,  G01N21/6428 ,  G01N33/53 ,  G01N33/533 ,  G01N33/536 ,  G01N33/54306 ,  G01N33/56966 ,  G01N33/56972 ,  G01N33/586 ,  G01N2021/6439

摘要： The present invention relates to a new cell based assay for determining antibody or ligand binding and function using lipid-like compounds capable of spontaneous integration into cell membranes.

公开(公告)号：US20230313134A1

公开(公告)日：2023-10-05

申请号：US18182829

申请日：2023-03-13

申请人： THE UNIVERSITY OF SHEFFIELD

发明人： Carlos Marcelo Nicolas Rivolta ,  Sarah Louise Boddy

IPC分类号： C12N5/0797 ,  C07K16/28 ,  C07K16/30 ,  C07K16/18 ,  G01N33/569 ,  G01N33/48 ,  G01N33/53

CPC分类号： C12N5/0623 ,  C07K16/2896 ,  C07K16/3084 ,  C07K16/18 ,  G01N33/56966 ,  G01N33/48 ,  G01N33/53 ,  C07K16/28 ,  G01N2333/70596 ,  G01N2333/7452 ,  G01N2333/71 ,  G01N2333/916 ,  G01N2333/705

摘要： The present invention relates generally to the identification and isolation of human otic progenitor cells. More specifically, the present invention relates to a method of using cell markers to identify and isolate human otic progenitor cells from a mixed population of cells, methods of enrichment and production of human otic progenitor cells, and associated kits for use in identification and/or isolation of human otic progenitor cells, wherein the cell markers are selected from SSEA1 (CD15), disialoganglioside GD3, TRA-2-49 (liver/bone/kidney alkaline phosphatase), SSEA4, ganglioside GD2 and CD141.

公开(公告)号：US11774444B2

公开(公告)日：2023-10-03

申请号：US16349235

申请日：2017-11-10

申请人： Beth Israel Deaconess Medical Center, Inc.

发明人： Ionita C. Ghiran ,  Nathan Shapiro ,  Anish V. Sharda ,  Edward J. Felton

IPC分类号： G01N33/543 ,  B03C1/01 ,  B03C1/033 ,  B03C1/30 ,  B03C1/32 ,  G01N9/36 ,  G01N33/50 ,  G01N35/00 ,  G01N33/569 ,  G01N33/537

CPC分类号： G01N33/54326 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/30 ,  B03C1/32 ,  G01N9/36 ,  G01N33/5005 ,  G01N33/5044 ,  G01N33/537 ,  G01N33/56966 ,  G01N33/56972 ,  G01N35/0098 ,  B03C2201/26

摘要： Methods and apparatus for detecting cell-bound and soluble antigens in a biological sample are described. The method comprises forming complexes of at least one antibody-coated bead and at least one antigen in a solution, placing the solution in a magnetic field such that the formed complexes levitate in the solution at a particular height, and determining at least one characteristic of the antigen in the complexes based, at least in part, on an image of the complexes showing the magnetic levitation height.

公开(公告)号：US11732018B2

公开(公告)日：2023-08-22

申请号：US16937619

申请日：2020-07-24

申请人： International Institute Of Cancer Immunology, Inc.

发明人： Haruo Sugiyama

IPC分类号： A61K38/00 ,  C07K14/47 ,  G01N33/574 ,  A61K39/00 ,  C07K16/18 ,  C07K16/30 ,  C12N5/078 ,  G01N33/569

CPC分类号： C07K14/4748 ,  A61K39/001153 ,  C07K14/4702 ,  C07K16/18 ,  C07K16/30 ,  C12N5/0634 ,  G01N33/56966 ,  G01N33/57438 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/55566

摘要： The present invention relates to a WT1 peptide which has an amino acid sequence consisting of contiguous amino acids derived from a WT1 protein and induces WT1-specific helper T cells by binding to an MHC class II molecule, a pharmaceutical composition comprising them and the like.

公开(公告)号：US11692174B2

公开(公告)日：2023-07-04

申请号：US16484167

申请日：2018-02-08

申请人： Sapphiros AI Bio LLC

发明人： Robin Y. Smith ,  Marcie A. Glicksman

IPC分类号： G01N33/48 ,  C12N5/074 ,  G16B45/00 ,  G16B35/10 ,  G01N33/569

CPC分类号： C12N5/0696 ,  G01N33/56966 ,  G16B35/10 ,  G16B45/00 ,  C12N2501/999 ,  C12N2510/00 ,  C12N2510/02

摘要： Described herein are various systems, methods, and apparatus for systematic creation of isolated homogeneous colonies of cells from vector-based lineages. The vector-based lineages may originate from multiple types of viral vector families (e.g., Paramyx-oviridae, Retroviridae, Parvoviridae) or non-natural engineered vectors or a plurality of vector combinations, for example. In certain embodiments, the isolated homogeneous colonies of cells are vector-free sub-colonies; in other embodiments, the isolated homogeneous colonies of cells are homogeneous vector sub-colonies. In other embodiments, vector mixed sub-colonies are created. The disclosed systems, methods, and apparatus are useful for inducible pluripotent stem cell (iPSC) production and work by selectively binding to one or more corresponding protein markers expressed on the surface of a cell that indicate that cellular reprogramming has occurred. Software is used to automate the purification and isolation of the iPSCs produced.

