公开(公告)号：US09957562B2

公开(公告)日：2018-05-01

申请号：US14002125

申请日：2012-01-24

Applicant: Koshin Hamasaki ,  Toshiro Saito ,  Takayuki Obara

Inventor： Koshin Hamasaki ,  Toshiro Saito ,  Takayuki Obara

IPC: C12Q1/68 ,  G01N21/64 ,  C40B40/06

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6874 ,  C40B40/06 ,  G01N21/6452 ,  G01N21/648 ,  G01N2021/6439 ,  C12Q2521/543 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2565/513 ,  C12Q2523/308

Abstract: In the conventional nucleic acid analysis devices and nucleic acid analyzers, there was no technique available for sequencing a single nucleic acid molecule easily and highly efficiently. The present invention enabled a highly efficient single molecule immobilization of nucleic acid with good reproductivity in a short time at a low price by providing small metallic bonding pads at predetermined positions on a support substrate, firmly fixing a hydrophobic linker on the bonding pads, and bonding on to the linker bulky microparticles onto which a single molecule of a nucleic acid sample fragment is immobilized. According to the present invention, in the nucleic acid analysis device which uses a nucleic acid analyzer, the nucleotide read length can be extended and many types of nucleic acid molecule to be analyzed can be analyzed at one time.

公开(公告)号：US20180114316A1

公开(公告)日：2018-04-26

申请号：US15789414

申请日：2017-10-20

Applicant: University of Florida Research Foundation, Inc.

Inventor： Tanmay P. Lele ,  Wallace Gregory Sawyer

IPC: G06T7/00 ,  B01L3/00 ,  C40B40/06 ,  C12Q1/68 ,  G01N33/50

CPC classification number: G06T7/0012 ,  B01L3/50273 ,  B01L2400/0481 ,  C12N15/1003 ,  C12Q1/6841 ,  C12Q1/6851 ,  C40B40/06 ,  G01N33/50 ,  G06T2207/30024 ,  C12Q2527/109 ,  C12Q2523/303

Abstract: Embodiments of the present disclosure provide for methods and systems for preparing chromosomal spread for a selected cell so that chromosomal spreads and/or translocations can be correlated with the selected cell.

公开(公告)号：US09927434B2

公开(公告)日：2018-03-27

申请号：US12930977

申请日：2011-01-20

Applicant: John Cooper

Inventor： John Cooper

IPC: C09D4/00 ,  C40B40/06 ,  G01N33/543

CPC classification number: G01N33/5438

Abstract: Disclosed herein is a multiplex microarray having serially attached non-functionalized biomolecules attached to a polymer coating covering each electrode of an array of electrodes for assays and a method of making the multiplex microarray. The method comprises serially blocking the electrodes of the microarray with a blocking protein, electropolymerizing pyrrole or a functionalized pyrrole on the electrodes where the biomolecule is not present during polymerization, exposing the microarray to a biomolecular solution containing a non-functionalized biomolecule for attachment to the polymer coating, and then repeating the steps to form the multiplex microarray.

公开(公告)号：US09920363B2

公开(公告)日：2018-03-20

申请号：US14358674

申请日：2012-11-15

Applicant: BGI TECH SOLUTIONS CO., LTD.

Inventor： Fei Gao ,  Junwen Wang ,  Tong Wang ,  Hui Jiang ,  Jinghua Wu ,  Honglong Wu

IPC: C12Q1/68 ,  C12N15/10 ,  C40B40/06 ,  C40B40/08 ,  C40B50/06

CPC classification number: C12Q1/6869 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6876 ,  C40B40/06 ,  C40B40/08 ,  C40B50/06 ,  C12Q2523/125 ,  C12Q2525/117 ,  C12Q2525/191 ,  C12Q2535/122

Abstract: The present invention provides a method for constructing a high-throughput sequencing library, which comprises: fragmenting genomic DNA; end-repairing the DNA fragments; adding a base A to the 3′ end of the end-repaired DNA fragments; connecting the DNA fragments having cohesive end A with a methylated adapter; carrying out hybrid capture on the connection products by using specific probes to obtain object fragments; treating the object fragments with bisulfite, to convert non-methylated cytosines to uracils; PCR amplifying the converted object fragments; and separating and purifying the amplification products, wherein the amplification products constitute the high-throughput sequencing library. The present invention also provides a method and an apparatus for identifying methylation information in specified genome regions of a sample.

公开(公告)号：US20170354967A1

公开(公告)日：2017-12-14

申请号：US15679111

申请日：2017-08-16

Applicant: CapitalBio Corporation ,  Tsinghua University

Inventor： Shengce TAO ,  Jing CHENG ,  Xuemei MA ,  Qiong ZHANG ,  Yuxiang ZHOU

IPC: B01L3/00 ,  C12Q1/68 ,  C40B40/06 ,  B01L7/00 ,  C40B60/14

CPC classification number: B01L3/508 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00722 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/163 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  C12Q2547/101 ,  C12Q2531/131

Abstract: This invention relates generally to the field of nucleic acid detection. In particular, the invention provides a lab-on-chip system for analyzing a nucleic acid, which system comprises, inter alia, controllably closed space, and a target nucleic acid can be prepared and/or amplified, and hybridized to a nucleic acid probe, and the hybridization signal can be acquired if desirable, in the controllably closed space without any material exchange between the controllably closed space and the outside environment. Methods for analyzing a nucleic acid using the lab-on-chip system is also provided.

