公开(公告)号：US12129506B2

公开(公告)日：2024-10-29

申请号：US17809750

申请日：2022-06-29

申请人： CARGILL, INCORPORATED

发明人： Michael D. Lynch ,  Michael Tai Man Louie ,  Shelley Copley ,  Eileen Colie Spindler ,  Brittany Robinson ,  Matthew Lipscomb ,  Tanya Lipscomb-Warnecke ,  Hans H. Liao ,  David Hogsett ,  Ron Evans

IPC分类号： C12P7/6409 ,  C12N1/20 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88

CPC分类号： C12P7/6409 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12Y101/01035 ,  C12Y103/01093 ,  C12Y203/01194 ,  C12Y402/01059 ,  C12Y203/01075 ,  C12Y301/02

摘要： This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.

公开(公告)号：US12129475B2

公开(公告)日：2024-10-29

申请号：US16484200

申请日：2018-02-08

申请人： YEDA RESEARCH AND DEVELOPMENT CO. LTD. ,  Yissum Research Development Company of The Hebrew University of Jerusalem Ltd.

发明人： Asaph Aharoni ,  Prashant Sonawane ,  Pablo D. Cardenas ,  Burdman Saul

IPC分类号： C12N15/82 ,  C12N9/02 ,  C12N9/04

CPC分类号： C12N15/8279 ,  C12N9/0006 ,  C12N9/0071 ,  C12N15/8218 ,  C12N15/8243

摘要： Disclosed herein are genetically modified plants having altered biological activity of 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), or 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plants have an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Further disclosed herein are genetically modified plants having altered expression of a gene encoding a 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), or a 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plant has an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Methods of producing these genetically modified plants are also disclosed.

公开(公告)号：US12116610B2

公开(公告)日：2024-10-15

申请号：US17585119

申请日：2022-01-26

申请人： The University of North Carolina at Chapel Hill

发明人： Koji Sode ,  Junko Shimazaki

IPC分类号： C12N9/04 ,  C12Q1/00 ,  C12Q1/26

CPC分类号： C12Q1/005 ,  C12N9/0006 ,  C12Q1/26 ,  C12Y101/03021

摘要： Compositions, devices, kits and methods are disclosed for assaying triglyceride with a glycerol 3-phosphate oxidase mutant. The glycerol 3-phosphate oxidase mutant has reduced oxidase activity while substantially maintaining, or increasing, its dehydrogenase activity, compared to the wild-type glycerol 3-phosphate oxidase.

公开(公告)号：US12110527B2

公开(公告)日：2024-10-08

申请号：US17292092

申请日：2019-11-21

申请人： UNIVERSITEIT GENT ,  AMPHISTAR

发明人： Sophie Roelants ,  Lisa Van Renterghem ,  Wim Soetaert ,  Jelle Remmery

IPC分类号： C12P19/44 ,  C12N9/02 ,  C12N9/04 ,  C12N15/01 ,  C12P1/02 ,  C12P7/6436

CPC分类号： C12P19/44 ,  C12N9/0006 ,  C12N9/0071 ,  C12N15/01 ,  C12Y101/0302 ,  C12Y114/14001

摘要： The present invention relates to the field of microbial production of novel biosurfactants. More specifically, the present invention discloses the usage of a fungal strain such as the yeast Starmerella bombicola having a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase for producing high amounts of so-called “symmetrical bolaform sophorosides” where both sophorose moieties are attached through a terminal glycosidic linkage to the hydrophobic linker. In addition, the present invention further discloses that the latter yeast can also be used to produce alkyl sophorosides and symmetrical bolaform glucosides.

