公开(公告)号：US20240316209A1

公开(公告)日：2024-09-26

申请号：US18044943

申请日：2021-09-10

申请人： BrickBio, Inc.

发明人： James Sebastian Italia ,  Nikolaos Biris

IPC分类号： A61K47/68 ,  C07K16/32 ,  C12N9/00

CPC分类号： A61K47/68031 ,  A61K47/6889 ,  C07K16/32 ,  C12N9/93 ,  C12Y601/01001 ,  C12Y601/01026

摘要： The invention relates generally to engineered antibodies containing unnatural amino acids (UAAs) and methods of making and using such antibodies.

公开(公告)号：US20240209409A1

公开(公告)日：2024-06-27

申请号：US18401960

申请日：2024-01-02

申请人： AJINOMOTO CO., INC.

发明人： Kenichi Haruna ,  Haruna Sato ,  Yoshihiko Matsuda ,  Kenichiro Ito

IPC分类号： C12P21/00 ,  C12N1/20 ,  C12N9/00 ,  C12R1/15

CPC分类号： C12P21/00 ,  C12N1/205 ,  C12N9/93 ,  C07K2319/10 ,  C12R2001/15 ,  C12Y601/01001 ,  C12Y601/01026

摘要： A method for secretory production of a protein containing a noncanonical amino acid is provided. Secretory production of a noncanonical amino acid-containing protein is carried out by culturing a coryneform bacterium having a genetic construct for secretory expression of a protein containing a noncanonical amino acid, which is modified to express an orthogonal pair of tRNA corresponding to the noncanonical amino acid and aminoacyl tRNA synthetase.

公开(公告)号：US20240043823A1

公开(公告)日：2024-02-08

申请号：US18481086

申请日：2023-10-04

申请人： Synthorx, Inc. ,  The Scripps Research Institute

发明人： Jerod PTACIN ,  Carolina CAFFARO ,  Hans AERNI ,  Yorke ZHANG ,  Emil C. FISCHER ,  Aaron W. FELDMAN ,  Vivian T. DIEN ,  Floyd E. ROMESBERG

IPC分类号： C12N9/00 ,  C12P21/02 ,  C12N15/113 ,  C12N15/90

CPC分类号： C12N9/93 ,  C12P21/02 ,  C12Y601/01026 ,  C12N15/113 ,  C12N15/90

摘要： Disclosed herein are methods, compositions and kits for the synthesis of proteins which comprises unnatural amino acids that utilize a mutant tRNA.

公开(公告)号：US20240252652A1

公开(公告)日：2024-08-01

申请号：US18565032

申请日：2022-06-02

申请人： The Regents of the University of California

发明人： Lei Wang ,  Shanshan Li

IPC分类号： A61K47/54 ,  A61K38/00 ,  C07D207/416 ,  C07K14/705 ,  C12N9/00 ,  C12Q1/6886

CPC分类号： A61K47/545 ,  C07D207/416 ,  C07K14/70596 ,  C12N9/93 ,  C12Q1/6886 ,  C12Y601/01026 ,  A61K38/00 ,  C12Q2600/154

摘要： Provided herein are, inter alia, compounds of Formula (I): biomolecules (e.g., proteins, lipids, RNA, glycans) comprising the compounds; bioconjugates comprising the compounds; processes for preparing the compounds, biomolecules, and bioconjugates; and their uses.

公开(公告)号：US11732001B2

公开(公告)日：2023-08-22

申请号：US16872826

申请日：2020-05-12

申请人： United Kingdom Research and Innovation

发明人： Jason Chin ,  Kathrin Lang

IPC分类号： C07K1/00 ,  G01N33/58 ,  C07K2/00 ,  C07C271/22 ,  C07K1/13 ,  C12N9/00

CPC分类号： C07K1/006 ,  C07C271/22 ,  C07K1/13 ,  C07K2/00 ,  C12N9/93 ,  C12Y601/01026 ,  G01N33/582 ,  C07C2602/24

摘要： The invention relates to a polypeptide comprising an amino acid having a bicyclo[6.1.0]non-4-yn-9-ylmethanol (BCN) group, particularly when said BCN group is present as: a residue of a lysine amino acid. The invention also relates to a method of producing a polypeptide comprising a BCN group, said method comprising genetically incorporating an amino acid comprising a BCN group into a polypeptide. The invention also relates to an amino acid comprising bicyclo[6.1.0]non-4-yn-9-ylmethanol (BCN), particularly and amino acid which is bicyclo[6.1.0]non-4-yn-9-ylmethanol (BCN) lysine. In addition the invention relates to a PylRS tRNA synthetase comprising the mutations Y271M, L274G and C313A.

公开(公告)号：US20180066298A1

公开(公告)日：2018-03-08

申请号：US15695831

申请日：2017-09-05

申请人： Ikaria Inc.

发明人： Stephen DiMagno

IPC分类号： C12P21/02 ,  C12N9/00 ,  C07K14/54 ,  C07C327/42 ,  C07B59/00

CPC分类号： C12P21/02 ,  C07B59/001 ,  C07B59/008 ,  C07B2200/05 ,  C07C233/51 ,  C07C327/42 ,  C07C327/44 ,  C07K14/54 ,  C07K14/545 ,  C12N9/93 ,  C12Y601/01026

摘要： Provided herein are compositions and methods for generating polypeptides using non-natural amino acids (nnAAs) and genetic machinery, wherein the modified polypeptides, such as therapeutic polypeptides, bind to albumin, such as serum albumin. Methods of substituting a non-natural amino acid in a first polypeptide to obtain a modified polypeptide, the nnAA in some instances comprising an albumin targeting group, are disclosed, as are methods for making populations of such modified polypeptides. A therapeutic polypeptide, interleukin-1 receptor antagonist (IL-1RA) is exemplified using the disclosed methods.