公开(公告)号：US20230184776A1

公开(公告)日：2023-06-15

申请号：US18063678

申请日：2022-12-08

申请人： Duke University ,  Menarini Silicon Biosystems S.p.A.

发明人： Galla Chandra Rao ,  Mark C. Connelly ,  Mariano A. Garcia-Blanco ,  Andrew J. Armstrong ,  Rhonda L. Bitting

IPC分类号： G01N33/574 ,  G01N33/543 ,  G01N33/569

CPC分类号： G01N33/57484 ,  G01N33/54326 ,  G01N33/56966 ,  G01N33/57415 ,  G01N33/57434 ,  G01N33/57492 ,  G01N2333/70589 ,  G01N2333/47 ,  G01N2333/4703 ,  G01N2333/4742 ,  G01N2333/705 ,  G01N2333/70503 ,  G01N2333/70596

摘要： Provided are methods for detecting or isolating circulating tumor cells (CTCs) in a subject. The methods may include detecting the expression of at least one epithelial mesenchymal transition (EMT) biomarker. Further provided are kits for detecting or isolating CTCs. The kits may include antibodies to at least one EMT biomarker. Further provided are methods of predicting the responsiveness of a subject to a cancer drug, methods of targeting delivery of a cancer drug in a subject, methods of providing a cancer prognosis to a subject, and methods for following the progress of cancer in a subject.

公开(公告)号：US20230160889A1

公开(公告)日：2023-05-25

申请号：US16975009

申请日：2019-03-18

申请人： SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION

发明人： Sung Hoon KWON ,  Dae Won LEE ,  Yong Ju LEE ,  Ok Ju KIM ,  Choong Won LEE

IPC分类号： G01N33/569 ,  G01N15/14 ,  G01N33/543

CPC分类号： G01N33/56966 ,  G01N15/1463 ,  G01N15/1434 ,  G01N33/54313 ,  G01N33/54386 ,  G01N2015/1006

摘要： The present invention relates to a method and system for screening and separating biological specimens. The screening and separating method and system provide reliable results based on information related to the types, shapes, and positions of labeled products provided by an agent for accurate screening. In addition, the information related to the types, shapes, and positions can be combined to determine objective indices for the state of a target specimen, enabling rapid selection of the target specimen. The method and system for screening and separating biological specimens according to the present invention use an agent optimized to accurately and effectively create information on the selection of a target specimen and process images based on information on images stored in a server, enabling sensitive and highly reproducible screening evaluation.

公开(公告)号：US20230143550A1

公开(公告)日：2023-05-11

申请号：US17689869

申请日：2022-03-08

申请人： JANSSEN BIOTECH, INC.

发明人： Rebecca HAWKINS ,  Steven JACOBS ,  Manuel SEPULVEDA

IPC分类号： C07K14/78 ,  G01N33/569 ,  G01N33/68 ,  G01N33/566 ,  A61K51/08 ,  G01N33/53 ,  A61K49/00 ,  G01N33/574

CPC分类号： C07K14/78 ,  G01N33/56966 ,  G01N33/6887 ,  G01N33/566 ,  A61K51/088 ,  G01N33/53 ,  A61K49/0002 ,  G01N33/68 ,  G01N33/569 ,  G01N33/57492 ,  G01N33/574 ,  G01N2333/70517 ,  G01N2333/78

摘要： Fibronectin type III domains (FN3) that specifically bind to CD8A, related polynucleotides capable of encoding CD8A-specific FN3 domains, cells expressing the FN3 domains, as well as associated vectors, and detectably labeled FN3 domains are useful in therapeutic and diagnostic applications.

公开(公告)号：US20190247030A1

公开(公告)日：2019-08-15

申请号：US15894949

申请日：2018-02-13

申请人： TrophoDiagnostics, LLC

发明人： Zitao Liu ,  Lian Liu

IPC分类号： A61B10/02 ,  G01N33/569 ,  C07K16/28 ,  G01N1/40 ,  G01N1/34 ,  C12Q1/6883 ,  A61B5/00

CPC分类号： A61B10/0291 ,  A61B5/4362 ,  C07K16/2833 ,  C12Q1/6883 ,  C12Q2600/156 ,  G01N1/34 ,  G01N1/405 ,  G01N33/56966

摘要： Improved systems and methods for collecting, isolating and analyzing trophoblasts from the endocervical canal for prenatal genetic testing are disclosed. Particularly, an endocervical sample collection device is disclosed, where that collection device is enhanced with surface nanostructures/microstructures and functionalized with trophoblast specific antibodies to allow the collection device to preferentially adhere to and pick up fetal trophoblasts as opposed to maternal cells and/or non-cellular materials such as endocervical mucus. In addition, a non-caustic mucolytic preparation for treating endocervical sample to remove mucus while keeping the trophoblast cells intact and viable for downstream culturing and processing is disclosed.