-
公开(公告)号：US20220154273A1
公开(公告)日：2022-05-19
申请号：US17525530
申请日：2021-11-12
发明人： Gianluca Andrea Artioli , Mathieu Lessard-Viger , Brian D. Mather , Seth M. McDonald , Kaitlin M. Pugliese , Xavier von Hatten
IPC分类号： C12Q1/6874 , B01L3/00
摘要： An example of an incorporation mix includes a liquid carrier, a complex, and a labeled nucleotide. The complex includes a polymerase and a plasmonic nanostructure linked to the polymerase. The labeled nucleotide includes a nucleotide, a 3′ OH blocking group attached to a sugar of the nucleotide, and a dye label attached to a base of the nucleotide.
-
公开(公告)号：US20200325467A1
公开(公告)日：2020-10-15
申请号：US16626221
申请日：2018-06-28
发明人： Brian D. Mather , Cyril Delattre , Tarun Kumar Khurana , Yir-Shyuan Wu , Pallavi Daggumati , Behnam Javanmardi , Filiz Gorpe-Yasar , Sebastien Georg Gabriel Ricoult , Xavier von Hatten , Daniel Leonard Fuller
IPC分类号： C12N15/10 , C08F226/10
摘要： An example of a nucleic acid extraction material includes a substrate. The nucleic acid extraction material also includes a polycation bonded to at least a portion of a surface of the substrate. The polycation consists of a polymer of a quaternized monomer selected from the group consisting of a quaternized 1-vinylimidazole monomer and a quaternized dimethylaminoethyl methacrylate monomer, or a copolymer of a neutral monomer and the quaternized monomer.
-
公开(公告)号：US20180327832A1
公开(公告)日：2018-11-15
申请号：US15969562
申请日：2018-05-02
发明人： Sean M. Ramirez , Brian D. Mather , Edwin Li , Sojeong Moon , Innsu Daniel Kim , Alexandre Richez , Ludovic Vincent , Xavier von Hatten , Hai Quang Tran , Maxwell Zimmerley , Julia Morrison , Gianluca Andrea Artioli , Krystal Sly , Hayden Black , Lewis J. Kraft , Hong Xie , Wei Wei , Ryan Sanford
IPC分类号： C12Q1/6874 , C12Q1/6806
CPC分类号： C12Q1/6874 , C12Q1/6806
摘要： An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.
-
公开(公告)号：US20230416435A1
公开(公告)日：2023-12-28
申请号：US18330298
申请日：2023-06-06
发明人： Gianluca Andrea Artioli , Timothy J.N. Beech , Mathieu Lessard-Viger , Rebecca Turk-MacLeod , Brian D. Mather , Weixian Xi , Xavier von Hatten
IPC分类号： C08F220/56 , C08J3/075 , C12Q1/6869
CPC分类号： C08F220/56 , C08J3/075 , C12Q1/6869 , C08J2333/26
摘要： A co-polymer includes a plurality of a first monomer including a terminal functional group that is to attach to at least two different primers; a plurality of a second monomer including a second functional group that is different from the terminal functional group, and that is selected from the group consisting of a phenyl group, methoxy propyl, glycosyl, vinyl pyrrolidone, and an imidazole group; and a plurality of a third monomer that is different from the first and second monomers. This co-polymer may be used in a flow cell, and may enhance the clustering efficiency and kinetics.
-
公开(公告)号：US20230272469A1
公开(公告)日：2023-08-31
申请号：US18195321
申请日：2023-05-09
发明人： Sean M. Ramirez , Brian D. Mather , Edwin Li , Sojeong Moon , Innsu Daniel Kim , Alexandre Richez , Ludovic Vincent , Xavier von Hatten , Hai Quang Tran , Maxwell Zimmerley , Julia Morrison , Gianluca Andrea Artioli , Krystal Sly , Hayden Black , Lewis J. Kraft , Hong Xie , Wei Wei , Ryan Sanford
IPC分类号： C12Q1/6874 , C12Q1/6806 , B01L3/00
CPC分类号： C12Q1/6874 , C12Q1/6806 , B01L3/502707 , B01L2300/165 , B01L2300/0819 , B01L2300/0887 , B01L2300/16 , B01L2300/0877
摘要： An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.