公开(公告)号：US20150005481A1

公开(公告)日：2015-01-01

申请号：US14373292

申请日：2013-01-21

申请人： MEDICAL RESEARCH COUNCIL ,  NORTH CAROLINA STATE UNIVERSITY

发明人： Jason Chin ,  Alexander Deiters

IPC分类号： A61K49/00 ,  G01N33/58 ,  C12P21/02 ,  C07K1/13 ,  C07C271/34

CPC分类号： A61K49/0017 ,  C07C271/34 ,  C07C2601/16 ,  C07C2602/42 ,  C07K1/13 ,  C12N9/93 ,  C12P21/02 ,  C12Y601/01026 ,  G01N33/582

摘要： The invention relates to a polypeptide comprising an amino acid having a norbornene group. Suitably said norbornene group is present as an amino acid residue of a norbornene lysine. The invention also relates to a method of producing a polypeptide comprising a norbornene group, said method comprising genetically incorporating an amino acid comprising a norbornene group into a polypeptide. The polypeptide comprising the norbornene group can be specifically labelled by inverse electron demand Diels-Alder reaction with a tetrazine compound.

摘要翻译： 本发明涉及包含具有降冰片烯基团的氨基酸的多肽。 降冰片烯基团合适地表示为降冰片烯赖氨酸的氨基酸残基。 本发明还涉及生产包含降冰片烯基团的多肽的方法，所述方法包括将包含降冰片烯基团的氨基酸遗传并入多肽中。 包含降冰片烯基团的多肽可以通过与四嗪化合物的反电子需求狄尔斯 - 阿尔德反应来具体标记。

公开(公告)号：US11939617B2

公开(公告)日：2024-03-26

申请号：US17540316

申请日：2021-12-02

申请人： Ikaria Inc.

发明人： Stephen DiMagno

IPC分类号： C12P21/02 ,  C07B59/00 ,  C07C233/51 ,  C07C327/42 ,  C07C327/44 ,  C07K14/54 ,  C07K14/545 ,  C12N9/00

CPC分类号： C12P21/02 ,  C07B59/001 ,  C07B59/008 ,  C07C233/51 ,  C07C327/42 ,  C07C327/44 ,  C07K14/54 ,  C07K14/545 ,  C12N9/93 ,  C12Y601/01026 ,  C07B2200/05

摘要： Provided herein are compositions and methods for generating polypeptides using non-natural amino acids (nnAAs) and genetic machinery, wherein the modified polypeptides, such as therapeutic polypeptides, bind to albumin, such as serum albumin. Methods of substituting a non-natural amino acid in a first polypeptide to obtain a modified polypeptide, the nnAA in some instances comprising an albumin targeting group, are disclosed, as are methods for making populations of such modified polypeptides. A therapeutic polypeptide, interleukin-1 receptor antagonist (IL-1RA) is exemplified using the disclosed methods.

公开(公告)号：US11834689B2

公开(公告)日：2023-12-05

申请号：US17196151

申请日：2021-03-09

申请人： SYNTHORX, INC. ,  THE SCRIPPS RESEARCH INSTITUTE

发明人： Jerod Ptacin ,  Carolina Caffaro ,  Hans Aerni ,  Yorke Zhang ,  Emil C. Fischer ,  Aaron W. Feldman ,  Vivian T. Dien ,  Floyd E. Romesberg

IPC分类号： C12N9/00 ,  C12P21/02 ,  C12N15/113 ,  C12N15/90

CPC分类号： C12N9/93 ,  C12N15/113 ,  C12N15/90 ,  C12P21/02 ,  C12Y601/01026

摘要： Disclosed herein are methods, compositions and kits for the synthesis of proteins which comprises unnatural amino acids that utilize a mutant tRNA.

公开(公告)号：US20140148576A1

公开(公告)日：2014-05-29

申请号：US14129028

申请日：2012-06-22

申请人： Jason Chin ,  Satpal Virdee

发明人： Jason Chin ,  Satpal Virdee

IPC分类号： C12P21/00 ,  C07C271/22 ,  C07C323/59 ,  C12N9/10

CPC分类号： C12P21/00 ,  C07C271/22 ,  C07C323/59 ,  C07K2319/95 ,  C12N9/104 ,  C12N9/93 ,  C12Y601/01026

摘要： The invention related to a tRNA synthetase capable of binding delta-substituted lysine, wherein said tRNA synthetase comprises amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS, wherein said sequence comprises 5 or fewer substitutions within the amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS; and wherein said synthetase comprises W at amino acid position 349 relative to MbPyIRS.

摘要翻译： 本发明涉及能够结合δ-取代的赖氨酸的tRNA合成酶，其中所述tRNA合成酶包含对应于MbPyIRS的至少L271至Y349的氨基酸序列的氨基酸序列，其中所述序列在氨基酸内包含5个或更少个取代 对应于MbPyIRS的至少L271至Y349的氨基酸序列的序列; 并且其中所述合成酶在相对于MbPyIRS的氨基酸位置349处包含W。