公开(公告)号：US11959856B2

公开(公告)日：2024-04-16

申请号：US15701014

申请日：2017-09-11

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. Malik ,  Xiomara Linnette Madero ,  Erika F. Garcia ,  Sheel Mukesh Shah ,  Axel Scherer

IPC: G01N21/64 ,  C12Q1/6851

CPC classification number: G01N21/6486 ,  C12Q1/6851 ,  C12Q1/6851 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2565/629

Abstract: Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.

公开(公告)号：US09284520B2

公开(公告)日：2016-03-15

申请号：US14070454

申请日：2013-11-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. Malik ,  Erika F. Garcia ,  Xiomara Linnette Madero ,  Axel Scherer

IPC: G01N21/00 ,  C12M1/40 ,  B01L3/00 ,  B01L9/00 ,  B01L7/00

CPC classification number: C12M21/18 ,  B01L3/0275 ,  B01L3/502707 ,  B01L3/502715 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/023 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1827 ,  B01L2400/0478

Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.

Abstract translation: 公开了用于生物样品制备和分析的方法和装置。 装置可以具有容器的线性或圆形布置，其具有连接结构，例如杆或盘。 容器之间的流体通道允许执行不同的样品制备技术，如裂解，洗涤和洗脱。 可以将不同的功能元件，例如研磨机或混合器附接到容器。

公开(公告)号：US09791372B2

公开(公告)日：2017-10-17

申请号：US13958479

申请日：2013-08-02

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. Malik ,  Xiomara Linnette Madero ,  Erika F. Garcia ,  Sheel Mukesh Shah ,  Axel Scherer

IPC: G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6486 ,  C12Q1/6851 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2565/629

Abstract: Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.

公开(公告)号：US09090890B2

公开(公告)日：2015-07-28

申请号：US14070460

申请日：2013-11-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R Malik ,  Erika F Garcia ,  Xiomara Linnette Madero ,  Axel Scherer

IPC: G01N21/75 ,  C12N15/10 ,  F17D3/00

CPC classification number: C12N15/1017 ,  F17D3/00 ,  G01N21/75

Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.

Abstract translation: 公开了用于生物样品制备和分析的方法和装置。 装置可以具有容器的线性或圆形布置，其具有连接结构，例如杆或盘。 容器之间的流体通道允许执行不同的样品制备技术，如裂解，洗涤和洗脱。 可以将不同的功能元件，例如研磨机或混合器附接到容器。

公开(公告)号：US11109784B2

公开(公告)日：2021-09-07

申请号：US16191357

申请日：2018-11-14

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Dvin Adalian ,  Samson Chen ,  Muhammad M. Jilani ,  Axel Scherer ,  Xiomara Linnette Madero ,  Richard Smith

IPC: A61B5/1486 ,  A61B5/145 ,  A61B5/1473

Abstract: Measurement of target analytes is carried out with an enzyme-based sensor. The enzyme hydrogel is protected by a porous layer of a metallic material. The size of the pores is small enough to prevent degradation of the enzyme layer caused by the immune system of an organism, but large enough to allow transfer of molecules that participate in the electrochemical reaction allowing the enzyme to detect the target analytes.

公开(公告)号：US09518291B2

公开(公告)日：2016-12-13

申请号：US14070466

申请日：2013-11-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. Malik ,  Erika F. Garcia ,  Xiomara Linnette Madero ,  Axel Scherer

IPC: C12Q1/68 ,  C12P19/34 ,  F17D3/00

CPC classification number: C12Q1/686 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2400/0478 ,  F17D3/00 ,  G01N1/286 ,  G01N21/03 ,  G01N21/552 ,  G01N21/648 ,  G01N35/04 ,  G01N2001/2866 ,  G01N2001/4061 ,  G01N2021/0325 ,  G01N2035/00326 ,  G01N2035/00465 ,  G01N2035/0446 ,  G01N2035/1048 ,  G01N2035/1058 ,  G01N2201/0221 ,  Y02A50/58

Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers. The device may have a reaction cartridge with a reaction chamber to perform techniques such as polymerase chain reaction.

Abstract translation: 公开了用于生物样品制备和分析的方法和装置。 装置可以具有线性或圆形的容器布置，其具有连接结构，例如杆或盘。 容器之间的流体通道允许执行不同的样品制备技术，如裂解，洗涤和洗脱。 可以将不同的功能元件，例如研磨机或混合器附接到容器。 该装置可以具有带有反应室的反应筒，以进行诸如聚合酶链式反应的技术。

公开(公告)号：US09090891B2

公开(公告)日：2015-07-28

申请号：US14070465

申请日：2013-11-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Xiomara Linnette Madero ,  Imran R Malik ,  Erika F Garcia ,  Axel Scherer

IPC: G01N21/75 ,  C12N15/10 ,  F17D3/00

CPC classification number: C12N15/1017 ,  F17D3/00 ,  G01N21/75 ,  Y10T137/8593

Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a circular arrangement of containers, with a connecting structure such as a disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. The device may be pen-shaped and have a sample drawing needle connected to the containers.

Abstract translation: 公开了用于生物样品制备和分析的方法和装置。 装置可以具有圆形布置的容器，具有诸如盘的连接结构。 容器之间的流体通道允许执行不同的样品制备技术，如裂解，洗涤和洗脱。 该装置可以是笔形的并且具有连接到容器的抽样针。