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公开(公告)号：US10136802B2
公开(公告)日：2018-11-27
申请号：US14828873
申请日：2015-08-18
Applicant: OLYMPUS CORPORATION
Inventor： Yohei Tanikawa , Yasunobu Iga , Shinichi Takimoto
Abstract: A device for sustained release of optical clearing agent includes a stick-shaped component having at least two supplying passages for supplying gas or liquid formed on the inside with apertures of the supplying passages being formed at positions along the circumference of the lateral surface in the vicinity of the top end of the stick-shaped component respectively, at least two pouch-shaped elastic components placed at positions along the circumference of the lateral surface in the vicinity of the top end of the stick-shaped component respectively to cover the apertures of the supplying passages respectively, an optical clearing agent holding component fixed on the surface of one of the pouch-shaped elastic components and holding optical clearing agent, and a body-fluid absorbing component fixed on the surface of another one of the pouch-shaped elastic components and absorbing body fluid flowing from a target object or dehydrating the target object by osmotic pressure.
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公开(公告)号：US11226476B2
公开(公告)日：2022-01-18
申请号：US16366682
申请日：2019-03-27
Applicant: OLYMPUS CORPORATION
Inventor： Tsuyoshi Mochizuki , Shintaro Takahashi , Akira Matsushita , Yohei Tanikawa , Masaru Mizunaka , Shinichi Takimoto
IPC: G02B21/34 , G02B21/08 , G02B21/26 , G02B21/36 , G01N21/59 , G01N21/01 , G02B21/33 , G01N21/17 , C12M1/34
Abstract: An observation apparatus including: a top plate on which a container in which a specimen is accommodated can be placed, and through which illumination light can pass; a light source that emits the illumination light upward from below the specimen; an objective lens that focuses, below the specimen and the top plate, transmitted light which is the illumination light that has passed through the specimen from thereabove and that has passed through the top plate; and a camera that captures the transmitted light, wherein the light source emits the illumination light toward an area above the specimen from outside the objective lens in a radial direction, and the top plate is provided with a mark that specifies a viewing-field area of the camera.
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公开(公告)号：US11137588B2
公开(公告)日：2021-10-05
申请号：US16364946
申请日：2019-03-26
Applicant: OLYMPUS CORPORATION
Inventor： Masaru Mizunaka , Shintaro Takahashi , Akira Matsushita , Yohei Tanikawa , Tsuyoshi Mochizuki , Shinichi Takimoto
IPC: G02B21/06 , G02B21/16 , G02B21/02 , G02B21/12 , G01N21/64 , G02B21/36 , G02B21/10 , G01N21/17 , G02B21/24 , G02B21/33
Abstract: An observation apparatus including: a light-source that emits illumination light and excitation light upward from below a specimen; and an image-capturing optical system having an objective lens that focuses, below the specimen, transmitted light, which is the illumination light that is emitted from the light-source, that is reflected above the specimen, and that has passed through the specimen, and fluorescence that is generated in the specimen that has been irradiated with the excitation light emitted from the light-source, wherein the light-source is disposed radially outside the objective lens.
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公开(公告)号：US09383564B2
公开(公告)日：2016-07-05
申请号：US14495241
申请日：2014-09-24
Applicant: OLYMPUS CORPORATION
Inventor： Katsumasa Fujita , Takeharu Nagai , Kenta Saito , Masahito Yamanaka , Shinichi Takimoto
CPC classification number: G02B21/0076 , A61B6/037 , A61B6/0478 , A61B6/501 , G01N21/6458 , G01N2021/6421 , G01N2021/6471 , G01N2201/068 , G01N2201/0697 , G01T1/2985
Abstract: A fluorescence observation method of the present invention for detecting plural types of fluorescence emitted from two or more kinds of fluorescent molecules includes: subjecting each of the two or more kinds of fluorescent molecules to multi-photon excitation by exciting light having an exciting wavelength equal to or shorter than 700 nm in a visible region, to generate fluorescence upon making use of an absorption wavelength band in a deep ultraviolet region of each of the two or more kinds of fluorescent molecules; and simultaneously detecting plural types of fluorescence generated on a shorter-wavelength side or on both of the shorter-wavelength side and a longer-wavelength side of the exciting wavelength of the exciting light.
Abstract translation: 本发明的用于检测从两种以上荧光分子发出的多种荧光的荧光观察方法包括：通过激发具有激发波长的光，使两种或更多种荧光分子中的每一种进行多光子激发 或者在可见光区域中短于700nm，在使用两种或更多种荧光分子的深紫外区域中的吸收波长带产生荧光; 并且同时检测在激发光的激发波长的短波长侧或短波长侧和长波长侧的两端产生的多种荧光。
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公开(公告)号：US10317336B2
公开(公告)日：2019-06-11
申请号：US15227086
申请日：2016-08-03
Applicant: OLYMPUS CORPORATION
Inventor： Akira Sato , Shinichi Takimoto
Abstract: A spectral measurement method of the present invention includes: a measuring step of measuring optical spectra of some sections specified among a plurality of sections on a specimen; a scalar-value calculating step of calculating, for individual measured sections, scalar values that represent information contained in the obtained optical spectra; an interpolating step of interpolating scalar values of unmeasured sections by using the calculated scalar values and by using two types of interpolation methods; an identifying step of identifying sections in which absolute values of differences between the two scalar values interpolated by using the two types of interpolation methods are equal to or greater than a predetermined threshold; and a repeating step of re-executing steps from the measuring step to the identifying step after specifying the identified sections.
