公开(公告)号：US11692941B2

公开(公告)日：2023-07-04

申请号：US17509806

申请日：2021-10-25

Applicant: Optofluidic Bioassay, LLC

Inventor： Maung Kyaw Khaing Oo ,  Xudong Fan

IPC: G01N21/77 ,  B01L3/00 ,  G01N21/78 ,  G01N21/76 ,  G01N21/75

CPC classification number: G01N21/77 ,  B01L3/5085 ,  G01N21/78 ,  B01L2300/0663 ,  B01L2300/0809 ,  B01L2300/0829 ,  B01L2300/0832 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/168 ,  G01N21/76 ,  G01N2021/757 ,  G01N2021/7786 ,  G01N2201/0446

Abstract: An optofluidic diagnostic system and methods for rapid analyte detections. The system comprises an optofluidic sensor array, a test plate and an optical detection cartridge. The sensor array supports one or more distinct sensor units, each having a reactor section designed to temporarily enter a series of different kinds of wells in the test plate. One kind of well is a sample reservoir that holds reagent solution to be transferred into the reactor section. Another kind of well is a drainage chamber that removes reagent solution from the reactor section. A third kind of well is a colorant reservoir that holds a colorant reagent transferable into a reactor section. Finally, the sensor unit is transferred to the optical detection cartridge where it is placed into an isolation booth during the optical detection process so that its flat observation face is stationed in a viewing window opposite an optical detector lens.

公开(公告)号：US20190064168A1

公开(公告)日：2019-02-28

申请号：US16115059

申请日：2018-08-28

Applicant: Celsee Diagnostics, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: G01N33/574 ,  B01L3/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/527 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/0406 ,  C12M47/04 ,  C12Q1/6816 ,  C12Q1/6886 ,  G01N15/1434 ,  G01N15/1484 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  G01N2015/1006 ,  G01N2015/149

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: distributing a population of cells and a population of non-cell particles across the array of wells through the fluid reservoir to increase capture efficiency of individual cell-particle pairs within the array of wells, and processing the captured cell-particle pairs at the set of wells.

公开(公告)号：US20180353964A1

公开(公告)日：2018-12-13

申请号：US16107826

申请日：2018-08-21

Applicant: Abbott Laboratories

Inventor： Robert Paul Luoma, II ,  Brian Ochranek ,  Cheryl L. Davidson ,  Bradley C. Weston

IPC: B01L3/00 ,  G01N35/10

CPC classification number: B01L3/52 ,  B01F9/002 ,  B01F9/003 ,  B01F11/0002 ,  B01F11/0005 ,  B01F11/0008 ,  B01F2009/0092 ,  B01L3/5082 ,  B01L2200/0684 ,  B01L2300/0851 ,  B01L2400/086 ,  G01N35/1002 ,  G01N2035/00524 ,  G01N2035/0439 ,  G01N2035/1025

Abstract: Methods and apparatus to mitigate bubble formation in a liquid are disclosed. An example apparatus disclosed herein includes a bottom wall, a first baffle cantilevered from the bottom wall, and a second baffle cantilevered from the bottom wall. The first baffle is spaced apart from the second baffle, and the first baffle and the second baffle are positioned radially relative to an axis of rotation of the apparatus.

公开(公告)号：US20180318835A1

公开(公告)日：2018-11-08

申请号：US16035464

申请日：2018-07-13

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Rhett Martineau ,  Jeff Houkal ,  Shih-Hui Chao ,  Weimin Gao ,  Shufang Ci ,  Deirdre Meldrum

IPC: B01L3/00 ,  B01L7/00

CPC classification number: B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0803 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/161 ,  B01L2400/049 ,  B01L2400/0683

Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.

公开(公告)号：US20180304254A1

公开(公告)日：2018-10-25

申请号：US16026827

申请日：2018-07-03

Applicant: Combinati Incorporated

Inventor： Ju-Sung Hung ,  Andrew Zayac ,  Megan Dueck

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/686

CPC classification number: B01L3/50273 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2300/0609 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/18 ,  B01L2400/0487 ,  B01L2400/049 ,  C12Q1/686 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.

