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    Cellular diagnostic arrays, methods of using and processing for producing same
    11.
    发明申请
    Cellular diagnostic arrays, methods of using and processing for producing same 有权
    蜂窝诊断阵列，使用和处理方法来生产它

    公开(公告)号：US20020142285A1

    公开(公告)日：2002-10-03

    申请号：US09965644

    申请日：2001-09-27

    Inventor： Mark W. Bitensky ,  Tatsuro Yoshida ,  Michael G. Frank ,  Christopher V. Gabel ,  Jure Derganc

    IPC: C12Q001/00 ,  C12M001/34

    CPC classification number: C12M41/46 ,  B01J2219/00596 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/0074 ,  C12M23/16 ,  G01N15/1463 ,  G01N33/54366 ,  G01N2015/0076 ,  G01N2015/1486 ,  G01N2015/1495 ,  G01N2015/1497

    Abstract: A cellular diagnostic array has been developed that provides accurate and reproducible measurements of the physical parameters of individual cells en masse, including cell membrane surface area, cell volume, and excess membrane. Three differing patterns are employed. In the first design, a gradient array having rectangular shaped channels is used to capture cells and readily show the ratio of volume to surface area for the captured cell. The second silicone rubber array provided by the present invention employs an array of wedge shaped pipettes, each of which captures an individual cell. The position of the cell within a wedge is enough to rapidly determine the cell's actual surface area and volume. A third type of cellular diagnostic array according to the present invention employs an array of channels designed to deform a cell to study how well the cell responds to repeated deformations.

    Abstract translation: 已经开发了一种细胞诊断阵列，其提供了对各个细胞的物理参数的精确和可再现的测量，包括细胞膜表面积，细胞体积和过量的膜。 采用三种不同的模式。 在第一种设计中，使用具有矩形通道的梯度阵列来捕获细胞并且容易地显示捕获的细胞的体积与表面积的比率。 由本发明提供的第二硅橡胶阵列采用楔形移液管阵列，其中每一个捕获单个细胞。 电池在楔形件内的位置足以快速确定电池的实际表面积和体积。 根据本发明的第三类型的细胞诊断阵列使用设计成使细胞变形以研究细胞对重复变形的响应程度的通道阵列。

    METHOD AND DEVICE FOR HIGH THROUGHPUT CELL DEPORMABILITY MEASUREMENTS
    16.
    发明申请
    METHOD AND DEVICE FOR HIGH THROUGHPUT CELL DEPORMABILITY MEASUREMENTS 审中-公开
    用于高通量细胞可分量测量的方法和装置

    公开(公告)号：US20160231224A1

    公开(公告)日：2016-08-11

    申请号：US14552256

    申请日：2014-11-24

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T.K. Tse

    IPC: G01N15/14 ,  G01N33/487 ,  B01L3/00

    CPC classification number: G01N15/1463 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502776 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0864 ,  C12Q1/02 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/487 ,  G01N33/574 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/149 ,  G01N2015/1495 ,  G01N2015/1497 ,  G01N2800/7028 ,  G06F19/10

    Abstract: A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.

    Abstract translation: 公开了一种能够以高产量自动测量细胞机械参数的系统。 微流体装置使用相交流来产生细胞经受受控拉伸的拉伸流动区域。 在进入延伸流动区域之前，细胞被聚焦成流线。 在拉伸区域中，每个单元的变形用成像装置测量。 自动图像分析从图像中提取一系列独立的生物力学参数。 这些可能包括细胞大小，变形能力和圆形度。 然后可以以各种方式使用所获得的单细胞数据。 可以为用户开发和显示可变形性和圆形度的散射密度图。 诸如可变形性和圆形度的机械参数可以被门控或阈值化以识别感兴趣的某些细胞或感兴趣的亚群。 类似地，使用该设备获得的机械数据可以用作单元签名。

    Micro-optical cavity with fluidic transport chip for bioparticle analysis
    18.
    发明授权
    Micro-optical cavity with fluidic transport chip for bioparticle analysis 有权
    具有流体输送芯片的微光腔用于生物粒子分析

    公开(公告)号：US09063117B2

    公开(公告)日：2015-06-23

    申请号：US13337051

    申请日：2011-12-24

    Applicant: Paul L. Gourley

    Inventor： Paul L. Gourley

    IPC: G01N33/48 ,  G01N33/483 ,  G01N15/14

    CPC classification number: G01N33/4833 ,  G01N15/1404 ,  G01N15/1436 ,  G01N15/1484 ,  G01N21/47 ,  G01N21/6428 ,  G01N21/7746 ,  G01N33/574 ,  G01N2015/1006 ,  G01N2015/1409 ,  G01N2015/1493 ,  G01N2015/1495

    Abstract: This invention provides new methods and apparatus for rapidly analyzing single bioparticles to assess their material condition and health status. The methods are enabled by a resonant cavity to measure optical properties related to the bioparticle size and refractive index. Refractive index measurements are useful for determining material properties and biomolecular composition of the bioparticle. These properties and composition are dependent on the health state of the bioparticle. Thus, measured optical properties can be used to differentiate normal (healthy) and abnormal (diseased) states of bioparticles derived from cells or tissues. The methods are illustrated with data obtained from a resonator with a gain medium. The invention also provides new methods for multiple measurements in a single device, analyzing and manipulating bioparticles that are much smaller than the wavelength of light, and provides a microfluidic transport chip to enable rapid single bioparticle analysis of large populations of bioparticles.

