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1.发明申请A METHOD FOR THE SITE-SPECIFIC ENZYMATIC LABELLING OF NUCLEIC ACIDS IN VITRO BY INCORPORATION OF UNNATURAL NUCLEOTIDES 审中-公开通过联合核心技术对体外核酸的特异性酶标技术的方法公开(公告)号：US20160168187A1
公开(公告)日：2016-06-16
申请号：US14910203
申请日：2014-08-08
CPC分类号： C07H19/24 , C07H19/00 , C07H21/00 , C12P19/34 , C12Q1/6832 , C12Q2525/117
摘要： Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
摘要翻译： 本文提供了在DNA聚合酶介导的DNA或RNA聚合酶介导的RNA转录的DNA聚合酶介导的复制期间，主要具有疏水性核碱基类似物的类似物的类似物，其形成非天然碱基对。 以这种方式，可以以位点特异性方式将非天然核碱基引入寡核苷酸（单链或双链DNA或RNA），其中它们可以提供位点特异性切割，或者可以提供比经过官能化的反应性连接体 通过与伯氨基反应或通过使用非天然核碱基连接体的炔基进行点击化学反应的载货试剂。
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2.发明申请公开(公告)号：US20200040027A1
公开(公告)日：2020-02-06
申请号：US16518715
申请日：2019-07-22
IPC分类号： C07H19/24 , C07H19/00 , C07H21/00 , C12Q1/6832 , C12P19/34
摘要： Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
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3.发明公开公开(公告)号：US20240101590A1
公开(公告)日：2024-03-28
申请号：US18112952
申请日：2023-02-22
IPC分类号： C07H19/24 , C07H19/00 , C07H21/00 , C07H21/04 , C12N9/22 , C12N15/10 , C12P19/34 , C12Q1/6832
CPC分类号： C07H19/24 , C07H19/00 , C07H21/00 , C07H21/04 , C12N9/22 , C12N15/1024 , C12P19/34 , C12Q1/6832
摘要： Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
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4.发明申请公开(公告)号：US20200017540A1
公开(公告)日：2020-01-16
申请号：US16535992
申请日：2019-08-08
摘要： Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
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