公开(公告)号：US10030253B2

公开(公告)日：2018-07-24

申请号：US14529080

申请日：2014-10-30

申请人： David Kong ,  Peter A. Carr ,  Joseph M. Jacobson

发明人： David Kong ,  Peter A. Carr ,  Joseph M. Jacobson

IPC分类号： C12Q1/68 ,  C12N15/90 ,  C12P19/34 ,  C12N15/70

摘要： A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol is applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog.

公开(公告)号：US20150064791A1

公开(公告)日：2015-03-05

申请号：US14529080

申请日：2014-10-30

申请人： David Kong ,  Peter A. Carr ,  Joseph M. Jacobson

发明人： David Kong ,  Peter A. Carr ,  Joseph M. Jacobson

IPC分类号： C12N15/90 ,  C12N15/70

CPC分类号： C12N15/902 ,  C12N15/70 ,  C12P19/34

摘要： A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol is applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog.

摘要翻译： 在微流体装置内直接合成来自寡核苷酸前体的长DNA构建体的方法一次使用若干寡核苷酸。 将含有至少两种具有至少部分碱基互补性的寡核苷酸前体的前体混合物引入微流体芯片的输入中，并应用至少一个基因合成方案的至少一个循环以制备含有至少两个序列的DNA构建体 寡核苷酸前体。 用于合成修饰的DNA构建体的方法包括将编码至少一个点突变的至少一种寡核苷酸电穿孔并与靶细胞的至少一个DNA区域具有同源性，并将寡核苷酸并入靶细胞DNA中，通过 重组蛋白β或重组蛋白β功能同源物的作用。

公开(公告)号：US20160079060A1

公开(公告)日：2016-03-17

申请号：US14788723

申请日：2015-06-30

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L21/02 ,  H01L21/285 ,  H01L21/263

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including introducing a plurality of molecular-size scale and/or nanoscale building blocks to a region near a substrate and simultaneously scanning a pattern on the substrate with an energy beam, wherein the energy beam causes a change in at least one physical property of at least a portion of the building blocks, such that a probability of the portion of the building blocks adhering to the pattern scanned by the energy beam is increased, and wherein the building blocks adhere to the pattern to form the structure. The energy beam and at least a portion of the building blocks may interact by electrostatic interaction to form the structure.

公开(公告)号：US09070556B2

公开(公告)日：2015-06-30

申请号：US14600676

申请日：2015-01-20

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L51/00 ,  H01L21/02 ,  H01L21/285 ,  H01L27/30

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including introducing a plurality of molecular-size scale and/or nanoscale building blocks to a region near a substrate and simultaneously scanning a pattern on the substrate with an energy beam, wherein the energy beam causes a change in at least one physical property of at least a portion of the building blocks, such that a probability of the portion of the building blocks adhering to the pattern scanned by the energy beam is increased, and wherein the building blocks adhere to the pattern to form the structure. The energy beam and at least a portion of the building blocks may interact by electrostatic interaction to form the structure.

摘要翻译： 一种用于形成纳米结构的技术，包括将多个分子尺度尺度和/或纳米尺度结构单元引入到衬底附近的区域，同时用能量束扫描衬底上的图案，其中能量束引起至少一个 构造块的至少一部分的物理性质，使得构成块的部分附着到由能量束扫描的图案的概率增加，并且其中构建块粘附到图案以形成结构。 能量束和至少一部分构建块可通过静电相互作用而形成结构。

公开(公告)号：US20150132928A1

公开(公告)日：2015-05-14

申请号：US14600676

申请日：2015-01-20

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L21/02 ,  H01L21/285

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including introducing a plurality of molecular-size scale and/or nanoscale building blocks to a region near a substrate and simultaneously scanning a pattern on the substrate with an energy beam, wherein the energy beam causes a change in at least one physical property of at least a portion of the building blocks, such that a probability of the portion of the building blocks adhering to the pattern scanned by the energy beam is increased, and wherein the building blocks adhere to the pattern to form the structure. The energy beam and at least a portion of the building blocks may interact by electrostatic interaction to form the structure.

摘要翻译： 一种用于形成纳米结构的技术，包括将多个分子尺度尺度和/或纳米尺度结构单元引入到衬底附近的区域，同时用能量束扫描衬底上的图案，其中能量束引起至少一个 构造块的至少一部分的物理性质，使得构成块的部分附着到由能量束扫描的图案的概率增加，并且其中构建块粘附到图案以形成结构。 能量束和至少一部分构建块可通过静电相互作用而形成结构。

公开(公告)号：US08937001B2

公开(公告)日：2015-01-20

申请号：US13347662

申请日：2012-01-10

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L21/20 ,  B82Y10/00 ,  C23C14/02 ,  C23C14/04 ,  C23C14/22 ,  C23C16/04 ,  C23C16/455

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including a definition of a charge pattern on a substrate and introduction of charged molecular scale sized building blocks (MSSBBs) to a region proximate the charge pattern so that the MSSBBs adhere to the charge pattern to form the feature.

