公开(公告)号：US20230159978A1

公开(公告)日：2023-05-25

申请号：US17930462

申请日：2022-09-08

申请人： ExxonMobil Technology and Engineering Company

发明人： Xiaozhou Zhang ,  Mohor Chatterjee

IPC分类号： C12Q1/28 ,  C12P7/04 ,  C12P7/24 ,  C12P3/00 ,  C12N9/04 ,  C12N9/88

CPC分类号： C12Q1/28 ,  C12P7/04 ,  C12P7/24 ,  C12P3/00 ,  C12N9/0006 ,  C12Y101/0302 ,  C12N9/88 ,  C12Y402/01053

摘要： A variety of methods and systems for screening biocatalysts are disclosed, including, in one embodiment, a screening method for identifying engineered biocatalysts, including reacting an olefin with water in the presence of an engineered biocatalyst to produce at least a fatty alcohol having from 4 carbons to 24 carbons; reacting at least a portion of the fatty alcohol with oxygen in the present of a fatty alcohol oxidase to produce a fatty aldehyde and hydrogen peroxide, the fatty aldehyde having from 4 carbons to 24 carbons; and measuring activity of the engineered biocatalyst.

公开(公告)号：US12110527B2

公开(公告)日：2024-10-08

申请号：US17292092

申请日：2019-11-21

申请人： UNIVERSITEIT GENT ,  AMPHISTAR

发明人： Sophie Roelants ,  Lisa Van Renterghem ,  Wim Soetaert ,  Jelle Remmery

IPC分类号： C12P19/44 ,  C12N9/02 ,  C12N9/04 ,  C12N15/01 ,  C12P1/02 ,  C12P7/6436

CPC分类号： C12P19/44 ,  C12N9/0006 ,  C12N9/0071 ,  C12N15/01 ,  C12Y101/0302 ,  C12Y114/14001

摘要： The present invention relates to the field of microbial production of novel biosurfactants. More specifically, the present invention discloses the usage of a fungal strain such as the yeast Starmerella bombicola having a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase for producing high amounts of so-called “symmetrical bolaform sophorosides” where both sophorose moieties are attached through a terminal glycosidic linkage to the hydrophobic linker. In addition, the present invention further discloses that the latter yeast can also be used to produce alkyl sophorosides and symmetrical bolaform glucosides.

公开(公告)号：US20230175032A1

公开(公告)日：2023-06-08

申请号：US17921963

申请日：2021-05-12

申请人： AMPHISTAR ,  UNIVERSITEIT GENT

发明人： Wim SOETAERT ,  Sophie ROELANTS ,  Christian STEVENS ,  Elisabeth DELBEKE ,  Goedele LUYTEN ,  Melike PALA ,  Jelle REMMERY

IPC分类号： C12P19/44 ,  C12N9/04 ,  C12N9/10 ,  C12P7/6409

CPC分类号： C12P19/44 ,  C12N9/0006 ,  C12N9/1048 ,  C12P7/6409 ,  C12Y101/0302 ,  C12Y114/14 ,  C12P7/18

摘要： The present invention relates to the field of production of novel biosurfactants. More specifically, the present invention relates to the efficient generation of short chained on-glycosides with less than 10%, preferably less than 1%, ω-1 glycosides using a fungal strain such as the yeast Starmerella bombicola having a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase to produce high amounts of so-called unsaturated (symmetrical) α,ω-bola glycosides free from contaminating α,ω-1 bola glycosides, and subjecting said unsaturated (symmetrical) α,ω-bola glycosides to conditions inducing the breaking of the present double bond(s) such as for example through ozonolysis performed in water. More specifically, the present invention discloses the generation of (acetylated) C9:0 ω-sophoroside aldehydes, C9:0 ω-glucoside aldehydes, C9:0 ω-glucolipids, C9:0 ω-sophorolipids, C9:0 ω-sophoroside alcohols and C9:0 ω-glucoside alcohols and their further derivatives. The present invention also discloses methods to produce alkyl sophorosides in increased ratios.

公开(公告)号：US11969246B2

公开(公告)日：2024-04-30

申请号：US18319085

申请日：2023-05-17

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： Samson Chen ,  Axel Scherer ,  Dvin I. Adalian ,  Peter Petillo ,  Muhammad Musab Jilani ,  Xiomara L. Madero ,  Deepan Kishore Kumar

IPC分类号： C12N11/14 ,  A61B5/145 ,  A61B5/1459 ,  A61B5/1486 ,  C12N9/02 ,  C12N11/02 ,  G01N27/327

CPC分类号： A61B5/14865 ,  A61B5/14532 ,  A61B5/1459 ,  C12N9/0004 ,  C12N11/02 ,  C12N11/14 ,  G01N27/3272 ,  C12Y101/01001 ,  C12Y101/03003 ,  C12Y101/03005 ,  C12Y101/03007 ,  C12Y101/03008 ,  C12Y101/0301 ,  C12Y101/03011 ,  C12Y101/03012 ,  C12Y101/03015 ,  C12Y101/03016 ,  C12Y101/03018 ,  C12Y101/03019 ,  C12Y101/0302 ,  C12Y101/03023 ,  C12Y101/03027 ,  C12Y101/03029 ,  C12Y101/0303 ,  C12Y101/03037 ,  C12Y101/03038 ,  C12Y101/0304 ,  C12Y101/03041

摘要： A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.

