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    METHOD FOR DISSOCIATING CELL AGGREGATES
    1.
    发明申请
    METHOD FOR DISSOCIATING CELL AGGREGATES 审中-公开

    公开(公告)号：US20190031990A1

    公开(公告)日：2019-01-31

    申请号：US16072379

    申请日：2017-01-25

    申请人： CENTRE FOR COMMERCIALIZATION OF REGENERATIVE MEDICINE

    发明人： Nicholas Timmins ,  Lesley Chan

    IPC分类号： C12M3/08 ,  C12M1/33 ,  C12M3/02 ,  C12M1/00 ,  C12N5/0735

    摘要： A method for dissociating cell aggregates in an agitated reactor. The method comprises providing a cell culture comprising cell aggregates in the agitated reactor, contacting the cell aggregates with a dissociation reagent, generating a dissociation force in the agitated reactor and exposing the contacted cell aggregates to the generated dissociation force under conditions sufficient to dissociate the contacted cell aggregates. The method may be used in a process for passaging cells and/or generating dissociated differentiated cells from stem and/or progenitor cells.

    Compositions and methods for sample preparation
    2.
    发明授权
    Compositions and methods for sample preparation 有权
    样品制备的组成和方法

    公开(公告)号：US09441265B2

    公开(公告)日：2016-09-13

    申请号：US14369627

    申请日：2012-12-28

    申请人： IBIS BIOSCIENCES, INC.

    发明人： Thomas N. Chiesl

    IPC分类号： C12Q1/68 ,  C12N1/06 ,  C12M1/00 ,  C12M1/33 ,  C12M3/08 ,  G01N33/552 ,  C12N15/10

    CPC分类号： C12Q1/6806 ,  C12N15/1006

    摘要： The invention relates generally to compositions and methods for disrupting cells (e.g., disrupting cellular and nuclear membranes). In particular, the invention provides modified solid surfaces (e.g., bead surfaces) and their use in disruption of cellular membranes (e.g., during cellular lysis procedures (e.g., for recovery of nucleic acid (e.g., DNA or RNA) from mechanical cell lysis)). Compositions and methods of the invention find use in a wide range of applications including molecular biology and medical science.

    摘要翻译： 本发明一般涉及破坏细胞（例如破坏细胞和核膜）的组合物和方法。 特别地，本发明提供修饰的固体表面（例如，珠粒表面）及其在破坏细胞膜中的用途（例如，在细胞裂解程序期间（例如，用于从机械细胞裂解中回收核酸（例如DNA或RNA）） ）。 本发明的组合物和方法可用于广泛的应用，包括分子生物学和医学科学。

    Biological specimen collection and transport system and method of use
    4.
    发明授权
    Biological specimen collection and transport system and method of use 有权
    生物样本采集和运输系统及使用方法

    公开(公告)号：US08415330B2

    公开(公告)日：2013-04-09

    申请号：US13632272

    申请日：2012-10-01

    申请人： Longhorn Vaccines & Diagnostics, LLC

    发明人： Gerald W. Fischer ,  Luke T. Daum

    IPC分类号： A61K31/66 ,  A61K31/19 ,  C12Q1/68 ,  C12M3/08 ,  C09K15/16

    CPC分类号： C12Q1/70 ,  C12Q1/6806 ,  C12Q2527/125

    摘要： Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.

    摘要翻译： 公开了用于从生物，法医和环境样品中分离核酸群体的组合物。 还公开了将这些组合物用作用于杀死病原体，灭活核酸酶以及从样品中的其他细胞组分释放多核苷酸的一步制剂以及在进一步加工或测定之前使核酸稳定的方法。 所公开的组合物安全地促进快速样品收集，并且在环境温度或升高的温度下提供样品的延长的储存和运输，而不会污染样品或其中所含的核酸的降解。 该方法特别有助于从远程位置收集标本，并且在先前被认为是敌对的条件下收集样品，以保持从裂解的生物样品中释放的核酸的完整性，而不需要在分子分析之前进行冷冻或冷冻。

    Tissue electro-sectioning apparatus
    6.
    发明申请
    Tissue electro-sectioning apparatus 审中-公开
    组织电切机

