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    Methods and compositions for labeling nucleic acids
    2.
    发明授权
    Methods and compositions for labeling nucleic acids 有权
    用于标记核酸的方法和组合物

    公开(公告)号：US08541570B2

    公开(公告)日：2013-09-24

    申请号：US12785999

    申请日：2010-05-24

    Applicant: Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    Inventor： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    IPC: C07H19/06 ,  C07F9/02 ,  G01N33/48

    CPC classification number: C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/5011 ,  G01N33/582 ,  C12Q2525/101 ,  C12Q2563/107

    Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

    Abstract translation: 本发明涉及体外和体内标记核酸聚合物的方法。 提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。 提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。 这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。 还提供了测量细胞增殖的方法。 在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。 本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

    METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS 有权
    标记核酸的方法和组合物

    公开(公告)号：US20100311063A1

    公开(公告)日：2010-12-09

    申请号：US12785999

    申请日：2010-05-24

    Applicant: Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    Inventor： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/5011 ,  G01N33/582 ,  C12Q2525/101 ,  C12Q2563/107

    Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

    Abstract translation: 本发明涉及体外和体内标记核酸聚合物的方法。 提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。 提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。 这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。 还提供了测量细胞增殖的方法。 在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。 本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

    Methods and compositions for labeling nucleic acids
    8.
    发明授权
    Methods and compositions for labeling nucleic acids 有权
    用于标记核酸的方法和组合物

    公开(公告)号：US07767421B2

    公开(公告)日：2010-08-03

    申请号：US11588697

    申请日：2006-10-27

    Applicant: Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    Inventor： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    IPC: C12P19/34 ,  C07H21/02

    CPC classification number: C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/5011 ,  G01N33/582 ,  C12Q2525/101 ,  C12Q2563/107

    Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

    Abstract translation: 本发明涉及体外和体内标记核酸聚合物的方法。 提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。 提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。 这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。 还提供了测量细胞增殖的方法。 在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。 本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

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