Azatriangulenium Salts As Pet-Quenched Fluorescent Probes
    6.
    发明申请
    Azatriangulenium Salts As Pet-Quenched Fluorescent Probes 审中-公开
    氮化三环盐作为宠物淬火荧光探针

    公开(公告)号:US20160251369A1

    公开(公告)日:2016-09-01

    申请号:US15028558

    申请日:2014-10-24

    摘要: The present invention relates to a new class of substituted aza-triangulenium fluorescent dyes having a hydroxy group attached to an aryl as quenching group. The new substituted aza-triangulenium fluorescent dyes may be attached to a linker, conjugated to carrier molecule such as e.g. a protein, a nucleic acid, a lipid, or a saccharide, or deposited or immobilised on solid support materials. The substituted aza-triangulenium fluorescent dyes are useful for various purposes, including use in sensors for monitoring or determining the concentration of analytes, such as H+(pH), Na+, K+, Ca2+, O2, CO2, H2O2, ionic strength, redox potentials, metal ions, and metabolites.

    摘要翻译: 本发明涉及一类新的具有连接到芳基作为猝灭基团的羟基的取代的氮杂 - 三联体荧光染料。 新的取代的氮杂三环脲荧光染料可以连接到与载体分子结合的接头上, 蛋白质,核酸,脂质或糖类,或者沉积或固定在固体支持材料上。 取代的氮杂三环脲荧光染料可用于各种目的,包括用于监测或测定分析物浓度的传感器,如H +(pH),Na +,K +,Ca2 +,O2,CO2,H2O2,离子强度,氧化还原电位 ,金属离子和代谢物。

    SOLUTION PHASE HOMOGENEOUS ASSAYS
    9.
    发明申请
    SOLUTION PHASE HOMOGENEOUS ASSAYS 审中-公开
    解相位均质测定

    公开(公告)号:US20150212005A1

    公开(公告)日:2015-07-30

    申请号:US14677524

    申请日:2015-04-02

    IPC分类号: G01N21/76

    摘要: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.

    摘要翻译: 公开了用于确定疑似含有分析物的样品中的分析物的方法,试剂,试剂盒和系统,其中所有试剂都可溶于水溶液。 一种测定方法包括在如下条件下处理怀疑含有分析物的样品,使得如果存在分析物,则使活化剂与化学发光化合物反应构型以使其活化。 样品也用试剂处理以减少与分析物无关的信号。 最后,用触发溶液处理样品,从而产生来自活化化学发光化合物的光。 没有试剂与表面或其他固相相关。