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公开(公告)号：US20230392140A1
公开(公告)日：2023-12-07
申请号：US18304993
申请日：2023-04-21
CPC分类号： C12N15/1048 , C12N9/1276 , C12Y207/07049 , C12N15/1096
摘要： Disclosed herein are methods of reverse transcribing a polynucleotide comprising an unnatural ribonucleotide comprising reverse transcribing the polynucleotide with a reverse transcriptase in the presence of an unnatural dNTP comprising an unnatural nucleobase, wherein the reverse transcriptase polymerizes cDNA into which the unnatural NTP is incorporated. In some embodiments, the polynucleotide is present at a concentration less than or equal to about 500 nM and/or the polynucleotide is a tRNA, mRNA, RNA aptamer, or a member of a plurality of RNA aptamer candidates.
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公开(公告)号：US20240043823A1
公开(公告)日：2024-02-08
申请号：US18481086
申请日：2023-10-04
发明人： Jerod PTACIN , Carolina CAFFARO , Hans AERNI , Yorke ZHANG , Emil C. FISCHER , Aaron W. FELDMAN , Vivian T. DIEN , Floyd E. ROMESBERG
IPC分类号： C12N9/00 , C12P21/02 , C12N15/113 , C12N15/90
CPC分类号： C12N9/93 , C12P21/02 , C12Y601/01026 , C12N15/113 , C12N15/90
摘要： Disclosed herein are methods, compositions and kits for the synthesis of proteins which comprises unnatural amino acids that utilize a mutant tRNA.
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公开(公告)号：US20210222147A1
公开(公告)日：2021-07-22
申请号：US17196151
申请日：2021-03-09
发明人： Jerod PTACIN , Carolina CAFFARO , Hans AERNI , Yorke ZHANG , Emil C. FISCHER , Aaron W. FELDMAN , Vivian T. DIEN , Floyd E. ROMESBERG
IPC分类号： C12N9/00 , C12P21/02 , C12N15/113 , C12N15/90
摘要： Disclosed herein are methods, compositions and kits for the synthesis of proteins which comprises unnatural amino acids that utilize a mutant tRNA.
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公开(公告)号：US20200224234A1
公开(公告)日：2020-07-16
申请号：US16629211
申请日：2018-07-10
摘要： Disclosed herein are in vivo methods, compositions, and kits for producing nucleic acids which comprises at least one unnatural base. Disclosed herein are in vivo methods of producing a nucleic acid with an expanded genetic alphabet, the method comprising incorporatin at least one unnatural base in the nucleic acid. Disclosed herein are semi-synthetic organisms comprising an expanded genetic alphabet, wherein the genetic alphabet comprises at least one unnatural base. Disclosed herein are compositions that include a heterologous or recombinant polymerase and methods of use thereof. Further, disclosed herein are kits that are useful for stably incorporating an unnatural nucleic acid into a nucleic acid molecule, e.g., using the methods provided by the present invention in in vitro condition or under a cell free condition.
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5.发明申请公开(公告)号：US20200017540A1
公开(公告)日：2020-01-16
申请号：US16535992
申请日：2019-08-08
摘要： Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
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公开(公告)号：US20200318122A1
公开(公告)日：2020-10-08
申请号：US16913226
申请日：2020-06-26
IPC分类号： C12N15/67 , C12N9/22 , C12N15/113 , C12P19/34
摘要： Disclosed herein are methods, cells, engineered microorganisms, and kits for increasing the production of polypeptides comprising one or more unnatural amino acids. Further provided are cells, engineered microorganisms, and kits for increasing the retention of unnatural nucleic acids encoding the unnatural amino acids in an engineered cell, or semi-synthetic organism.
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公开(公告)号：US20190375802A1
公开(公告)日：2019-12-12
申请号：US16546097
申请日：2019-08-20
发明人： Floyd E. ROMESBERG , Yorke ZHANG
IPC分类号： C07K14/405 , C12N15/11 , C12N9/22 , C12N15/90
摘要： Disclosed herein are proteins, methods, cells, engineered microorganisms, and kits for generating a modified nucleoside triphosphate transporter from Phaeodactylum tricornutum. Also disclosed herein proteins, methods, cells, engineered microorganisms, and kits for production of a nucleic acid molecule that comprises an unnatural nucleotide utilizing a modified nucleoside triphosphate transporter from Phaeodactylum tricornutum.
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公开(公告)号：US20240117363A1
公开(公告)日：2024-04-11
申请号：US18228251
申请日：2023-07-31
发明人： Floyd E. ROMESBERG , Brian LAMB , Yorke ZHANG
CPC分类号： C12N15/63 , C12N5/10 , C12N9/22 , C12N15/11 , C12N15/70 , C12N2310/20 , C12N2310/3519
摘要： Disclosed herein are methods, cells, engineered microorganisms, and kits for increased production of a nucleic acid molecule that comprises an unnatural nucleotide.
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9.发明公开公开(公告)号：US20230235339A1
公开(公告)日：2023-07-27
申请号：US17881471
申请日：2022-08-04
IPC分类号： C12N15/70 , C12P19/34 , C12N15/82 , C07K14/295 , C07K14/405
CPC分类号： C12N15/70 , C12P19/34 , C12N15/8243 , C07K14/295 , C07K14/405
摘要： A recombinantly expressed nucleotide triphosphate transporter efficiently imports the triphosphates of unnatural nucleotides into cells, and the endogenous cellular machinery incorporates those nucleotides into cellular nucleic acids. UBPs can therefore form within the cell's nucleic acids. Moreover, neither the presence of the unnatural triphosphates nor the replication of the UBP represents a significant growth burden. The UBP is not efficiently excised by nucleic acid repair pathways, and therefore can be retained as long as the unnatural triphosphates are available in the growth medium. Thus, the resulting cell is the first organism to stably propagate an expanded genetic alphabet.
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公开(公告)号：US20220228148A1
公开(公告)日：2022-07-21
申请号：US17709041
申请日：2022-03-30
IPC分类号： C12N15/113 , C12P21/02
摘要： Provided herein are eukaryotic semi-synthetic organisms and their methods of use and manufacture.
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