摘要翻译： 一种用于形成纳米结构的技术，其包括在衬底上的电荷图案的定义，以及将电荷分子尺度的结构单元（MSSBB）引入邻近电荷图案的区域，使得MSSBB粘附于电荷图案以形成特征。

公开(公告)号：US20120108041A1

公开(公告)日：2012-05-03

申请号：US13347662

申请日：2012-01-10

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L21/20

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including a definition of a charge pattern on a substrate and introduction of charged molecular scale sized building blocks (MSSBBs) to a region proximate the charge pattern so that the MSSBBs adhere to the charge pattern to form the feature.

摘要翻译： 一种用于形成纳米结构的技术，其包括基板上的电荷图案的定义，以及将电荷分子尺度的结构单元（MSSBB）引入邻近电荷图案的区域，使得MSSBB粘附于电荷图案以形成特征。

公开(公告)号：US08093144B2

公开(公告)日：2012-01-10

申请号：US10444176

申请日：2003-05-23

申请人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

发明人： Joseph M. Jacobson ,  David Kong ,  Vikas Anant ,  Ashley Salomon ,  Saul Griffith ,  Will DelHagen ,  Vikrant Agnihotri

IPC分类号： H01L21/20

CPC分类号： H01L21/02631 ,  B82Y10/00 ,  B82Y40/00 ,  C23C14/024 ,  C23C14/048 ,  C23C14/228 ,  C23C16/047 ,  C23C16/45525 ,  C23C16/45555 ,  H01L21/02225 ,  H01L21/02266 ,  H01L21/0228 ,  H01L21/02351 ,  H01L21/0262 ,  H01L21/02664 ,  H01L21/2633 ,  H01L21/2855 ,  H01L21/28556 ,  H01L27/30 ,  H01L51/0002 ,  Y10S977/888 ,  Y10S977/891

摘要： A technique for forming nanostructures including a definition of a charge pattern on a substrate and introduction of charged molecular scale sized building blocks (MSSBBs) to a region proximate the charge pattern so that the MSSBBs adhere to the charge pattern to form the feature.

摘要翻译： 一种用于形成纳米结构的技术，其包括在衬底上的电荷图案的定义，以及将电荷分子尺度的结构单元（MSSBB）引入邻近电荷图案的区域，使得MSSBB粘附于电荷图案以形成特征。

公开(公告)号：US20070281309A1

公开(公告)日：2007-12-06

申请号：US11751604

申请日：2007-05-21

申请人： David Kong ,  Peter Carr ,  Joseph Jacobson

发明人： David Kong ,  Peter Carr ,  Joseph Jacobson

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12N15/902 ,  C12N15/70 ,  C12P19/34

摘要： A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol are applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog.

摘要翻译： 在微流体装置内直接合成来自寡核苷酸前体的长DNA构建体的方法一次使用若干寡核苷酸。 将包含至少两种具有至少部分碱基互补性的寡核苷酸前体的前体混合物引入微流体芯片的输入中，并且至少一个基因合成方案的至少一个循环被应用于制备含有至少两个序列的DNA构建体 寡核苷酸前体。 用于合成修饰的DNA构建体的方法包括将编码至少一个点突变的至少一种寡核苷酸电穿孔并与靶细胞的至少一个DNA区域具有同源性，并将寡核苷酸并入靶细胞DNA中，通过 重组蛋白β或重组蛋白β功能同源物的作用。

公开(公告)号：US20130149724A1

公开(公告)日：2013-06-13

申请号：US13682710

申请日：2012-11-20

申请人： Ashok C. Chander ,  David Kong

发明人： Ashok C. Chander ,  David Kong

IPC分类号： G01N33/50

CPC分类号： G01N33/5008 ,  C12M23/22 ,  C12M23/58 ,  C12M45/02 ,  C12M45/09 ,  C12M47/04 ,  G01N33/54386 ,  G01N33/574

摘要： Microfluidic devices for dissociating tissue, culturing, separating, manipulating, and assaying cells and methods for using the device are disclosed. Individual modules for tissue dissociation, cell, protein and particle separation, cell adhesion to functionalized, permissive micro- and nano-substrates, cell culturing, cell manipulation, cell and extracellular component assaying via metabolic and therapeutic compounds, compound titration, cell transfection, and micro-ELISA are described. Specialized micro- and nano-substrates and their methods of fabrication are also described. An integrated device is also disclosed. The devices and methods can be used for diagnostic applications, monitoring of disease progression, analysis of disease recurrence, compound discovery, compound validation, drug efficacy screening, and cell-based assays.

摘要翻译： 公开了用于解离组织，培养，分离，操作和测定细胞的微流体装置和用于使用该装置的方法。 用于组织解离，细胞，蛋白质和颗粒分离，细胞粘附到功能化，允许的微和纳米底物，细胞培养，细胞操作，通过代谢和治疗化合物的细胞和细胞外成分测定的单个模块，复合滴定，细胞转染和 描述了微型ELISA。 还描述了专门的微纳米基板及其制造方法。 还公开了一种集成器件。 设备和方法可用于诊断应用，疾病进展监测，疾病复发分析，化合物发现，化合物验证，药物疗效筛选和基于细胞的检测。