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    White blood cell analysis system and method
    1.
    发明授权
    White blood cell analysis system and method 有权
    白细胞分析系统及方法

    公开(公告)号：US09091624B2

    公开(公告)日：2015-07-28

    申请号：US13456729

    申请日：2012-04-26

    申请人： Jiong Wu ,  Giacomo Vacca

    发明人： Jiong Wu ,  Giacomo Vacca

    IPC分类号： G01N15/14 ,  G01N15/10

    CPC分类号： G01N15/1434 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/49 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2021/6439

    摘要： Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WBCs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

    摘要翻译： 用于分析血液样品的系统和方法，更具体来说用于进行白细胞（WBC）差异分析。 系统和方法通过荧光染色和荧光触发策略筛选WBC。 因此，基本上消除了来自未分解的红细胞（RBC）和裂解的RBC的片段的干扰。 这些系统和方法还可以开发出相对温和的WBC试剂，适用于含有脆弱WBC的样品的测定。 在一个实施方案中，系统和方法包括：（a）用发射光谱对应于血液学仪器的激发源的专用细胞膜可渗透荧光染料染色血液样品; （b）使用荧光触发来筛选白细胞的血样; 和（c）使用（1）轴向光损失，（2）中角散射，（3）90°偏振侧散射，（4）90°去极化侧散射的测量，和（5）荧光发射来进行微分分析 。

    Basophil Analysis System and Method
    4.
    发明申请
    Basophil Analysis System and Method 有权
    嗜碱粒细胞分析系统和方法

    公开(公告)号：US20120282600A1

    公开(公告)日：2012-11-08

    申请号：US13456744

    申请日：2012-04-26

    申请人： Jiong Wu ,  Giacomo Vacca ,  Michael R. Buhl

    发明人： Jiong Wu ,  Giacomo Vacca ,  Michael R. Buhl

    IPC分类号： C12M1/42 ,  G01N21/64

    CPC分类号： G01N15/1434 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/49 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1486 ,  G01N2015/1488 ,  G01N2021/6439

    摘要： Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

    摘要翻译： 本文提供了用于分析血液样品的系统和方法，并且更具体地用于进行嗜碱性粒细胞分析。 在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; 然后（b）使用光散射和荧光发射的测量来区分嗜碱性粒细胞与其他WBC亚种群。 在一个实施方案中，系统和方法包括基于光散射和荧光测量的组合进行血液样品的嗜碱性粒细胞聚类分析。

    METHOD AND APPARATUS FOR RAPIDLY COUNTING AND IDENTIFYING BIOLOGICAL PARTICLES IN A FLOW STREAM
    6.
    发明申请
    METHOD AND APPARATUS FOR RAPIDLY COUNTING AND IDENTIFYING BIOLOGICAL PARTICLES IN A FLOW STREAM 失效
    方法和设备，用于快速计算和识别流动流中的生物颗粒

    公开(公告)号：US20080158561A1

    公开(公告)日：2008-07-03

    申请号：US11934277

    申请日：2007-11-02

    申请人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

    发明人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

    IPC分类号： G01N21/00

    CPC分类号： G01N15/0205 ,  G01N15/0227 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/025 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2015/1493

    摘要： A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, by utilizing the technique of laser rastering. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flow cell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

    摘要翻译： 通过利用激光扫描技术提高流式细胞仪或使用流式细胞仪的血液分析仪的生产量，精度或精度以及吞吐量的方法。 激光扫描涉及通过血液分析仪中的流动样品流扫描激光束。 适用于实施本发明方法的装置包括一个包括光源的光学模块，一个扫描装置，一个透镜或透镜系统，一个流通池，检测器和过滤器; 以及包括前置放大器，模拟信号调节元件，模拟 - 数字转换器，现场可编程门阵列，数字信号处理元件和数据存储元件的电子模块。

    Method and apparatus for rapidly counting and identifying biological particles in a flow stream
    7.
    发明授权
    Method and apparatus for rapidly counting and identifying biological particles in a flow stream 失效
    用于在流动流中快速计数和识别生物颗粒的方法和装置

    公开(公告)号：US07804594B2

    公开(公告)日：2010-09-28

    申请号：US11934277

    申请日：2007-11-02

    申请人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

    发明人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

    IPC分类号： G01N21/00

    CPC分类号： G01N15/0205 ,  G01N15/0227 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/025 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2015/1493

