公开(公告)号：US20100120636A1

公开(公告)日：2010-05-13

申请号：US12683272

申请日：2010-01-06

Applicant: Andrew Richard Russell Prewer

Inventor： Andrew Richard Russell Prewer

IPC: C40B50/14 ,  C40B60/08

CPC classification number: B01J19/24 ,  B01J19/0006 ,  B01J19/0046 ,  B01J2219/00058 ,  B01J2219/00164 ,  B01J2219/00274 ,  B01J2219/00331 ,  B01J2219/00454 ,  B01J2219/00497 ,  B01J2219/00515 ,  B01J2219/00518 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00655 ,  B01J2219/00675 ,  B01J2219/00686 ,  B01J2219/00725 ,  B01J2219/00871 ,  B01J2219/24 ,  B82Y30/00 ,  C07K1/042 ,  C07K1/045 ,  C07K1/047 ,  C40B60/14

Abstract: An apparatus and system for contacting a mobile elongate solid phase, e.g. a ribbon with a flowing fluid phase, and a method for using the same in, for example solid phase synthesis. A particular apparatus comprises (i) a conduit which is of circular or non-circular transverse cross section and which defines a lumen to contain both the flowing fluid phase and the mobile elongate solid phase; (ii) fluid phase ports in communication with the lumen to allow the fluid phase to enter the lumen, flow through it and exit it; and (iii) solid phase ports in communication with the lumen to allow the mobile solid phase to enter the lumen, move through it and exit it, the apparatus being adapted to prevent fluid egress from its interior through the solid phase ports.Also provided is a continuous process for synthesising and screening molecules, comprising passing an elongate solid phase through successive treatment stations where different stages of a solid phase synthesis are performed, and screening the synthesised molecules for activity.

Abstract translation: 一种用于接触移动细长固相的装置和系统，例如， 具有流动相的流动相的带，以及在固相合成中使用该带的方法。 特定装置包括（i）具有圆形或非圆形横截面并且限定内腔以容纳流动流体相和移动细长固体相的管道; （ii）与内腔连通以允许流体相进入管腔的流体相口，流过其并将其排出; 和（iii）与内腔连通的固相端口，以允许移动固相进入管腔，移动通过其并将其排出，该装置适于防止流体从其内部通过固相端口流出。 还提供了用于合成和筛选分子的连续方法，包括使细长的固相通过连续的处理站，其中进行固相合成的不同阶段，并筛选合成的分子的活性。

公开(公告)号：US20100093561A1

公开(公告)日：2010-04-15

申请号：US12285629

申请日：2008-10-09

Applicant: Juha Rantala ,  Petri Saviranta ,  Olli Kallioniemi

Inventor： Juha Rantala ,  Petri Saviranta ,  Olli Kallioniemi

IPC: C40B30/06 ,  C40B50/14 ,  C40B40/02

CPC classification number: G01N33/5032

Abstract: The present invention relates to the fields of life sciences and biological processes. Specifically, the invention relates to microarrays and live cell based screening and molecular analysis. More specifically, the present invention relates to novel methods for the screening of the effects of a test compound on cells, for molecular analysis of the cells and for producing a microarray. The present invention also relates to cell arrays and the use of arrays for molecular analysis of the cells or for the screening of agents.

Abstract translation: 本发明涉及生命科学和生物过程领域。 具体地，本发明涉及微阵列和基于活细胞的筛选和分子分析。 更具体地，本发明涉及用于筛选测试化合物对细胞的影响，细胞分子分析和产生微阵列的新方法。 本发明还涉及细胞阵列和阵列用于细胞分子分析或用于筛选试剂的用途。

公开(公告)号：US20100075865A1

公开(公告)日：2010-03-25

申请号：US12376040

申请日：2007-08-03

Applicant: Dieter Trau ,  Wen-Tso Liu ,  Kian Kok Johnson Ng

Inventor： Dieter Trau ,  Wen-Tso Liu ,  Kian Kok Johnson Ng

IPC: C40B30/04 ,  C40B50/14 ,  C40B50/02 ,  C40B40/00

CPC classification number: G01N33/54373 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/0054 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/0074 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6883 ,  C40B20/02 ,  C40B30/04 ,  C40B40/04 ,  C40B50/16 ,  C40B50/18 ,  C40B60/14 ,  G01N21/6452 ,  G01N33/54313 ,  G01N33/545 ,  G06T5/50 ,  G06T7/0008 ,  G06T7/33 ,  C12Q2537/149 ,  C12Q2563/149 ,  C12Q2565/513 ,  C12Q2565/619

Abstract: A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray.