    公开(公告)号：US20050220674A1

    公开(公告)日：2005-10-06

    申请号：US11091873

    申请日：2005-03-28

    申请人： Gal Shafirstein ,  Scott Ferguson ,  Louis Fink ,  Richard Ulrich

    发明人： Gal Shafirstein ,  Scott Ferguson ,  Louis Fink ,  Richard Ulrich

    IPC分类号： B01L3/00 ,  B23Q15/24 ,  C12M3/08 ,  G01N1/04 ,  G01N1/06

    CPC分类号： G01N1/06 ,  C12M45/07 ,  G01N2001/045

    摘要： An apparatus for sectioning fresh unfixed tissue into very thin layers with preserved tissue architecture, antigenicity, mRNA content, and amenable to 3-D computer reconstruction without mechanical or thermal damage by employing a sectioning tool having an electrode with an intense focused electrical field at an edge. A computer controlled x-y-z translation stage moves the sectioning tool through the tissue as defined by a predetermined program. The sectioning tool produces consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analyses without mechanical or thermal damage, ultimately allowing high-resolution volumetric reconstruction of gene and protein expression patterns of large tissue specimens. The geometry of the sectioning tool is selected so as to produce a spatially localized electrical field of sufficient intensity to sever molecular bonds or propagate flaws in tissue without mechanical cutting.

    摘要翻译： 一种用于将新鲜未固定组织切割成具有保护的组织结构，抗原性，mRNA含量的非常薄的层的装置，并且适用于没有机械或热损伤的3-D计算机重建，通过采用具有电场强度聚焦电场的切片工具 边缘。 计算机控制的x-y-z平移台通过由预定程序定义的组织移动切片工具。 切片工具可生成连续薄片的新鲜组织，用于免疫组织化学和核酸分析，无机械或热损伤，最终允许高分辨率体积重建大组织标本的基因和蛋白质表达模式。 选择切片工具的几何形状以产生足够强度的空间局部电场，以切断分子键或传播组织中的缺陷而无需机械切割。

    Encapsulated cell device
    7.
    发明授权
    Encapsulated cell device 失效
    封装单元设备

    公开(公告)号：US5976780A

    公开(公告)日：1999-11-02

    申请号：US89174

    申请日：1998-06-02

    申请人： Kumarpal A. Shah

    发明人： Kumarpal A. Shah

    IPC分类号： A61K9/00 ,  C12M3/08 ,  C12N11/04 ,  A01N1/02

    CPC分类号： A61K9/0024 ,  C12N11/04

    摘要： A macroencapsulation device for somatic cells using ultrapurified Na alginate and polysulfone hollow fibers of 30 kDa molecular weight cutoff. Ultrapurified Na alginate material is used which has a high `G` content, low endotoxin content, low divalent metal toxins and low protein impurities. Islet cells prior to being encapsulated, are irrigated with Hank's modified solution (without Ca and Mg) containing gentamycin, vancomycin and amphotericin B and then passed through a leukoabsorb filter to reduce the donor antigen load of passenger leukocytes and to reduce the bioburden of microorganisms including viruses. Encapsulation is done in RPMI 1640 tissue culture fluid containing necessary nutritional supplements and ATP source of energy. The open ends of the fiber are covered with a porous membrane. To further improve biocompatibility, the outer wall of the polysulfone is lightly gelled with alginate gel. Such double gelled, encapsulated fibers do not affect diffusion of glucose or insulin across the hollow fibers.

    摘要翻译： 使用超疏水藻酸钠和30kDa分子量截止的聚砜中空纤维的体细胞的大包封装置。 使用具有高“G”含量，低内毒素含量，低二价金属毒素和低蛋白质杂质的超纯海藻酸盐材料。 在封装之前的胰岛细胞用含有庆大霉素，万古霉素和两性霉素B的汉克氏修饰溶液（不含Ca和Mg）灌注，然后通过白细胞吸收滤器以降低乘客白细胞的供体抗原载量并减少微生物的生物负载，包括 病毒 RPMI 1640组织培养液中含有必需的营养补充剂和ATP能量来进行封装。 纤维的开口端被多孔膜覆盖。 为了进一步提高生物相容性，聚砜的外壁用藻酸盐凝胶轻度凝胶化。 这种双胶凝的包封的纤维不影响葡萄糖或胰岛素穿过中空纤维的扩散。