    摘要： A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, by utilizing the technique of laser rastering. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flow cell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

    摘要翻译： 通过利用激光扫描技术提高流式细胞仪或使用流式细胞仪的血液分析仪的生产量，精度或精度以及吞吐量的方法。 激光扫描涉及通过血液分析仪中的流动样品流扫描激光束。 适用于实施本发明方法的装置包括一个包括光源的光学模块，一个扫描装置，一个透镜或透镜系统，一个流通池，检测器和过滤器; 以及包括前置放大器，模拟信号调节元件，模拟 - 数字转换器，现场可编程门阵列，数字信号处理元件和数据存储元件的电子模块。

    Nucleated red blood cell analysis system and method
    8.
    发明授权
    Nucleated red blood cell analysis system and method 有权
    成核红细胞分析系统及方法

    公开(公告)号：US09103759B2

    公开(公告)日：2015-08-11

    申请号：US13456738

    申请日：2012-04-26

    申请人： Jiong Wu ,  Marilou Coleman ,  Michael R. Buhl ,  Giacomo Vacca ,  Emily H. Lin

    发明人： Jiong Wu ,  Marilou Coleman ,  Michael R. Buhl ,  Giacomo Vacca ,  Emily H. Lin

    IPC分类号： G01N15/14 ,  G01N15/10

    CPC分类号： G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N21/6428 ,  G01N33/49 ,  G01N33/80 ,  G01N2015/0069 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2021/6439

    摘要： Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

    摘要翻译： 用于分析血液样品的系统和方法，更具体地用于进行有核红细胞（nRBC）分析。 系统和方法通过荧光染色和荧光触发策略筛选血液样品，以鉴定血液样品中含有核的颗粒。 因此，基本上消除了来自未分解的红细胞（RBC）和裂解的RBC的片段的干扰。 这些系统和方法还能够开发适合于含有易碎白细胞（WBC）的样品的测定的较温和的试剂。 在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; （b）使用荧光触发器来筛选含有核的颗粒的血液样品; 和（c）使用光散射和荧光发射的测量来区分来自WBC的nRBC。

    Method and Apparatus for Detection, Analysis, and Collection of Rare Cellular Events
    10.
    发明申请
    Method and Apparatus for Detection, Analysis, and Collection of Rare Cellular Events 有权
    用于检测，分析和收集稀有细胞事件的方法和装置

    公开(公告)号：US20120295339A1

    公开(公告)日：2012-11-22

    申请号：US13473495

    申请日：2012-05-16

    申请人： Jiong Wu ,  Mahesh Junnarkar ,  Giacomo Vacca

    发明人： Jiong Wu ,  Mahesh Junnarkar ,  Giacomo Vacca

    IPC分类号： C12M1/42 ,  C12M1/36

    CPC分类号： G01N15/1459 ,  G01N15/1434 ,  G01N21/05 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/0346 ,  G01N2021/6482 ,  G01N2021/6493

    摘要： Systems and methods for the detection, analysis, and collection of rare cellular events, wherein rare cellular events are defined by events comprising less than 5% of a total number of cells in a sample. The systems and methods generally include: (1) a flow cell dimensioned so as to permit a flow of a sample through the flow cell at a flow rate greater than 300,000 cells per second; (2) a laser positioned to emit a laser beam directed to the flow cell; (3) one or more deflector components disposed between the laser and the flow cell, wherein the deflector component is configured to affect a position of the laser beam relative to the sample flow; (4) one or more fluorescence emission detectors; and (5) one or more processor configured to detect rare cellular events based on fluorescence emission from cell-binding surface markers introduced into the sample prior to the sample being flowed through the flow cell.

    摘要翻译： 用于检测，分析和收集罕见细胞事件的系统和方法，其中罕见的细胞事件由包含小于样品中总细胞数量的5％的事件定义。 系统和方法通常包括：（1）流动池，其尺寸设计成允许样品以大于每秒300,000个细胞的流速流过流动池; （2）定位成发射指向流动池的激光束的激光器; （3）设置在所述激光器和所述流动池之间的一个或多个偏转器部件，其中所述偏转器部件被配置为影响所述激光束相对于所述样品流的位置; （4）一个或多个荧光发射检测器; 以及（5）一个或多个处理器，被配置为基于在样品流过所述流动池之前引入到样品中的来自细胞结合表面标记的荧光发射来检测罕见的细胞事件。