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公开(公告)号：US20210402749A1
公开(公告)日：2021-12-30
申请号：US17342422
申请日：2021-06-08
申请人： Illumina, Inc.
IPC分类号： B32B27/30 , B01J19/00 , C12Q1/6837 , C12Q1/6876 , B01L3/00 , C12Q1/6874 , C08L33/26
摘要： Embodiments of the present application relate to patterned polymer sheets and processes to prepare the same for sequencing applications. In particular, flexible micro- and nano-patterned polymer sheets are prepared and used as a template surface in sequencing reaction and new polish-free methods of forming isolated hydrogel plugs in nanowells are described.
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公开(公告)号：US20210379858A1
公开(公告)日：2021-12-09
申请号：US17409649
申请日：2021-08-23
申请人： ILLUMINA, INC.
摘要： Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
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公开(公告)号：US10280454B2
公开(公告)日：2019-05-07
申请号：US15470577
申请日：2017-03-27
申请人： Illumina, Inc.
发明人： M. Shane Bowen , Kevin L. Gunderson , Shengrong Lin , Maria Candelaria Rogert Bacigalupo , Kandaswamy Vijayan , Yir-Shyuan Wu , Bala Murali Venkatesan , James Tsay , John M. Beierle , Lorenzo Berti , Sang Ryul Park
摘要： A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.
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公开(公告)号：US20180371535A1
公开(公告)日：2018-12-27
申请号：US16118268
申请日：2018-08-30
申请人： ILLUMINA, INC.
发明人： M. Shane Bowen , Kevin L. Gunderson , Shengrong Lin , Maria Candelaria Rogert Bacigalupo , Kandaswamy Vijayan , Yir-Shyuan Wu , Bala Murali Venkatesan , James Tsay , John M. Beierle , Lorenzo Berti , Sang Ryul Park
IPC分类号： C12Q1/6844 , C12Q1/686 , B01J19/00 , C40B50/18
摘要： A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.
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公开(公告)号：US20170335380A1
公开(公告)日：2017-11-23
申请号：US15672161
申请日：2017-08-08
申请人： Illumina, Inc.
发明人： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin L. Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
CPC分类号： C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
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公开(公告)号：US09670535B2
公开(公告)日：2017-06-06
申请号：US14194143
申请日：2014-02-28
申请人： Illumina, Inc.
发明人： M. Shane Bowen , Kevin L. Gunderson , Shengrong Lin , Maria Candelaria Rogert Bacigalupo , Kandaswamy Vijayan , Yir-Shyuan Wu , Bala Murali Venkatesan , James Tsay , John M. Beierle , Lorenzo Berti , Sang Ryul Park
CPC分类号： C12Q1/6844 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00662 , B01J2219/00722 , B01J2219/00788 , C12Q1/686 , C40B50/18 , C12Q2527/125 , C12Q2535/122 , C12Q2565/543
摘要： A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.
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公开(公告)号：US08778849B2
公开(公告)日：2014-07-15
申请号：US13661524
申请日：2012-10-26
申请人： Illumina, Inc.
发明人： M. Shane Bowen , Kevin L. Gunderson , Shengrong Lin , Maria Candelaria Rogert Bacigalupo , Kandaswamy Vijayan , Yir-Shyuan Wu , Bala Murali Venkatesan , James Tsay , John M. Beierle , Lorenzo Berti , Sang Ryul Park
CPC分类号： C12Q1/6844 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00662 , B01J2219/00722 , B01J2219/00788 , C12Q1/686 , C40B50/18 , C12Q2527/125 , C12Q2535/122 , C12Q2565/543
摘要： A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.
摘要翻译： 设计的微阵列在基底上的多个位点的每一个处捕获一个或多个感兴趣的分子。 这些部位包括促进分子在部位附着的基底垫，例如聚合物基底垫。 微阵列可以通过一种或多种图案化技术制成，以产生所需图案中的基底布局。 此外，微阵列可以包括在站点鼓励克隆性的特征。
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公开(公告)号：US12017214B2
公开(公告)日：2024-06-25
申请号：US17410943
申请日：2021-08-24
申请人： Illumina, Inc.
IPC分类号： B01L3/00 , B29C65/00 , B29C65/14 , B29C65/16 , B29C65/82 , B29K507/04 , B29L9/00 , B29L31/00 , B29L31/34 , B81C1/00 , B81C3/00 , C12Q1/6874
CPC分类号： B01L3/502707 , B29C65/1435 , B29C65/1467 , B29C65/1477 , B29C65/1483 , B29C65/1635 , B29C65/1677 , B29C65/1683 , B29C66/112 , B29C66/1122 , B29C66/114 , B29C66/45 , B29C66/53461 , B29C66/71 , B29C66/72326 , B29C66/739 , B29C66/746 , B29C66/7465 , B81C1/00071 , B81C3/001 , C12Q1/6874 , B01L2200/0689 , B01L2200/12 , B01L2300/0645 , B01L2300/0816 , B01L2300/0877 , B01L2300/0887 , B01L2300/12 , B29C65/1409 , B29C65/1606 , B29C65/1609 , B29C65/1612 , B29C65/1654 , B29C65/8223 , B29C65/8246 , B29C66/026 , B29C66/028 , B29C66/72321 , B29C66/72325 , B29C66/7392 , B29C66/7394 , B29K2507/04 , B29L2009/005 , B29L2031/34 , B29L2031/756 , B81B2201/058 , B81C2201/019 , B81C2203/035 , B29C66/71 , B29K2079/08
摘要： In an example of a method of making a flowcell, an organic solid support including sidewalls and a top is provided. A bottom surface of the organic solid support adjacent to the sidewalls provides a laser bonding foot. In the method, the laser bonding foot is bonded to an inorganic solid support to form a channel having sidewalls and a top defined by the organic solid support.
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公开(公告)号：US10807089B2
公开(公告)日：2020-10-20
申请号：US15902590
申请日：2018-02-22
申请人： ILLUMINA, INC.
IPC分类号： B01L3/00 , B81C1/00 , B29C65/14 , B29C65/16 , B29C65/00 , B81C3/00 , C12Q1/6874 , B29K507/04 , B29C65/82 , B29L31/00 , B29L9/00 , B29L31/34
摘要： A fluidic device including an inorganic solid support attached to an organic solid support by a bonding layer, wherein the inorganic solid support has a rigid structure and wherein the bonding layer includes a material that absorbs radiation at a wavelength that is transmitted by the inorganic solid support or the organic solid support; and a channel formed by the inorganic solid support and the organic solid support, wherein the bonding layer that attaches the inorganic solid support to the organic solid support provides a seal against liquid flow. Methods for making fluidic devices, such as this, are also provided.
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公开(公告)号：US20200282693A1
公开(公告)日：2020-09-10
申请号：US16880809
申请日：2020-05-21
申请人： ILLUMINA, INC.
摘要： Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
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