公开(公告)号：US09284520B2

公开(公告)日：2016-03-15

申请号：US14070454

申请日：2013-11-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. Malik ,  Erika F. Garcia ,  Xiomara Linnette Madero ,  Axel Scherer

IPC: G01N21/00 ,  C12M1/40 ,  B01L3/00 ,  B01L9/00 ,  B01L7/00

CPC classification number: C12M21/18 ,  B01L3/0275 ,  B01L3/502707 ,  B01L3/502715 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/023 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1827 ,  B01L2400/0478

Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.

Abstract translation: 公开了用于生物样品制备和分析的方法和装置。 装置可以具有容器的线性或圆形布置，其具有连接结构，例如杆或盘。 容器之间的流体通道允许执行不同的样品制备技术，如裂解，洗涤和洗脱。 可以将不同的功能元件，例如研磨机或混合器附接到容器。

公开(公告)号：US08873055B2

公开(公告)日：2014-10-28

申请号：US13947469

申请日：2013-07-22

Applicant: California Institute of Technology

Inventor： Imran R. Malik ,  Xiomara L. Madero ,  Erika F. Garcia ,  Axel Scherer

IPC: G01N21/25 ,  G01N21/64

CPC classification number: G01N21/64 ,  G01N21/0303 ,  G01N21/645

Abstract: Structures and methods are described for optical detection of physical, chemical and/or biological samples. An optical detection structure may include a LED source, multiple filters and single or multiple sample areas. A detector may be used to record a fluorescence signal. The sample area may allow the introduction of removable cartridges.

Abstract translation: 描述了物理，化学和/或生物样品的光学检测的结构和方法。 光学检测结构可以包括LED源，多个滤光器和单个或多个样品区域。 检测器可用于记录荧光信号。 样品区域可能允许引入可移除的墨盒。

公开(公告)号：US11959856B2

公开(公告)日：2024-04-16

申请号：US15701014

申请日：2017-09-11

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. Malik ,  Xiomara Linnette Madero ,  Erika F. Garcia ,  Sheel Mukesh Shah ,  Axel Scherer

IPC: G01N21/64 ,  C12Q1/6851

CPC classification number: G01N21/6486 ,  C12Q1/6851 ,  C12Q1/6851 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2565/629

Abstract: Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.

公开(公告)号：US20230151440A1

公开(公告)日：2023-05-18

申请号：US17810696

申请日：2022-07-05

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

CPC classification number: C12Q1/701 ,  C12Q1/703 ,  C12Q1/68 ,  C12Q1/6851 ,  Y10T436/143333

Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US11414716B2

公开(公告)日：2022-08-16

申请号：US16827590

申请日：2020-03-23

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US10597737B2

公开(公告)日：2020-03-24

申请号：US16109288

申请日：2018-08-22

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: Methods and kits for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. At least one of the primers is configured to hybridize to a region of the polynucleotide analyte encoding the genetic variation. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US09518291B2

公开(公告)日：2016-12-13

申请号：US14070466

申请日：2013-11-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. Malik ,  Erika F. Garcia ,  Xiomara Linnette Madero ,  Axel Scherer

IPC: C12Q1/68 ,  C12P19/34 ,  F17D3/00

CPC classification number: C12Q1/686 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2400/0478 ,  F17D3/00 ,  G01N1/286 ,  G01N21/03 ,  G01N21/552 ,  G01N21/648 ,  G01N35/04 ,  G01N2001/2866 ,  G01N2001/4061 ,  G01N2021/0325 ,  G01N2035/00326 ,  G01N2035/00465 ,  G01N2035/0446 ,  G01N2035/1048 ,  G01N2035/1058 ,  G01N2201/0221 ,  Y02A50/58

Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers. The device may have a reaction cartridge with a reaction chamber to perform techniques such as polymerase chain reaction.

Abstract translation: 公开了用于生物样品制备和分析的方法和装置。 装置可以具有线性或圆形的容器布置，其具有连接结构，例如杆或盘。 容器之间的流体通道允许执行不同的样品制备技术，如裂解，洗涤和洗脱。 可以将不同的功能元件，例如研磨机或混合器附接到容器。 该装置可以具有带有反应室的反应筒，以进行诸如聚合酶链式反应的技术。

公开(公告)号：US09416343B2

公开(公告)日：2016-08-16

申请号：US14070440

申请日：2013-11-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. Malik ,  Axel Scherer ,  Erika F. Garcia ,  Xiomara L. Madero

IPC: G01N21/75 ,  G01N21/00 ,  G01N21/01 ,  C12M1/40 ,  B01L9/00 ,  G01N21/17 ,  B01L3/00 ,  B01L3/02 ,  B01L7/00

CPC classification number: C12M21/18 ,  B01L3/0275 ,  B01L3/502707 ,  B01L3/502715 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/023 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1827 ,  B01L2400/0478

Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.

Abstract translation: 公开了用于生物样品制备和分析的方法和装置。 装置可以具有容器的线性或圆形布置，其具有连接结构，例如杆或盘。 容器之间的流体通道允许执行不同的样品制备技术，如裂解，洗涤和洗脱。 可以将不同的功能元件，例如研磨机或混合器附接到容器。

公开(公告)号：US11879162B2

公开(公告)日：2024-01-23

申请号：US17810696

申请日：2022-07-05

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q1/68 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2565/101

Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US20210002732A1

公开(公告)日：2021-01-07

申请号：US16827590

申请日：2020-03-23

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.