公开(公告)号：US20180273583A1

公开(公告)日：2018-09-27

申请号：US15927934

申请日：2018-03-21

Applicant: Unchained Labs

Inventor： David Wesley WYRICK ,  Stuart Kazumi MORITA ,  Lynn COMISKEY ,  David Eugene EBERHART

IPC: C07K1/34 ,  C07K16/00 ,  G01N1/34 ,  B01D61/14 ,  B01D61/02

CPC classification number: C07K1/34 ,  B01D61/027 ,  B01D61/145 ,  B01L3/50255 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0851 ,  C07K16/00 ,  G01N1/34 ,  G01N2001/4088

Abstract: Sample plates and methods for exchanging buffer solutions are disclosed herein. The sample plates and methods may be used with automated buffer exchange systems where high pressures, for example, pressures of at least about 30 psig, are applied across a filtering membrane. Methods for manufacturing the sample plates are further disclosed.

公开(公告)号：US20180264465A1

公开(公告)日：2018-09-20

申请号：US15539045

申请日：2014-12-22

Applicant: Ecole Polytechnique Federale De Lausanne

Inventor： Sylke Höhnel ,  Nathalie Brandenberg ,  Matthias Lutolf

IPC: B01L3/00 ,  C12M1/32 ,  C12M3/06

CPC classification number: B01L3/5085 ,  B01L2200/026 ,  B01L2200/0668 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0893 ,  B01L2300/165 ,  C12M1/32 ,  C12M3/06 ,  C12M23/12 ,  C12M23/16 ,  C12M23/20

Abstract: The invention concerns a device (1) for aggregating cells comprising at least one cavity (2), wherein said cavity comprises a plurality of microwells (3) for receiving at least one cell, wherein each said well comprises a vertical sidewall and a curved bottom.

公开(公告)号：US10078778B2

公开(公告)日：2018-09-18

申请号：US14997439

申请日：2016-01-15

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G01N15/14 ,  G01N35/00 ,  G02B21/16 ,  H04N5/225 ,  G02B21/26 ,  B01L3/00 ,  G06K9/00 ,  G01N33/483 ,  G06K9/03 ,  G06K9/46 ,  G01N15/00 ,  G01N15/10

CPC classification number: G06K9/00147 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0896 ,  B01L2300/12 ,  G01N15/1425 ,  G01N15/1463 ,  G01N33/4833 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2035/00148 ,  G02B21/16 ,  G02B21/26 ,  G06K9/0014 ,  G06K9/036 ,  G06K9/4647 ,  G06K9/4652 ,  G06K9/4661 ,  H04N5/2256

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

公开(公告)号：US20180257075A1

公开(公告)日：2018-09-13

申请号：US15750418

申请日：2016-08-10

Applicant: Duke University

Inventor： Benjamin B. Yellen ,  David M. Murdoch ,  Roozbeh Abedini-Nassab ,  Kris C. Wood

IPC: B01L3/00 ,  B03C1/28

CPC classification number: B01L3/502761 ,  B01L2200/025 ,  B01L2200/0668 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/0893 ,  B01L2400/043 ,  B01L2400/0605 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/0666 ,  B01L2400/0677 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26

Abstract: The current invention relates to systems for transporting, trapping, and releasing magnetically-labeled bioparticles within microfluidic environments.

公开(公告)号：US20180257068A1

公开(公告)日：2018-09-13

申请号：US15918621

申请日：2018-03-12

Applicant: William Brinton

Inventor： William Brinton

IPC: B01L3/00 ,  G01N33/00 ,  G01N21/75

CPC classification number: B01L3/50 ,  B01L3/508 ,  B01L2200/18 ,  B01L2300/027 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0627 ,  B01L2300/0832 ,  B01L2300/0851 ,  G01N21/3504 ,  G01N21/75 ,  G01N33/0004 ,  G01N2021/8466

Abstract: A biological sample analysis device that is an easily portable free-standing device for determining the rate of deterioration of biological samples.