公开(公告)号：US20160304913A1

公开(公告)日：2016-10-20

申请号：US15103191

申请日：2014-12-10

申请人： TECHNISCHE UNIVERSITÄT DRESDEN

发明人： Michael GATTER ,  Falk MATTHAUS ,  Gerold BARTH

IPC分类号： C12P7/64 ,  C12N9/02 ,  C12N9/90 ,  C12P7/44 ,  C12N9/04

CPC分类号： C12P7/6409 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/90 ,  C12P7/44 ,  C12P7/6427 ,  C12Y101/0302 ,  C12Y103/03006 ,  C12Y502/01008

摘要： The invention relates to various new yeast strains of the type Yarrowia lipolytica as well as relevant methods for the biocatalytic preparation of ω-hydroxy fatty acids or dicarboxylic acids with the aid of these strains, whereby the formation of ω-hydroxy fatty acids or dicarboxylic acids is advantageously increased.

摘要翻译： 本发明涉及解脂耶氏酵母属的各种新的酵母菌株以及借助于这些菌株生物催化制备ω-羟基脂肪酸或二羧酸的相关方法，从而形成ω-羟基脂肪酸或二羧酸 有利地增加。

公开(公告)号：US20240260865A1

公开(公告)日：2024-08-08

申请号：US18623971

申请日：2024-04-01

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： Samson CHEN ,  Axel SCHERER ,  Dvin I. ADALIAN ,  Peter PETILLO ,  Muhammad Musab JILANI ,  Xiomara L. MADERO ,  Deepan KISHORE KUMAR

IPC分类号： A61B5/1486 ,  A61B5/145 ,  A61B5/1459 ,  C12N9/02 ,  C12N11/02 ,  C12N11/14 ,  G01N27/327

CPC分类号： A61B5/14865 ,  A61B5/14532 ,  A61B5/1459 ,  C12N9/0004 ,  C12N11/02 ,  C12N11/14 ,  G01N27/3272 ,  C12Y101/01001 ,  C12Y101/03003 ,  C12Y101/03005 ,  C12Y101/03007 ,  C12Y101/03008 ,  C12Y101/0301 ,  C12Y101/03011 ,  C12Y101/03012 ,  C12Y101/03015 ,  C12Y101/03016 ,  C12Y101/03018 ,  C12Y101/03019 ,  C12Y101/0302 ,  C12Y101/03023 ,  C12Y101/03027 ,  C12Y101/03029 ,  C12Y101/0303 ,  C12Y101/03037 ,  C12Y101/03038 ,  C12Y101/0304 ,  C12Y101/03041

摘要： A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.

公开(公告)号：US20240110208A1

公开(公告)日：2024-04-04

申请号：US18189272

申请日：2023-03-24

申请人： NATIONAL TSING HUA UNIVERSITY ,  Chang Chun Plastics Co., Ltd. ,  Chang Chun Petrochemical Co., LTD. ,  DAIREN CHEMICAL CORP.

发明人： Yu-Chen HU ,  Nam Ngoc PHAM ,  June-Yen CHOU ,  Hsing-Yun WANG ,  Vincent Jianan LIU

IPC分类号： C12P7/6409 ,  C12N15/52 ,  C12N15/90

CPC分类号： C12P7/6409 ,  C12N15/52 ,  C12N15/905 ,  C12Y101/01 ,  C12Y101/0302 ,  C12Y114/14 ,  C12Y207/01086

摘要： A gene editing system of Candida viswanathii includes a Candida viswanathii, a first gene editing fragment and a second gene editing fragment. The first gene editing fragment successively includes a first homology arm and a screening gene. The second gene editing fragment is connected to a C-terminus of the first gene editing fragment and includes a second homology arm, a Cas9 expression cassette and a sgRNA cassette. The Cas9 expression cassette successively includes a Cas9 promoter, a Cas9 gene and three nuclear localization sequences. The sgRNA cassette successively includes a sgRNA promoter, a first ribozyme, a targeting sequence, a scaffold and a second ribozyme. The first gene editing fragment and the second gene editing fragment are constructed as a linear fragment for gene editing of a chromosome of the Candida viswanathii.