    Abstract translation: 本发明提供用于快速分析单个生物颗粒以评估其物质状况和健康状况的新方法和装置。 该方法通过谐振腔来实现，以测量与生物颗粒尺寸和折射率相关的光学性质。 折射率测量可用于确定生物颗粒的材料性质和生物分子组成。 这些性质和组成取决于生物颗粒的健康状态。 因此，可以使用测量的光学性质来区分来自细胞或组织的生物颗粒的正常（健康）和异常（患病）状态。 用具有增益介质的谐振器获得的数据说明了这些方法。 本发明还提供了在单个装置中进行多次测量的新方法，分析和操作比光的波长小得多的生物颗粒，并且提供了一种微流控输送芯片，以实现大量生物颗粒的快速单一生物颗粒分析。

    BLOOD FLUIDITY MEASUREMENT APPARATUS AND BLOOD FLUIDITY MEASUREMENT METHOD
    19.
    发明申请
    BLOOD FLUIDITY MEASUREMENT APPARATUS AND BLOOD FLUIDITY MEASUREMENT METHOD 审中-公开
    血液流体测量装置和血液流量测量方法

    公开(公告)号：US20150098626A1

    公开(公告)日：2015-04-09

    申请号：US14568972

    申请日：2014-12-12

    Applicant: Konica Minolta, Inc.

    Inventor： Shuji ICHITANI ,  Osamu TOYAMA

    IPC: G01N11/04 ,  G01N33/49 ,  G06T7/20 ,  G01F3/36

    CPC classification number: G01N11/04 ,  G01F3/36 ,  G01N15/1463 ,  G01N33/49 ,  G01N2015/1075 ,  G01N2015/1495 ,  G06T7/215 ,  G06T2207/30104

    Abstract: Deformability of blood cells is quantified in a short time. A blood fluidity measurement apparatus is provided with a TV camera which photographs a stream of blood in either two areas of the internal area, entrance area, and exit area of a gate, an image processing part which calculates the velocity of the blood cells contained in the blood from the image taken by the TV camera, and a deformability calculation means which calculates the deformability of the blood cells a blood fluidity from the velocity.

    Abstract translation: 在短时间内量化血细胞的变形性。 血液流动性测量装置设置有电视摄像机，其对门的内部区域，入口区域和出口区域的两个区域中的血液流进行拍摄，图像处理部分计算包含在血液中的血细胞的速度 来自电视摄像机拍摄的图像的血液，以及根据该速度计算血液细胞变形性的变形能力计算装置。

    SYSTEM AND METHOD FOR DEFORMING AND ANALYZING PARTICLES
    20.
    发明申请
    SYSTEM AND METHOD FOR DEFORMING AND ANALYZING PARTICLES 有权
    用于变形和分析颗粒的系统和方法

    公开(公告)号：US20140315287A1

    公开(公告)日：2014-10-23

    申请号：US14058028

    申请日：2013-10-18

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T.K. Tse ,  Aram Chung

    IPC: G01N15/14

    CPC classification number: G01N15/1404 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/147 ,  G01N15/1484 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/50 ,  G01N33/5091 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/1495 ,  G01N2021/6439 ,  G06K9/4604 ,  G06T7/0004 ,  G06T7/0016

    Abstract: A system for deforming and analyzing a plurality of particles carried in a sample volume includes a substrate defining an inlet, configured to receive the sample volume, and an outlet; and a fluidic pathway fluidly coupled to the inlet and the outlet. The fluidic pathway includes a delivery region configured to receive the plurality of particles from the inlet and focus the plurality of particles from a random distribution to a focused state, a deformation region defining an intersection located downstream of the delivery region and coupled to the outlet, and wherein the deformation region is configured to receive the plurality of particles from the delivery region and to transmit each particle in the plurality of particles into the intersection from a single direction, a first branch fluidly coupled to the deformation region and configured to transmit a first flow into the intersection, and a second branch fluidly coupled to the deformation region and configured to transmit a second flow, substantially opposing the first flow, into the intersection, wherein the first flow and the second flow are configured to induce extension of one or more particles in the plurality of particles.

    Abstract translation: 用于变形和分析载体在样品体积中的多个颗粒的系统包括限定入口的衬底，其构造成接收样品体积和出口; 以及流体连接到入口和出口的流体通路。 流体通道包括配置成从入口接收多个颗粒并将多个颗粒从随机分布聚焦到聚焦状态的输送区域，限定位于输送区域下游并且耦合到出口的交叉的变形区域， 并且其中所述变形区域被配置为从所述递送区域接收所述多个颗粒并且将多个颗粒中的每个颗粒从单个方向传播到所述交叉点，所述第一分支流体耦合到所述变形区域并且被配置为传输第一 流入所述十字路口的第二分支和流体耦合到所述变形区域并被配置为将基本上与所述第一流相对的第二流传送到所述交叉点的第二分支，其中所述第一流和所述第二流被配置为诱导一个或多个 多个颗粒中的颗粒。

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