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公开(公告)号：US12091711B2
公开(公告)日：2024-09-17
申请号：US17578942
申请日：2022-01-19
Applicant: Combinati Incorporated
Inventor： Ju-Sung Hung , Megan Dueck , Andrew Zayac
CPC classification number: C12Q1/686 , B01L3/502715 , B01L3/502738 , B01L7/52 , B01L2200/0684 , B01L2200/16 , B01L2300/0816 , B01L2300/0864 , B01L2300/0883 , B01L2400/0487 , B01L2400/049 , C12Q1/686 , C12Q2527/107 , C12Q2565/629
Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.
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公开(公告)号：US20220243254A1
公开(公告)日：2022-08-04
申请号：US17578942
申请日：2022-01-19
Applicant: Combinati Incorporated
Inventor： Ju-Sung Hung , Megan Dueck , Andrew Zayac
Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.
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公开(公告)号：US20200001291A1
公开(公告)日：2020-01-02
申请号：US16270457
申请日：2019-02-07
Applicant: Combinati Incorporated
Inventor： Ju-Sung Hung , Andrew Zayac , Megan Dueck
Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
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公开(公告)号：US11567007B2
公开(公告)日：2023-01-31
申请号：US16651312
申请日：2019-07-19
Applicant: Advanced Instrument Pte. Ltd. , Combinati Incorporated
Inventor： Ronian Siew , Sheau Yeng Wei , Ju-Sung Hung
Abstract: Various embodiments may provide a method of illuminating a sample plane. The method may include providing an illumination subsystem, the illumination subsystem including an optical source and at least one lens, having an optic axis at an incident angle greater than 0° and less than 90° to a normal of the sample plane. The method may also include rotating the illumination subsystem about a pivot point between the optical source and the sample plane along the optic axis so that an adjusted illumination distribution generated by the illumination subsystem at the sample plane has greater symmetry compared to a reference illumination distribution generated by the illumination subsystem at the sample plane without the rotation about the pivot point.
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公开(公告)号：US20210140885A1
公开(公告)日：2021-05-13
申请号：US16651312
申请日：2019-07-19
Applicant: Advanced Instrument Pte. Ltd. , Combinati Incorporated
Inventor： Ronian Siew , Sheau Yeng Wei , Ju-Sung Hung
IPC: G01N21/64
Abstract: Various embodiments may provide a method of illuminating a sample plane. The method may include providing an illumination subsystem, the illumination subsystem including an optical source and at least one lens, having an optic axis at an incident angle greater than 0° and less than 90° to a normal of the sample plane. The method may also include rotating the illumination subsystem about a pivot point between the optical source and the sample plane along the optic axis so that an adjusted illumination distribution generated by the illumination subsystem at the sample plane has greater symmetry compared to a reference illumination distribution generated by the illumination subsystem at the sample plane without the rotation about the pivot point.
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Patent Agency Ranking
公开(公告)号：US20200248232A1
公开(公告)日：2020-08-06
申请号：US16669205
申请日：2019-10-30
Applicant: Combinati Incorporated
Inventor： Ju-Sung Hung , Megan Dueck , Andrew Zayac
Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.
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公开(公告)号：US20190264260A1
公开(公告)日：2019-08-29
申请号：US16408041
申请日：2019-05-09
Applicant: Combinati Incorporated
Inventor： Andrew Zayac , Ju-Sung Hung , Howard Gregg King , David Cox
Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.
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公开(公告)号：US09845499B2
公开(公告)日：2017-12-19
申请号：US15363896
申请日：2016-11-29
Applicant: Combinati Incorporated
Inventor： Ju-Sung Hung , Andrew Zayac , Megan Dueck
CPC classification number: C12Q1/686 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L7/52 , B01L2200/0605 , B01L2200/12 , B01L2300/041 , B01L2300/0832 , B01L2300/0851 , B01L2300/0864 , B01L2300/12 , B01L2400/0487 , C12Q2563/159 , C12Q2565/629
Abstract: A microfluidic device can have a plurality of microchambers connected to a microchannel via siphon apertures. The microfluidic device may be formed from a thermoplastic and capped by a thermoplastic thin film. The microfluidic device may be used for digital polymerase chain reactions by forcing reagent into the microchambers at low pressure via an inlet, forcing any gas trapped in the microfluidic device to outgas through the thin film by applying high pressure to the microfluidic device via inlets and outlets of the microfluidic device, and applying air at low pressure to the inlets in order to digitize the chambers such that the reagent in each chamber is isolated from the reagent in other chambers by an air gap. After digitization, the device may be used to run a digital polymerase chain reaction process.
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公开(公告)号：US11697844B2
公开(公告)日：2023-07-11
申请号：US16669205
申请日：2019-10-30
Applicant: Combinati Incorporated
Inventor： Ju-Sung Hung , Megan Dueck , Andrew Zayac
CPC classification number: C12Q1/686 , B01L3/502715 , B01L3/502738 , B01L7/52 , B01L2200/0684 , B01L2200/16 , B01L2300/0816 , B01L2300/0864 , B01L2300/0883 , B01L2400/049 , B01L2400/0487 , C12Q1/686 , C12Q2527/107 , C12Q2565/629
Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.
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公开(公告)号：US11285478B2
公开(公告)日：2022-03-29
申请号：US15783743
申请日：2017-10-13
Applicant: Combinati Incorporated
Inventor： Ju-Sung Hung , Andrew Zayac , Megan Dueck
Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
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