公开(公告)号：US11109784B2

公开(公告)日：2021-09-07

申请号：US16191357

申请日：2018-11-14

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Dvin Adalian ,  Samson Chen ,  Muhammad M. Jilani ,  Axel Scherer ,  Xiomara Linnette Madero ,  Richard Smith

IPC: A61B5/1486 ,  A61B5/145 ,  A61B5/1473

Abstract: Measurement of target analytes is carried out with an enzyme-based sensor. The enzyme hydrogel is protected by a porous layer of a metallic material. The size of the pores is small enough to prevent degradation of the enzyme layer caused by the immune system of an organism, but large enough to allow transfer of molecules that participate in the electrochemical reaction allowing the enzyme to detect the target analytes.

公开(公告)号：US11690544B2

公开(公告)日：2023-07-04

申请号：US17314930

申请日：2021-05-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Samson Chen ,  Axel Scherer ,  Dvin Adalian ,  Peter Petillo ,  Muhammad Musab Jilani ,  Xiomara L. Madero ,  Deepan Kishore Kumar

IPC: A61B5/145 ,  C12N11/02 ,  A61B5/1486 ,  C12N9/02 ,  A61B5/1459 ,  G01N27/327 ,  C12N11/14

CPC classification number: A61B5/14865 ,  A61B5/1459 ,  A61B5/14532 ,  C12N9/0004 ,  C12N11/02 ,  C12N11/14 ,  G01N27/3272 ,  C12Y101/01001 ,  C12Y101/0301 ,  C12Y101/0302 ,  C12Y101/03003 ,  C12Y101/0303 ,  C12Y101/0304 ,  C12Y101/03005 ,  C12Y101/03007 ,  C12Y101/03008 ,  C12Y101/03011 ,  C12Y101/03012 ,  C12Y101/03015 ,  C12Y101/03016 ,  C12Y101/03018 ,  C12Y101/03019 ,  C12Y101/03023 ,  C12Y101/03027 ,  C12Y101/03029 ,  C12Y101/03037 ,  C12Y101/03038 ,  C12Y101/03041

Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.

公开(公告)号：US11026610B2

公开(公告)日：2021-06-08

申请号：US16144079

申请日：2018-09-27

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Samson Chen ,  Axel Scherer ,  Dvin Adalian ,  Peter Petillo ,  Muhammad Musab Jilani ,  Xiomara L. Madero ,  Deepan Kishore Kumar

IPC: C12N9/02 ,  C12N9/04 ,  C12N11/14 ,  A61B5/1486 ,  C12N11/02 ,  A61B5/145 ,  A61B5/1459 ,  G01N27/327

Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.