Abstract translation: 制作微阵列的过程。 该方法包括在具有至少一个基准的基底上沉积一群微珠的步骤。 群体由至少两个亚群组成，优选多个亚群，每个亚群包含能够与至少一种目标分析物特异性结合的已知活性剂。 所述亚群是以彼此不连续的时间顺序沉积的。 该方法还包括在每个亚群沉积之后制作衬底的图像的步骤。 然后使用基准作为参考来比较图像，从而基于每个图像之间的差异来确定每个微珠的位置并识别亚群及其已知的活性剂。 还在用于使用微阵列的系统中公开。

公开(公告)号：US20100056382A1

公开(公告)日：2010-03-04

申请号：US12474747

申请日：2009-05-29

Applicant: Michael R. Sussman ,  Francesco Cerrina ,  Kathryn Richmond ,  Jamison Wolfer

Inventor： Michael R. Sussman ,  Francesco Cerrina ,  Kathryn Richmond ,  Jamison Wolfer

IPC: C40B30/00 ,  C40B40/06 ,  C40B50/14

CPC classification number: B01J19/0046 ,  B01J2219/00436 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00722 ,  C12Q1/6837 ,  C40B50/14

Abstract: The present invention provides novel arrays of oligonucleotide probes immobilized on a solid support in the form of a chip (millichip), which can be used for rapid and inexpensive analysis of nucleic acids. The arrays can have a plurality of different oligonucleotide probes that can provide for whole genome gene expression analysis. The millichip can be used for analysis of both RNA and DNA.

Abstract translation: 本发明提供固定在芯片（millichip）形式的固体支持物上的寡核苷酸探针的新阵列，其可用于快速且廉价地分析核酸。 阵列可以具有多个可提供全基因组基因表达分析的不同寡核苷酸探针。 millichip可用于RNA和DNA的分析。

公开(公告)号：US20100048421A1

公开(公告)日：2010-02-25

申请号：US12540027

申请日：2009-08-12

Applicant: Si-Ping HAN ,  Hareem Maune ,  Robert D. Barish ,  William A. Goddard, III

Inventor： Si-Ping HAN ,  Hareem Maune ,  Robert D. Barish ,  William A. Goddard, III

IPC: C40B40/04 ,  C07H21/00 ,  C40B50/14

CPC classification number: B82B1/00 ,  B82Y30/00

Abstract: The disclosure relates to nanotube composite structures and related methods and systems. In particular, structures, methods and systems are provided herein to allow for precise, tunable separation between nanomaterials such as carbon nanotubes.

Abstract translation: 本公开涉及纳米管复合结构及相关方法和系统。 特别地，本文提供了结构，方法和系统，以允许诸如碳纳米管之类的纳米材料之间的精确的，可调节的分离。

公开(公告)号：US20100029508A1

公开(公告)日：2010-02-04

申请号：US12261406

申请日：2008-10-30

Applicant: Robert H. Austin ,  Zhaoning Yu ,  Jonas O. Tegenfeldt ,  Stephen Y. Chou ,  Han Cao

Inventor： Robert H. Austin ,  Zhaoning Yu ,  Jonas O. Tegenfeldt ,  Stephen Y. Chou ,  Han Cao

IPC: C40B40/06 ,  C40B40/00 ,  C40B50/14 ,  C40B60/12 ,  C40B30/00

CPC classification number: C12Q1/6869 ,  B01J2219/00274 ,  B01L3/502707 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/0861 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/086 ,  B81B1/002 ,  B81B2201/058 ,  B81B2203/0338 ,  B81C1/00071 ,  B82Y30/00 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/48721 ,  Y10S977/704 ,  Y10S977/88 ,  Y10S977/883

Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

Abstract translation: 公开了能够实现高通量大分子分析的纳米通道阵列。 还公开了制备纳米通道阵列和纳米流体芯片的方法。 还公开了分析大分子的方法，例如基因组DNA的全部链，以及用于实施这些方法的系统。

公开(公告)号：US20090312197A1

公开(公告)日：2009-12-17

申请号：US12300235

申请日：2007-12-14

Applicant: Hyunil Lee ,  Hee Kyung Park ,  Bong Ho Um ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Hye Yeon Kim

Inventor： Hyunil Lee ,  Hee Kyung Park ,  Bong Ho Um ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Hye Yeon Kim

IPC: C40B40/08 ,  C07K9/00 ,  C07K1/00 ,  C40B50/14

CPC classification number: C07K14/003 ,  A61K47/641 ,  Y10S977/754

Abstract: The present invention relates to a peptide nucleic acid (PNA) conjugated with multi-amine linkers, and a method to prepare the same and utilization thereof. More specifically, the method is characterized by conjugating monomers having multi-amine functionality sequentially at a PNA terminal, and effectively immobilizing the PNA conjugated with multi-amine linkers on a solid surface. A PNA array prepared using the PNA conjugated with multi-amine linkers exhibits improved sensitivity and specificity of signals for detecting target nucleic acids as compared to a PNA array using PNA probes having only one amine group. The PNA conjugated with multi-amine linkers can be utilized in nucleic acid detecting devices or kits for gene diagnosis such as PNA microarrays, PNA chips, PNA field-effect transistors and impedance detectors.