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Patent Agency Ranking
公开(公告)号：US10209502B2
公开(公告)日：2019-02-19
申请号：US15275359
申请日：2016-09-24
Applicant: OLYMPUS CORPORATION
Inventor： Kentaro Imoto , Shinichi Takimoto , Shintaro Takahashi , Atsushi Doi
Abstract: Provided is a microscope provided with: a scanner that scans an excitation beam coming from a light source; an objective optical system that focuses the scanned excitation beam onto a sample and that collects fluorescence generated at individual scanning positions in the sample; a detector that detects the collected fluorescence; a light blocking member that is disposed between the detector and the system and that partially blocks the collected fluorescence; a switching portion that switches the positional relationship between the member and a light-focusing point of the excitation beam in the sample between an optically conjugate positional relationship, in which an in-focus fluorescence generated at the light-focusing point passes through the member, and a non-conjugate positional relationship, in which the in-focus fluorescence is blocked by the member; and a computing portion that computes a difference between fluorescence signals acquired by the detector in the two positional relationships.
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公开(公告)号：US20180179487A1
公开(公告)日：2018-06-28
申请号：US15848623
申请日：2017-12-20
Applicant: OLYMPUS CORPORATION
Inventor： Akira MATSUSHITA , Shintaro Takahashi , Masaru Mizunaka , Shinichi Takimoto , Tadashi Hirata , Yohei Tanikawa
CPC classification number: C12M41/46 , G01J5/0003 , G01J5/04 , G01J5/061 , G01J5/0896 , G01J5/10 , G01J2005/0077 , G01J2005/068 , H04N5/2252 , H04N5/2256 , H04N5/33
Abstract: Provided is an observation apparatus including: a housing that has, in a top face thereof, a transmission window on which a container accommodating cells can be placed and through which light can pass; a camera portion that is accommodated in the housing and that captures observation light coming from the cells irradiated with illumination light emitted from an illumination portion and entering the housing by passing through a bottom face of the container and the transmission window of the housing; and a housing-interior heating portion that is accommodated in the housing and that heats the cells.
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公开(公告)号：US11348350B2
公开(公告)日：2022-05-31
申请号：US16785714
申请日：2020-02-10
Applicant: OLYMPUS CORPORATION
Inventor： Shinichi Takimoto , Yohei Tanikawa , Taiji Mine , Takayuki Nakatomi , Masakazu Fujii
Abstract: An observation system includes a processor. The processor manages a plurality of images acquired in time series with respect to a biological sample which is being cultured, records a first comment and a first region of interest (RoI) in a storage device in association with the images based on user's instructions, and records a second comment and a second ROI in the storage device in association with the first comment based on user's instructions.
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公开(公告)号：US10281704B2
公开(公告)日：2019-05-07
申请号：US15607666
申请日：2017-05-29
Applicant: OLYMPUS CORPORATION
Inventor： Shintaro Takahashi , Tadashi Hirata , Yasunobu Iga , Shinichi Takimoto , Takashi Miyoshi
Abstract: Provided is an observation apparatus and an observation method with which it is possible to observe imaging subjects, such as cells or the like, without labeling the imaging subjects and without causing an increase in the apparatus size. Provided is an observation apparatus including: a light-source unit that emits illumination light upward from below a sample; and an image-capturing optical system that has an objective lens that collects transmitted light, which is the illumination light emitted from the light-source unit that has passed through the sample by being reflected above the sample and that captures, below the sample, the transmitted light collected by the objective lens.
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公开(公告)号：US10048210B2
公开(公告)日：2018-08-14
申请号：US15233230
申请日：2016-08-10
Applicant: OLYMPUS CORPORATION
Inventor： Akira Sato , Shinichi Takimoto
Abstract: With a spectroscopic analysis method of the present invention, by performing principal component analysis on optical spectra measured at individual positions of a specimen, principal components of a plurality of orders that constitute the individual optical spectra are calculated; for the individual orders, principal-component images in which values thereof are principal-component scores of the individual principal components corresponding to the positions are created; distribution patterns of the principal-component scores are identified in the individual principal-component images; the morphology of the specimen is identified in a morphological image in which the specimen is captured; the principal-component images that have principal-component-score distribution patterns correlated with the morphology of the specimen are identified; and the individual optical spectra are reconstructed by using the principal components of orders corresponding to the orders of the identified principal-component images.
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