公开(公告)号：US20240327880A1

公开(公告)日：2024-10-03

申请号：US18437245

申请日：2024-02-09

申请人： Jiangnan University

发明人： Jingwen Zhou ,  Jian Chen ,  Lian Wang ,  Weizhu Zeng

IPC分类号： C12P7/62 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/70

CPC分类号： C12P7/62 ,  C12N9/0006 ,  C12N9/0071 ,  C12N9/10 ,  C12N9/1085 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70 ,  C12Y101/01006 ,  C12Y101/01282 ,  C12Y114/14009 ,  C12Y205/01054 ,  C12Y402/03004 ,  C12Y403/01023 ,  C12Y504/99005 ,  C12Y602/01012

摘要： The present disclosure provides a recombinant Escherichia coli for producing chlorogenic acid and application thereof. In the present disclosure, tyrosine ammonia-lyase FjTAL derived from Flavobacterium johnsoniae, hpaBC derived from E. coli, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase mutant aroGfbr, chorismate mutase tyrC derived from Zymomonas mobilis, quinic acid/shikimate-5 dehydrogenase ydiB derived from E. coli, hydroxycinnamoyl CoA:quinic acid transferase NtHQT derived from Nicotiana tabacum, and 4-coumarate:CoA ligase At4CL1 derived from Arabidopsis thaliana are expressed in the recombinant E. coli, thereby constructing a chlorogenic acid biosynthesis pathway in E. coli. Then, the aroB gene and gldA gene derived from E. coli are overexpressed, and an endogenous gene menI is knocked out from the recombinant E. coli. The recombinant strain produced chlorogenic acid by fermentation at a titer of up to 638.2 mg/L in a shake flask or at a titer of 2.8 g/L in a 5-L fermenter.

公开(公告)号：US12104191B2

公开(公告)日：2024-10-01

申请号：US17823853

申请日：2022-08-31

申请人： ZHEJIANG UNIVERSITY OF TECHNOLOGY

发明人： Yaping Xue ,  Shuping Zou ,  Zhentao Jiang ,  Jianmiao Xu ,  Feng Cheng ,  Yuguo Zheng

IPC分类号： C12P13/00 ,  C07K14/245 ,  C12N1/21 ,  C12N9/00 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N15/10 ,  C12N15/52 ,  C12N15/70

CPC分类号： C12P13/001 ,  C07K14/245 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/1077 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/93 ,  C12N15/1082 ,  C12N15/52 ,  C12N15/70 ,  C12Y101/01119 ,  C12Y104/01002 ,  C12Y204/02011 ,  C12Y207/01086 ,  C12Y207/07018 ,  C12Y603/01005 ,  C12N2800/101

摘要： A cofactor self-sufficient Escherichia coli and its construction method and application in the synthesis of L-glufosinate are provided. The present invention expresses a NADH kinase and key enzymes of the cofactor synthesis pathway in E. coli, and knocks out the genes of enzymes that catabolizes cofactor, and with the addition of co-metabolic intermediates during cell incubation, the intracellular NADP(H) concentration is increased by at least 50% and the catalytic activity of glufosinate dehydrogenase by 2-fold, resulting in a significant increase in the spatiotemporal yield of the glufosinate synthesis reaction.

公开(公告)号：US12104160B2

公开(公告)日：2024-10-01

申请号：US17307850

申请日：2021-05-04

申请人： ZYMOCHEM, INC.

发明人： Harshal Chokhawala

IPC分类号： C12P7/42 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/24 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/00 ,  C12P17/08 ,  C12P17/10

