公开(公告)号：US20240327798A1

公开(公告)日：2024-10-03

申请号：US18380909

申请日：2023-10-17

申请人： DAVID MICHAELI ,  MENASHE MICHAELI

发明人： DAVID MICHAELI ,  MENASHE MICHAELI

IPC分类号： C12N5/09 ,  C12M1/00 ,  C12M1/26 ,  C12M1/42 ,  C12N13/00

CPC分类号： C12N5/0694 ,  C12M33/14 ,  C12M35/02 ,  C12M47/10 ,  C12N13/00

摘要： A system and method for preparing a cancer vaccine (and optionally purifying the blood) has a blood filtration system, controlled by a processing unit, for filtering exogenous blood plasma to isolate tumor cells, tumor stem cells and tumor breakdown products. The blood filtration system filter may include multiple layers having differently sized apertures to retain differently sized materials (from among tumor cells of different sizes, tumor stem cells and tumor breakdown products. A device directs electromagnetic radiation at the separated tumor cells, tumor stem cells and/or tumor breakdown products. The electromagnetic radiation may cause the separated tumor cells, separated tumor stem cells and/or separated tumor breakdown products (for example tumor protein such as DNA and/or tumor exosomes) to have a coagulated outer layer such as by degrading the outer surface. The electromagnetic radiation may have a UV wavelength. A conical coil improves flow rate uniformity. Tumor exosomes may be centrifuged.

公开(公告)号：US20240159764A1

公开(公告)日：2024-05-16

申请号：US18165425

申请日：2023-02-07

申请人： OneCell Diagnostics, Inc.

发明人： Jayant Jagannath Khandare ,  Ganesh Khutale ,  Atul Bharde ,  Snigdha Nadagouda ,  Gourishankar Rajshekhar Aland ,  Gowhar Shafi

IPC分类号： G01N33/574 ,  C12N5/09 ,  G01N33/552

CPC分类号： G01N33/57492 ,  C12N5/0694 ,  G01N33/552 ,  G01N2333/70589 ,  G01N2333/70596

摘要： This disclosure relates to adsorbent compositions and methods for selective capture, purification, and release of marker cells for cancer, more particularly, circulating rare cells exemplified by circulating tumour cells followed by their isolation as single cells. These adsorbent compositions include functionalized substrates linked to the ligands which bind non-covalently to a marker cell for cancer, through at least one linkage cleaved at controlled rate in the presence of specific stimuli, and also linked to ligands which bind non-covalently to leukocytes through non-cleavable linkages. The capture of marker cell for cancer and release may be monitored using microscopy. Methods for the capture and controlled release of these marker cells using these adsorbent compositions lead to isolation of single cells useful in diagnosis, prognosis and in the screening of therapeutic treatment of diseases, especially oncological diseases, genomics, single cell whole genome amplification, multi-omics, transcriptomics, proteomics, to identify genetic, genomic signatures, mutations.

公开(公告)号：US11773165B2

公开(公告)日：2023-10-03

申请号：US17736874

申请日：2022-05-04

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Ravindra Majeti ,  Irving L. Weissman

IPC分类号： A61P35/02 ,  C07K16/28 ,  C07K16/30 ,  C12Q1/6886 ,  G01N33/50 ,  G01N33/574 ,  C12N5/00 ,  C12N5/09 ,  A61K39/00

CPC分类号： C07K16/2803 ,  A61P35/02 ,  C07K16/28 ,  C07K16/2896 ,  C07K16/30 ,  C12N5/0093 ,  C12N5/0694 ,  C12Q1/6886 ,  G01N33/5011 ,  G01N33/57426 ,  A61K2039/505 ,  A61K2039/507 ,  C07K2317/24 ,  C07K2317/73 ,  C07K2317/75 ,  C07K2317/76 ,  C12Q2600/112 ,  C12Q2600/136 ,  G01N2500/04

摘要： Markers of acute myeloid leukemia stem cells (AMLSC) are identified. The markers are differentially expressed in comparison with normal counterpart cells, and are useful as diagnostic and therapeutic targets.

公开(公告)号：US11667894B2

公开(公告)日：2023-06-06

申请号：US16921007

申请日：2020-07-06

申请人： Yao Tang

发明人： Yao Tang

IPC分类号： C12N5/09

CPC分类号： C12N5/0694 ,  C12N5/0693 ,  C12N2500/84 ,  C12N2503/02 ,  C12N2509/00 ,  C12N2509/10 ,  C12N2513/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

摘要： The present invention provides methods for culturing primary cells and tissues from a subject in the presence of the subject's own serum, ascites or pleural effusion fluid. Methods of treating cancer, and screening for the effectiveness or toxicity of drugs are also provided herein.

公开(公告)号：US20190234874A1

公开(公告)日：2019-08-01

申请号：US16319099

申请日：2017-07-19

申请人： ALTIUS INSTITUTE FOR BIOMEDICAL SCIENCES ,  UNIVERSITY OF WASHINGTON

发明人： John Stamatoyannopoulos ,  Shreeram AKILESH

IPC分类号： G01N21/64 ,  G01N21/78 ,  C12P19/34 ,  A01N1/02 ,  C12N5/09 ,  C12N5/078 ,  G01N1/30

CPC分类号： G01N21/64 ,  A01N1/0221 ,  C12N5/0634 ,  C12N5/0694 ,  C12P19/34 ,  C12Q1/6841 ,  C40B30/04 ,  C40B40/06 ,  G01N1/30 ,  G01N21/6458 ,  G01N21/78 ,  G01N33/56966 ,  G01N33/574 ,  G01N33/582 ,  G01N2015/0038 ,  C12Q2521/301 ,  C12Q2563/107

摘要： Disclosed herein are methods of detecting a regulatory element, determining the localization of a regulatory element, and/or measuring the activity of a regulatory element.

