公开(公告)号：US11237047B2

公开(公告)日：2022-02-01

申请号：US16819406

申请日：2020-03-16

Applicant: OLYMPUS CORPORATION

Inventor： Kentaro Imoto ,  Makoto Ishikake

IPC: G01J1/44 ,  G01T1/24 ,  G01J1/04 ,  G01N21/27 ,  G01T1/29

Abstract: An optical measurement device includes: an optical sensor that detects pulsed signal light and that outputs a detection signal formed of an exponential-function response; an A/D converter that samples the detection signal output from the optical sensor and that converts the detection signal into a digital signal; and a processor comprising hardware, the processor being configured to subject the digital signal output from the A/D converter to inverse transformation by using a multiple diagonal matrix, thus calculating an estimated pulse of the signal light.

公开(公告)号：US10281699B2

公开(公告)日：2019-05-07

申请号：US15683980

申请日：2017-08-23

Applicant: OLYMPUS CORPORATION

Inventor： Tatsuo Nakata ,  Atsushi Doi ,  Kentaro Imoto

IPC: G02B21/00 ,  G02B21/06 ,  G02B21/24

Abstract: A microscope system includes an objective lens that focuses, on a specimen, illumination light produced by a light source. An illumination-area switching mechanism is disposed between the objective lens and the specimen and switches an illumination area irradiated with the illumination light focused by the objective lens among a plurality of illumination areas on the specimen that are located outside an objective optical axis of the objective lens. An inner focus lens is disposed on the objective optical axis between the light source and the objective lens and changes a focus position of the objective lens in a direction along the objective optical axis.

公开(公告)号：US10261299B2

公开(公告)日：2019-04-16

申请号：US16102840

申请日：2018-08-14

Applicant: OLYMPUS CORPORATION

Inventor： Kentaro Imoto ,  Ryusuke Tanaka ,  Hidenori Tsuboi

IPC: G01N21/64 ,  G02B21/00

Abstract: Provided is an image-acquisition apparatus including: a scanning portion that scans illumination light emitted from a light source; an optical system that focuses the scanned illumination light on a sample, while collecting signal light beams generated at the individual scanning positions on the sample; a detector that detects the collected signal light beams and that generates detection signals thereof; a signal controller that is configured to generate periodic signals that are repeated at a predetermined period; a light controller that is configured to temporally control a position or an intensity of the illumination light in accordance with the generated periodic signals; and a computer that is configured to process, in accordance with the generated periodic signals, the detection signals generated, wherein the computer is provided with a frequency shifter for shifting frequencies of the detection signals, and an integrator that integrates the shifted detection signals at an integration time.

公开(公告)号：US10209502B2

公开(公告)日：2019-02-19

申请号：US15275359

申请日：2016-09-24

Applicant: OLYMPUS CORPORATION

Inventor： Kentaro Imoto ,  Shinichi Takimoto ,  Shintaro Takahashi ,  Atsushi Doi

IPC: G02B21/00 ,  G01N21/64

Abstract: Provided is a microscope provided with: a scanner that scans an excitation beam coming from a light source; an objective optical system that focuses the scanned excitation beam onto a sample and that collects fluorescence generated at individual scanning positions in the sample; a detector that detects the collected fluorescence; a light blocking member that is disposed between the detector and the system and that partially blocks the collected fluorescence; a switching portion that switches the positional relationship between the member and a light-focusing point of the excitation beam in the sample between an optically conjugate positional relationship, in which an in-focus fluorescence generated at the light-focusing point passes through the member, and a non-conjugate positional relationship, in which the in-focus fluorescence is blocked by the member; and a computing portion that computes a difference between fluorescence signals acquired by the detector in the two positional relationships.

公开(公告)号：US11307140B2

公开(公告)日：2022-04-19

申请号：US16715088

申请日：2019-12-16

Applicant: OLYMPUS CORPORATION

Inventor： Kentaro Imoto

IPC: G01N21/64

Abstract: A signal acquisition device includes: a light source that oscillates pulsed laser light at a specific repetition period; an optical system that focuses the laser light onto a sample, and that collects generated fluorescence; a photodetector that detects the fluorescence collected by the optical system; an A/D converter that samples an intensity signal of the detected fluorescence, in synchronization with the repetition period of the light source unit, at a period that is an integer multiple of the repetition period, and that generates a digital intensity signal; and one or more processors comprising hardware, the one or more processors being configured to: obtain a fluorescence lifetime waveform on a basis of the generated digital intensity signal; and calculate a fluorescence lifetime coefficient from a waveform obtained by removing a region not corresponding to an exponential function from the obtained fluorescence lifetime waveform.

公开(公告)号：US10401293B2

公开(公告)日：2019-09-03

申请号：US15909203

申请日：2018-03-01

Applicant: OLYMPUS CORPORATION

Inventor： Kentaro Imoto

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/08 ,  G02B21/16

Abstract: Provided is a microscope including: a scanner for scanning excitation light; an objective optical system that focuses the excitation light onto a sample and that collects fluorescence generated by the sample; a light-blocking member for transmitting a portion of the collected fluorescence; a detector for detecting the fluorescence; a setting unit that allows types of fluorescence having passed through the light-blocking member to be detected by the detector at different times in two types of positional relationships in which the positional relationship between the position of an opening in the light-blocking member and a focal point of the objective optical system in the sample is set to an optically conjugate positional relationship, in which in-focus fluorescence passes through the light-blocking member, and to an optically nonconjugate positional relationship; and an arithmetic operation unit for calculating the difference between fluorescence signals acquired at different times by the detector.

公开(公告)号：US10352861B2

公开(公告)日：2019-07-16

申请号：US16215809

申请日：2018-12-11

Applicant: OLYMPUS CORPORATION

Inventor： Asuka Suekane ,  Shintaro Takahashi ,  Kentaro Imoto ,  Atsushi Doi ,  Shinichi Takimoto

IPC: G01N21/64

Abstract: An observation assistance device of the present invention includes: an information storage unit that stores sample state information showing a sample state change, a type of sample, a type of fluorescent substance, and an observation condition in fluorescence observation performed on a predetermined sample, and under a predetermined observation condition; an input unit via which inputs the type of sample to be observed and the type of fluorescent substance to be used; an assistance-display generating unit that reads sample state information and an observation condition of fluorescence observation performed on the same type of sample as the type of sample input, using the same type of fluorescent substance as the type of fluorescent substance, and generates an assistance display showing the sample state information in relation to the two or more parameters included in the observation condition; and a display unit that displays the generated assistance display.