Abstract translation: 本发明涉及与多胺接头共轭的肽核酸（PNA）及其制备方法及其应用。 更具体地说，该方法的特征在于在PNA末端连续连接具有多胺官能团的单体，并有效地将与多胺接头共轭的PNA固定在固体表面上。 使用与多胺接头共轭的PNA制备的PNA阵列与使用仅具有一个胺基团的PNA探针的PNA阵列相比，显示出用于检测靶核酸的信号的改善的灵敏度和特异性。 与多胺接头结合的PNA可用于核酸检测装置或用于基因诊断的试剂盒，例如PNA微阵列，PNA芯片，PNA场效应晶体管和阻抗检测器。

公开(公告)号：US20090305908A1

公开(公告)日：2009-12-10

申请号：US12482767

申请日：2009-06-11

Applicant: Eric S. Nordman ,  Mark F. Oldham ,  Timothy Woudenberg

Inventor： Eric S. Nordman ,  Mark F. Oldham ,  Timothy Woudenberg

IPC: C40B50/18 ,  C40B50/14

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/6486 ,  G01N2021/6441 ,  C12Q2563/107

Abstract: A method for preferentially localizing desired molecules within an optical confinement disposed upon a substrate is disclosed. The desired molecules are deposited over the surface of the substrate. The desired molecules that are not within the optical confinement are selectively removed from the surface of the substrate.

Abstract translation: 公开了一种用于在设置在基板上的光限制内优先定位所需分子的方法。 期望的分子沉积在衬底的表面上。 不在光学限制范围内的期望分子被选择性地从衬底的表面去除。

公开(公告)号：US20090275482A1

公开(公告)日：2009-11-05

申请号：US12279570

申请日：2007-01-26

Applicant: Jing Gao ,  B. Shane Giles ,  Peter G. Webb ,  Douglas N. Roberts

Inventor： Jing Gao ,  B. Shane Giles ,  Peter G. Webb ,  Douglas N. Roberts

IPC: C40B30/04 ,  C40B50/14 ,  C40B40/08 ,  G06F19/00

CPC classification number: G06F19/20

Abstract: A method of selecting a set of normalization probes for use on a comparative genome hybridization array is provided. In certain embodiments, the method includes: a) selecting a first region of a genome to be evaluated by comparative genome hybridization to produce data; b) selecting a second region of the genome for normalization of the data, and c) selecting from a set of candidate probes a sub-set of normalization probes that detect the second region.

Abstract translation: 提供了选择用于比较基因组杂交阵列的一组归一化探针的方法。 在某些实施方案中，该方法包括：a）通过比较基因组杂交来选择待评估的基因组的第一区域以产生数据; b）选择所述基因组的第二区域以使所述数据进行归一化，以及c）从一组候选探针中选择检测所述第二区域的归一化探针的子集。

公开(公告)号：US20090264317A1

公开(公告)日：2009-10-22

申请号：US12421259

申请日：2009-04-09

Applicant: Yuval Ofir ,  Vincent Martin Rotello ,  Brian John Jordan ,  Kenneth Raymond Carter

Inventor： Yuval Ofir ,  Vincent Martin Rotello ,  Brian John Jordan ,  Kenneth Raymond Carter

IPC: C40B50/14 ,  C40B30/00 ,  C40B40/04 ,  C40B40/06 ,  C40B40/10 ,  B29C35/08 ,  B32B3/00

CPC classification number: G03F7/0002 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y40/00 ,  H05K3/102 ,  H05K3/389 ,  H05K2201/0257 ,  H05K2203/0108 ,  H05K2203/0522 ,  Y10T428/24802

Abstract: Disclosed is a method comprising disposing a functionalized patternable material on a substrate, wherein the functionalized patternable material comprises a first click chemical moiety; patterning the functionalized patternable material; and reacting the first click chemical moiety with a complementary reactant to form an functionalized patterned surface, the complementary reactant comprising a second click chemical moiety that reacts with the first click chemical moiety; the complementary reactant comprising an functional group.

Abstract translation: 公开了一种方法，包括将功能化的可图案材料设置在基底上，其中所述官能化可图案材料包括第一点击化学部分; 图案化功能化图案化材料; 并且使所述第一点击化学部分与互补反应物反应以形成官能化图案化表面，所述互补反应物包含与所述第一点击化学部分反应的第二点击化学部分; 包含官能团的互补反应物。