公开(公告)号：US20150362469A1

公开(公告)日：2015-12-17

申请号：US14815574

申请日：2015-07-31

申请人： Andreas Mershin ,  Asmamaw Wassie ,  Yael Maguire ,  David Kong ,  Shuguang Zhang ,  Patrick Moran ,  Karolina Corin

发明人： Andreas Mershin ,  Asmamaw Wassie ,  Yael Maguire ,  David Kong ,  Shuguang Zhang ,  Patrick Moran ,  Karolina Corin

IPC分类号： G01N33/00

CPC分类号： G01N33/0031 ,  G01N27/00

摘要： In exemplary implementations of this invention, an electronic olfactor determines whether a scent being tested matches the scent of a positive control. The electronic olfactor can perform this scent matching even in a changing olfactory environment, and even if the positive control scent is a combination of hundreds or thousands of different odorants. No prior training is needed, and no attempt is made to identify a single odorant that is unambiguously responsible for a scent. Instead, a computer compares the total scent pattern of a positive control sample with the total scent pattern of a test sample, across a sweep of many permutations of electrical inputs to scent sensors, to try to find any condition under which the total scent patterns do not match. If such a condition cannot be found, then the computer declares a match between the test and target scents.

摘要翻译： 在本发明的示例性实施方案中，电子嗅觉元件确定被测试的香味是否与阳性对照的香味相匹配。 电子嗅觉剂即使在不断变化的嗅觉环境中也可以进行这种气味的匹配，即使阳性对照气味是数百种或数千种不同气味剂的组合。 不需要进行任何先前的培训，也没有尝试确定一个明确负责香味的单一加臭剂。 相反，计算机将阳性对照样品的总气味模式与测试样品的总气味模式进行比较，以扫描多次电气输入到气味传感器的排列，以尝试找到总气味图案的任何条件 不匹配。 如果找不到这样的条件，那么计算机会声明测试和目标气味之间的匹配。

公开(公告)号：US20120108041A1

公开(公告)日：2012-05-03

申请号：US13347662

申请日：2012-01-10

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L21/20

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including a definition of a charge pattern on a substrate and introduction of charged molecular scale sized building blocks (MSSBBs) to a region proximate the charge pattern so that the MSSBBs adhere to the charge pattern to form the feature.

摘要翻译： 一种用于形成纳米结构的技术，其包括基板上的电荷图案的定义，以及将电荷分子尺度的结构单元（MSSBB）引入邻近电荷图案的区域，使得MSSBB粘附于电荷图案以形成特征。

公开(公告)号：US08093144B2

公开(公告)日：2012-01-10

申请号：US10444176

申请日：2003-05-23

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L21/20

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including a definition of a charge pattern on a substrate and introduction of charged molecular scale sized building blocks (MSSBBs) to a region proximate the charge pattern so that the MSSBBs adhere to the charge pattern to form the feature.

摘要翻译： 一种用于形成纳米结构的技术，其包括在衬底上的电荷图案的定义，以及将电荷分子尺度的结构单元（MSSBB）引入邻近电荷图案的区域，使得MSSBB粘附于电荷图案以形成特征。

公开(公告)号：US20090156427A1

公开(公告)日：2009-06-18

申请号：US12183916

申请日：2008-07-31

申请人： Shuguang Zhang ,  Andreas Mershin ,  Liselotte Kaiser ,  Brian Cook ,  Johanna F. Graveland-Bikker ,  Manu Prakash ,  David Kong ,  Yael Maguire

发明人： Shuguang Zhang ,  Andreas Mershin ,  Liselotte Kaiser ,  Brian Cook ,  Johanna F. Graveland-Bikker ,  Manu Prakash ,  David Kong ,  Yael Maguire

IPC分类号： C40B30/10 ,  B01J19/00 ,  C40B40/10 ,  C40B60/12 ,  C40B30/00 ,  C12P21/00

CPC分类号： G01N33/54366 ,  G01N2333/726

摘要： The invention provides a bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—on a support, a real time receptor-ligand binding detection method, an odorant delivery system and an odorant recognition program. The biologically-derived G-protein coupled receptor can be stabilized on nanotechnology using surfactant peptide. The said nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor.