公开(公告)号：US20190216907A1

公开(公告)日：2019-07-18

申请号：US16367213

申请日：2019-03-27

申请人： BioVentures, LLC

发明人： Thomas Kieber-Emmons ,  Anastas Pashov ,  Behjatolah Karbassi

IPC分类号： A61K39/00 ,  A61K31/704 ,  A61K35/15 ,  A61K35/17 ,  C07K16/32 ,  C07K7/08 ,  A61K31/7024 ,  A61K31/138 ,  A61K31/337 ,  A61K31/475 ,  A61K31/675 ,  A61K45/06 ,  C12N5/09 ,  C07K14/47 ,  C07K16/18 ,  C12N5/0783 ,  A61K39/39

CPC分类号： A61K39/0005 ,  A61K31/138 ,  A61K31/337 ,  A61K31/475 ,  A61K31/675 ,  A61K31/7024 ,  A61K31/704 ,  A61K35/15 ,  A61K35/17 ,  A61K39/0011 ,  A61K39/39 ,  A61K45/06 ,  A61K2039/507 ,  A61K2039/5154 ,  A61K2039/5158 ,  A61K2039/55 ,  A61K2039/55566 ,  A61K2039/572 ,  A61K2039/575 ,  A61K2039/585 ,  A61K2039/605 ,  C07K7/08 ,  C07K14/4748 ,  C07K16/18 ,  C07K16/32 ,  C07K2317/73 ,  C07K2319/00 ,  C07K2319/33 ,  C12N5/0646 ,  C12N5/0694 ,  A61K2300/00 ,  A61K31/34 ,  A61K39/00

摘要： Compositions useful to induce anti-TACA immune response and kits comprising the same and methods of using the compositions to treat and prevent cancer are disclosed. Isolated human antibodies which bind to a carbohydrate mimetic peptide and methods of making the same and using the same to treat and prevent cancer are disclosed. Isolated human NK cells useful to kill tumor cells, and isolated human dendritic cells, and methods of making the same and using the same to treat and prevent cancer are disclosed.

公开(公告)号：US20190046572A1

公开(公告)日：2019-02-14

申请号：US16156996

申请日：2018-10-10

申请人： Fred Hutchinson Cancer Research Center

发明人： Matthias Stephan

IPC分类号： A61K35/17 ,  C12N5/09 ,  A01K67/027 ,  C07K16/28 ,  A61K9/14 ,  A61K39/44 ,  A61K45/06 ,  A61K9/00

CPC分类号： A61K35/17 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/0331 ,  A61K9/0024 ,  A61K9/146 ,  A61K39/44 ,  A61K45/06 ,  C07K16/2809 ,  C07K16/2818 ,  C07K16/2878 ,  C12N5/0694 ,  C12N2510/00 ,  C12N2533/54 ,  C12N2533/74

摘要： The present disclosure provides compositions and methods for the delivery of immune cells to treat un-resectable or non-resected tumor cells and tumor relapse. The compositions comprise (i) a structure comprising an injectable polymer or scaffold comprising pores; (ii) lymphocytes disposed within the structure, (iii) at least one lymphocyte-adhesion moiety associated with the structure; and (iv) at least one lymphocyte-activating moiety associated with the structure, and optionally an immune stimulant.

公开(公告)号：US20180255752A1

公开(公告)日：2018-09-13

申请号：US15770811

申请日：2016-10-26

申请人： PÉCSI TUDOMÁNYEGYETEM

发明人： Péter BALOGH ,  Dávid ERNSZT ,  Tamás KOVÁCS

IPC分类号： A01K67/027 ,  C12N5/09

CPC分类号： A01K67/0271 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/0331 ,  A01K2267/0381 ,  C12N5/0694

摘要： The invention relates to a new, spontaneous mouse lymphoma cell line displaying CD19, B220, MHC II, surface IgG2a/kappa chain and MAC-1, which is negative for CD5, animal models of B-cell lymphoma based on said cell line and methods for assessing lymphoma propagation and lymphoma expansion based on said cell line.

公开(公告)号：US20170239294A1

公开(公告)日：2017-08-24

申请号：US15519323

申请日：2015-10-15

申请人： THE CHILDREN'S HOSPITAL OF PHILADELPHIA

发明人： Andrei Thomas-Tikhonenko ,  Stephan Grupp ,  John Maris ,  David Barrett ,  Elena Sotillo-Pineiro

IPC分类号： A61K35/17 ,  A61K45/06 ,  A61K31/506

CPC分类号： A61K35/17 ,  A61K31/506 ,  A61K45/06 ,  C12N5/0694 ,  A61K2300/00

摘要： Compositions and methods for inhibiting, treating, and/or preventing a B-cell neoplasm are provided.

公开(公告)号：US20170160263A1

公开(公告)日：2017-06-08

申请号：US15434249

申请日：2017-02-16

申请人： ANGLE North America, Inc.

发明人： Georgi HVICHIA

IPC分类号： G01N33/49 ,  C12N5/09 ,  B01L3/00

CPC分类号： B01L3/502753 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  C12N5/0694 ,  C12Q1/24 ,  G01N15/0272 ,  G01N33/491 ,  G01N33/574 ,  G01N2015/0288

摘要： The disclosure relates to an apparatus for segregating particles on the basis of their ability to flow through a stepped passageway. At least some of the particles are unable to pass through a narrower passageway bounded by a segregating step, resulting in segregation of the particles. The breadth of the leading edge of at least one step of the apparatus is significantly greater than the overall width of the passageway in which the step occurs, permitting high and rapid sample throughput. The apparatus and methods described herein can be used to segregate particles of a wide variety of types. By way of example, they can be used to segregate circulating tumor cells from a human blood sample.