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公开(公告)号：US20190153517A1
公开(公告)日：2019-05-23
申请号：US16261532
申请日：2019-01-29
Applicant: General Electric Company
Inventor： Bing Li , David Roger Moore , William Christopher Alberts , John Richard Nelson
IPC: C12Q1/6806 , G01N33/543
Abstract: An electrospinning approach is disclosed for generating a dissolvable formulation of a reagent of interest in a nanoscale fiber medium. In one embodiment, the nanoscale fibers can incorporate and stabilize biological agents of interest, such as for storage at room temperature for extended periods. In one implementation, the fibers can be produced in a continuous manner and dissolve rapidly.
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公开(公告)号：US20180223349A1
公开(公告)日：2018-08-09
申请号：US15949061
申请日：2018-04-09
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Ryan Charles Heller , Erik Leeming Kvam , John Richard Nelson
IPC: C12Q1/6844 , C12Q1/6869
CPC classification number: C12Q1/6844 , C12Q1/6869 , C12Q2521/501 , C12Q2525/179 , C12Q2531/125
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.
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公开(公告)号：US10030241B2
公开(公告)日：2018-07-24
申请号：US15150806
申请日：2016-05-10
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Patrick McCoy Spooner , Peter James Tatnell , Jeffrey Kenneth Horton , John Richard Nelson , Michael John Gerdes , Suzana Kiel , Ralf Lenigk , Alexander Schenk , Wei Sun
IPC: C12N15/10 , G01N33/53 , C12Q1/6804 , G01N27/447 , G01N33/68 , C12Q1/6888
Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.
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公开(公告)号：US10000742B2
公开(公告)日：2018-06-19
申请号：US14945483
申请日：2015-11-19
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Erik Leeming Kvam , Robert Scott Duthie , John Richard Nelson
CPC classification number: C12N7/00 , B01L3/508 , B01L2200/082 , C12N15/1017 , C12N2760/14111 , C12N2760/14151 , C12N2760/14163 , C12Q1/6806 , C12Q1/70 , C12Q1/701 , C12Q2523/113 , C12Q2523/308 , C12Q2527/125
Abstract: The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.
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公开(公告)号：US09957548B2
公开(公告)日：2018-05-01
申请号：US14672358
申请日：2015-03-30
Applicant: General Electric Company
Inventor： Michael John Gerdes , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk
CPC classification number: C12Q1/6804 , C12N15/1003 , C12Q2522/101 , G01N33/689
Abstract: A method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample is disclosed. The method includes a step of contacting a lysis solution to the sample that includes at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate includes a protamine-DNA complex. The method further includes applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The method further includes capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.
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Patent Agency Ranking
公开(公告)号：US09828599B2
公开(公告)日：2017-11-28
申请号：US14030386
申请日：2013-09-18
Applicant: General Electric Company
Inventor： Nandini Nagraj , Radislav Alexandrovich Potyrailo , Andrew David Pris , John Richard Nelson
CPC classification number: C12N15/1034 , C12N15/1048 , C12Q1/6811 , C40B30/04 , G01N33/53 , G01N33/5308 , H04N1/32309 , H04N1/3232 , H04N1/32352 , H04N1/40 , H04N1/60 , H04N2201/0094 , H04N2201/3233 , H04N2201/327 , H04N2201/3271 , H04N2209/00 , C12Q2525/205 , C12Q2531/125 , C12Q2537/162 , C12Q2565/107
Abstract: Methods for selecting a binding-element are provided. The method comprised of different steps. A first mixture is formed using at least one target molecule and a plurality of oligomers, followed by incubating the first mixture to form a second mixture comprising at least one target-bound oligomer and at least one target-unbound oligomer. Then a first accelerator is added to cleave the target-unbound oligomer and the target-bound oligomer is separated from the target molecule. This is followed by addition of a second accelerator for ligation, and a third accelerator for amplification followed by sequencing and post sequence analysis to select the binding-element.
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公开(公告)号：US09765375B2
公开(公告)日：2017-09-19
申请号：US13929976
申请日：2013-06-28
Applicant: General Electric Company
Inventor： Anthony John Murray , John Richard Nelson
IPC: C12P19/34 , C12Q1/68 , C12N15/115 , C12N15/11
CPC classification number: C12P19/34 , C12N15/111 , C12N15/115 , C12N2310/16 , C12N2320/10
Abstract: Methods for developing a binding-element are provided. A mixture comprising a target molecule, a plurality of oligonucleotides and a ligase is provided, followed by binding the oligonucleotides to the target molecule to form an oligonucleotides-target molecule complex. The oligonucleotides bound to the target molecule are ligated to form the binding-element. The binding-elements are separated from the mixture.
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公开(公告)号：US20170175105A1
公开(公告)日：2017-06-22
申请号：US15453719
申请日：2017-03-08
Applicant: General Electric Company
Inventor： John Richard Nelson , Wei Gao , Christopher Michael Puleo , Todd Frederick Miller , Christine Lynne Pitner , David Andrew Shoudy , Alex David Corwin
CPC classification number: C12N15/1013 , B01L3/502 , B01L2200/025 , B01L2200/0689 , B01L2300/06 , B01L2300/0654 , B01L2300/0861 , C12Q1/6806 , G01N1/02 , G01N1/10 , G01N1/40 , G01N2001/028 , G01N2001/4038
Abstract: A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base, a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.
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公开(公告)号：US20160289666A1
公开(公告)日：2016-10-06
申请号：US15150806
申请日：2016-05-10
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Patrick McCoy Spooner , Peter James Tatnell , Jeffrey Kenneth Horton , John Richard Nelson , Michael John Gerdes , Suzana Kiel , Ralf Lenigk , Alexander Schenk , Wei Sun
IPC: C12N15/10 , C12Q1/68 , G01N27/447 , G01N33/53
CPC classification number: C12N15/1013 , C12N15/101 , C12Q1/6804 , C12Q1/6888 , C12Q2600/124 , G01N27/44743 , G01N27/44747 , G01N33/5308 , G01N33/689 , C12Q2522/101
Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.
Abstract translation: 本发明一般涉及从生物样品中或在生物样品中捕获精子核酸的方法和试剂盒。 在一个实施方案中，该方法包括使样品与裂解溶液接触，其具有鱼精蛋白-DNA复合物，以裂解细胞并施用鱼精蛋白特异性抗体。 这导致鱼精蛋白特异性抗体结合鱼精蛋白-DNA以形成可被捕获，纯化或检测的复合物。 还提供了用于实施所公开的方法的套件。
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公开(公告)号：US20160281153A1
公开(公告)日：2016-09-29
申请号：US15177149
申请日：2016-06-08
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson
CPC classification number: C12Q1/6853 , C12P19/34 , C12Q1/6865 , C12Q2521/301 , C12Q2523/31 , C12Q2525/179 , C12Q2531/119 , C12Q2531/125 , C12Q2531/143
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal DNA amplification methods that employ a strand displacing DNA polymerase and polyamine-oligonucleotide conjugate primer are also provided.
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