公开(公告)号：US20230363672A1

公开(公告)日：2023-11-16

申请号：US18319085

申请日：2023-05-17

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： Samson CHEN ,  Axel SCHERER ,  Dvin I. ADALIAN ,  Peter PETILLO ,  Muhammad Musab JILANI ,  Xiomara L. MADERO ,  Deepan KISHORE KUMAR

IPC分类号： A61B5/1486 ,  C12N11/14 ,  A61B5/145 ,  A61B5/1459 ,  G01N27/327 ,  C12N9/02 ,  C12N11/02

CPC分类号： A61B5/14865 ,  A61B5/14532 ,  A61B5/1459 ,  C12N9/0004 ,  C12N11/02 ,  C12N11/14 ,  G01N27/3272 ,  C12Y101/01001 ,  C12Y101/03003 ,  C12Y101/03005 ,  C12Y101/03007 ,  C12Y101/03008 ,  C12Y101/0301 ,  C12Y101/03011 ,  C12Y101/03012 ,  C12Y101/03015 ,  C12Y101/03016 ,  C12Y101/03018 ,  C12Y101/03019 ,  C12Y101/0302 ,  C12Y101/03023 ,  C12Y101/03027 ,  C12Y101/03029 ,  C12Y101/0303 ,  C12Y101/03037 ,  C12Y101/03038 ,  C12Y101/0304 ,  C12Y101/03041

摘要： A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.

公开(公告)号：US11690544B2

公开(公告)日：2023-07-04

申请号：US17314930

申请日：2021-05-07

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： Samson Chen ,  Axel Scherer ,  Dvin Adalian ,  Peter Petillo ,  Muhammad Musab Jilani ,  Xiomara L. Madero ,  Deepan Kishore Kumar

IPC分类号： A61B5/145 ,  C12N11/02 ,  A61B5/1486 ,  C12N9/02 ,  A61B5/1459 ,  G01N27/327 ,  C12N11/14

CPC分类号： A61B5/14865 ,  A61B5/1459 ,  A61B5/14532 ,  C12N9/0004 ,  C12N11/02 ,  C12N11/14 ,  G01N27/3272 ,  C12Y101/01001 ,  C12Y101/0301 ,  C12Y101/0302 ,  C12Y101/03003 ,  C12Y101/0303 ,  C12Y101/0304 ,  C12Y101/03005 ,  C12Y101/03007 ,  C12Y101/03008 ,  C12Y101/03011 ,  C12Y101/03012 ,  C12Y101/03015 ,  C12Y101/03016 ,  C12Y101/03018 ,  C12Y101/03019 ,  C12Y101/03023 ,  C12Y101/03027 ,  C12Y101/03029 ,  C12Y101/03037 ,  C12Y101/03038 ,  C12Y101/03041

摘要： A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.

公开(公告)号：US20170145448A1

公开(公告)日：2017-05-25

申请号：US15359932

申请日：2016-11-23

申请人： Evonik Degussa GmbH

发明人： Steffen SCHAFFER ,  Thomas Haas ,  Wilhelm Bruegging ,  Ralf Meier

IPC分类号： C12P7/62 ,  C12N9/04 ,  C12N9/10 ,  C12N9/02

CPC分类号： C12P7/62 ,  C12N9/0006 ,  C12N9/0077 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/52 ,  C12P7/02 ,  C12P7/24 ,  C12P7/6436 ,  C12P7/649 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/0302 ,  C12Y114/15003 ,  C12Y203/01075 ,  C12Y203/01084 ,  C12Y206/01 ,  Y02E50/13

摘要： There is provided a microbial cell for producing at least one omega-functionalized carboxylic acid ester from at least one alkane, wherein the cell is genetically modified to increase the expression relative to the wild type cell of (i) Enzyme E1 capable of converting the alkane to the corresponding 1-alkanol; (ii) Enzyme E2 capable of converting the 1-alkanol of (i) to the corresponding 1-alkanal; (iii) Enzyme E3 capable of converting the 1-alkanal of (ii) to the corresponding alkanoic acid; (iv) Enzyme E4 capable of converting the alkanoic acid of (iii) to the corresponding alkanoic acid ester; and (iv) Enzyme E5 capable of converting the alkanoic acid ester of (iv) to the corresponding omega-hydroxy-alkanoic acid ester, and wherein the cell does not comprise a genetic modification that increases the expression relative to the wild type cell of at least one of the following enzymes E20-E24 selected from the group consisting of: E20 Acyl-ACP thioesterase, of EC 3.1.2.14 or EC 3.1.2.22, E21 Acyl-CoA thioesterase, of EC 3.1.2.2, EC 3.1.2.18, EC 3.1.2.19, EC 3.1.2.20 or EC 3.1.2.22, E22 Acyl-CoA:ACP transacylase, E23 Polyketide synthase, and E24 Hexanoic acid synthase