摘要翻译： 本发明提供一种生物感测纳米装置，其包括：稳定的生物衍生的G蛋白偶联受体 - 支持物上的嗅觉受体，实时受体 - 配体结合检测方法，加味剂递送系统和气味识别程序。 生物衍生的G蛋白偶联受体可以使用表面活性剂肽在纳米技术上稳定。 所述纳米器件提供更大的表面积，以提高气味检测的精度和灵敏度。 本发明进一步提供了一种微流控芯片，其包含稳定的生物衍生的G蛋白偶联受体 - 嗅觉受体 - 固定在载体上，并且布置在至少二维微阵列系统中。 本发明还提供了一种递送加臭剂的方法，其包括在复杂微流体网络中操作气泡的步骤，其中气泡在携带各种气体样品的微流体通道中移动到芯片上的精确位置。 本发明还提供了制备hOR17-4嗅觉受体的方法。

公开(公告)号：US09140677B2

公开(公告)日：2015-09-22

申请号：US13966169

申请日：2013-08-13

申请人： Andreas Mershin ,  Asmamaw Wassie ,  Yael Maguire ,  David Kong ,  Shuguang Zhang ,  Patrick Moran ,  Karolina Corin

发明人： Andreas Mershin ,  Asmamaw Wassie ,  Yael Maguire ,  David Kong ,  Shuguang Zhang ,  Patrick Moran ,  Karolina Corin

IPC分类号： G01N33/00 ,  G01N27/00

CPC分类号： G01N33/0031 ,  G01N27/00

摘要： In exemplary implementations of this invention, an electronic olfactor determines whether a scent being tested matches the scent of a positive control. The electronic olfactor can perform this scent matching even in a changing olfactory environment, and even if the positive control scent is a combination of hundreds or thousands of different odorants. No prior training is needed, and no attempt is made to identify a single odorant that is unambiguously responsible for a scent. Instead, a computer compares the total scent pattern of a positive control sample with the total scent pattern of a test sample, across a sweep of many permutations of electrical inputs to scent sensors, to try to find any condition under which the total scent patterns do not match. If such a condition cannot be found, then the computer declares a match between the test and target scents.

摘要翻译： 在本发明的示例性实施方案中，电子嗅觉元件确定被测试的香味是否与阳性对照的香味相匹配。 电子嗅觉剂即使在不断变化的嗅觉环境中也可以进行这种气味的匹配，即使阳性对照气味是数百种或数千种不同气味剂的组合。 不需要进行任何先前的培训，也没有尝试确定一个明确负责香味的单一加臭剂。 相反，计算机将阳性对照样品的总香味图案与测试样品的总香味图案进行比较，以扫描许多排列的电气输入到香味传感器，以尝试找到任何条件，在这些条件下总香味图案 不匹配。 如果找不到这样的条件，那么计算机会声明测试和目标气味之间的匹配。

公开(公告)号：US20150064791A1

公开(公告)日：2015-03-05

申请号：US14529080

申请日：2014-10-30

申请人： David Kong ,  Peter A. Carr ,  Joseph M. Jacobson

发明人： David Kong ,  Peter A. Carr ,  Joseph M. Jacobson

IPC分类号： C12N15/90 ,  C12N15/70

CPC分类号： C12N15/902 ,  C12N15/70 ,  C12P19/34

摘要： A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol is applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog.

摘要翻译： 在微流体装置内直接合成来自寡核苷酸前体的长DNA构建体的方法一次使用若干寡核苷酸。 将含有至少两种具有至少部分碱基互补性的寡核苷酸前体的前体混合物引入微流体芯片的输入中，并应用至少一个基因合成方案的至少一个循环以制备含有至少两个序列的DNA构建体 寡核苷酸前体。 用于合成修饰的DNA构建体的方法包括将编码至少一个点突变的至少一种寡核苷酸电穿孔并与靶细胞的至少一个DNA区域具有同源性，并将寡核苷酸并入靶细胞DNA中，通过 重组蛋白β或重组蛋白β功能同源物的作用。

公开(公告)号：US08937001B2

公开(公告)日：2015-01-20

申请号：US13347662

申请日：2012-01-10

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L21/20 ,  B82Y10/00 ,  C23C14/02 ,  C23C14/04 ,  C23C14/22 ,  C23C16/04 ,  C23C16/455

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including a definition of a charge pattern on a substrate and introduction of charged molecular scale sized building blocks (MSSBBs) to a region proximate the charge pattern so that the MSSBBs adhere to the charge pattern to form the feature.

