公开(公告)号：US20180195950A1

公开(公告)日：2018-07-12

申请号：US15848450

申请日：2017-12-20

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US11846580B2

公开(公告)日：2023-12-19

申请号：US17191612

申请日：2021-03-03

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26 ,  G01N15/10 ,  B01J19/00

CPC classification number: G01N15/1436 ,  B01J19/0046 ,  B01L3/502 ,  B01L3/502707 ,  C09D133/24 ,  C09D133/26 ,  C12Q1/6874 ,  G01N15/1484 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00418 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00722 ,  B01L2200/12 ,  B01L2300/087 ,  B01L2300/0829 ,  B01L2300/0848 ,  B01L2300/0887 ,  B01L2300/16 ,  G01N2015/1006

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US20200340046A1

公开(公告)日：2020-10-29

申请号：US16868308

申请日：2020-05-06

Applicant: ILLUMINA, INC.

Inventor： Peyton Shieh ,  John M. Beierle ,  Michael S. Graige ,  Alexander Fuhrmann ,  Randall Smith ,  Wei Wei ,  Naiqian Zhan

IPC: C12Q1/6837

Abstract: An array includes a support including a plurality of discrete wells, a gel material positioned in each of the plurality of discrete wells, and a quality control tracer grafted to the gel material in each of the plurality of discrete wells. The quality control tracer comprises (a) a cleavable nucleotide sequence comprising a cleavage site and (b) a detectable label; and in some aspects, is a cleavable nucleotide sequence with a detectable label and a non-reactive nucleotide sequence or a primer nucleotide sequence.

公开(公告)号：US20190352327A1

公开(公告)日：2019-11-21

申请号：US16411913

申请日：2019-05-14

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC: C07H21/04 ,  C12Q1/6876

Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：US11952619B2

公开(公告)日：2024-04-09

申请号：US16868308

申请日：2020-05-06

Applicant: ILLUMINA, INC.

Inventor： Peyton Shieh ,  John M. Beierle ,  Michael S. Graige ,  Alexander Fuhrmann ,  Randall Smith ,  Wei Wei ,  Naiqian Zhan

IPC: C12Q1/6837

CPC classification number: C12Q1/6837 ,  C12Q1/6837 ,  C12Q2565/507

Abstract: An array includes a support including a plurality of discrete wells, a gel material positioned in each of the plurality of discrete wells, and a quality control tracer grafted to the gel material in each of the plurality of discrete wells. The quality control tracer comprises (a) a cleavable nucleotide sequence comprising a cleavage site and (b) a detectable label; and in some aspects, is a cleavable nucleotide sequence with a detectable label and a non-reactive nucleotide sequence or a primer nucleotide sequence.

公开(公告)号：US20210208055A1

公开(公告)日：2021-07-08

申请号：US17191612

申请日：2021-03-03

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26 ,  B01J19/00

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US20240400605A1

公开(公告)日：2024-12-05

申请号：US18806031

申请日：2024-08-15

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC: C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：US12084474B2

公开(公告)日：2024-09-10

申请号：US16411913

申请日：2019-05-14

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC: C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q2523/107 ,  C12Q2525/113 ,  C12Q2565/543

Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：US20240271191A1

公开(公告)日：2024-08-15

申请号：US18611520

申请日：2024-03-20

Applicant: ILLUMINA, INC.

Inventor： Peyton Shieh ,  John M. Beierle ,  Michael S. Graige ,  Alexander Fuhrmann ,  Randall Smith ,  Wei Wei ,  Naiqian Zhan

IPC: C12Q1/6837

CPC classification number: C12Q1/6837

Abstract: An array includes a support including a plurality of discrete wells, a gel material positioned in each of the plurality of discrete wells, and a quality control tracer grafted to the gel material in each of the plurality of discrete wells. The quality control tracer comprises (a) a cleavable nucleotide sequence comprising a cleavage site and (b) a detectable label; and in some aspects, is a cleavable nucleotide sequence with a detectable label and a non-reactive nucleotide sequence or a primer nucleotide sequence.

公开(公告)号：US10955332B2

公开(公告)日：2021-03-23

申请号：US15848450

申请日：2017-12-20

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26 ,  B01J19/00 ,  G01N15/10

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.