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公开(公告)号：US20180056293A1
公开(公告)日：2018-03-01
申请号：US15791068
申请日：2017-10-23
Applicant: President and Fellows of Harvard College
Inventor： David A. Weitz , Adam R. Abate
CPC classification number: B01L3/502761 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/502784 , B01L2200/0636 , B01L2200/0652 , B01L2300/0681 , Y10T137/0318 , Y10T137/8593
Abstract: The present invention is generally related to systems and methods for producing droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, at least one droplet is used to create a plurality of droplets, using techniques such as flow-focusing techniques. In one set of embodiments, a plurality of droplets, containing varying species, can be divided to form a collection of droplets containing the various species therein. A collection of droplets, according to certain embodiments, may contain various subpopulations of droplets that all contain the same species therein. Such a collection of droplets may be used as a library in some cases, or may be used for other purposes.
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公开(公告)号：US09897532B2
公开(公告)日：2018-02-20
申请号：US14552256
申请日：2014-11-24
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Daniel R. Gossett , Henry T. K. Tse
IPC: G01N15/14 , G06F19/10 , G01N33/574 , C12Q1/02 , B01L3/00 , G01N33/487 , G01N15/00 , G01N15/10
CPC classification number: G01N15/1463 , B01L3/502715 , B01L3/50273 , B01L3/502776 , B01L2200/0605 , B01L2200/0647 , B01L2200/0652 , B01L2300/0654 , B01L2300/0864 , C12Q1/02 , G01N15/1404 , G01N15/1459 , G01N15/1484 , G01N33/487 , G01N33/574 , G01N2015/0065 , G01N2015/1006 , G01N2015/1415 , G01N2015/149 , G01N2015/1495 , G01N2015/1497 , G01N2800/7028 , G06F19/10
Abstract: A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.
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公开(公告)号：US20180045708A1
公开(公告)日：2018-02-15
申请号：US15790981
申请日：2017-10-23
Applicant: ANGLE North America, Inc.
Inventor： Georgi HVICHIA
CPC classification number: B01L3/502753 , B01L2200/0652 , B01L2200/12 , B01L2300/0816 , B01L2300/0864 , C12N5/0694 , C12Q1/24 , G01N15/0272 , G01N33/491 , G01N33/574 , G01N2015/0288
Abstract: The disclosure relates to an apparatus for segregating particles on the basis of their ability to flow through a stepped passageway. At least some of the particles are unable to pass through a narrower passageway bounded by a segregating step, resulting in segregation of the particles. The breadth of the leading edge of at least one step of the apparatus is significantly greater than the overall width of the passageway in which the step occurs, permitting high and rapid sample throughput. The apparatus and methods described herein can be used to segregate particles of a wide variety of types. By way of example, they can be used to segregate circulating tumor cells from a human blood sample.
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94.发明申请公开(公告)号：US20180045638A1
公开(公告)日：2018-02-15
申请号：US15724561
申请日：2017-10-04
Applicant: SONY CORPORATION
Inventor： FUMITAKA OTSUKA , HIROTO KASAI , TAKASHI MIYATA , TAKAYUKI KATO , KOUHEI HATAMOTO
CPC classification number: G01N15/1404 , B01L3/0268 , B01L3/502715 , B01L3/502776 , B01L2200/0652 , B01L2300/0645 , B01L2300/0816 , B01L2300/0864 , B01L2400/0415 , B01L2400/0439 , B01L2400/049 , B07C5/3425 , G01N15/1459 , G01N2015/0019 , G01N2015/1006 , G01N2015/1406 , G01N2015/149
Abstract: The present disclosure provides a particle sorting apparatus, a particle sorting method, and a non-transitory computer-readable storage medium storing program that enable sorting object particles to be sorted with high precision, even when the sorting object particles are large. In the particle sorting apparatus, a charging unit that applies charges to at least a part of liquid droplets ejected from an orifice to generate a fluid stream and a charging control unit that adjusts a charge application end time in the charging unit according to sizes of particles included in the liquid droplets are provided.
