公开(公告)号：US11952619B2

公开(公告)日：2024-04-09

申请号：US16868308

申请日：2020-05-06

申请人： ILLUMINA, INC.

发明人： Peyton Shieh ,  John M. Beierle ,  Michael S. Graige ,  Alexander Fuhrmann ,  Randall Smith ,  Wei Wei ,  Naiqian Zhan

IPC分类号： C12Q1/6837

CPC分类号： C12Q1/6837 ,  C12Q1/6837 ,  C12Q2565/507

摘要： An array includes a support including a plurality of discrete wells, a gel material positioned in each of the plurality of discrete wells, and a quality control tracer grafted to the gel material in each of the plurality of discrete wells. The quality control tracer comprises (a) a cleavable nucleotide sequence comprising a cleavage site and (b) a detectable label; and in some aspects, is a cleavable nucleotide sequence with a detectable label and a non-reactive nucleotide sequence or a primer nucleotide sequence.

公开(公告)号：US20170197193A1

公开(公告)日：2017-07-13

申请号：US15470577

申请日：2017-03-27

申请人： Illumina, Inc.

发明人： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC分类号： B01J19/00 ,  C12Q1/68 ,  C40B50/18

CPC分类号： C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

摘要： A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.

公开(公告)号：US20140200158A1

公开(公告)日：2014-07-17

申请号：US14194143

申请日：2014-02-28

申请人： Illumina, Inc.

发明人： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

摘要： A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.

摘要翻译： 设计的微阵列在基底上的多个位点的每一个处捕获一个或多个感兴趣的分子。 这些部位包括促进分子在部位附着的基底垫，例如聚合物基底垫。 微阵列可以通过一种或多种图案化技术制成，以产生所需图案中的基底布局。 此外，微阵列可以包括在站点鼓励克隆性的特征。

公开(公告)号：US20210208055A1

公开(公告)日：2021-07-08

申请号：US17191612

申请日：2021-03-03

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC分类号： G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26 ,  B01J19/00

摘要： A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US20200179935A1

公开(公告)日：2020-06-11

申请号：US16790147

申请日：2020-02-13

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Xavier von Hatten ,  Michel Perbost ,  Heng Huang ,  Nicole Lee ,  Vicky V. Lam ,  Nilda Juan ,  Timothy J. Merkel ,  John M. Beierle

IPC分类号： B01L3/00 ,  C08L83/10 ,  C08G77/44

摘要： Disclosed herein are compositions and fluidic devices that include a filler fluid having a siloxane block co-polymer solubilized in the filler fluid. Also disclosed herein are related kits and methods for using the fluidic devices for various uses, such as the polymerase chain reaction or preparations for sequencing reactions.

公开(公告)号：US20180217170A1

公开(公告)日：2018-08-02

申请号：US15936365

申请日：2018-03-26

申请人： Illumina, Inc. ,  Illumina Singapore Pte Ltd

发明人： Beng Keong Ang ,  Heng Kuang Cheng ,  John M. Beierle ,  Bradley Kent Drews ,  David Kaplan

IPC分类号： G01N35/00 ,  G01N35/10 ,  G01N35/04 ,  G01N21/64 ,  G01N35/02

摘要： Detection apparatus includes a microfluorometer having an objective, an excitation radiation source, and a detector. The detection apparatus also includes a fluidic system for delivering reagents from a reagent cartridge to a flow cell. The fluidic system includes a manifold body having a plurality of fluidic channels configured for fluid communication between the reagent cartridge and the flow cell. The fluidic system also includes a plurality of reagent sippers. The fluidic system also includes a valve configured to mediate fluid between reagent reservoirs and the flow cell. The detection apparatus also includes a flow cell latch clamp module having a clamp cover for holding the flow cell. The objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector. The microfluorometer is movable to acquire wide-field images of different areas of the flow cell.

公开(公告)号：US11060135B2

公开(公告)日：2021-07-13

申请号：US16118268

申请日：2018-08-30

申请人： ILLUMINA, INC.

发明人： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC分类号： C12Q1/6844 ,  C40B50/18 ,  C12Q1/686 ,  B01J19/00 ,  C40B40/06

摘要： A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.

公开(公告)号：US20180195950A1

公开(公告)日：2018-07-12

申请号：US15848450

申请日：2017-12-20

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC分类号： G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26

摘要： A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US20130116153A1

公开(公告)日：2013-05-09

申请号：US13661524

申请日：2012-10-26

申请人： ILLUMINA, INC.

发明人： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC分类号： B01J19/00

CPC分类号： C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

摘要： A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.

摘要翻译： 设计的微阵列在基底上的多个位点的每一个处捕获一个或多个感兴趣的分子。 这些部位包括促进分子在部位附着的基底垫，例如聚合物基底垫。 微阵列可以通过一种或多种图案化技术制成，以产生所需图案中的基底布局。 此外，微阵列可以包括在站点鼓励克隆性的特征。

公开(公告)号：US12084474B2

公开(公告)日：2024-09-10

申请号：US16411913

申请日：2019-05-14

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC分类号： C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876

CPC分类号： C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q2523/107 ,  C12Q2525/113 ,  C12Q2565/543

摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.