摘要翻译： 一种用于形成纳米结构的技术，其包括在衬底上的电荷图案的定义，以及将电荷分子尺度的结构单元（MSSBB）引入邻近电荷图案的区域，使得MSSBB粘附于电荷图案以形成特征。

公开(公告)号：US20140099729A1

公开(公告)日：2014-04-10

申请号：US13966169

申请日：2013-08-13

申请人： Andreas Mershin ,  Asmamaw Wassie ,  Yael Maguire ,  David Kong ,  Shuguang Zhang ,  Patrick Moran ,  Karolina Corin

发明人： Andreas Mershin ,  Asmamaw Wassie ,  Yael Maguire ,  David Kong ,  Shuguang Zhang ,  Patrick Moran ,  Karolina Corin

IPC分类号： G01N33/00

CPC分类号： G01N33/0031 ,  G01N27/00

摘要： In exemplary implementations of this invention, an electronic olfactor determines whether a scent being tested matches the scent of a positive control. The electronic olfactor can perform this scent matching even in a changing olfactory environment, and even if the positive control scent is a combination of hundreds or thousands of different odorants. No prior training is needed, and no attempt is made to identify a single odorant that is unambiguously responsible for a scent. Instead, a computer compares the total scent pattern of a positive control sample with the total scent pattern of a test sample, across a sweep of many permutations of electrical inputs to scent sensors, to try to find any condition under which the total scent patterns do not match. If such a condition cannot be found, then the computer declares a match between the test and target scents.

摘要翻译： 在本发明的示例性实施方案中，电子嗅觉元件确定被测试的香味是否与阳性对照的香味相匹配。 电子嗅觉剂即使在不断变化的嗅觉环境中也可以进行这种气味的匹配，即使阳性对照气味是数百种或数千种不同气味剂的组合。 不需要进行任何先前的培训，也没有尝试确定一个明确负责香味的单一加臭剂。 相反，计算机将阳性对照样品的总香味图案与测试样品的总香味图案进行比较，以扫描许多排列的电气输入到香味传感器，以尝试找到任何条件，在这些条件下总香味图案 不匹配。 如果找不到这样的条件，那么计算机会声明测试和目标气味之间的匹配。

公开(公告)号：US20160079060A1

公开(公告)日：2016-03-17

申请号：US14788723

申请日：2015-06-30

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L21/02 ,  H01L21/285 ,  H01L21/263

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including introducing a plurality of molecular-size scale and/or nanoscale building blocks to a region near a substrate and simultaneously scanning a pattern on the substrate with an energy beam, wherein the energy beam causes a change in at least one physical property of at least a portion of the building blocks, such that a probability of the portion of the building blocks adhering to the pattern scanned by the energy beam is increased, and wherein the building blocks adhere to the pattern to form the structure. The energy beam and at least a portion of the building blocks may interact by electrostatic interaction to form the structure.

公开(公告)号：US09070556B2

公开(公告)日：2015-06-30

申请号：US14600676

申请日：2015-01-20

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L51/00 ,  H01L21/02 ,  H01L21/285 ,  H01L27/30

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including introducing a plurality of molecular-size scale and/or nanoscale building blocks to a region near a substrate and simultaneously scanning a pattern on the substrate with an energy beam, wherein the energy beam causes a change in at least one physical property of at least a portion of the building blocks, such that a probability of the portion of the building blocks adhering to the pattern scanned by the energy beam is increased, and wherein the building blocks adhere to the pattern to form the structure. The energy beam and at least a portion of the building blocks may interact by electrostatic interaction to form the structure.

摘要翻译： 一种用于形成纳米结构的技术，包括将多个分子尺度尺度和/或纳米尺度结构单元引入到衬底附近的区域，同时用能量束扫描衬底上的图案，其中能量束引起至少一个 构造块的至少一部分的物理性质，使得构成块的部分附着到由能量束扫描的图案的概率增加，并且其中构建块粘附到图案以形成结构。 能量束和至少一部分构建块可通过静电相互作用而形成结构。