CPC分类号： C12N15/52 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12P17/10 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01035 ,  C12Y101/01078 ,  C12Y101/01268 ,  C12Y101/01269 ,  C12Y102/01003 ,  C12Y102/01005 ,  C12Y102/01022 ,  C12Y102/01024 ,  C12Y102/01026 ,  C12Y102/01048 ,  C12Y102/01063 ,  C12Y102/07005 ,  C12Y102/99006 ,  C12Y103/01044 ,  C12Y103/01045 ,  C12Y103/01086 ,  C12Y104/01 ,  C12Y203/01001 ,  C12Y203/01032 ,  C12Y203/01035 ,  C12Y203/01057 ,  C12Y206/01008 ,  C12Y206/01009 ,  C12Y206/01036 ,  C12Y206/01043 ,  C12Y206/01048 ,  C12Y206/01076 ,  C12Y206/01082 ,  C12Y207/01031 ,  C12Y207/01165 ,  C12Y208/03 ,  C12Y301/03 ,  C12Y301/03002 ,  C12Y301/03008 ,  C12Y301/03019 ,  C12Y301/0302 ,  C12Y302/01 ,  C12Y401/01001 ,  C12Y401/02 ,  C12Y401/02014 ,  C12Y401/0202 ,  C12Y401/02021 ,  C12Y401/03016 ,  C12Y401/03017 ,  C12Y402/01002 ,  C12Y402/01003 ,  C12Y402/01028 ,  C12Y402/0103 ,  C12Y402/01079 ,  C12Y402/0112 ,  C12Y602/01 ,  Y02E50/30

摘要： Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a CN aldehyde and pyruvate to a CN+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the CN+3 β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

公开(公告)号：US12098394B2

公开(公告)日：2024-09-24

申请号：US17290749

申请日：2018-11-05

申请人： ASYMCHEM LABORATORIES (TIANJIN) CO., LTD.

发明人： Hao Hong ,  Gage James ,  Jiangping Lu ,  Xuecheng Jiao ,  Na Zhang ,  Kejian Zhang ,  Rui Li ,  Yu Zhang ,  Yiming Yang

IPC分类号： C12N9/04 ,  C12P11/00

CPC分类号： C12N9/0006 ,  C12P11/00

摘要： An amino acid sequence of the monooxygenase mutant is obtained by mutation of an amino acid sequence shown in SEQ ID NO: 1, and the mutation at least includes one of the following mutation sites: 45-th site, 95-th site, 106-th site, 108-th site, 114-th site, 186-th site, 190-th site, 191-th site, 249-th site, 257-th site, 393-th site, 436-th site, 499-th site, 500-th site, 501-th site, 503-th site, 504-th site, 559-th site, and 560-th site.

公开(公告)号：US20240279694A1

公开(公告)日：2024-08-22

申请号：US18582231

申请日：2024-02-20

申请人： BIOMEDICAN, INC.

发明人： Maxim Mikheev ,  Difeng Gao

IPC分类号： C12P17/06 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88

CPC分类号： C12P17/06 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/93 ,  C12Y101/01034 ,  C12Y121/03007 ,  C12Y121/03008 ,  C12Y203/0102 ,  C12Y203/01206 ,  C12Y404/01026 ,  C12Y604/01002

摘要： The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

公开(公告)号：US20240263130A1

公开(公告)日：2024-08-08

申请号：US18031556

申请日：2022-06-28

申请人： SHENZHEN BLUEPHA BIOSCIENCES CO., LTD.

发明人： Jin YIN ,  Yu WANG ,  Jiajia LI ,  Jie HOU ,  Liang ZOU ,  Zixian CHEN

IPC分类号： C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/74 ,  C12N15/90 ,  C12P7/625 ,  C12R1/01

CPC分类号： C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/74 ,  C12N15/90 ,  C12P7/625 ,  C12N2510/00 ,  C12R2001/01 ,  C12Y101/01036 ,  C12Y402/01119

摘要： The present invention relates to the technical field of microorganisms, and specifically to engineered microorganisms expressing acetoacetyl-CoA reductase variants and methods for increasing the proportion of 3-hydroxyhexanoic acid in PHA. The acetoacetyl-CoA reductase variants and their coding genes provided by the present invention can significantly increase the proportion of 3-hydroxyhexanoic acid in PHA produced by strains; the proportion of 3-hydroxyhexanoic acid in PHA produced by the engineered Ralstonia eutropha constructed utilizing the acetoacetyl-CoA reductase variants and their coding genes provided by the present invention is significantly increased, which provides new genes and strain resources for strains producing poly(3-hydroxybutyrate-co-3-10 the development of engineered hydroxyhexanoate) with high proportion of 3-hydroxyhexanoic acid.