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95.发明申请公开(公告)号：US20180036732A1
公开(公告)日：2018-02-08
申请号：US15785191
申请日：2017-10-16
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Daniel R. Gossett , Henry T.K. Tse
CPC classification number: B01L3/502776 , B01D12/00 , B01L3/502746 , B01L3/502761 , B01L2200/0652 , B01L2300/0816 , B01L2300/0864 , B01L2400/0487 , B01L2400/084 , G01N2015/149
Abstract: A method of exchanging fluids with suspended particles includes providing a microfluidic device with a first inlet channel operatively coupled to a source of particles and a second inlet channel operatively coupled to an exchange fluid. A transfer channel is connected at a proximal end to the first inlet channel and the second inlet channel. First and second outlet channels are connected to a distal end of the transfer channel. The source of particles is flowed at a first flow rate into the first inlet channel while the exchange fluid is flowed at a second flow rate into the second inlet channel wherein the ratio of the second flow rate to the first flow rate is at least 1.5. Particles are collected in one of the first and second outlet channels while fluid substantially free of particles is collected in the other of the first and second outlet channels.
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Patent Agency Ranking
公开(公告)号：US09878327B2
公开(公告)日：2018-01-30
申请号：US15062413
申请日：2016-03-07
Applicant: The General Hospital Corporation
Inventor： Kyle C. Smith , Ramin Haghgooie , Thomas Alan Barber , Ismail Emre Ozkumur , Ravi Kapur , Mehmet Toner
IPC: G01N15/06 , G01N33/00 , G01N33/48 , B01L3/00 , G01N1/40 , G01N33/543 , B03C1/28 , G01N33/487 , B03C1/033
CPC classification number: B01L3/502761 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/06 , B01L2300/12 , B01L2400/043 , B03C1/0332 , B03C1/288 , B03C2201/18 , B03C2201/26 , G01N1/4077 , G01N33/48735 , G01N33/54333
Abstract: This disclosure describes microfluidic devices that include one or more magnets, each magnet being operable to emit a magnetic field; and a magnetizable layer adjacent to the one or more magnets, in which the magnetizable layer is configured to induce a gradient in the magnetic field of at least one of the magnets. For example, the gradient can be at least 103 T/m at a position that is at least 20 μm away from a surface of the magnetizable layer. The magnetizable layer includes a first high magnetic permeability material and a low magnetic permeability material arranged adjacent to the high magnetic permeability material. The devices also include a microfluidic channel arranged on a surface of the magnetizable layer, wherein a central longitudinal axis of the microfluidic channel is arranged at an angle to or laterally offset from an interface between the high magnetic permeability material and the low magnetic permeability material.
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公开(公告)号：US20180021774A1
公开(公告)日：2018-01-25
申请号：US15722796
申请日：2017-10-02
Applicant: International Business Machines Corporation
Inventor： Joshua T. Smith , Cornelia K. Tsang , Chao Wang , Benjamin H. Wunsch
CPC classification number: B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L2200/0652 , B01L2200/0689 , B01L2200/12 , B01L2300/0887 , B01L2300/0896 , B01L2300/12 , B01L2400/086 , B81B7/0061 , B81B2201/058 , B81B2203/0338 , B81C1/00309 , B81C2203/0118 , G01N15/0255 , G01N15/10 , G01N2015/0288 , G01N2015/1081
Abstract: Techniques for use of wafer bonding techniques for sealing of microfluidic chips are provided. In one aspect, a wafer bonding sealing method includes the steps of: forming a first oxide layer coating surfaces of a first wafer, the first wafer having at least one fluidic chip; forming a second oxide layer on a second wafer; and bonding the first wafer to the second wafer via an oxide-to-oxide bond between the first oxide layer and the second oxide layer to form a bonded wafer pair, wherein the second oxide layer seals the at least one fluidic chip on the first wafer. The second wafer can be at least partially removed after performing the bonding, and fluidic ports may be formed in the second oxide layer. A fluidic chip device is also provided.
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公开(公告)号：US09861983B2
公开(公告)日：2018-01-09
申请号：US15434223
申请日：2017-02-16
Applicant: ANGLE North America, Inc.
