公开(公告)号：US10379327B2

公开(公告)日：2019-08-13

申请号：US15608342

申请日：2017-05-30

Applicant: OLYMPUS CORPORATION

Inventor： Hidenori Tsuboi

IPC: G02B21/00 ,  G01N21/64 ,  G02B21/36

Abstract: Provided is a scanning microscope including a pinhole array disk having a plurality of pinholes restricting a light flux of illumination light for irradiating a sample, the plurality of pinholes being disposed so as to form an array about a center axis; a rotational driving unit rotating the pinhole array disk about the center axis; an objective lens irradiating the sample with the illumination light that has passed through the pinholes and collecting fluorescence from the sample to cause the fluorescence to enter the pinholes; a camera acquiring an image of the sample by repeatedly capturing the fluorescence that has passed through the pinholes; and a stimulation optical system irradiating the sample with stimulation light, wherein the system includes a stimulation-timing generating unit decreasing the intensity of the stimulation light during exposure periods of the camera and increasing the intensity of the stimulation light during periods between exposure periods.

公开(公告)号：US10261299B2

公开(公告)日：2019-04-16

申请号：US16102840

申请日：2018-08-14

Applicant: OLYMPUS CORPORATION

Inventor： Kentaro Imoto ,  Ryusuke Tanaka ,  Hidenori Tsuboi

IPC: G01N21/64 ,  G02B21/00

Abstract: Provided is an image-acquisition apparatus including: a scanning portion that scans illumination light emitted from a light source; an optical system that focuses the scanned illumination light on a sample, while collecting signal light beams generated at the individual scanning positions on the sample; a detector that detects the collected signal light beams and that generates detection signals thereof; a signal controller that is configured to generate periodic signals that are repeated at a predetermined period; a light controller that is configured to temporally control a position or an intensity of the illumination light in accordance with the generated periodic signals; and a computer that is configured to process, in accordance with the generated periodic signals, the detection signals generated, wherein the computer is provided with a frequency shifter for shifting frequencies of the detection signals, and an integrator that integrates the shifted detection signals at an integration time.