公开(公告)号：US09771621B2

公开(公告)日：2017-09-26

申请号：US15212589

申请日：2016-07-18

Applicant: BIOMERIEUX

Inventor： Xun Ye ,  Fei Wu ,  Qinghua Xu ,  Xia Meng ,  Bruno Mougin ,  Fang Liu

IPC: C07H21/04 ,  C12Q1/68 ,  C40B40/06

CPC classification number: C12Q1/6886 ,  C12Q1/6837 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158 ,  C40B40/06

Abstract: The present invention relates to method and kit for performing a colorectal cancer assay. Especially a method including extracting total RNA from a peripheral blood sample obtained from a patient suspected of having or having colorectal cancer; contacting the total RNA, or cDNA or cRNA obtained from the total RNA, with one or more reagents specific for at least one target gene and no more than 100 target genes; and measuring the expression level of the at least one target gene and no more than 100 target genes. The at least one target gene and no more than 100 target genes includes one or more members selected from the group consisting of the KLRB1, KLRC2, KLRC3, KLRD1, KLRK1, CD247, RRAS2, SH2D1B, LCK, MRPS6, SPRY4, CYBB, DUSP2, PDE4D, SH2D2A, GZMB, INSR, ITGAM, VCAN, CD163, P2RY10, CD226, MRPL10, ITPRIPL2, CD2, and NUDT16 genes.

公开(公告)号：US09713625B2

公开(公告)日：2017-07-25

申请号：US14989432

申请日：2016-01-06

Applicant: The Ohio State University

Inventor： Carlo M. Croce ,  Andrea Vecchione

IPC: C12Q1/68 ,  C40B30/04 ,  C40B40/06 ,  C07H21/02 ,  C07H21/04 ,  A61K31/711 ,  A61K31/282 ,  A61K31/337 ,  A61K31/7105 ,  A61K45/06

CPC classification number: A61K31/711 ,  A61K31/282 ,  A61K31/337 ,  A61K31/7105 ,  A61K45/06 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/178

Abstract: Described herein are methods for diagnosing ovarian cancer. In particular, certain microRNAs are useful to the response to chemotherapy of ovarian cancer patients.

公开(公告)号：US09637793B2

公开(公告)日：2017-05-02

申请号：US13518801

申请日：2009-12-24

Applicant: Chenyu Zhang ,  Rui Liu ,  Cheng Wang ,  Yi Bai ,  Chunni Zhang ,  Ke Zeng

Inventor： Chenyu Zhang ,  Rui Liu ,  Cheng Wang ,  Yi Bai ,  Chunni Zhang ,  Ke Zeng

IPC: C12Q1/68 ,  C40B40/06

CPC classification number: C12Q1/6886 ,  C12Q1/6813 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/178 ,  C40B40/06

Abstract: The present invention provides microRNAs for assessing the status of pancreatic cancer in a subject, and provides methods, kits, and biochips for detecting said microRNAs.

公开(公告)号：US09632033B2

公开(公告)日：2017-04-25

申请号：US14746135

申请日：2015-06-22

Applicant: International Business Machines Corporation

Inventor： Venkat K. Balagurusamy ,  Stephen J. Heisig ,  Gong Su

IPC: C12Q1/68 ,  C12M1/34 ,  C40B40/06 ,  G01N21/75 ,  G02B21/36 ,  G02B21/00 ,  G01N21/64 ,  G06K9/00 ,  G06T7/20

CPC classification number: G01N21/75 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2201/0612 ,  G01N2201/062 ,  G02B21/0008 ,  G02B21/367 ,  G06K9/0014 ,  G06T7/246 ,  G06T7/248 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30072

Abstract: A technique relates to determining a presence of a known nucleic acid sequence of a known molecule in a sample. A sample surface is coated with a select segment of the known molecule. Beads are coated with a first molecule. A targeted nucleic acid sequence is attached to a second molecule that binds to the first molecule, such that the targeted nucleic acid sequence is attached to the beads via first and second molecules. The sample is placed on the sample surface. The sample includes the liquid medium, beads, and targeted nucleic acid sequence being tested. Brownian motion of beads is monitored to determine whether the Brownian motion of the beads is restricted or not restricted. When the Brownian motion of beads is restricted, the presence of the known nucleic acid sequence is in the sample, thus indicating that the targeted nucleic acid sequence is the known nucleic acid sequence.

公开(公告)号：US09624252B2

公开(公告)日：2017-04-18

申请号：US14556493

申请日：2014-12-01

Applicant: Omega Bio-Tek, Inc.

Inventor： Qi Guo

IPC: C12Q1/68 ,  C12M1/00 ,  C12P19/34 ,  C07H21/02 ,  C07H1/06 ,  C40B40/06 ,  G01N31/22 ,  C12N15/10 ,  C07H21/04

CPC classification number: C07H1/06 ,  C07H21/04 ,  C12N15/1006

Abstract: The invention provides methods and kits for nucleic acid purification and fragment selection and recovery. By adjusting salt concentrations and/or pH of the binding buffers, only nucleic acid fragments with desired size ranges are able to reversibly and non-specifically bind to a solid surface in certain salt concentrations and/or pH conditions, and can be subsequently eluted and/or recovered from the solid surface in water and/or a low salt eluting buffer.