公开(公告)号：US08715572B2

公开(公告)日：2014-05-06

申请号：US13473495

申请日：2012-05-16

申请人： Jiong Wu ,  Mahesh Junnarkar ,  Giacomo Vacca

发明人： Jiong Wu ,  Mahesh Junnarkar ,  Giacomo Vacca

IPC分类号： G01N21/64 ,  G01N15/06 ,  G06K9/00 ,  G01N15/02 ,  G01N21/49 ,  G01N21/85 ,  G01J3/30 ,  G01N21/00

CPC分类号： G01N15/1459 ,  G01N15/1434 ,  G01N21/05 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/0346 ,  G01N2021/6482 ,  G01N2021/6493

摘要： Systems and methods for the detection, analysis, and collection of rare cellular events, wherein rare cellular events are defined by events comprising less than 5% of a total number of cells in a sample. The systems and methods generally include: (1) a flow cell dimensioned so as to permit a flow of a sample through the flow cell at a flow rate greater than 300,000 cells per second; (2) a laser positioned to emit a laser beam directed to the flow cell; (3) one or more deflector components disposed between the laser and the flow cell, wherein the deflector component is configured to affect a position of the laser beam relative to the sample flow; (4) one or more fluorescence emission detectors; and (5) one or more processor configured to detect rare cellular events based on fluorescence emission from cell-binding surface markers introduced into the sample prior to the sample being flowed through the flow cell.

摘要翻译： 用于检测，分析和收集罕见细胞事件的系统和方法，其中罕见的细胞事件由包含小于样品中总细胞数量的5％的事件定义。 系统和方法通常包括：（1）流动池，其尺寸设计成允许样品以大于每秒300,000个细胞的流速流过流动池; （2）定位成发射指向流动池的激光束的激光器; （3）设置在所述激光器和所述流动池之间的一个或多个偏转器部件，其中所述偏转器部件被配置为影响所述激光束相对于所述样品流的位置; （4）一个或多个荧光发射检测器; 以及（5）一个或多个处理器，被配置为基于在样品流过所述流动池之前引入到样品中的来自细胞结合表面标记物的荧光发射来检测罕见的细胞事件。

公开(公告)号：US20120295339A1

公开(公告)日：2012-11-22

申请号：US13473495

申请日：2012-05-16

申请人： Jiong Wu ,  Mahesh Junnarkar ,  Giacomo Vacca

发明人： Jiong Wu ,  Mahesh Junnarkar ,  Giacomo Vacca

IPC分类号： C12M1/42 ,  C12M1/36

CPC分类号： G01N15/1459 ,  G01N15/1434 ,  G01N21/05 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/0346 ,  G01N2021/6482 ,  G01N2021/6493

摘要： Systems and methods for the detection, analysis, and collection of rare cellular events, wherein rare cellular events are defined by events comprising less than 5% of a total number of cells in a sample. The systems and methods generally include: (1) a flow cell dimensioned so as to permit a flow of a sample through the flow cell at a flow rate greater than 300,000 cells per second; (2) a laser positioned to emit a laser beam directed to the flow cell; (3) one or more deflector components disposed between the laser and the flow cell, wherein the deflector component is configured to affect a position of the laser beam relative to the sample flow; (4) one or more fluorescence emission detectors; and (5) one or more processor configured to detect rare cellular events based on fluorescence emission from cell-binding surface markers introduced into the sample prior to the sample being flowed through the flow cell.

摘要翻译： 用于检测，分析和收集罕见细胞事件的系统和方法，其中罕见的细胞事件由包含小于样品中总细胞数量的5％的事件定义。 系统和方法通常包括：（1）流动池，其尺寸设计成允许样品以大于每秒300,000个细胞的流速流过流动池; （2）定位成发射指向流动池的激光束的激光器; （3）设置在所述激光器和所述流动池之间的一个或多个偏转器部件，其中所述偏转器部件被配置为影响所述激光束相对于所述样品流的位置; （4）一个或多个荧光发射检测器; 以及（5）一个或多个处理器，被配置为基于在样品流过所述流动池之前引入到样品中的来自细胞结合表面标记的荧光发射来检测罕见的细胞事件。

公开(公告)号：US20050063631A1

公开(公告)日：2005-03-24

申请号：US10669206

申请日：2003-09-24

申请人： Mahesh Junnarkar ,  Anirban Bandyopadhyay

发明人： Mahesh Junnarkar ,  Anirban Bandyopadhyay

IPC分类号： G02B6/34 ,  G02F1/01 ,  G02F1/295 ,  G02B6/26 ,  G02B6/42

CPC分类号： G02B6/12011 ,  G02B6/12028 ,  G02F1/0147

摘要： A temperature gradient may be provided across an array of waveguides in an arrayed waveguide grating. As a result, temperature tuning may be provided to adjust the characteristics of the arrayed waveguide grating. For example, the array of waveguides positioned on one side of a planar light wave circuit may be heated by a similarly configured array of heaters on the opposite side of the circuit. In some cases the number of heaters may be less than the number of arrayed waveguides. Also, each of the heaters in one embodiment may be selectively actuatable.

摘要翻译： 可以在阵列波导光栅中的波导阵列之间提供温度梯度。 结果，可以提供温度调谐以调整阵列波导光栅的特性。 例如，位于平面光波电路一侧的波导阵列可以通过类似配置的电路相对侧的加热器阵列加热。 在一些情况下，加热器的数量可能小于阵列波导的数量。 而且，一个实施例中的每一个加热器都可以选择性地致动。

公开(公告)号：US06983086B2

公开(公告)日：2006-01-03

申请号：US10465210

申请日：2003-06-19

申请人： Behzad Fardi ,  Farshid Adibi ,  Chaoyang Li ,  Anirban Bandyopadhyay ,  Mahesh Junnarkar

发明人： Behzad Fardi ,  Farshid Adibi ,  Chaoyang Li ,  Anirban Bandyopadhyay ,  Mahesh Junnarkar

IPC分类号： G02B6/12

CPC分类号： G02F1/025 ,  G02F1/0147

摘要： A thermo-optic device may be formed with trenches that undercut the substrate beneath the thermo-optic device. Through the removal of the underlying substrate, the heat dissipation of the thermo-optic device may be reduced. This may reduce the thermal budget of the device, reducing the power requirements for operating the device in some embodiments.

摘要翻译： 热光器件可以形成有在热光器件下面切割衬底的沟槽。 通过去除下面的衬底，可以减少热光器件的散热。 这可能降低装置的热量预算，在一些实施例中减少了操作装置的功率要求。

公开(公告)号：US20050024627A1

公开(公告)日：2005-02-03

申请号：US10631538

申请日：2003-07-31

申请人： Mahesh Junnarkar ,  Bidhan Chaudhuri

发明人： Mahesh Junnarkar ,  Bidhan Chaudhuri

IPC分类号： G01M11/00 ,  G01N21/00

CPC分类号： G01M11/35

摘要： A waveguide under test can be exposed to a light signal whose polarization rotates between the vertical and horizontal polarizations. The intensity detected at a photodetector can be separated into AC and DC components. The AC components may be utilized to derive a characteristics which is indicative of birefringence of the waveguide. If the light signal is scanned over the waveguide under test, a measure of the birefringence at each position along the waveguide may be determined.

摘要翻译： 受测试的波导可以暴露于其垂直和水平极化之间偏振旋转的光信号。 在光电检测器处检测的强度可以分为AC和DC分量。 可以使用AC分量来导出指示波导的双折射的特性。 如果光信号在被测波导上扫描，则可以确定沿着波导的每个位置处的双折射的测量。