公开(公告)号：US20130183747A1

公开(公告)日：2013-07-18

申请号：US13787211

申请日：2013-03-06

申请人： Masakazu Fukuda ,  Hironori Kobayashi ,  Junyi Ding ,  Ryuichiro Ebi ,  Koki Tajima

发明人： Masakazu Fukuda ,  Hironori Kobayashi ,  Junyi Ding ,  Ryuichiro Ebi ,  Koki Tajima

IPC分类号： G01N1/34

CPC分类号： G01N1/34 ,  G01N15/0618 ,  G01N15/147 ,  G01N15/1475 ,  G01N35/1095 ,  G01N2015/1006 ,  G01N2015/1493 ,  G01N2035/00475

摘要： A cell processing apparatus includes a storage container that contains liquid L including a biological sample; a filter that prevents a first cell C1 in the biological sample from passing and allows a second cell C2 having a smaller diameter than that of the first cell C1 to pass; and a filtration cylinder for separating, in the storage container and via the filter, the liquid L into a first liquid L1 mainly including the first cell C1 and a second liquid L2 mainly including the second cell C2. A measurement target cell filtered by the filter among other cells can be easily collected.

摘要翻译： 电池处理装置包括：容纳包含生物样品的液体L的储存容器; 防止生物样品中的第一细胞C1通过并允许具有比第一细胞C1的直径小的第二细胞C2通过的过滤器; 以及过滤筒，其在储存容器中并通过过滤器将液体L分离成主要包括第一单元C1的第一液体L1和主要包括第二单元C2的第二液体L2。 可以容易地收集由其他单元之间的过滤器过滤的测量目标单元。

公开(公告)号：US20130162795A1

公开(公告)日：2013-06-27

申请号：US13757044

申请日：2013-02-01

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Connie C. WONG ,  Kevin E. Loewke ,  Thomas M. Baer ,  Renee A. Reijo-Pera ,  Barry Behr

IPC分类号： G02B21/00

CPC分类号： G01N15/10 ,  A61B17/435 ,  C12M21/06 ,  C12M41/36 ,  C12M41/46 ,  C12M41/48 ,  C12N5/0604 ,  C12Q1/02 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N15/1475 ,  G01N33/5005 ,  G01N2015/1087 ,  G01N2015/1093 ,  G01N2015/1493 ,  G01N2015/1497 ,  G02B21/0004 ,  G06F19/26 ,  G06K9/00147 ,  G06T7/0012 ,  G06T2207/10004 ,  G06T2207/10056 ,  G06T2207/30044

摘要： Methods, compositions and kits for determining the developmental potential of one or more embryos or pluripotent cells and/or the presence of chromosomal abnormalities in one or more embryos or pluripotent cells are provided. These methods, compositions and kits find use in identifying embryos and oocytes in vitro that are most useful in treating infertility in humans.

公开(公告)号：US08441624B2

公开(公告)日：2013-05-14

申请号：US13528016

申请日：2012-06-20

申请人： Thomas J. Matula ,  Jarred Swalwell

发明人： Thomas J. Matula ,  Jarred Swalwell

IPC分类号： G01N21/00

CPC分类号： G01N21/49 ,  A61B5/0059 ,  A61B8/481 ,  G01N15/1459 ,  G01N21/01 ,  G01N21/70 ,  G01N33/15 ,  G01N2015/1493 ,  G01N2021/0193

摘要： Flow cytometry concepts are modified to enable dynamic characterizations of particles to be obtained using optical scattering data. Particles in flow will be introduced into a sample volume. Light scattered by a particle in the sample volume is collected and analyzed. What differentiates the concepts disclosed herein from conventional flow cytometry is the use of an acoustic source that is disposed to direct acoustic energy into the sample volume. As the particle passes through the sample volume, it responds to the acoustic energy, causing changes in the light scattered by the particle. Those changes, which are not measured during conventional flow cytometry, can be analyzed to determine additional physical properties of the particle.

