公开(公告)号：US20220243244A1

公开(公告)日：2022-08-04

申请号：US17716848

申请日：2022-04-08

申请人： The Scripps Research Institute

发明人： Floyd E. ROMESBERG ,  Emil C. FISCHER ,  Koji HASHIMOTO ,  Aaron W. FELDMAN ,  Vivian T. DIEN ,  Yorke ZHANG

IPC分类号： C12P21/02

摘要： Disclosed herein are compositions, methods, and kits for a cell incorporating unnatural amino acids into an unnatural polypeptide. Also disclosed herein are compositions, methods, and kits for increasing activity and yield of the unnatural polypeptide synthesized by the cell.

公开(公告)号：US20240294962A1

公开(公告)日：2024-09-05

申请号：US18532609

申请日：2023-12-07

申请人： The Scripps Research Institute

发明人： Floyd E. ROMESBERG ,  Vivian T. DIEN ,  Aaron W. FELDMAN ,  Rebekah J. KARADEEMA ,  Lingjun LI ,  Michael P. LEDBETTER ,  Anne Xiaozhou ZHOU

IPC分类号： C12P19/34 ,  C07H19/24 ,  C07H21/04

CPC分类号： C12P19/34 ,  C07H19/24 ,  C07H21/04

摘要： Disclosed herein are compositions, methods, cells, engineered microorganisms, and kits for increasing the production of proteins or polypeptides comprising one or more unnatural amino acids. Further provided are compositions, cells, engineered microorganisms, and kits for increasing the retention of unnatural nucleic acids encoding the unnatural amino acids in an engineered cell, or semi-synthetic organism.

公开(公告)号：US20210222147A1

公开(公告)日：2021-07-22

申请号：US17196151

申请日：2021-03-09

申请人： SYNTHORX, INC. ,  THE SCRIPPS RESEARCH INSTITUTE

发明人： Jerod PTACIN ,  Carolina CAFFARO ,  Hans AERNI ,  Yorke ZHANG ,  Emil C. FISCHER ,  Aaron W. FELDMAN ,  Vivian T. DIEN ,  Floyd E. ROMESBERG

IPC分类号： C12N9/00 ,  C12P21/02 ,  C12N15/113 ,  C12N15/90

摘要： Disclosed herein are methods, compositions and kits for the synthesis of proteins which comprises unnatural amino acids that utilize a mutant tRNA.

公开(公告)号：US20240043823A1

公开(公告)日：2024-02-08

申请号：US18481086

申请日：2023-10-04

申请人： Synthorx, Inc. ,  The Scripps Research Institute

发明人： Jerod PTACIN ,  Carolina CAFFARO ,  Hans AERNI ,  Yorke ZHANG ,  Emil C. FISCHER ,  Aaron W. FELDMAN ,  Vivian T. DIEN ,  Floyd E. ROMESBERG

IPC分类号： C12N9/00 ,  C12P21/02 ,  C12N15/113 ,  C12N15/90

CPC分类号： C12N9/93 ,  C12P21/02 ,  C12Y601/01026 ,  C12N15/113 ,  C12N15/90

摘要： Disclosed herein are methods, compositions and kits for the synthesis of proteins which comprises unnatural amino acids that utilize a mutant tRNA.

公开(公告)号：US20200224234A1

公开(公告)日：2020-07-16

申请号：US16629211

申请日：2018-07-10

申请人： THE SCRIPPS RESEARCH INSTITUTE

发明人： Floyd E. ROMESBERG ,  Yorke ZHANG ,  Aaron W. FELDMAN ,  Vivian T. DIEN

IPC分类号： C12P19/34 ,  C12N1/20 ,  C07H21/00

摘要： Disclosed herein are in vivo methods, compositions, and kits for producing nucleic acids which comprises at least one unnatural base. Disclosed herein are in vivo methods of producing a nucleic acid with an expanded genetic alphabet, the method comprising incorporatin at least one unnatural base in the nucleic acid. Disclosed herein are semi-synthetic organisms comprising an expanded genetic alphabet, wherein the genetic alphabet comprises at least one unnatural base. Disclosed herein are compositions that include a heterologous or recombinant polymerase and methods of use thereof. Further, disclosed herein are kits that are useful for stably incorporating an unnatural nucleic acid into a nucleic acid molecule, e.g., using the methods provided by the present invention in in vitro condition or under a cell free condition.