公开(公告)号：US20240335835A1

公开(公告)日：2024-10-10

申请号：US18609494

申请日：2024-03-19

Applicant: Combinati Incorporated

Inventor： Ju-Sung HUNG ,  Andrew ZAYAC ,  Megan DUECK

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/686

CPC classification number: B01L3/50273 ,  B01L7/52 ,  C12Q1/686 ,  B01L2200/0605 ,  B01L2300/0609 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/18 ,  B01L2400/0487 ,  B01L2400/049

Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.

公开(公告)号：US12091711B2

公开(公告)日：2024-09-17

申请号：US17578942

申请日：2022-01-19

Applicant: Combinati Incorporated

Inventor： Ju-Sung Hung ,  Megan Dueck ,  Andrew Zayac

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/686

CPC classification number: C12Q1/686 ,  B01L3/502715 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2400/0487 ,  B01L2400/049 ,  C12Q1/686 ,  C12Q2527/107 ,  C12Q2565/629

Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.

公开(公告)号：US20230347353A1

公开(公告)日：2023-11-02

申请号：US18002310

申请日：2021-06-21

Applicant: COMBINATI INCORPORATED

Inventor： Ju-Sung HUNG ,  Lingxia JIANG ,  Robert LIN

IPC: B01L7/00 ,  C12Q1/6853

CPC classification number: B01L7/525 ,  C12Q1/6853 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/1822 ,  B01L2300/0893 ,  B01L2300/0663

Abstract: The present disclosure provides methods and systems for nucleic acid identification. Identification of a nucleic acid molecule may include generating, in a plurality of chambers, a plurality of double-stranded nucleic acid molecules, denaturing the double-stranded nucleic acid molecules, and detecting signals of the denaturation to generate one or more denaturation profiles. The one or more denaturation profiles may be usable to identify nucleic acid molecules. The methods and system described herein may provide for identification of multiple nucleic acid molecules from a single analysis.

公开(公告)号：US20230035727A1

公开(公告)日：2023-02-02

申请号：US17759070

申请日：2021-01-22

Applicant: COMBINATI INCORPORATED

Inventor： Ju-Sung HUNG ,  Felicia LINN ,  Robert LIN

IPC: B01L3/00 ,  B29C71/00 ,  B29C71/02

Abstract: The present disclose provides methods for forming a microfluidic device. Methods for forming a microfluidic device may comprise providing a microfluidic structure and a film, treating a surface of the microfluidic structure, a surface of the film, or both with a solvent, subsequently pressing the microfluidic structure together with the film under a first heating condition to form the microfluidic device comprising the solvent, and applying a negative pressure to the microfluidic device under a second heating condition, which negative pressure is applied for a time period greater than 30 minutes or at a pressure less than 20 kilopascals (kPa) to remove at least a portion of the solvent. In some aspects, the present disclosure provides devices consistent with the methods herein.

公开(公告)号：US20220243254A1

公开(公告)日：2022-08-04

申请号：US17578942

申请日：2022-01-19

Applicant: Combinati Incorporated

Inventor： Ju-Sung Hung ,  Megan Dueck ,  Andrew Zayac

IPC: C12Q1/686 ,  B01L3/00 ,  B01L7/00

Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.

公开(公告)号：US20200001291A1

公开(公告)日：2020-01-02

申请号：US16270457

申请日：2019-02-07

Applicant: Combinati Incorporated

Inventor： Ju-Sung Hung ,  Andrew Zayac ,  Megan Dueck

IPC: B01L3/00 ,  C12Q1/686 ,  B01L7/00

Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.

公开(公告)号：US11567007B2

公开(公告)日：2023-01-31

申请号：US16651312

申请日：2019-07-19

Applicant: Advanced Instrument Pte. Ltd. ,  Combinati Incorporated

Inventor： Ronian Siew ,  Sheau Yeng Wei ,  Ju-Sung Hung

IPC: G01N21/64 ,  G01N21/03

Abstract: Various embodiments may provide a method of illuminating a sample plane. The method may include providing an illumination subsystem, the illumination subsystem including an optical source and at least one lens, having an optic axis at an incident angle greater than 0° and less than 90° to a normal of the sample plane. The method may also include rotating the illumination subsystem about a pivot point between the optical source and the sample plane along the optic axis so that an adjusted illumination distribution generated by the illumination subsystem at the sample plane has greater symmetry compared to a reference illumination distribution generated by the illumination subsystem at the sample plane without the rotation about the pivot point.

公开(公告)号：US20210140885A1

公开(公告)日：2021-05-13

申请号：US16651312

申请日：2019-07-19

Applicant: Advanced Instrument Pte. Ltd. ,  Combinati Incorporated

Inventor： Ronian Siew ,  Sheau Yeng Wei ,  Ju-Sung Hung

IPC: G01N21/64

Abstract: Various embodiments may provide a method of illuminating a sample plane. The method may include providing an illumination subsystem, the illumination subsystem including an optical source and at least one lens, having an optic axis at an incident angle greater than 0° and less than 90° to a normal of the sample plane. The method may also include rotating the illumination subsystem about a pivot point between the optical source and the sample plane along the optic axis so that an adjusted illumination distribution generated by the illumination subsystem at the sample plane has greater symmetry compared to a reference illumination distribution generated by the illumination subsystem at the sample plane without the rotation about the pivot point.

公开(公告)号：US20200248232A1

公开(公告)日：2020-08-06

申请号：US16669205

申请日：2019-10-30

Applicant: Combinati Incorporated

Inventor： Ju-Sung Hung ,  Megan Dueck ,  Andrew Zayac

IPC: C12Q1/686 ,  B01L3/00 ,  B01L7/00

Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.

公开(公告)号：US20190264260A1

公开(公告)日：2019-08-29

申请号：US16408041

申请日：2019-05-09

Applicant: Combinati Incorporated

Inventor： Andrew Zayac ,  Ju-Sung Hung ,  Howard Gregg King ,  David Cox

IPC: C12Q1/686 ,  B01L3/00

Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.