摘要翻译： 流式细胞术概念被修改以使得能够使用光学散射数据获得的粒子的动态表征。 流入的颗粒将被引入样品体积。 收集并分析由样品体积中的颗粒散射的光。 将本文公开的概念与常规流式细胞术区分开来的是使用声源，其被设置为将声能引导到样本体积中。 当颗粒通过样品体积时，其响应于声能，引起颗粒散射的光的变化。 可以分析在常规流式细胞术中不测量的那些变化，以确定颗粒的附加物理性质。

公开(公告)号：US08415145B2

公开(公告)日：2013-04-09

申请号：US12892579

申请日：2010-09-28

申请人： Masakazu Fukuda ,  Hironori Kobayashi ,  Junyi Ding ,  Ryuichiro Ebi ,  Koki Tajima

发明人： Masakazu Fukuda ,  Hironori Kobayashi ,  Junyi Ding ,  Ryuichiro Ebi ,  Koki Tajima

IPC分类号： C12M1/12 ,  B01D35/00 ,  G01N1/34

CPC分类号： G01N1/34 ,  G01N15/0618 ,  G01N15/147 ,  G01N15/1475 ,  G01N35/1095 ,  G01N2015/1006 ,  G01N2015/1493 ,  G01N2035/00475

摘要： A cell processing apparatus 29 of the present invention includes a storage container 57 that can contain liquid L including a biological sample; a filter 60 that prevents a first cell C1 in the biological sample from passing therethrough and that allows a second cell C2 having a smaller diameter than that of the first cell C1 to pass therethrough; and a filtration cylinder 58 for separating, in the storage container 57 and via the filter 60, the liquid L into a first liquid L1 mainly including the first cell C1 and a second liquid L2 mainly including the second cell C2. A measurement target cell discriminated by the filter from the other cells can be easily collected.

摘要翻译： 本发明的细胞处理装置29包括能够容纳包含生物样品的液体L的储存容器57; 防止生物样品中的第一细胞C1通过并且允许具有比第一细胞C1的直径小的第二细胞C2通过的过滤器60; 以及用于将储存容器57和过滤器60中的液体L分离成主要包括第一单元C1的第一液体L1和主要包括第二单元C2的第二液体L2的过滤缸58。 可以容易地收集由过滤器与其他单元识别的测量对象单元。

公开(公告)号：US08358411B2

公开(公告)日：2013-01-22

申请号：US12642722

申请日：2009-12-18

申请人： John Y. Babico ,  Jian-Ping Jiang

发明人： John Y. Babico ,  Jian-Ping Jiang

IPC分类号： G01N15/02 ,  G01N21/00 ,  C12Q1/04 ,  C12M1/34

CPC分类号： G01N15/14 ,  C12Q1/04 ,  G01N15/1459 ,  G01N21/49 ,  G01N21/64 ,  G01N21/6486 ,  G01N2015/0065 ,  G01N2015/0088 ,  G01N2015/1493

摘要： A system for real-time sizing of fluid-borne particles is disclosed. The system further determines, in real time, whether the detected particles are biological or non-biological. As the fluid is being tested, it is exposed to a microbe collection filter which is cultured to determine the type of microbes present in the fluid being tested.

摘要翻译： 公开了一种用于实时定量流体颗粒的系统。 该系统实时地确定检测到的颗粒是生物还是非生物的。 当流体被测试时，其暴露于微生物收集过滤器，其被培养以确定存在于待测流体中的微生物的类型。

公开(公告)号：US08244022B2

公开(公告)日：2012-08-14

申请号：US12116012

申请日：2008-05-06

申请人： Kecheng Li ,  Dongbo Yan

发明人： Kecheng Li ,  Dongbo Yan

IPC分类号： G06K9/00 ,  G01B11/00

CPC分类号： G01N33/343 ,  G01N15/1463 ,  G01N2015/1493 ,  G01N2015/1495 ,  G01N2015/1497

摘要： A method for measuring a property of a fiber, such as flexibility, collapsibility and moment of inertia. A fiber is wetted and deformed in its wet state, and an optical section image of the deformed fiber is taken. A measurement is made on the image and the desired property is calculated using the measurement.

摘要翻译： 用于测量纤维的性质的方法，例如柔性，可收缩性和惯性矩。 纤维在其湿润状态下被润湿和变形，并且获得变形纤维的光学截面图像。 对图像进行测量，并使用测量计算所需的性质。

公开(公告)号：US20120202278A1

公开(公告)日：2012-08-09

申请号：US13447499

申请日：2012-04-16

申请人： Matthias Wagner ,  John Heanue

发明人： Matthias Wagner ,  John Heanue

IPC分类号： C12M1/42

CPC分类号： G01N15/1436 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0864 ,  B07C5/34 ,  C12N5/0612 ,  G01N15/14 ,  G01N15/1427 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/147 ,  G01N15/1484 ,  G01N21/1702 ,  G01N21/3563 ,  G01N29/2418 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2015/1497 ,  G01N2201/0612

摘要： This disclosure concerns a high-throughput cytometry system including a handling system that presents single cells to at least one quantum cascade laser (QCL) source. The QCL laser source is configured to deliver light successively to single cells over a time period in order to make successive measurements of induced resonant mid-infrared absorption by one or more analytes of the cells. The cytometry system also includes a mid-infrared detection facility that detects the successively transmitted mid-infrared wavelength light, wherein the transmitted mid-infrared wavelength light is used to identify a changing cell characteristic over the time period.