Inventor： Georgi Hvichia
CPC classification number: B01L3/502753 , B01L2200/0652 , B01L2200/12 , B01L2300/0816 , B01L2300/0864 , C12N5/0694 , C12Q1/24 , G01N15/0272 , G01N33/491 , G01N33/574 , G01N2015/0288
Abstract: The disclosure relates to an apparatus for segregating particles on the basis of their ability to flow through a stepped passageway. At least some of the particles are unable to pass through a narrower passageway bounded by a segregating step, resulting in segregation of the particles. The breadth of the leading edge of at least one step of the apparatus is significantly greater than the overall width of the passageway in which the step occurs, permitting high and rapid sample throughput. The apparatus and methods described herein can be used to segregate particles of a wide variety of types. By way of example, they can be used to segregate circulating tumor cells from a human blood sample.
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公开(公告)号：US09861981B2
公开(公告)日：2018-01-09
申请号：US14402945
申请日：2013-05-03
Inventor： Young-Ho Cho , Yoon-Ji Kim
IPC: B01L3/00
CPC classification number: B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2300/0645 , B01L2300/0816 , B01L2300/0851 , B01L2300/0864 , B01L2400/0481 , B01L2400/0655 , B01L2400/086
Abstract: A particle processing device includes a chamber including an input portion and an output portion and providing a space for flowing of a fluid having a particle, at least two deformable membrane structures sequentially arranged in the chamber and controlling a sectional area of a fluid path through which the fluid flows, and at least two membrane control lines respectively applying pressure to the deformable membrane structures.
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公开(公告)号：US20170361318A1
公开(公告)日：2017-12-21
申请号：US15695184
申请日：2017-09-05
Applicant: President and Fellows of Harvard College
Inventor： David A. Weitz , Darren Roy Link , Galder Cristobal-Azkarate , Zhengdong Cheng , Keunho Ahn
CPC classification number: B01L3/5027 , B01F5/0256 , B01F5/0646 , B01F5/0655 , B01F5/0682 , B01F5/0689 , B01F13/0069 , B01F13/0071 , B01F13/0074 , B01F13/0076 , B01J19/0093 , B01J2219/00783 , B01J2219/00862 , B01L3/0241 , B01L3/502761 , B01L3/502776 , B01L3/502784 , B01L3/502792 , B01L3/5088 , B01L2200/0636 , B01L2200/0652 , B01L2200/0673 , B01L2300/0864 , B01L2300/0867 , B01L2300/161 , B01L2400/0415 , B01L2400/0439 , B01L2400/0487 , C12Q2563/159 , C12Q2565/629 , G01N15/10 , G01N15/14 , G01N15/1459 , G01N15/1484 , G01N2015/1006 , G01N2015/1081 , G01N2015/149 , Y10S436/807 , Y10T436/118339 , Y10T436/2525 , Y10T436/2575
Abstract: Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. In some cases, the droplets may each have a substantially uniform number of entities therein. For example, 95% or more of the droplets may each contain the same number of entities of a particular species. In another aspect, the invention relates to systems and methods for dividing a fluidic droplet into two droplets, for example, through charge and/or dipole interactions with an electric field. The invention also relates to systems and methods for fusing droplets according to another aspect of the invention, for example, through charge and/or dipole interactions. In some cases, the fusion of the droplets may initiate or determine a reaction. In a related aspect of the invention, systems and methods for allowing fluid mixing within droplets to occur are also provided. In still another aspect, the invention relates to systems and methods for sorting droplets, e.g., by causing droplets to move to certain regions within a fluidic system. Examples include using electrical interactions (e.g., charges, dipoles, etc.) or mechanical systems (e.g., fluid displacement) to sort the droplets. In some cases, the fluidic droplets can be sorted at relatively high rates, e.g., at about 10 droplets per second or more. Another aspect of the invention provides the ability to determine droplets, or a component thereof, for example, using fluorescence and/or other optical techniques (e.g., microscopy), or electric sensing techniques such as dielectric sensing.
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