-
公开(公告)号：US20220154177A1
公开(公告)日：2022-05-19
申请号：US17525553
申请日：2021-11-12
IPC分类号： C12N15/10 , C08F265/10 , C08J3/075
摘要： An example of a functionalized plasmonic nanostructure includes a plasmonic nanostructure core; a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm; and a plurality of primers attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core.
-
公开(公告)号：US20200179935A1
公开(公告)日：2020-06-11
申请号：US16790147
申请日：2020-02-13
发明人： Xavier von Hatten , Michel Perbost , Heng Huang , Nicole Lee , Vicky V. Lam , Nilda Juan , Timothy J. Merkel , John M. Beierle
摘要： Disclosed herein are compositions and fluidic devices that include a filler fluid having a siloxane block co-polymer solubilized in the filler fluid. Also disclosed herein are related kits and methods for using the fluidic devices for various uses, such as the polymerase chain reaction or preparations for sequencing reactions.
-
公开(公告)号：US20240117426A1
公开(公告)日：2024-04-11
申请号：US18469440
申请日：2023-09-18
发明人： Nam Nguyen , Xavier von Hatten , Will Tovey , Andrew Brown , Wayne George , Eric Brustad , Gianluca Artioli
IPC分类号： C12Q1/6874 , C08F120/06 , C08F120/56 , C08F120/60 , C08F122/38 , C08F138/02
CPC分类号： C12Q1/6874 , C08F120/06 , C08F120/56 , C08F120/60 , C08F122/385 , C08F138/02
摘要： In some examples, novel nanogel particles are described having dual functionality, temperature responsiveness and pH responsiveness. For nucleic acid sequencing, amplification primers are grafted to nanogel particles to form primer-grafted nanogel particles, and the primer-grafted nanogel particles are captured onto surfaces within a flow cell. Within flow cells such as used in SBS nucleic acid sequencing, each primer-grafted nanogel particle functions as a nano-well in the flow cell, thus eliminating the need for nano-wells in some examples.
-
公开(公告)号：US20240150508A1
公开(公告)日：2024-05-09
申请号：US18482417
申请日：2023-10-06
发明人： Nam Nguyen , Xavier von Hatten , Wayne George , Gianluca Artioli , Brian Mather , Johan Basuki , Shima Gholizadeh
IPC分类号： C08F220/56 , C08J3/24 , C08J3/28
CPC分类号： C08F220/56 , C08J3/245 , C08J3/28 , C08F2810/20 , C08F2810/40 , C08J2333/26
摘要： In some examples, novel photochemically-reversible hydrogels and nanogel particles are described comprising copolymer chains including at least one reactive alkene or reactive 1,4-diene end group capable of [2+2] or [2+2+2+2] photodimerization, respectively, at wavelengths >270 nm. In various examples, the photochemically-reversible hydrogels comprise copolymer chains including at least one —N3, —C≡CH or —CO2H end group for dual functionality and/or pH responsiveness. For nucleic acid sequencing, amplification primers are grafted to photochemically-reversible hydrogels or nanogel particles reversibly bound to surfaces within a flow cell. After sequencing is complete, the photochemically-reversible hydrogel or nanogel particles is/are removable from the flow cell surfaces by irradiation, enabling the flow cell to be reusable.
-
公开(公告)号：US20190217300A1
公开(公告)日：2019-07-18
申请号：US15770017
申请日：2016-10-20
发明人： Xavier von Hatten , Michel Perbost , Heng Huang , Nicole Lee , Vicky V. Lam , Nilda Juan , Timothy J. Merkel , John M. Beierle
CPC分类号： B01L3/502792 , B01J19/0093 , B01J2219/00813 , B01L2300/12 , B01L2400/0418 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , C08G77/04 , C08G77/388 , C08G77/392 , C08G77/44 , C08L83/10 , C08L83/00
摘要： Disclosed herein are compositions and fluidic devices that include a filler fluid having a siloxane block co-polymer solubilized in the filler fluid. Also disclosed herein are related kits and methods for using the fluidic devices for various uses, such as the polymerase chain reaction or preparations for sequencing reactions.
-
-
-
-
-
-
-
-
-
搜索结果
31 条记录 (耗时 0.023 秒)