摘要翻译： 本公开涉及高通量细胞计数系统，其包括将单个细胞提供给至少一个量子级联激光（QCL）源的处理系统。 QCL激光源被配置为在一段时间内连续地将光递送到单个电池，以便连续测量由一个或多个细胞分析物引起的谐振中红外吸收。 细胞计数系统还包括中红外检测装置，其检测连续发送的中红外波长光，其中所发射的中红外波长光用于识别在该时间段内的变化的细胞特征。

公开(公告)号：US20120202277A1

公开(公告)日：2012-08-09

申请号：US13447464

申请日：2012-04-16

申请人： Matthias Wagner ,  John Heanue

发明人： Matthias Wagner ,  John Heanue

IPC分类号： C12M1/34

CPC分类号： G01N15/1436 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0864 ,  B07C5/34 ,  C12N5/0612 ,  G01N15/14 ,  G01N15/1427 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/147 ,  G01N15/1484 ,  G01N21/1702 ,  G01N21/3563 ,  G01N29/2418 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2015/1497 ,  G01N2201/0612

摘要： This disclosure concerns a minimally invasive cytometry system including a handling system that presents single cells to at least one QCL laser source. The QCL laser source is configured to deliver light to a cell within the cells in order to induce vibrational bond absorption in one or more analytes within the cell. The cytometry system also includes a detection facility that detects the mid-infrared wavelength light transmitted by the cell and identifies the cell as either cancerous or non-cancerous.

摘要翻译： 本公开涉及一种微创细胞计数系统，其包括向至少一个QCL激光源提供单个细胞的处理系统。 QCL激光源被配置为将光传送到细胞内的细胞，以便在细胞内的一种或多种分析物中诱导振动键吸收。 细胞计数系统还包括检测设备，其检测由细胞传播的中红外波长的光，并将细胞识别为癌或非癌细胞。

公开(公告)号：US08149401B2

公开(公告)日：2012-04-03

申请号：US12696673

申请日：2010-01-29

申请人： Jed Stevens ,  Gregory Sprenger

发明人： Jed Stevens ,  Gregory Sprenger

IPC分类号： G01N15/02

CPC分类号： G01N15/1429 ,  G01N15/1459 ,  G01N21/51 ,  G01N21/53 ,  G01N33/2847 ,  G01N2015/025 ,  G01N2015/1488 ,  G01N2015/1493 ,  G01N2021/4726

摘要： A method for detecting a presence of a particle in a fluid is disclosed. The method includes the steps of directing a beam of electromagnetic radiation into the transient fluid; providing a sensor to detect an intensity of the radiation after passing through at least a portion of the fluid; generating a data representing the intensity detected by the sensor; and analyzing the data based upon a statistical analysis to detect the presence of a particle in the fluid and determine whether the particle is water or a solid particle.

摘要翻译： 公开了一种用于检测流体中颗粒存在的方法。 该方法包括将电磁辐射束引入瞬态流体的步骤; 提供传感器以在通过至少一部分流体之后检测辐射的强度; 产生表示由传感器检测到的强度的数据; 并且基于统计分析来分析数据以检测流体中颗粒的存在并确定颗粒是水还是固体颗粒。

公开(公告)号：US08045162B2

公开(公告)日：2011-10-25

申请号：US12880523

申请日：2010-09-13

申请人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

发明人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

IPC分类号： G01N21/00

CPC分类号： G01N15/0205 ,  G01N15/0227 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/025 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2015/1493

摘要： A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, by utilizing the technique of laser rastering. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flow cell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

摘要翻译： 通过利用激光扫描技术提高流式细胞仪或使用流式细胞仪的血液分析仪的生产量，精度或精度以及吞吐量的方法。 激光扫描涉及通过血液分析仪中的流动样品流扫描激光束。 适用于实施本发明方法的装置包括一个包括光源的光学模块，一个扫描装置，一个透镜或透镜系统，一个流通池，检测器和过滤器; 以及包括前置放大器，模拟信号调节元件，模拟 - 数字转换器，现场可编程门阵列，数字信号处理元件和数据存储元件的电子模块。